新斯的明引起大鼠神经肌接头突触后膜持续性去极化特点

采用大鼠膈肌不均匀牵张肌肉标本,观察可逆性胆碱酯酶抑制剂新斯的明引起神经肌接头突触后膜持续性去极化特点. 结果发现,新斯的明（2.5-5 μmol·L^-1）浴槽内给药引起的持续性去极化（SD）时程比压力给药（1 mmol·L^-1,32 kPa, 2-5 s）时长,结果提示压力给药引起的SD时程仅相当于浴槽内低浓度药物所引起者. 在本实验条件的多数情况下,压力给药引起SD的平台期,复极期以及复极后的一段时间内出现频率显著增高的小终板电位（mEPP）,并且这种高频率的mEPP只伴随SD的产生而出现. 而在浴槽内给予高浓度新斯的明引起的SD过程中,mEPP显著减少. 结果提示,可逆性胆碱酯酶抑制剂新斯的明在低浓度时引起的SD过程中,不仅突触后膜的烟碱受体并没有完全失敏,而且突触前膜自发释放ACh显著增加.     

半夏泻心汤对小鼠胃排空的影响

半夏泻心汤对正常小鼠胃排空无影响,能拮抗阿托品、芬氟拉明、多巴胺所致的小鼠胃排空抑制,但对左旋麻黄碱和吗啡所致抑制无拮抗作用,同时对新斯的明和胃复安引起的小鼠胃排空亢进有拮抗作用。提示本方对偏抑或偏亢机能状态下的胃排空具有双向调节作用,其作用机制可能与胆碱受体、多巴胺受体及5－羟色胺受体系统有关。     

Shock waves as treatment of mouse myofascial trigger points

Introduction

An abnormal increase in spontaneous neurotransmission can induce subsynaptic knots in the myocyte called myofascial trigger points. The treatment of choice is to destroy these trigger points by inserting needles. However, 10% of the population has a phobia of needles, blood, or injuries. Therefore, the objective of this study is to verify the usefulness of shock waves in the treatment of myofascial trigger points.

Methods

Two groups of mice have been developed for this: healthy muscles treated with shock waves; trigger points affected muscles artificially generated with neostigmine and subsequently treated with shock waves. Muscles were stained with methylene blue, PAS-Alcian Blue, and labeling the axons with fluorescein and the acetylcholine receptors with rhodamine. Using intracellular recording the frequency of miniature endplate potentials (mEPPs) was recorded and endplate noise was recorded with electromyography.

Results

No healthy muscles treated with shock waves showed injury. Twitch knots in mice previously treated with neostigmine disappeared after shock wave treatment. Several motor axonal branches were retracted. On the other hand, shock wave treatment reduces the frequency of mEPPs and the number of areas with endplate noise.

Discussion

Shock waves seem to be a suitable treatment for myofascial trigger points. In the present study, with a single session of shock waves, very relevant results have been obtained, both functional (normalization of spontaneous neurotransmission) and morphological (disappearance of myofascial trigger points). Patients with a phobia of needles, blood, or injuries who cannot benefit from dry needling may turn to noninvasive radial shock wave treatment.