管状胃经胸骨后与食管床不同上提路径对食管癌患者手术并发症的影响

目的：分析胸腹腔镜食管胃颈部吻合术中管状胃经胸骨后与食管床不同上提路径对食管癌患者手术并发症的影响。方法按照胸腹腔镜食管癌切除、胃颈部吻合术中不同上提路径将85例确诊食管癌患者分为食管床组42例和胸骨后组43例,术后观察两组患者的手术指标和并发症发生情况。结果两组患者手术时间、术中出血量、住院时间、吻合口瘘、吻合口狭窄、呼吸衰竭、心律失常和其他并发症发生率比较,P〉0.05;胸骨后组胃管留置时间、胃液引流总量、肺部感染和胸腔感染发生率明显低于食管床组,P〈0.01或P〈0.05。结论胸腹腔镜食管胃颈部吻合术中管状胃经胸骨后上提路径治疗食管癌效果确切,能够减少胃管留置时间和胃液引流总量,降低术后肺部感染和胸腔感染的发生率。     

The syndrome of hypertension and hyperkalaemia with normal glomerular function (Gordon's syndrome)

A 14-year-old boy with the syndrome of hypertension and hyperkalaemia with normal glomerular filtration rate (Gordon's syndrome) is described. The patient's clinical symptoms consisted of periodic paralysis, slight metabolic acidosis of the proximal type and hypercalciuria. Prostaglandin excretion was normal. Infusion of atrial natriuretic peptide had no effect on electrolyte excretion or glomerular function although a normal increase in cyclic guanosine monophosphate was demonstrated in plasma and urine. This lack of sensitivity to atrial natriuretic peptide offers a new pathophysiological concept in this syndrome. Treatment with hydrochlorothiazide was successful in this case.     

Clinical Applications of the Tubular Retractor on Spinal Disorders

Tubular retractor system as a minimally invasive surgery (MIS) technique has many advantages over other conventional MIS techniques. It offers direct visualization of the operative field, anatomical familiarity to spine surgeons, and minimizing tissue trauma. With technical advancement, many spinal pathologies are being treated using this system. Namely, herniated discs, lumbar and cervical stenosis, synovial cysts, lumbar instability, trauma, and even some intraspinal tumors have all been treated through tubular retractor system. Flexible arm and easy change of the tube direction are particularly useful in contralateral spinal decompression from an ipsilateral approach. Careful attention to surgical technique through narrow space will ensure that complications are minimized and will provide improved outcomes. However, understanding detailed anatomies and keeping precise surgical orientation are essential for this technique. Authors present the technical feasibility and initial results of use a tubular retractor system as a minimally invasive technique for variaties of spinal disorders with a review of literature.     

A micropuncture study of the effect of isoprenaline on renal tubular fluid and electrolyte transport in the rat

Summary To explain the mechanism of the isoprenaline induced antidiuresis the effect of this substance on fluid and electrolyte reabsorption in various nephron segments of the rat kidney has been studied using micropuncture techniques. Isoprenaline (1.5×10^–9 mol/kg·min i.v.) decreased GFR of superficial nephrons and increased fractional proximal fluid, sodium and potassium reabsorption. However, the fractions of filtered fluid and electrolytes, which had been reabsorbed up to the early distal tubule were unchanged after isoprenaline. This indicates that the increased fractional proximal reabsorption has been completely compensated by decreased reabsorption from Henle's loops. In the distal convoluted tubules an increase in the fractional fluid and sodium reabsorption could be established using a recollection technique. Given directly into the distal tubular lumen isoprenaline stimulated the isotonic fluid reabsorption, which was measured by the Gertz split oil droplet method.From these results it is concluded that the decrease in superficial GFR and the increased distal tubular fluid and sodium reabsorption lead to the decrease in urine volume and in urinary electrolyte excretion after isoprenaline.with the technical assistance of U. Greven and S. HildebrandSupported by Deutsche ForschungsgemeinschaftPortions of this work were presented at the Meeting of the Deutsche Pharmakologische Gesellschaft in Graz, 1974 (Greven, 1974b)     

血管组织工程基质材料及管形支架的制备

目的：几种血管基质材料的制作及比较。方法：采用EDC交联胶原和粘多糖材料；采用戊二醛和热交联得到明胶加壳聚糖材料；应用去垢剂，渗透压改变和核酸酶消化的方法，对猪主动脉进行去细胞处理，得到完整的去细胞纤维支架。采用直接模具成型，形成多种管形支架。结果：得到明胶加壳聚糖、完整的猪主动脉去细胞纤维支架等几种基质材料；管形支架外径1cm，内径0．6～0．8cm，放置培养液中10d，可保持管腔形状，不塌陷，不变形。结论：明胶加壳聚糖三维结构好，主动脉脱细胞基质仍显致密，采用模具直接成型、冷冻干燥技术相结合，可以得到多种管状的可降解基质支架。     

Honeycomb-like tubular structure in axoplasm

Summary A honeycomb-like tubular array was observed in heavily myelinated axons. It was observed in the cerebellum of an apparently normal rat and a chimpanzee with Kurulike symptoms. It was also found in the cerebral cortex of a mouse that had had a intracerebral tumor implant. A common underlying etiology could not be found. It is suggested, however, that this structure represents a specific axonal response to perhaps a variety of as yet unknown conditions.This work was done during the tenur of United States Public Health Service, National Cancer Institute, Special Fellowship Award No. IF3-CA-31, 147-Ol.     

Coiled nucleocapsid configuration in subacute sclerosing panencephalitis (SSPE)

Summary In this case of documented subacute sclerosing panencephalitis (SSPE), electron microscopy of a brain biopsy revealed a previously unreported fingerprint configuration of tubular inclusions or nucleocapsids. The pattern resembled that of the whorled filamentous nuclear bodies which are so frequently encountered in ultrastructural studies of this disease. Furthermore, an apparent tubule was seen running parallel to filaments within the nucleus of still another affected cell. These findings lend support to the concept that nuclear bodies are converted into and/or synthesize nucleocapsids.     

Honeycomb-like intra-axonal tubular structures in irradiated rat cerebella

Summary In the cerebella of all (5) 30-days old rats, whose cerebellar region was irradiated with 1000 r at the 2nd day of life, honeycomblike tubular arrays were found in myelinated axons, identical with those described by others in a variety of conditions.

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Zusammenfassung Im Kleinhirn aller (5) 30 Tage alter Ratten, deren Kleinhirnregion mit 1000r am Ende des 2. Lebenstages bestrahlt worden war, fanden sich honigwabenartige tubuläre Formationen in den bemarkten Axonen, die den von anderen Autoren bei verschiedenen Bedingungen beschriebenen Veränderungen entsprechen.

     

分离纯化肾小管基底膜的改进方法

本实验采用分样筛、磁性分离和密度梯度离心综合法分离猪肾皮质的肾小管基底膜。相差显微镜观察肾小管的纯度可达97％以上。磁性分离后用超声震碎细胞核,再经密度梯度离心,即可获得高纯度的肾小管基底膜。