Hepcidin response to acute iron intake and chronic iron loading in dysmetabolic iron overload syndrome

Background: The pathogenesis of dysmetabolic iron overload syndrome (DIOS) is still unclear. Hepcidin is the key regulator of iron homeostasis controlling iron absorption and macrophage release. Aim: To investigate hepcidin regulation by iron in DIOS. Methods: We analysed urinary hepcidin at baseline and 24 h after a 65 mg oral iron dose in 24 patients at diagnosis and after iron depletion (n=13) and compared data with those previously observed in 23 healthy controls. Serum iron indices, liver histology and metabolic data were available for all patients. Results: At diagnosis, hepcidin values were significantly higher than in controls (P<0.001). After iron depletion, hepcidin levels decreased to normal values in all patients. At baseline, a significant response of hepcidin to iron challenge was observed only in the subgroup with lower basal hepcidin concentration (P=0.007). In iron‐depleted patients, urinary hepcidin significantly increased after oral iron test (P=0.006). Conclusions: Ours findings suggest that in DIOS, the progression of iron accumulation is counteracted by the increase in hepcidin production and progressive reduction of iron absorption, explaining why these patients develop a mild–moderate iron overload that tends to a plateau.     

当归多糖对H22荷瘤小鼠肿瘤生长及体内铁代谢的影响

目的:研究当归多糖(Angelica sinensis polysaccharide,ASP)对H22荷瘤小鼠肿瘤生长及体内hepcidin和IL-6表达的抑制作用。方法:移植H22鼠肝癌细胞于小鼠右腋皮下,建立H22荷瘤小鼠模型,分为空白组(不接种不给药),阴性对照组(腹腔注射等体积生理盐水),阳性对照组(腹腔注射环磷酰胺,25 mg·kg^-1),ASP高剂量组(灌胃ASP溶液,100 mg·kg^-1),ASP中剂量组(灌胃ASP溶液,50 mg·kg^-1),ASP低剂量组(灌胃ASP溶液,25 mg·kg^-1),连续给药14 d,末次给药24 h后摘眼球取血,剥离肿瘤、脾脏并称重,采用ELISA法检测血清中hepcidin和IL-6的含量变化。结果:3个剂量组当归多糖均有一定的抗肿瘤效果,抑瘤率分别为24.12%,29.15%和34.67%,同时能显著抑制hepcidin和IL-6的表达。结论:当归多糖的体内抗肿瘤作用可能与其对铁代谢的调节有关。     

大强度运动后低氧对大鼠炎症反应和十二指肠铁吸收蛋白的影响

目的:研究低氧对大鼠炎症反应和肠铁吸收相关蛋白的影响,阐明低氧影响机体铁状态的调节机制。方法:24只雄性Wistar大鼠随机分为安静对照组(C组)、大强度运动组(E组)和运动后低氧恢复组(HE组)。E组和HE组进行递增负荷跑台运动,坡度0,速度30 m/min,6 d/周,前2周1次/天,后2周2次/天,共4周。训练第1次为1 min,每次递增2 min。HE组每天运动后在13.6%O2的低氧舱中休息,8 h/d。检测各组血清铁,ELISA法检测血清铁调素(hepcidin)和白介素6(IL-6),Western Blot法检测十二指肠铁转运蛋白:血红素转运体1(Heme transport protein1,HCP1)、二价金属离子转运蛋白1(divalent metal transporter 1,DMT1)和膜铁转运蛋白1(Ferroportin 1,FPN1)表达。结果:(1)HE组血清铁和Hb显著高于C组和E组(P<0.01,P<0.05),血清hepcidin、IL-6、白细胞数显著低于E组(P<0.05);而E组血清铁和Hb显著低于C组(P<0.05,P<0.01)。(2)HE组HCP1、DMT1、FPN1均显着高于C组和E组(P<0.01,P<0.05)。结论:适度低氧降低运动机体炎症反应,减少肝分泌hepcidin,促进肠铁吸收,改善机体铁状态,预防运动性铁缺乏。     

铁调素的表达与调节机制研究进展

铁调素调节细胞铁的外向转运,其表达受机体信号因子(如血清铁和促红细胞生成素水平)以及炎症、低氧等疾病状态的影响。这些刺激通过肝实质细胞表面的组织相容性Ⅰ型跨膜糖蛋白HFE,转铁蛋白受体1和2,铁调素调节蛋白激活BMP-SMAD、JAK-STAT和HIF1等信号通路,改变铁调素基因的转录,调节铁调素的表达水平。但目前对调节铁调素表达的分子机制还不明确。本文从影响铁调素表达的信号因子及参与其表达调控的信号通路两方面入手,对铁调素表达的调节机制方面的最新研究进展加以综述。     

Does the Combined Effect of Resistance Training with EPO and Iron Sulfate Improve Iron Metabolism in Older Individuals with End-Stage Renal Disease?

We sought to investigate the effects of resistance training (RT) combined with erythropoietin (EPO) and iron sulfate on the hemoglobin, hepcidin, ferritin, iron status, and inflammatory profile in older individuals with end-stage renal disease (ESRD). ESRD patients (n: 157; age: 66.8 ± 3.6; body mass: 73 ± 15; body mass index: 27 ± 3), were assigned to control (CTL; n: 76) and exercise groups (RT; n: 81). The CTL group was divided according to the iron treatment received: without iron treatment (CTL—none; n = 19), treated only with iron sulfate or EPO (CTL—EPO or IRON; n = 19), and treated with both iron sulfate and EPO (CTL—EPO + IRON; n = 76). The RT group followed the same pattern: (RT—none; n = 20), (RT—EPO or IRON; n = 18), and (RT—EPO + IRON; n = 86). RT consisted of 24 weeks/3 days per week at moderate intensity of full-body resistance exercises prior to the hemodialysis section. The RT group, regardless of the iron treatment, improved iron metabolism in older individuals with ESRD. These results provide some clues on the effects of RT and its combination with EPO and iron sulfate in this population, highlighting RT as an important coadjutant in ESRD-iron deficiency.     

急性淋巴细胞白血病患儿血清铁调素水平及临床意义

目的 探索急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)患儿血清铁调素(Hepcidin)水平及其临床意义.方法 选择ALL患儿30例为研究对象,随访时间截止到2020年2月,应用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检...     

Fruit and Vegetable Consumption and Their Association With the Indicators of Iron and Inflammation Status Among Adolescent Girls

Background: The objective of this study was to identify an association among dietary components, iron, and inflammatory status among adolescent girls.

Method: Dietary information for 85 adolescent girls was collected through food frequency questionnaires. Biomarkers of iron and inflammatory status were analyzed.

Results: We found that 28.2% of adolescent girls had anemia and 65.9% girls were iron-deficient. Girls who did not consume guava had 3.8-fold (95% confidence interval =1.1–9.4; p?=?0.020) increased the risk of having low serum iron levels. Girls who consumed amaranth had significantly (p?=?0.024) higher serum hepcidin levels (n?=?44; 129.7?±?81.40?pg/mL vs n?=?41; 94.6?±?55.8?pg/mL) as well as ferritin levels (n?=?44; 19.7?±?16.4?µg/L vs n?=?41; 14.0?±?10.2?µg/L). Overall consumption of fruits and green leafy vegetables among girls significantly affects their iron status.

Conclusions: Regular consumption of vitamin C–rich fruits and green leafy vegetable intake are imperative for improvement of iron status among adolescent girls.