面部瘢痕整形手术后胶布减张治疗疗效观察

目的:探讨使用免缝胶布对伤口减张制动的方法和治疗效果。方法:对62例面部瘢痕或痣切除手术患者,随机分组,45例为治疗组,术后5天拆线,拆线当天开始使用免缝胶布。胶布垂直跨越伤口线粘贴,使创缘处于无张力状态。17例为对照组,未使用免缝胶布。术后6个月对瘢痕进行临床评分,治疗组与对照组对比,统计分析。结果:62例患者伤口均一期愈合,治疗组37位(82%)、对照组4位(23%)患者瘢痕恢复至成熟期,没有痒或刺痛的主观症状,瘢痕颜色淡、平坦、质地软;治疗组5位(11.1%)、对照组13位(76.5%)患者瘢痕增宽,有痒或刺痛的症状,瘢痕颜色红,突出皮面。结论:免缝胶布可以有效地减轻伤口两侧张力,减少瘢痕增生,是一种瘢痕术后重要的辅助治疗。     

超声检查在男性创伤性尿道狭窄诊断中的价值

目的探讨超声检查对男性创伤性尿道狭窄的诊断价值。方法对21例男性创伤性尿道狭窄患者进行超声检查,14例同时联合尿道造影检查,结果与术中所见进行对比分析。结果21例超声结果与术中所见一致。14例联合检查中,造影检查有2例前尿道狭窄漏诊,2例狭窄近侧结石未发现,2例正常后尿道误诊为狭窄,2例后尿道狭窄长度不能估计;2例会阴部瘘管造影清晰显示而超声显示不佳。结论超声在狭窄定位、长度测量、瘢痕范围估测及显示继发结石等方面优于造影,造影显示瘘管清晰,必要时两者应联合应用。     

突触功能必需基因参与线虫衰老的调控(英文)

目的鉴定参与线虫衰老的神经内分泌调控的新基因。方法鉴于神经系统在衰老调控中的重要作用,通过寿命分析和脂褐质自发荧光的检测,从编码突触蛋白的遗传位点中筛选参与衰老调控的基因。我们还进一步检查了这些遗传位点相应的突变体的永久性幼虫形成情况,探讨它们是否可能受胰岛素样信号通路的调控。结果遗传位点 unc-10,syd-2,hlb-1,dlk-1,mkk-4,scd-2,snb-1,ric-4,nrx-1,unc-13,sbt-1,unc-64 可能参与线虫衰老的调控。而且在衰老的调控中,unc-10,syd-2,hlb-1,dlk-1,mkk-4,scd-2,snb-1,ric-4,nrx-1 的功能可能与unc-13,sbt-1,unc-64相反。肠道脂褐质自发荧光的检测进一步证明了筛选出的各基因对应突变体的长寿或短寿表型,是由减慢或缩短的组织衰老所致。在筛选出的基因中,syd-2,hlb-1,mkk-4,scd-2,snb-1,ric-4,unc-64 也参与了永久性幼虫形成的调控。另外,daf-2突变增强了syd-2和hlb-1的表达,降低了mkk-4,nrx-1,ric-4,sbt-1,rpm-1,unc-10,dlk-1,unc-13 的表达。daf-16突变提高了syd-2和 hlb-1 的表达,降低了mkk-4,nrx-1,sbt-1,rpm-1,unc-10,dlk-1,unc-13 的表达. 结论突触功能可能在个体寿命和永久性幼虫形成的调控机制中具有重要的作用。     

Abscess formation after transobturator sling placement: a case report

New techniques of sling placement may be associated with infectious complications. Slings through the obturator foramen and thigh can lead to a significant abscess formation within the thigh adductor muscles. A large thigh abscess associated with a transobturator sling was diagnosed and treated. The authors report the evaluation and treatment of a unique infectious complication of transobturator slings.     

葡萄球菌的原生质体融合及遗传转移

本文报道在金黄色葡萄球菌多剂抗生素耐药菌株(22)及白色葡萄球菌(Rif~r,Nal~r)间进行的原生质体融合试验。在高渗缓冲培养基中,将对数生长期中、后期的培养物,以溶菌酶及溶葡萄球菌素处理,DM_3培养基再生得原生质体。将二亲本葡萄球菌的原生质体混合,以CaCl_2及PEG(6000)处理使发生融合及种间遗传转移。根据重组子的色素、耐药性及在琼脂糖凝胶电泳中显示的质粒带进行遗传分析,证明在金黄色葡萄球菌及白色葡萄球菌中,质粒和染色体的遗传标记均可相互转移,再次证实在金黄色葡萄球菌(22)中存在分子量为2.0Mdal的pcr质粒。     

尿道损伤46例临床分析

本文报道尿道损伤46例,占同期泌尿系损伤的70%。分析尿道损伤手术术式和疗效,以尿道对端吻合最好.尿道会师牵引术亦有其优点。     

Elevated serum levels of the apoptosis related molecules TNF-α, Fas/Apo-1 and Bcl-2 in oral lichen planus

BACKGROUND: The serum circulatory levels of apoptosis related molecules measured in patients with oral lichen planus (OLP) and healthy individuals in order to investigate possible alterations associated with the clinical forms of OLP. METHODS: Serum levels of tumor necrosis factor (TNF)-alpha, soluble Fas (sFas) and Bcl-2 studied by enzyme-linked immunosorbent assay in whole blood samples in 13 OLP reticular, 13 OLP atrophic-erosive form patients and 26 healthy subjects. RESULTS: Significantly elevated levels of TNF-alpha and sFas detected in OLP patients as compared with controls. Serum concentrations of Bcl-2 although increased in 17/26 patients, they were not statistically significant. Reticular OLP exhibited slightly elevated TNF-alpha and significantly elevated Bcl-2 serum levels, compared with erosive OLP. CONCLUSIONS: These data suggest that a putative dysfunction in the Fas/FasL mediated apoptosis might be involved in the OLP pathogenesis. A downregulation of Bcl-2 serum levels in the atrophic-erosive OLP may be associated with promotion of the disease activity.     

The value of serial determination of MEGX and hyaluronic acid early after orthotopic liver transplantation

Abstract. Post-transplant assessment of early graft function has become an essential part of monitoring, especially when deciding on retransplantation. If primary non-function is indicated, retransplantation is inevitable; early graft dysfunction may be related to subsequent complications. In a prospective study in 84 patients after orthotopic liver transplantation (OLT) we measured aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamate dehydrogenase (GLDH), bilirubin (BIL), prothrombin time, MEGX formation, hyaluronic acid (HA) and soluble interleukin-2 receptor (sIL-2R) concentrations during the first 2 postoperative weeks; graft outcome was followed over 4 months. The aim of this study was to determine whether graft survival could be predicted by such variables early after OLT. Compared with patients with stable graft function (n= 25), patients with post-transplant icteric cholestasis (n= 30) exhibited no difference in graft survival, despite a decrease in MEGX formation to a nadir median of 12 μgL^-1 on day 10. Patients with rejection (n= 8) and septicaemia (n= 6) showed a marked decrease in MEGX values and an increase in HA and sIL-2R concentrations between postoperative days 3 and 7. Patients with primary non-function (PNF; n= 5) were characterized by strongly reduced MEGX formation (median 4 μgL) and increased HA values (median 2300 μgL^-1) on day 3 after OLT. A total of 24/84 grafts were lost within 120 days. In a survival analysis using the Cox proportional hazards regression, HA and MEGX values on day 1 were the only independent variables entering the model that showed an adequate prognostic sensitivity. At cut-off points of 22 μgL^-1 (MEGX) and 730 μgL^-1 (HA) the combined use of these parameters in a parallel approach yielded a sensitivity of 58% with a corresponding specificity of 95% for 120-day graft survival. These findings suggest that the inclusion of MEGX and HA in postoperative monitoring of OLT patients may be helpful in the early prediction of graft survival.     

rhBMP-2对不同培养时间骨髓基质干细胞成骨能力的影响

目的：探讨不同培养阶段骨髓基质干细胞成骨能力的变化及BMP-2对其成骨能力的影响。方法：培养兔骨髓基质干细胞，测定第3代和第18代细胞碱性磷酸酶及骨钙素活性；测定BMP-2对不同培养时间骨髓基质干细胞成骨能力的影响；测定rhBMP-2对细胞增殖的影响。结果：细胞传至第18代后，分泌的骨钙素（OC）水平及ALP活力明显降低，与第3代细胞相比，差异显著（P〈0．05）。第3代细胞在rhBMP-2的诱导下，分泌的OC水平及ALP活力在原基础上进一步升高，与对照组相比，差异显著（P〈0.05）。结第18代细胞在rhBMP-2的诱导下，分泌的OC水平及ALP活力在原基础上升高，与对照组相比，差异不显著（P〉0．05）；随培养时间的延长，各组细胞数量均有所增加，rhBMP-2诱导组与对照组细胞无显著性差异（P〉0．05）。细胞的传代次数对细胞增殖无明显影响（P〉0．05）。结论：rhBMP-2对细胞增殖无影响。随着传代次数的增加，骨髓基质干细胞的成骨能力下降，rhBMP-2促进其成骨的能力亦下降。