重肝注射液对大鼠急性黄疸的疗效研究

重肝注射液是用传统治疗黄疸性肝炎的中草药提取物配伍所制成的复方注射剂,本研究用异硫氰酸-α-萘酯(ANIT)造成的大鼠急性黄疸性肝损害模型,证实静脉滴注该制剂对急性黄疸性肝损害有良好的治疗作用,能降低血清胆红素及谷丙转氨酶,减轻肝细胞的损伤程度,为该制剂的临床使用提供了科学理论依据。     

三种肝炎模型的生化指标与病理形态学的对比研究

采用α-萘异硫氰酸酯(ANIT)、四氯化碳(CCl_4)和半乳糖胺(Glan)复制肝炎模型,进行血清生化指标和肝组织学的对比研究。结果证明,ANIT 复制肝炎模型能明显升高血清胆红素和谷丙转氨酶,肝细胞和胆管亦见明显损害。可用于筛选退黄和降酶药物的研究。CCl_4复制肝炎模型则以升高SGPT 为主,组织学仅见明显的肝细胞损害,可用于筛选降酶药物的研究。Glan 复制肝炎模型,生化指标和肝组织学改变均不明显,认为该模型不甚理想。     

新疆维吾尔族、汉族冠心病患者血清胆红素水平与冠状动脉病变积分的相关性研究

目的:研究新疆维吾尔族(维族)、汉族冠心病患者血清胆红素水平与冠状动脉病变积分(CSS)的相关性研究.方法:分别将242例维族和238例汉族患者按冠状动脉造影结果分为冠心病组和对照组,采用Gensini法计算CSS.检测窄腹血清总胆红素、直接胆红素、间接胆红素、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白水平.结果:不同性别不同年龄段的血清胆红素水平在维、汉两个民族之间差异无统计学意义;维、汉两个民族冠心病组血清总胆红素、直接胆红素、间接胆红素水平明显低于其对照组(P<0.05),且随着CSS增高,总胆红素水平也降低(P<0.05),差异有统计学意义;多因素Logistic回归分析显示随着总胆红素水半的降低,冠心病的发病风险增大(P＝0.000);Pearson直线相关分析显示维族及汉族冠心病患者的血清胆红素水平与其CSS呈负相关(P<0.05).结论:维族、汉族患者的血清胆红素水平与其是否患冠心病的危险及CSS呈显著负相关.维、汉两个民族的血清胆红素水平无民族差异.     

冠状动脉病变程度与血清总胆红素和尿酸的相关性分析

目的探讨冠状动脉病变程度与血清总胆红素和尿酸的关系。方法选择78例曾进行选择性冠脉造影的病人,按冠脉狭窄程度分为4组,进行血清总胆红素和尿酸测定。结果血清总胆红素与冠脉狭窄的严重程度呈独立的负相关,而血清尿酸与冠脉狭窄的严重程度呈独立的正相关。结论血清总胆红素、尿酸含量作为反映冠心病患者体内氧化与抗氧化系统活动情况的生化指标,对预测冠脉病变程度的进展及预后有指导意义。     

Enzymatic determination of bilirubin fractions in serum

Novel enzymatic methods using bilirubin oxidase from Myrothecium verrucaria are described for the determination of bilirubin fractions in serum. These fractions include an unconjugated form (Bu), a conjugated form (Bc), and a delta fraction of bilirubin that reacts with direct diazo reagent and is irreversibly bound to serum albumin (Bδ). The determination is based on the different reactivities of the enzyme to bilirubin fractions at different pH in the presence or absence of anionic detergent such as sodium dodecyl sulfate (SDS) or sodium cholate. Thus, in the absence of detergents, Bc and Bδ are oxidized at acidic pH, while Bc is oxidized at alkaline pH; Bu is not oxidized at either acidic or alkaline pH.

The first approach is based on measuring the decreased absorbance of bilirubin colour at 450 nm caused by the enzymatic oxidation. Total bilirubin is measured at pH 8.0 in the presence of SDS. Direct bilirubin is measured at pH 3.7 and Bc is measured at pH 10.0 in the absence of SDS, respectively.

The second approach is made by coupling the diazo reaction with the enzyme reaction. Total and direct bilirubin are determined by a conventional diazo method without prior treatment by the enzyme. Bδ is determined with a direct diazo method after the serum sample is treated at pH 10.0 by the enzyme to oxidize the Bc fraction. The precision and the accuracy of these methods were compared with a precision and the accuracy of these methods were compared with a diazo method, an HPLC method and a slice method, and good results were obtained.     

Action mechanism of Yi Guan Jian Decoction on CCl4 induced cirrhosis in rats

Aim of study

To investigate action mechanism of Yi Guan Jian Decoction on cirrhosis induced by CCl₄ in rats.

Material and methods

CCl₄ (3 mL/kg) for the first time and then olive oil CCl₄ solution 50% (2 mL/kg) was administered hypodermically to rats twice each week for 12 weeks. At the end of 8th week, rats were randomly divided into CCl₄ control group (n = 10), Yi Guan Jian Decoction group (n = 9) and Xiao Chai Hu Decoction group (n = 9). Yi Guan Jian Decoction and Xiao Chai Hu Decoction were oral administrated per day respectively for 4 weeks, concomitantly continued CCl₄ administration. At 12th weekend, the rats were sacrificed for sampling and detection of liver function, histological changes of liver tissue, liver tissue hydroxyproline content and expression of α-SMA, CD68, MMP-13, TIMP-1, TIMP-2, Caspase-12, HGFα, MMP-2, MMP-9 and hepatocyte apoptotic index.

Results and conclusions

(1) Compared with that of normal rats, expression of α-SMA, CD68 and TIMP-1 in liver tissue of 8 week model group rats increases significantly (P < 0.01), moreover further increased in the 12 week of model group. However, MMP-13, HGFα, TIMP-2 content decreases gradually and the statistical difference is seen between each time point (P < 0.01). Activity of MMP-2, MMP-9, content of Caspase-12 and hepatocyte apoptotic index increased gradually at 4th, 8th, 12th week. (2) Compared to that of the same time point model group, activity of MMP-9 and contents of MMP-13, TIMP-2 and HGFα in Yi Guan Jian Decoction group improves significantly (P < 0.01), and activity of MMP-2 and contents of α-SMA, TIMP-1, Caspase-12 and hepatocyte apoptotic index decreases significantly (P < 0.01). This work suggests that Yi Guan Jian Decoction exerts significant therapeutic effect on CCl₄-induced cirrhosis in rats, through mechanism of inhibiting hepatocytes apoptosis and hepatic stellate cells activation, and regulating the function of Kupffer cell.

Ethnopharmacological relevance

This study investigates the mechanism of Yi Guan Jian against cirrhosis from aspect of heptocytes apoptosis and hepatic stellate cells activation. It suggest that although of unknown bioactive ingredients, mechanism of traditional Chinese medicine recipe against cirrhosis can be disclosed and of profound significance.     

认知矫正联合常规药物治疗双相情感障碍患者疗效及对TBIL、UA和BDNF的影响

目的研究认知矫正联合常规药物治疗双相情感障碍患者疗效及对脑源性神经营养因子(BDNF)、总胆红素(TBIL)、尿酸(UA)水平的影响。 方法选取106例双相情感障碍患者分为常规组(n＝53)和联合组(n＝53)。常规组给予常规药物治疗,联合组给予认知矫正联合常规药物治疗。比较两组治疗总有效率、TBIL、UA、BDNF、认知评价、临床疾病严重度量表-双相情感障碍版(CGI-BP)评分、汉密尔顿抑郁量表(HAMD)评分及躁狂量表(BRMS)评分。 结果联合组治疗总有效率高于常规组(P＜0.05)。与治疗前比较,治疗后两组BDNF、TBI水平均升高,UA、CGI-BP评分、HAMD评分及BRMS评分均降低,且联合组改善较常规组更为显著(P＜0.05)；治疗后,联合组视觉再生图像b、图像c、视觉再生总分、威斯康星卡片分类测试(WCST)分数类、数字符号得分均升高,连线测试A、连线测试B、WCST错误数、持续错误数等得分均降低,且联合组治疗后较常规组治疗后改善更为显著(P＜0.05)。 结论认知矫正联合常规药物治疗双相情感障碍可提升治疗效果,改善临床症状,提高神经认知功能,具有较高临床价值。 [     

血清胆红素与冠心病冠状动脉狭窄病变程度及血脂的关系

【目的】探讨血清胆红素与冠心病冠状动脉狭窄病变程度及血脂的关系。【方法】将112例已行冠状动脉造影患者分为冠心病组及对照组（冠状动脉造影正常者），比较两组间血清总胆红素（TBIL）、直接胆红素（DBIL）、间接胆红素（IBIL）及血脂水平，并对胆红素与血脂进行直线相关分析。【结果】冠心病组血清TBIL、DBIL和IBIL水平均低于对照组（P〈0．05），总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）均高于对照组（P〈0．05）。冠心病组中冠状动脉多支病变者血清TBIL、DBIL和IBIL水平均低于单支病变者（P〈0．05）。相关分析显示，血清胆红素与TC、TG和LDL-C呈负相关。【结论】血清胆红素与血脂相关，可能参与血脂代谢。低胆红素血症易患冠心病，血清胆红素水平越低，冠状动脉狭窄病变程度越重。     

急性冠脉综合征与血清胆红素水平的相关性分析

目的探讨急性冠脉综合征与血清胆红素水平的相关性.方法采集空腹静脉血检测76例急性冠脉综合征患者及38例对照组的血清胆红素和血脂水平.将急性冠脉综合征患者分为不稳定型心绞痛组和急性心肌梗死组,与对照组比较血清胆红素水平的差异,并进行血清胆红素与血脂Pearson直线相关分析.结果不稳定型心绞痛组和急性心肌梗死组血清总胆红素和直接胆红素水平均明显低于对照组,差异有统计学意义(P＜0.01).不稳定型心绞痛组和急性心肌梗死组之间血清总胆红素和直接胆红素水平差异无统计学意义(P＞0.05).血清总胆红素和直接胆红素与总胆固醇、三酰甘油、低密度脂蛋白均呈负相关(P＜0.05);与高密度脂蛋白呈正相关(P＜0.05).结论血清胆红素水平降低可能是急性冠脉综合征发生和发展的危险因素.     

In vitro protein binding of teicoplanin to neonatal serum

The protein-binding ability of teicoplanin (TEIC) to neonatal serum was investigated and the following results were obtained. (1) The protein binding ability of TEIC at concentrations ranging from 10 to 100µg/ml, to neonatal serum was 80.5%–71.9% and was generally low as compared with that for adult serum. (2) The protein binding ability of TEIC (20µg/ml) to albumin in neonatal serum was not concentration-dependent, and the correlation between the protein-binding ability and albumin concentration was low. Namely, it was suggested that the protein-binding ability of TEIC to neonatal serum was not dependent merely on the albumin concentration, and there was a possibility that the protein-binding ability also depended on qualitative changes in albumin. (3) The protein-binding ability of TEIC (20µg/ml) to total bilirubin and free bilirubin was not concentration-dependent, and the correlation was extremely low, indicating that there is a low possibility that the protein-binding ability of TEIC induces an increase in free bilirubin.