食管、贲门癌围手术期细胞免疫功能动态变化的临床研究

目的：探讨食管、贲门癌全身、局部细胞免疫功能状态及生物治疗的可行性。方法：采用免疫组化观察分析５０例食管、贲门癌局部肿瘤浸润淋巴细胞（ＴＩＬ）、肿瘤相关性巨噬细胞（ＴＡＭ）的分布特征，采用ＥＬＩＳＡ法和硝酸还原酶法分别地体内可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）表达和一氧化氮（ＮＯ）生成水平进行动态检测。结果：１）癌周物浸润密度大于癌果间质；２）２术前血清ｓＩＬ－２Ｒ水平高于正常对照组，根治术后ｓ     

亚急性低剂量铅暴露对大鼠细胞免疫功能的影响

为探讨亚急性低剂量铅暴露对大鼠细胞免疫功能的影响，将幼年ＳＤ大鼠分成６组，给予含不同剂量铅的饲料８周，观察铅暴露后大鼠血铅、Ｔ细胞亚群、血清肿瘤坏死因子（ＴＮＦ－α）和脾脏超微结构的变化。８周后各剂量铅暴露组血铅均高于正常对照组（Ｐ＜０．０１），并且随着铅剂量的增加而呈阶梯状升高。各铅暴露组Ｔ细胞亚群分布异常，ＣＤ４明显减少，ＣＤ４／ＣＤ８比例下降，ＣＤ８各组间差异显无显著性；血清ＴＮＦ－α分泌异     

男必康茶的药效学研究

目的 研制开发治疗前列腺增生新药男必康茶。方法 选用抑制增生、抗菌、解热、利尿及增强免疫的方法，对其进行药效学研究。结果 男必康茶７．５ｇ／ｋｇ灌胃给药能使丙酸睾丸素造成的大、小鼠前列腺增生的前列腺指数降低，减轻大鼠棉球肉芽肿的质量；增加金黄色葡萄球菌、大肠杆菌及甲链球菌所致小鼠死亡的动物存活数；提高小鼠ＨＣ５０和碳末廓清指数；而对角叉菜胶引起的大鼠肛温升高及大鼠的尿量无明显影响。结论 男必康茶具     

人血浆纤维连接素的提纯及其抗血清制备

本文报道了用明胶-cNBr-Sepharose4B亲和层析法与Sephadex G200滤过层析法相结合提取人血浆Fn,操作简便,抗原纯度高。采用微量多点注射免疫新西兰大白兔,制备了兔抗人血浆Fn抗血清,能与人Fn抗原呈特异性反应,也能与其它动物的Fn抗原呈交叉反应,可用于测定血浆中Fn含量和免疫组织化学研究。     

Induction of Lymphokine-Activated Killer Activities in Peripheral Blood Lymphocytes and Regional Lymph Node Lymphocytes Against PC-9 Cultured Adenocarcinoma and Autologous Pulmonary Adenocarcinoma Cells

The cytotoxic activities, of the peripheral blood lymphocytes(PBL) and regional lymph node lymphocytes (LNL) generated byincubation with recombinant IL-2 (rIL-2) in vitro, have beenstudied to assess their effects on autologous pulmonary adenocarcinomacells from 21 patients with lung cancer. The lymphokine-activatedkiller (LAK) activities of PBL were 22.9±14.8% for autologouspulmonary adenocarcinoma cells and 44.9±20% for PC-9cells. A previous coculture of autologous tumor cells with PBL(ATS-LAK) showed no Increase in induction of cytotoxicity toautologous tumor cells. The LAK activities of LNL were significantlylower than those of PBL. The cytotoxicities of rIL-2 activatedlymphocytes seemed lower in well differentiated pulmonary adenocarcinomathan in moderately and poorly differentiated adenocarcinoma,and higher in patients with lymph node metastasis than in thosewithout; they appeared, however, not to be related to clinicalstage or degree of infiltration of T-zone histiocytes into thecancer stroma. The cytotoxicity to autologous tumor cells wasnot significantly different in the presence of the Ia antigenexpressed on tumor cells, but the cytotoxicity to la positivePC-9 cells was significantly higher for rIL-2 activated lymphocytesfrom patients with lung cancer cells which were la positivethan from those where they were la negative. The presence ofla antigen on tumor cells seemed important for antitumor activity.     

膀胱癌患者外周血树突状细胞免疫状态变化及其临床意义

目的探讨膀胱癌患者外周血中树突状细胞（DC）的免疫功能状态变化及其与膀胱癌发生的关系。方法检测15例临床确诊的中晚期膀胱癌患者外周血DC表面CD1a、CD83表达水平及免疫诱导T淋巴细胞增殖的能力，并与10名健康成人比较。结果膀胱癌患者DC表面CDla及CD83表达水平（分别为57．4±1．6，72．1±1．1）明显低于正常对照组（64．9±1．4，80．6±1．2，P〈0．05）；其体外诱导T细胞增殖的能力亦明显低于正常对照组（P〈0．05）。结论膀胱癌患者DC存在免疫功能缺陷可能是膀胱癌逃避宿主免疫监视的重要原因之一。     

Is there an established role for immunotherapy for any of our surgical cancers?

Despite major advances in the management of the patient and in surgical and radiation therapy, cancer deaths continue to occur secondary to widespread metastases. Therefore, systemic treatments in the form of chemotherapy, hormonal therapy, and immunotherapy are constantly being investigated, both as adjuvant to the local therapeutic modalities, as well as therapeutically for unresectable metastases. While the first two systemic modalities have established themselves in cancer management, immunotherapy has been lagging behind for several reasons.     

A seroprevalence study of poliovirus antibody in the population of northern Greece

This study assessed immunity to poliomyelitis in a representative sample of 1064 persons living in northern Greece. Antibody prevalences in the individuals tested were 91.1% (95% confidence interval (CI): 89.4-92.8), 92.1% (95% CI: 90.5-93.7) and 83.1% (95% CI: 80.8-85.4) for poliovirus types 1, 2 and 3, respectively. For poliovirus type 3, a gap in immunity was found in individuals aged 10-29 years. Re-vaccination of adolescents living in northern Greece is suggested to ensure herd immunity and to minimise the risk of importation of wild poliovirus from endemic countries.     

豆根管食通口服液治疗食管癌的临床研究

目的 :观察豆根管食通口服液治疗食管癌的临床疗效及对其免疫功能的影响 ,并探讨其分子作用机制。方法 :采用随机、对照的方法 ,把符合标准的 6 0例食管癌患者分为对照组和治疗组各 30例 ,分别给予“豆根管食通口服液”和“替加氟片”治疗 ,重点观察治疗前后患者的一般状况、症状体征、生活质量、心电图、超声波、食管钡餐X片、血常规、肝肾功能、白介素 (IL -2、IL - 12 )、干扰素 (IFN -γ)、血清免疫球蛋白 (IgA、IgG、IgM)等的变化情况。结果 :治疗前后两组比较 ,其一般状况、症状体征、生活质量、临床疗效经各评分评定均有显著性差异 (P <0 .0 1) ,食管钡餐X片治疗前后达Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级标准比较有显著性差异 (P <0 .0 5 ) ,细胞因子比较有显著性差异 (P <0 .0 1,P <0 .0 5 )。结论 :豆根管食通口服液能明显改善食管癌患者的一般状况和症状体征、提高患者的生活质量、减轻患者的临床症状 ,能明显提高食管癌患者的血清IL - 2、IL - 12、IFN -γ及IgA、IgG、IgM的水平 ,提高机体的免疫功能等。