Genetic variations of the CDC2L2 gene are associated with type 2 diabetes in a Han Chinese cohort

OBJECTIVE: The purpose of the present study was to study the potential association of CDC2L2 variations with type 2 diabetes (T2D). METHODS: SNPs (single nucleotide polymorphisms) were extensively screened across the CDC2L2 gene by the site-specific PCR method ARMS (Amplification Refractory Mutation System). The identified novel polymorphisms were further evaluated in a Han Chinese cohort comprising of 467 patients with diabetes and 569 nondiabetic controls. In addition, 76 parent-offspring trios were also included in this association study.The case-control and TDT/sibTDT studies are applied for association analysis in this study. RESULTS: Seven loci (rs1059831, SNP33, rs7528782, SNP11, SNP36, rs11488590 and SNP30) were shown to be significantly associated with T2D in unrelated individuals (p < 0.05). When individuals were stratified by age, sex and body mass index (BMI), the SNP11 was shown to be strongly associated with female patients with T2D, patients whose age was over 45 years and individuals whose BMI was less than 23 (p = 0.018, 0.011 and 0.0089, respectively). However, it was not replicated in the family-based TDT/sibTDT analysis (p = 0.085, OR = 0.63 (CI 95% 0.34-1.06)). CONCLUSION: Our data suggested that the CDC2L2 gene may contribute to the susceptibility of type 2 diabetes in the northern Han Chinese population, but further studies are needed to replicate these findings.     

基层疾控机构文档管理现况调查与评价

目的调查和分析目前基层疾控机构文档管理工作现况并提出整改建议。方法对湖北省某中心城市的基层疾控机构实地评估,制定考核标准和考核评分表,对各基层疾控机构考核评定。评估共进行两次。结果第一次评估是发现各基层疾控机构文档管理现况相对较差,文档存放混杂,文档管理思路不正确;两次评估对比显示,第二次评估得分明显高于第一次,基层疾控机构文档管理工作明显改善。结论目前基层疾控机构的文档管理缺乏正确的思路和管理方法,也未引起足够重视。应加强引导培训,促进基层疾控机构的文档管理工作。     

宫颈癌中CDC6、Survivin蛋白的表达与HPV16/18感染的相关性

[目的]研究CDC6、Survivin在宫颈癌组织中的表达及其与HPV16/18感染的相关性,以探讨宫颈癌的发生机制,进一步寻找有利于宫颈癌诊断的新的分子标志物,以指导其诊断与预后.[方法]采用免疫组织化学方法对50例宫颈癌、20例宫颈上皮内瘤样病变CIN1、20例CINⅡ-Ⅲ、20例正常宫颈组织进行CDC6、Survivin的检测,同时采用PCR技术检测HPV16/18感染情况.[结果](1)在宫颈癌组织中CDC6和Survivin表达的阳性率高于CIN和正常宫颈组织,差异有统计学意义(均P＜0.05),且CDC6和SurvMn阳性表达率与病理分级、淋巴结转移有关,差异有统计学意义(均P＜0.05),CDC6、Survivin阳性表达率均与年龄分组、临床分期无关(均P＞0.05). (2)HPV16/18在正常宫颈组织、CIN和宫颈癌中的阳性率逐渐升高,差异有统计学意义(P＜0.05),但与年龄、临床分期、病理分级、淋巴转移无关(均P＞0.05). (3)在宫颈癌组织中CDC6与HPV16/18感染呈正相关(P＜0.05),Survivin与CDC6、HPV16/18感染呈正相关(均P＜0.05).[结论]宫颈上皮内瘤样病变及宫颈癌鲴织中CDC6、Survivin表达的改变可能与HPV16/18感染有关,且互相作用,共同影响CIN的发展及宫颈癌的发生.     

天津市某疾控中心工作人员不同进餐地点膳食结构和营养状况

目的研究某疾病预防控制中心（疾控）工作人员不同进餐方式膳食结构和营养状况。方法采用回顾法计算3种进餐方式特定时间内的各种食物摄入量。结果天津市某疾控工作人员每标准人日鱼虾类、畜禽肉、蛋、豆类、坚果、油脂、食盐摄入量以在饭店进餐最高;能量、蛋白质、脂肪、大部分维生素和矿物质摄入量也是饭店进餐方式最高。能量蛋白质、脂肪来源由高到低的进餐地点为饭店、家庭、食堂;能量碳水化合物来源由高到低的进餐地点为食堂、家庭、饭店。结论天津市某疾控工作人员膳食结构和营养状况在不同进餐地点,以家庭就餐较好。3种进餐地点共同存在蔬菜、维生素C摄入偏少,食盐、Na摄入偏多的问题。     

Chromosome 1p21 deletion is a novel prognostic marker in patients with multiple myeloma

The combination of fluorescence in situ hybridization with cytoplasmic light chain detection identified chromosome 1p21 deletion in 18 (20%) of 87 patients with multiple myeloma. 1p21 deletion was associated with higher serum calcium level, 13q deletion, and t(4;14). Patients with 1p21 deletions had a significantly shorter progression-free survival (PFS) (median 10.5 vs. 22.3 months, P = 0.0002) and shorter overall survival (OS) (median 33.9 months vs. not reached, P = 0.002) than those without 1p21 deletions. On multivariate analysis, which included deletions of 13q, TP53, t(4;14) and CKS1B amplification, 1p21 deletion remained as an independent risk factor for PFS (P = 0.01) and OS (P = 0.04).     

The impact of intra- and postoperative fluid balance in pancreatic surgery - A retrospective cohort study

Background/objectivesThe aim of this study was to evaluate the impact of perioperative fluid administration in pancreatic surgery.MethodsPatients who underwent pancreatic resections were identified from our institution's prospectively maintained database. Fluid balances were recorded intraoperatively and at 24hr postoperatively. Patients were stratified into tertiles of fluid administration (low, medium, high). Adjusted multivariable analysis was performed and outcome measures were postoperative complications.ResultsA total of 211 patients were included from 2012 to 2017. Complication rates were POPF(B/C) 19.4%, DGE(B/C) 14.7%, PPH(C) 10.0% and CDC ≥ IIIb 26.1%. In multivariable analysis, high perioperative fluid balance was an independent risk factor associated with POPF (OR = 10.5, 95%CI 2.7–40.7, p = .001), CDC (OR = 2.5, 95%CI 1.2–5.3, p < .002), DGE (OR = 2.3, 95%CI 1.0–5.2, p = .017), PPH (OR = 6.7 95%CI 2.2–20.0, p = .038) and reoperation (OR = 3.1, 95%CI 1.6–6.2, p = .006). In multivariable analysis with intraoperative and postoperative fluid balances as separate predictors, intraoperative (OR = 2,5, 95%CI 1.2–5.5, p = .04) and postoperative fluid balance (OR = 2.5, 95%CI 1.2–5.5, p = .02) were predictors of POPF. Postoperative fluid balance was the only predictor for mortality (OR = 4.5, 95%CI 1.0–18.9, p = .041) and predictor for CDC (OR = 2.0, 95%CI 1.0–4.0, p = .043) and OHS days (OR = 6.9, 95%CI 0.03–13.7, p = .038).ConclusionsHigh postoperative fluid balance in particular is associated with postoperative morbidity. Maintaining a fluid-restrictive strategy postoperatively should be recommended for patients undergoing pancreatic surgery.     

Cervicitis in adolescents: do clinicians understand diagnosis and treatment?

Background

Cervicitis is widespread, but no studies have examined cervicitis in accordance with established guidelines for diagnosis and treatment. Study objectives were to describe adherence to Centers for Disease Control and Prevention guidelines for diagnosis and treatment of cervicitis within an adolescent population and to compare factors associated with adherence to guidelines in a primary care setting and the Emergency Department.

Methods

Data were collected as part of a retrospective chart review of evaluation, diagnosis, and treatment of STI in adolescent women in an outpatient setting. Participant charts were eligible for review if they were 12–21 years of age and were given an ICD-9 and chart diagnosis of cervicitis. Two primary outcome variables: meeting cervicitis guidelines and correct treatment among those meeting cervicitis guidelines (no/yes) were utilized; the study controlled for age, race, venue, past infection with chlamydia or gonorrhea.

Results

Subjects (n = 365) were examined for the primary outcome variables and 75.1% (274/365) met at least one criterion for cervicitis. Of these, 166 (60.9%: 166/274) subjects were found to meet criteria for cervicitis alone, versus subjects meeting criteria for both cervicitis and pelvic inflammatory disease (PID) (39.4%: 108/274). The majority, 89.3%, (326/365) were treated for both chlamydia and gonorrhea, but only 64.7% (211/326) were treated correctly for both infections.

Conclusions

Our findings suggest that knowledge deficits exist in diagnosis and treatment of cervicitis in adolescent patients and in differentiating between cervicitis and PID. Educational tools, simulated patient exercises, and order sets may be warranted for quality improvement to allow for improved care of this at risk sexually active population.     

周期蛋白6在DNA复制、细胞周期检测点和肿瘤发生发展中的作用

周期蛋白6(CDC6)是组成前复制复合物(Pre-RC)的主要蛋白之一,控制细胞从G1期进入S期,同时也参与激活和维持有丝分裂S-M期检测点机制.最近的研究发现其也具有原癌基因的特性,并在人多种肿瘤细胞中存在高表达,对肿瘤发生发展起重要作用.CDC6致癌机制可能与INK4/ARF连结物信号途径和(或)某些替代机制有关.     

The association between diabetes mellitus,sugar-sweetened beverages,and tooth loss in adults: Evidence from 18 states

Background

Sugar-sweetened beverages (SSBs) are dietary sources of sugar that are factors in caries development and tooth loss. Dietary sugar also is linked to diabetes mellitus (DM). There is limited research related to SSBs and tooth loss in people with DM. The authors investigated the association between SSBs and tooth loss as it related to the presence or absence of DM.