凋亡相关基因突变与人类疾病

细胞凋亡 (又称细胞程序性死亡 )过程由多种不同基因产物的协同作用来完成。其中某些凋亡基因的突变 ,已被认定为人类疾病的病因或相关因素。作者综述了当前对凋亡基因突变所致人类疾病的认识 ,以及将凋亡的基础研究成果应用到临床疾病诊治的情况     

温州市外来流动人口传染病的监测

目的：为了解温州市流动人口传染病的发病情况，加强这些人群的传染病防制和监测工作。方法：我们对温州市1997－2000年甲，乙类传染病报告资料进行调查，整理和分析。结果：（1）流动人口传染病年均报告发病率为44．75／10万；（2）省外流动人口传染病报告发病率（55．55／10万）高于省内（12．03／10万）；（3）流动人口传染病发病率以呼吸道，肠道，血源和性传播疾病为高，而虫媒与自然疫源性疾病和新生儿破伤风相对较低；（4）流动人口中肺结核，乙肝，甲肝和痢疾的发病率较高，其发病数均占总病数的10％以上；（5）流动人口疟疾和新生儿破伤风发病率高于当地人群，而其它传染病发病率则低于当地人群。结论：目前，流动人口传染病问题已日益突出，对社会稳定和经济发展将发生严重影响，采取合适的防制措施对控制流动人口传染病是非常迫切和必要的。     

Periodontal conditions among the middle-aged and the elderly in Hong Kong

Abstract The aim of this study was to describe the periodontal conditions in 372 35–44-yr-old and 537 noninstitutionalized 65–74-yr-old Hong Kong Chinese who were examined clinically for loss of attachment, recession, probing depth, calculus, and bleeding after probing. Community Periodontal Index (CPI) data and treatment need indications were compiled from index teeth or their substitutes. The prevalence of loss of attachment varied considerably in both cohorts according to the definition of the threshold (≥6, ≥9, and ≥12 mm, respectively). The mean numbers of teeth with loss of attachment at the ≥6-mm threshold and at higher thresholds were small. In both age cohorts, about one-fifth of subjects had probing depths ≥6-mm, while al the ≥9-mm threshold only 2–3% were so affected. Although recession was an important component of loss of attachment in the younger cohort, in the older cohort the prevalence and extent of recession were greater than for probing depths at thresholds ≥4 mm. All subjects had one or more teeth with calculus, bleeding, or both, most teeth being so affected. Eighty-four of the 537 65–74-yr-old subjects were excluded either because of edentulousness or because extractions indicated for the remaining teeth would have rendered the subjects edentulous. The distribution of subjects according to their highest CPI score was remarkably similar for the two cohorts. No subjects in either age group were assessed as “healthy” (CPI code 0) or had “bleeding only” (code 1) as their highest score. While most subjects scored CPI code 2 or 3 us their highest score, only 17% of the younger and 15% of the older cohort scored Community Periodontal Index of Treatment Needs (CPITN) code 4. Differences in the mean number of sextants affected by CPI codes between the two cohorts were mainly due to a greater number of excluded sextants in the older cohort. CPI findings for 35–44-yr-olds differed little from those reported in 1984.     

浅谈对传染科护士素质的要求与体会

根据笔者在传染科病房工作中的体会,从六个方面针对传染科工作的特殊性和存在的共性分别阐述了传染科护士在工作中如何高标准、严要求自己,如何对病人做到到位的护理,旨在提高传染科病房的整体护理质量,从而把更多的实惠让利于患者。     

Comparative effects of plateletderived growth factor-BB and insulin-like growth factor-I, individually and in combination, on periodontal regeneration in Macaca fascicularis

Platelet-derived growth factor (PDGF) and insulin-like growth factor I (IGF-I) in combination have previously been shown to enhance periodontal regeneration. The objective of this study was to further characterize the biological effects of this combination of growth factors in non-human primates and compare the effects to those of each growth factor individually. Ligature-induced periodontitis was initiated in 10 cynomolgus monkeys. After periodontal lesions were established, surgery was performed, and either a methylcellulose gel vehicle or vehicle containing 10 μg each of either PDGF-BB, IGF-I or both PDGF-BB and IGF-I was applied to exposed root surfaces. Biopsies were taken 4 and 12 wk after treatment and the extent of periodontal regeneration was assessed by histomorphometry. At both 4 and 12 wk vehicle-treated lesions generally revealed minimal osseous defect fill (ODF) (8.5±2.1% and 14.5±5.7%, respectively) and new attachment (NA) (34.1±5.2% and 26.6±10.5%, respectively). IGF-I treatment did not significantly alter healing compared to vehicle in any parameter at both 4 and 12 wk. PDGFBB-treated sites exhibited significant (p<0.05) regeneration of NA (69.6±12.0%) at 12 wk; trends for PDGF-BB treatment effect were also observed in other parameters at 4 and 12 wk. although these increases were not statistically significant. Treatment with PDGF-BB/IGF-I resulted in 21.6±5.1 % and 42.5±8.3% ODF at 4 and 12 wk, respectively, and 64.1±7.7% and 74.6±7.4% NA at 4 and 12 wk, respectively (all significantly greater than vehicle, p<0.05). The results from this study demonstrated that: 1) IGF-1 alone at the dose tested did not significantly alter periodontal wound healing; 2) PDGF-BB alone significantly stimulated NA, with trends of effect on other parameters; and 3) the PDGF-BB/IGF-I combination resulted in significant increases in NA and ODF above vehicle at both 4 and 12 wk.     

2002年广东省H3N2亚型流感病毒流行的分子基础

目的 了解广东省2002年H3N2亚型流感病毒流行及抗原性变异情况。方法 用狗肾传代细胞(MDCK)和鸡胚进行流感病毒分离，用交叉血凝抑制实验对毒株进行抗原性分析；提取病毒RNA，用一步法RT-PCR扩增血凝素基因(H3A)，产物纯化后，并将其克隆到pMD18—T Vector，进行序列测定和分析。结果 2002年1-10月共获得流感病毒246株，其中H3N2亚型210株，占85．4％，H1N1亚型2株，占0．8％，B型34株，占13．8％；2002年流感流行的高峰是6月；抗原性分析显示，2002年广东省H3N2亚型流感病毒A／粤/236/2002和2001年流行株A／粤／448／2001以及目前国内代表株A／闽／151／2001的抗原比分别是5．6和4．0；其HAl区氨基酸序列和国际代表株A／悉尼／5／97同源性为94．2％，有19个氨基酸差异，其中发生在抗原决定族A、B、E上的有6个，受体结合部有3个。结论 2002年广东省流感病毒以H3N2亚型为主，其流行的分子基础是基因变异导致抗原性发生漂移。     

天然抗氧化剂多靶点作用在防治心脑血管疾病中的研究进展

从天然抗氧化剂的多靶点作用角度阐述其防治心脑血管疾病的效应机制、研究动态，并提出存在的问题及解决思路。     

碘过量与甲状腺疾病

介绍近年来国内外对高碘致甲状腺肿、甲状腺功能亢进症、甲状腺功能减退症、自身免疫甲状腺炎、甲状腺癌的研究进展,阐述防治碘缺乏病(IDD)应当因地因人分类设计,盲目补碘和过度补碘都是错误的。     

经皮椎体成形术治疗脊柱疾病

目的评价经皮椎体成形术治疗脊柱疾病的临床应用价值.方法 56例多发骨髓瘤、溶骨性脊柱转移瘤、骨质疏松性椎体压缩性骨折患者应用经皮椎体成形术后,分24 h、3个月两阶段评估患者疼痛、术后X线片检查结果、椎体高度等指标.结果术后止痛效果良好,尤以骨质疏松性椎体压缩性骨折患者的止痛效果最好.无严重并发症. 结论经皮椎体成形术对骨质疏松性椎体压缩性骨折等脊柱疾病的止痛、稳固椎体等效果明显,可以谨慎开展.