串联质谱检测干血滤纸片中氨基酸质控分析

目的通过对近3年的质控结果进行分析，探讨影响检测结果的因素及应对措施。方法美国疾病控制和预防中心（CDC）每年提供4批氨基酸质控干血滤纸片，利用串联质谱技术检测干血滤纸片中氨基酸水平。以美国CDC检测结果为标准，分析本实验室氨基酸质控结果偏差及其他实验室质控结果（213个实验室的均值）偏差之间的差异。结果本实验室每次质控结果所有氨基酸均在美国CDC检测结果95％可信区间内，且每一批结果的临床评价与美国CDC质控临床评价完全一致。本实验室质控结果偏差与其他实验室质控结果偏差比较，苯丙氨酸、亮氨酸、甲硫氨酸、酪氨酸和缬氨酸均无显著性差异，瓜氨酸有显著性差异（Z=-2．945P〈0．005）。结论本实验室氨基酸质控结果，达到美国CDC的质控要求，瓜氨酸检测值偏低，其阳性判断切割值设定也相应降低。     

Identification and Phylogeny of a Non-specific Endonuclease Gene of White Spot Syndrome Virus of Shrimp

White spot syndrome virus (WSSV) is a taxonomically unclassified virus which causes a disease in shrimps worldwide. A 936 bp long open reading frame (ORF) was found on a 7.2 kb HindIII fragment of the DNA genome of WSSV located adjacent to the ribonucleotide reductase small subunit gene. This putative ORF showed homology to prokaryotic and eukaryotic endonucleases, which contain a non-specific endonuclease motif. Alignment with viral and eukaryotic endonuclease ORFs revealed that most catalytically and structurally important amino acid residues were present in the putative WSSV non-specific endonuclease gene. An unrooted parsimonous phylogenetic tree of non-specific endonucleases indicated that the WSSV ORF was located in a well bootstrap supported clade containing only arthopods, including one of WSSV's natural hosts, Penaeus japonicus. A similar conjunction was found for the only other viral homologue, present in Fowlpox virus, which was also found in a well bootstrap-supported clade with its natural host, Gallus gallus. This clustering of virus and host suggests that both WSSV and Fowlpox virus may have acquired their nuclease genes from their respective natural hosts. Because the motif for non-specific nucleases is found in only two viruses, this gene cannot be used to clarify the taxonomic position of WSSV. However, the presence of this type of nuclease rarely found in viruses adds a novel feature to WSSV.     

Clearing a blind spot in knee arthroscopy: popliteal bursa

Each arthroscopic portal to the knee has a blind area that cannot be inspected directly. In particular, visualization of the posterior compartment is difficult to access. Although arthroscopic procedures for the posterior compartment of the knee joint have been developed through posteromedial, posterolateral and posterior trans-septal portal, the popliteal bursa remains as an area that is difficult to access and manipulate. We report a surgical procedure that can examine one of the blind spots in knee arthroscopy, popliteal bursa.     

国内旅游地和风景区卫生状况评价

卫生状况的好坏直接影响着旅游业的发展，恶劣的卫生状况使国内外的旅游者闻而怯步。本文主要从旅游地、风景区的环境卫生、虫媒鼠患、饮食卫生和疫源性疾病等方面对国内旅游地、风景区的卫生状况加以评价。从总体看，国内旅游地、风景区的卫生状况正从初、中级向高级。从不注重，注重不多到关注较多的方向发展。     

The ‘Hyperactive’ Child

A new, more apt designation, attentional deficit disorder (ADD), may help dispel some of the myths that have surrounded the “hyperactive” child. An abnormally high level of physical activity is a common but not universal finding in these children but is not their central problem. In most affected children, early diagnosis and specific drug treatment can ward off the often disastrous psychiatric and social consequences of ADD.     

利用国外政府贷款购置医疗设备应注意的问题

本文主要介绍了我院利用国外政府贷款购置医疗设备的背景、步骤以及需要注意的问题。     

用cDNA:RNA斑点杂交法检测肠道病毒RNA的实验研究

用Biotin标记的Coxsackie B3病毒cDNA为探针,以25种肠道病毒标准毒株为检测病毒,建立了检测肠道病毒基因RNA的斑点杂交方法。该方法的检测敏感性为50～80TCID_(50)斑点,操作较简便,试剂稳定安全、可长期保存。所使用的pCB Ⅲ/35.51对肠道病毒有很广的杂交谱;pCBⅢ/29在严格条件下,仅与Coxsackie B3病毒RNA反应。因此,这些探针所具有的杂交特性很适合作为临床标本中肠道病毒的检测探针。     

Comparative study of the sensitivity and specificity of the zinc and acid phosphatase spot tests for the detection of seminal stains

Summary The sensitivity and specificity of a zinc spot test for the detection of semen were compared with those of an acid phosphatase detection method. As screening techniques both tests were found to be very sensitive, but the zinc test was more specific and was more reliable in older and especially in deteriorated specimens. It is concluded that the zinc spot test deserves at least the same place as the acid phosphatase test in the primary investigation of suspected semen stains and might well be the test of choice in older and poorly preserved stains.     

1个感染性螺点灭螺后11年的螺情动态观察

目的 探索云南大山区阳性螺点灭螺后螺情动态变化规律，为今后螺情的监测及控制血吸虫病提供依据。方法 对云南山区1个阳性螺点1次定点灭螺后的螺情进行了11年的考核观察。每次均通过环境抽查设框调查螺情并解剖钉螺以求出钉螺感染血吸虫的自然感染率。结果 11年内共考核10次，结果显示灭螺后3年内查不到活螺，但第4年开始又在原螺点内找到钉螺，至第5年钉螺密度恢复到灭螺前水平，并有感染螺出现，且在未进行第2次灭螺的情况下，其疫源性保持5年未变。结论 阳性螺点的长期监测及消灭对于阻断云南山区血吸虫病的流行具有重要意义。阳性螺点的监测可每2－3年进行1次。