Direct infant UV light exposure is associated with eczema and immune development

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Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection

The application of reversed-phase gradient high-performance liquid chromatography with UV detection to the determination of cortisol and cortisone in 24-h urine samples is described. The method employs Sep-pak C18 cartridges for the part-purification and concentration of the corticosteroids, with sample enrichment at the head of an HPLC pre-column and separation using water/acetonitrile gradient. The internal standard is 6alpha-methylprednisolone. Measurement of both cortisone and cortisol provides further information on adrenocortical function. 24-hour excretion rate data from normal subjects are reported.     

The temperature dependence of derivative ultraviolet absorption spectra

The effects of variation of temperature in the range 0 degrees -40 degrees C on the zero and second derivative ultraviolet absorption spectra of ten compounds have been investigated. When the temperature of the solutions was increased, most of the substances showed a linear reduction of maximum absorbance (A(max)), with temperature coefficients of -0.07 +/- 0.03% per degree. The second derivative amplitudes of all the substances were reduced, with temperature coefficients (-0.1 to -0.5% per degree) that bore no significant relationship to those of the corresponding A(max) values. These effects on the extrema of the derivative spectra are explained by the small reduction in curvature at the corresponding wavelengths of the fundamental spectra, that occurs with increasing temperature. The precise and accurate assay of substances by derivative UV spectrophotometry requires that the temperatures of the standard and sample solutions are identical at the time of measurement.     

Primary intermediates of rhodopsin studied by low temperature spectrophotometry and laser photolysis. Bathorhodopsin, hypsorhodopsin and photorhodopsin

The primary photochemical processes of rhodopsin studied by low temperature spectrophotometry and picosecond laser spectroscopy in our group was summarized. Low temperature spectroscopic experiments demonstrated that the retinylidene chromophores of hypso- and bathorhodopsins are in a twisted all-trans forms. Excitation of rhodopsin with 532 nm laser pulse (width: 25 psec) yielded a new bathochromic photoproduct "photorhodopsin"; its spectrum was located at longer wavelengths than that of bathorhodopsin. Photorhodopsin decays to bathorhodopsin with time constants of about 200 psec in squid and 40 psec in cattle. Squid and octopus hypsorhodopsins were produced within 25 psec by high energy pulse, but not by low energy pulse. Thus hypsorhodopsin is produced by two photon reactions (sequential two photochemical reactions) and decayed to bathorhodopsin with time constant of 125 psec.     

四苯基卟啉锰配合物与牛血清白蛋白相互作用的研究

用分光光度法研究了四苯基卟啉锰配合物（Mn^3 TPPCl)与牛血清白蛋白（BSA）之间的相互作用,测定了二者相互作用的平衡常数和热力学参数,发现二者之间可形成1：1的配合物,BSA存在一个强结合位。在它们的相互作用过程中发生氧化还原反应,一个电子上BSA转移至MnTPPCl。     

复方补血胶囊中葡萄糖酸亚铁的含量测定

目的：复方补血胶囊中葡萄糖酸亚铁的含量测定。方法：根据Fe^2 与邻二氮菲显色,在510nm处有最大吸收,利用分光光度法测定。结果：葡萄糖亚铁在7－34μg/ml的浓度范围内线性关系良好（r=0.9999),回收率、日内变异均符合要求,结论：建立了复方补血胶囊中葡萄糖酸亚铁的含量测定方法。     

肝硬化患者血清一氧化氮水平测定及其临床意义

目的：了解肝硬化患者血清一氧化氮（NO）水平的变化,并探讨其临床意义。方法：采用分光光度法检测64例肝硬化患者及30例正常人血清NO水平。结果：肝硬化患者血清NO水平显著升高（P＜0．01）,而且其升高水平在失代偿、有腹水、肝功能分级差,伴有并发症的患者较代偿、无腹水、肝功能分级好,不伴有并发症的患者明显（0．01＜P＜0．05）。结论：肝硬化患者血清NO水平升高,可能与疾病活动情况、腹水形成、肝功损害,并发症发生及预后等有关。     

紫外光谱法测定利巴韦利葡萄糖注射液中利巴韦林的含量

目的建立利巴韦林葡萄糖注射液中利巴韦林的快速测定方法.方法用紫外分光光度法直接测定利巴韦林葡萄糖注射液中利巴韦林的含量.结果在207 nm处,以水为溶媒,利巴韦林葡萄糖注射液中利巴韦林浓度在3～15μg/ml,与其相应的吸收度呈良好的线性关系(n=5;r=0.999 9),样品平均回收率为101.4%±0.53%.结论该法简便快速、准确可靠,适合药房快速分析.     

正交试验法筛选口服阿苯达唑毫微球制备工艺

目的为提高阿苯达唑生物利用度,采用α-氰基丙烯酸正丁酯为聚合材料,乳化聚合法制备口服阿苯达唑毫微球,并优化处方工艺.方法紫外分光光度法测定阿苯达唑含量,以包封率为主要指标,乳化聚合二步法制备阿苯达唑毫微球,L9(34)正交试验设计处方工艺.结果首先制备pH为5.0的聚氰基丙烯酸正丁酯空白毫微球,再以0.5 ml/min速率缓慢将空白毫微球注入同体积的阿苯达唑醋酸溶液中,充分搅拌16 h.结论经过优化筛选的组方工艺制备的阿苯达唑毫微球包封率为(61.01士4.06)%,载药量为(48.00士5.20)%.