Neovascularization profile in rat allogenic penetrating keratoplasty

AIM: To observe the neovascularization process with no intervention in rat allogenic penetrating keratoplasty. METHODS: Allogenic penetrating keratoplasties were successfully performed in 34 female SPF SD rats with no intervention after operations. Corneal neovascularization (CNV) process was noted on day 4, 7, 15 and 30 with operating microscope. The vascular area surface was calculated using the formula C /12×3.14×[r ²-(r -I )²]. RESULTS: CNV was noted in 29 out of 34 rats (85%). Firstly, the new vessels distributed around the cornea like a brush then gradually extended towards the center. The vessels were distorted and massive with branched tails, they continued growing to reticulated veins in peak time then gradually atrophied. The average neovascularization area (SE) on day 4, 7, 15 and 30 was 11.8±3.5mm², 18.5±4.0mm²,14.4±4.3mm² and 6.0±1.8mm², respectively and 12.7±1.9mm² in total. The average percentage that new vessels accounting the whole cornea area (SE) was 30.8%±8.7%, 65.3%±12.8%, 59.4%±14.5%,36.2%±10.9% and 48.7%±6.4% in total. CONCLUSION: In rat allogenic penetrating keratoplasties without intervention, CNV presents on day 4 and reaches the maximum area on day 7. Then the vessels gradually atrophies, about 50% of the maximum area still remains on day 30.     

联合片状同种异体结膜移植与羊膜移植治疗早期碱烧伤

目的：探讨联合羊膜的小片状同种异体结膜移植重建眼表的可行性。 方法：新西兰白兔双眼上方球结膜碱烧伤后24h,实验组行以羊膜为基底的小片状同种异体结膜移植,对照组行单纯小片状同种异体结膜移植,术后各周行印迹细胞的PAS染色、扫描电镜检查。 结果：实验组未见明显排斥反应,全部植片成活,对照组7眼植片排斥脱落,植片成活率81．6％,明显低干实验组（P〈0．05）。实验组术后4wk,对照组术后第5wk结膜外观、杯状细胞形态、数目、功能均恢复正常。 结论：羊膜与小片状同种异体结膜移植相结合可能成为修补早期大面积结膜碱烧伤的有效途径。     

The role of allogeneic hematopoietic stem cell transplantation and Epstein‐Barr virus infection on the treatment for child primary hemophagocytic lymphohistiocytosis patients with X‐linked lymphoproliferative disease: A rare case report and family survey study

XLP‐2 is known as a rare primary immunodeficiency disease, which is characterized by the susceptibility to EBV infection and potential development into the pHLH. The existing studies believe that the dysfunction of XIAP represents one of the most significant pathogenic mechanisms of XLP‐2, and allo‐HSCT is regarded as a crucial treatment for the long‐term survival in XLP‐2 patients. In our present study, a 2‐year‐old male patient was diagnosed with XLP‐2. After receiving chemotherapy by using HLH‐2004 without allo‐HSCT, he reached a complete remission, and his EBV load was brought under control. Our family survey revealed a novel frameshift mutation in the XIAP gene in this patient, as well as in his cousin and grandfather. Until now, the patient has been followed up for 22 months with no recurrence reported yet. Based on these findings, it is believed that for child pHLH patients with XLP‐2, the treatment by controlling symptoms alone without allo‐HSCT and with regular monitoring of EBV load could be conducive to a long‐term survival.     

异体脱细胞真皮基质修复口腔黏膜缺损的临床应用

目的：评价异体脱细胞真皮基质修复口腔黏膜缺损的临床应用。方法：选择9例因口腔肿瘤和癌前病变而导致黏膜缺损病例,以异体脱细胞真皮基质修复口腔黏膜缺损,对修复后的创面愈合情况进行观察。结果：8例以异体脱细胞真皮基质修复的缺损创面完全愈合,1例移植的异体脱细胞真皮基质约10％脱落,缺损创面正常愈合,4周后异体脱细胞真皮基质表面完全上皮化,与周围正常黏膜无明显分界。结论：异体脱细胞真皮基质可作为修复口腔黏膜缺损的一种选择。     

尸体肾移植13例次的回顾与分析

１９９２年１２月～１９９６年４月的五年间共完成同种异体肾脏移植１３例次，从本组临床资料的回顾性分析显示：①高质量的取肾与植肾手术是预防急性肾功能衰竭，提高存活率的关键，②严格选择供、受者和充分术前准备是减少术后并发症，提高肾移植近期疗效的保障     

股动脉—胫后血管旁路术治疗血栓闭塞性脉管炎(附4例报告)

本文报道了采用股动脉—胫后血管旁路水治疗4例Ⅲ期血栓闭塞性脉管炎患者。其中尤其是股动脉—胫后静脉通路的建立，兼有静脉动脉化的特点，能迅速改善肢体缺血。同时在本组的血管旁路术中，有2例采用经处理后的异体静脉，屯获得了满意的近期疗效。     

Titanium implant insertion into dog alveolar ridges augmented by allogenic material

The purpose of this investigation was to evaluate whether titanium endosseous implants would osseointegrate in dog alveolar ridges augmented by allogenic material. In 8 dogs en bloc resection, including 2 premolars, was performed bilaterally in the maxilla and the mandible. After a healing period of 6 weeks allogenic, demineralized and lyophilized dentin or bone was implanted subperiosteally. Titanium implants were installed 5.5 months later in some of the regions. Light and fluorescence microscopic evaluation revealed fibrous encapsulation of the implanted allogenic material, no osteoinduction and only minimal osteoconduction. few multi‐nuclear giant cells and a sparse inflammatory reaction. The titanium implants healed mainly by fibrous encapsulation.