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101.
目的:观察兔骨髓基质细胞体外培养的生长行为及增殖、分化情况,探讨由骨髓分离培养神经干细胞的可行性和有效性。方法:无菌条件下行骨穿术,密度梯度离心分离获取骨髓基质细胞,利用多种增殖、分化诱导因子和神经干细胞培养液进行培养、分化诱导,用免疫细胞化学方法进行鉴定。结朵:骨髓基质细胞在体外培养中能形成NESTIN抗原阳性的细胞克隆团,进一步分化,部分细胞胞体增大并出芽,逐渐发育成为较成熟的长突起细胞,长突起相互连接、交织成网并建立纤维联系。结论:骨髓基质细胞具有较强的自我更新能力和多向分化潜能,易于取材、体外培养和增殖,在一定诱导条件下可以生成神经干细胞。 相似文献
102.
三氧化二砷与维甲酸联合作用在NB4,MR2细胞系中的体外研究 总被引:2,自引:0,他引:2
目的:观察三氧化二砷(As2O3)和全反式维甲酸(ATRA)联合作用对NB4,MR2不同亚型急性早幼粒白血病细胞株,诱导分化、增殖、凋亡的影响。方法:以NB4,MR2为体外模型,利用细胞生长曲线、台盼蓝拒染法、MTT法、NBT、细胞形态Wrigh‘s-Gimesa染色,观察细胞增殖、分化、凋亡的相互作用。结果:NB4细胞株:0.5μmol/L As2O3与10^-6mol/L ATRA对细胞增殖、分化、凋亡呈现拮抗,而0.5μmol/L As2O3与(10^-7--10^-8mol/L)ATRA呈现协同;MR2细胞株:0.5μmol/L As2O3与10^-6mol/L ATRA对细胞增殖、分化、凋亡呈现协同。结论:As2O3与全反式维甲酸协同效应呈现不同细胞、剂量的特异性,MR2细胞株协同效应明显强于NB4细胞株。 相似文献
103.
MARION FRANKENBERGER BARBARA HOFMANN BERTOLD EMMERICH CHRISTOPH NERL RETO A. SCHWENDENER & H.W. LÖMS ZIEGLER -HEITBROCK 《British journal of haematology》1997,98(1):186-194
The vitamin D3 derived hormone 1,25 (OH)2 vitamin D3 (1,25 D3 ) is able to induce growth arrest and differentiation in myelomonocytic leukaemia cells. In order to allow for specific delivery to leukaemic cells the lipophilic compound was incorporated into the lipid membranes of liposomes. Liposomal 1,25 D3 reduced proliferation as measured by 3 H-thymidine incorporation in HL60 leukaemia cells by up to 60%. When liposomes were prepared at different concentrations of 1,25 D3 65% inhibition was achieved at 48 n M . The MC 1288 stereoisomer of 1,25 D3 was more potent and had the same activity at 48 n M .
The effect of the liposomal compounds was specific to myeloid cells as they reduced proliferation in myelomonocytic HL60, monoblastic U937 and monocytic Mono Mac 6 cells but not in the T-cell lines Jurkat and Molt 4.
The antiproliferative effect of liposomal 1,25 D3 was associated with an induction of differentiation since treated HL60 cells showed a monocytic morphology, increased expression of CD14 and decreased expression of CD33.
When peripheral blood leukaemic cells from M4 and M5 acute myeloid leukaemia (AML) patients were admixed with liposomal compounds an antiproliferative effect was seen in all five cases, including the two cases where free compounds led to enhanced growth. Liposomal delivery of 1,25 (OH)2 vitamin D3 may offer a novel approach to treatment of myelomonocytic leukaemia. 相似文献
The effect of the liposomal compounds was specific to myeloid cells as they reduced proliferation in myelomonocytic HL60, monoblastic U937 and monocytic Mono Mac 6 cells but not in the T-cell lines Jurkat and Molt 4.
The antiproliferative effect of liposomal 1,25 D
When peripheral blood leukaemic cells from M4 and M5 acute myeloid leukaemia (AML) patients were admixed with liposomal compounds an antiproliferative effect was seen in all five cases, including the two cases where free compounds led to enhanced growth. Liposomal delivery of 1,25 (OH)
104.
用六亚甲基二乙酰胺(HMBA)作为分化诱导剂,对人粘液表皮样癌细胞系MEC-1细胞中角蛋白进行染色,结果发现高分子量角蛋白在被诱导细胞中含量明显增高。分光光度仪检测角蛋白含量,诱导组x±s为218±51,对照组为149±47,两组有显著差异(P<001),这可能与细胞分化有关。 相似文献
105.
The sudden appearance of prolactin-releasing cells during the early postnatal period of the rat is initiated by a small milk-borne
peptide. Depriving newborn rats of this early milk factor severely retards mammotrope differentiation during the neonatal
period. In the present work, we extend our study of early milk deprivation to the adult. To this end, newborn litters were
crossfostered onto mothers that had given birth the same day or one week earlier in order to deprive pups in the latter group
of early milk. At 5, 15, and 30 d of age, rats deprived of such milk had decreased percentages of mammotropes (as measured
by reverse hemolytic plaque assay, RHPA) when compared to nondeprived animals (P<0.05). By 45 d, the percentage of mammotropes was similar for the two crossfostered groups (P>0.1) and this persisted through d 60. Subsequently, we assessed the secretory capacity of mammotropes from 60-d old rats
to secretagogues and found that early milk deprivation had no effect on basal prolactin release (P>0.1), but that it augmented
hormone secretion evoked by thyrotropin-releasing hormone (TRH, 100 nM; P<0.01). The inhibitory response to dopamine (DA; 1 μM) and the stimulatory response to angiotensin II (AGII; 100 nM) were not altered by early milk deprivation (P>0.1). Taken together, these results demonstrate that factors in milk from early lactation are required for normal mammotrope
differentiation, and that the delay induced by early milk deprivation leads to altered secretory function of mammotropes in
adult animals. 相似文献
106.
对甲状腺机能亢进症(甲亢)患者进行了临床辨证分型,同步观察甲皱微循环及检测TT_3 TT_4 FT_4I、吸~(131)碘率,探讨它们之间的关系。结果表明甲亢患者微循环积分明显高于对照组(P<0.01),但不同类型甲亢的血淤情况亦不相同。心肝火旺型的微循环积分低于气滞痰凝型和血瘀型,但TT_3、TT_4、FT_4I明显增高;气滞痰凝型居中;血淤型的微循环积分明显增高,但TT_3、TT_4、FT_4I低于其他两型。吸~(131)碘率三型间无差异(P>0.05)。 相似文献
107.
108.
Neuronal migration and dendritic maturation of the medial cerebellar nucleus in rat embryos: an HRP in vitro study using cerebellar slabs 总被引:2,自引:0,他引:2
The morphological maturation of medial nuclear neurons of fetal rat cerebella was studied using an in vitro assay. Neurons of this nucleus were identified in isolated preparations of rhombencephalon between embryonic days 16 and 20 (E16-E20) by the intracerebellar decussation of their outgrowing axons within the uncinate fascicle. A small crystal of horseradish peroxidase (HRP) applied either in the region containing the inferior cerebellar peduncle or, preferably, in the lateral cerebellum retrogradely labeled contralateral medial nuclear neurons. In the youngest embryos (E16-E17), HRP-marked neurons were situated rostrally at the dorsal surface of the cerebellum. By E18, the cell mass containing labeled neurons had shifted in a rostrocaudal and dorsoventral direction and finally reached the adult position in E19-E20 embryos. Dendritic differentiation of these neurons followed a similar positional gradient, closely corresponding to the pattern of temporal development. From the most immature monopolar forms located dorsally to the virtually adult stellate neurons in a ventral position, it was possible to trace a continuum of intermediary forms grouped into six well-defined stages. Immature monopolar cells first became transversely bipolar. Then, they changed orientation, assuming a longitudinal radial direction. During this stage, neurons sank into the cerebellar parenchyma. As they reached their final destination, these neurons gradually developed dendrites which radiated from the cell body in an adult-like pattern. It is concluded that the medial nuclear neurons occupy a superficial dorsal position in early phases of cerebellar ontogeny, thereafter undergoing a second, inward migration. The main stages of neuronal dendritic differentiation occur between E16 and E20, indicating that the ingrowth of afferent in puts to the medial nucleus most probably occurs rather early and is concomitant with dendritic development. 相似文献
109.
We have devised a medium which supports the continuous growth of hepatocytes without losing their replicative potential and differentiation capacity for a longer period. The medium HCGM, contains four key substances in addition to foetal bovine serum. They are epidermal growth factor, nicotinamide, ascorbic acid 2-phosphate and dimethylsulphoxide. When a non-parenchymal cell fraction containing small hepatocytes and non-parenchymal cells was cultured in HCGM, small hepatocytes grew clonally and differentiated into cells expressing either mature hepatocyte marker proteins or biliary cell marker proteins. Thus, for the first time, we showed the presence of a small compartment of bipotent and highly replicative clonogenic hepatocytes in the rat adult liver. HCGM also supported the growth of stellate cells (Ito cells) which were in the original preparation, suggesting the important role of stellate cells for the successful cultivation of hepatocytes. Together, these results suggest that a microenvironment is produced as a result of cooperative interactions between hepatocytes and stellate cells: one which stimulates the growth and differentiation of clonogenic hepatocytes. 相似文献
110.
Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: a whole-mount immunocytochemical study 总被引:5,自引:0,他引:5
Rhombencephala from rat embryos were processed as whole-mounts for immunocytochemical detection of monoaminergic cell populations, using antibodies to tyrosine hydroxylase (TH) and serotonin (5-HT). Specific advantages of the whole-mount technique over the classical serial-section method were that even isolated immunoreactive (IR) cells could be detected easily, and three-dimensional relationships could be ascertained without the need for serial reconstruction. Embryos between embryonic days (E) 12 and 16 (the day following nocturnal mating being considered as E1) were used in this study. Both TH and 5-HT immunoreactivities were already detectable at E12, even in the smallest embryos (crown-rump length: 6 mm), but there was a striking difference in the number and regional distribution of these two types of IR cells. TH was expressed in several cell groups located in the rostral rhombencephalon (the presumed anlage of the A4-7 complex) as well as in the caudal rhombencephalon (the presumed anlagen of groups A1-2 and C1-3), whereas 5-HT was expressed in very few cells located near the rostral border of the rhombencephalon (presumed anlage of the B4-9 complex). Although the three-dimensional distribution of the TH-IR cell groups underwent some modifications during the period studied, its general pattern remained relatively stable after E12. This contrasted with the sequential appearance of the 5-HT-IR cell groups and their spatial transformations during this period. Using the rhombencephalic isthmus as a landmark, we found that conspicuous 5-HT-IR fibre bundles penetrated into the mesencephalon from E13 onwards, but that the 5-HT IR cell bodies were exclusively located caudal to the borderline between the mesencephalon and the rhombencephalon (the rhombencephalic isthmus). We therefore suggest the term "rostral rhombencephalic raphe nuclei" for the rostral 5-HT cell groups instead of "mesencephalic raphe nuclei," which is a misnomer. Close spatial association between TH and 5-HT-IR elements was observed mainly in the caudal rhombencephalon, where 5-HT-IR fibres coursed through an area containing numerous TH-IR cell bodies (the presumed anlagen of groups A1-2 and C1-3). 相似文献