张艳教授治疗慢性心力衰竭经验

张艳教授认为,慢性心力衰竭属本虚标实之证,本虚以心气虚衰、肾气亏损为主,标实以瘀血内阻为重,临床往往虚实夹杂,虚实俱重.在治疗上独出己见,提出"心肾同治,气血同调"的治则.心肾同治以补益心肾为重,气血同调以益气活血为佳.用补法扶正以调和脏腑气血、阴阳的平衡,用益气活血法缓标证之急,寓通于补,补而不滞,气血畅通.     

Bioequivalence Assessment of Topical Ophthalmic Drugs Using Sparse Sampling Pharmacokinetics Data

<正>In the development of eye drop medications,difficulty in sampling is a major challenge[1]. Drug concentrations in the aqueous humor (AH) can only be measured when the eye is undergoing surgery,such as cataract replacement. Sampling from tears may significantly reduce the amount of medication remaining in the eye. Owing to limitations caused by sampling difficulty, the concentration–time profile     

Anti-obesity Action of Fermented Barley Extracts with Lactobacillus plantarum dy-1 and Associated Micro RNA Expression in High-fat Diet-induced Obese Rats

Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese rats.Methods Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the test.Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.Conclusion These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.     

Comparison of Xpert MTB/RIF,Real Amp,and CPA Tests in Detecting Mycobacterium tuberculosis

Today, tuberculosis (TB) remains a global public health threat associated with significantly high rates of morbidity and mortality. The World Health Organization's (WHO) Global Tuberculosis Report 2018[1] has reported that in 2017, 10.0 million people across the world had developed TB diseases that resulted in an estimated 1.6 million deaths, and 889, 000 people developed TB in China that led to 39, 000 TB-related deaths. Therefore, rapid and accurate detection of Mycobacterium tuberculosis (MTB) is important for initiating early treatment and reducing mortality. Traditional diagnostic methods for pulmonary TB incorporate chest radiography and sputum smear microscopy;however, several cases of tuberculosis go undiagnosed because of the low sensitivity of smear microscopy[2].     

维吾尔医药学专著《阿日普验方》

《阿日普验方》为《木接热巴提阿日普》（Mujarribati Arip）的意译名，是由维吾尔医学家毛拉阿日普忽炭尼（Molla Arip Hotani），约于1620年以波斯文撰写的一部维吾尔医药学专著，现有2部察合台维吾尔文抄本，分别藏于新疆维吾尔医学专科学校和一位维吾尔医师家中。该书介绍维吾尔医学四大物质论、气质论、体液论、诊断知识、治疗法（食疗、药疗）及一些常用草药、成药，是一部具有经典著作地位和重要影响的维吾尔医药学专著，应当整理、出版，以广传播。     

抗炎消凝通抗炎的实验研究

目的 研究以化痰散结，活血化瘀为组方原则的“抗炎消凝通”的抗炎作用。方法 通过4种不同动物模型观察给药后的抗炎作用。结果 抗炎消凝通具有以下作用；（1）高、低浓度组均能显地对抗二甲苯所致的炎症；（2）中、高浓度组能显地消除由角叉菜胶引起的肿胀；（3）中、高浓度组均有显地抗肉芽肿作用，并优于强的松；（4）对流感病毒感染小鼠所致肺部病变有显抑制作用。结论 抗炎消凝通具有显抗炎作用，以中浓度效果最佳。     

Amplitude screening improves performance of AMSA method for predicting success of defibrillation in swine model

PurposeA novel amplitude screening method, termed Optimal Amplitude Spectrum Area (Opt-AMSA) with the aim of improving the performance of the Amplitude Spectrum Area (AMSA) method, was proposed to optimize the timing of defibrillation. We investigated the effects of the Opt-AMSA method on the prediction of successful defibrillation when compared with AMSA in a porcine model of ventricular fibrillation (VF).Method60 male domestic pigs were untreated in the first 10 min of VF, then received cardiopulmonary resuscitation (CPR) for 6 min. Values of Opt-AMSA and AMSA were calculated every minute before defibrillation. Linear regression was used to evaluate the correlation between Opt-AMSA and AMSA. Receiver Operating Characteristic (ROC) analysis was conducted for the two methods and to compare their predictive values.ResultsThe values of both AMSA and Opt-AMSA gradually decreased over time during untreated VF in all animals. The values of both methods of defibrillation were slightly increased after the implementation of CPR in animals that were successfully resuscitated, while there were no significant changes in either method in those who ultimately failed to resuscitate. The significant positive correlation between Opt-AMSA and AMSA was shown by Pearson correlation analysis. ROC analysis showed that Opt-AMSA (AUC = 0.87) significantly improved the performance of AMSA (AUC = 0.77) to predict successful defibrillation (Z = 2.27, P < 0.05).ConclusionBoth the Opt-AMSA and AMSA methods showed high potential to predict the success of defibrillation. Moreover, the Opt-AMSA method improved the performance of the AMSA method, and may be a promising tool to optimize the timing of defibrillation.     

糖尿病肾病肾间质纤维化“肾络癥瘕聚散消长”病机内涵

糖尿病肾病肾间质纤维化的主要病理特点为胞外基质降解异常,在肾小球系膜和肾小管间质进行性沉积,这与其"肾络癥瘕聚散消长"的核心病机存在相似之处。转化生长因子(transforming growth factor-bata, TGF-β)/Smad作为致肾间质纤维化的重要通路之一,其平衡失调受UPS和自噬的双重影响,这与中医"肾络癥瘕聚散消长"理论中,"邪聚正散"的失衡状态相一致。前期已证实,和解聚散法可以抑制TGF-β/Smad通路缓解糖尿病肾病肾间质纤维化,其作用机制可能与调节糖尿病肾病病状态下自噬与泛素蛋白降解途径的平衡有关,但仍需加强实验验证明确和解聚散法干预TGF-β/Smad信号通路是否与抑制泛素蛋白酶体降解通路有关,是否与激活自噬有关,进一步研究和解聚散法干预自噬-溶酶体降解途径的具体节点,同时加强体外实验,阐明其含药血清是否通过干预上述通路发挥作用,明确干预上述通路可增加TGF-β/Smad通路中哪些关键蛋白的降解。今后需进一步研究和解聚散法调节糖尿病肾病病状态下自噬与泛素蛋白降解途径的具体节点,明确和解聚散法的配伍、用量,明确TGF-β的平衡调节,有效延缓糖尿病肾病肾间质纤维化,进一步发展"肾络癥瘕聚散消长"病机理论。