学习障碍患儿血清BDNF水平的研究

目的探讨学习障碍血清脑源性神经营养因子(BDNF)水平的变化,及其与学习障碍病理基础的关系。方法患者组为22例未治疗过且不共患其他疾病的学习障碍患儿,对照组为16例年龄性别匹配正常儿童,以瑞文标准推理测验(SPM)测定智商,BDNF采用酶联夹心免疫吸附法检测。结果LD患者血清BDNF浓度为:平均(4.603±3.620)ng/ml高于对照组(1.843±0.728)ng/ml(t=3.326,P=0.003<0.01)。边缘智商组(4.523±4.618)ng/ml,与正常对照组比较差异有非常显著性(P=0.008<0.01)。结论学习障碍患儿血清BDNF浓度高于正常儿童,边缘智商者升高更明显。     

老年人血清胆红素水平与冠状动脉病变程度的关系

目的观察老年人血清胆红素与冠状动脉病变的关系。方法经冠状动脉造影确诊的冠心病患者80例,其中冠状动脉轻度狭窄组15例,中度狭窄组32例,重度狭窄组33例;另外选取冠状动脉造影正常者40例做对照。采用重氮苯磺酸法测定血清胆红素水平,并对胆红素与各因素进行直线相关分析。结果冠心病组血清胆红素水平明显低于正常对照组,血清胆红素水平与冠状动脉病变程度成显著负相关关系(r=-0.26,P<0.05)。结论冠心病患者血清胆红素水平较正常人明显降低。低血清胆红素可能是冠心病的一种危险因素。血清胆红素水平与冠状动脉狭窄程度有明显相关性。     

原发性高血压病患者颈动脉粥样硬化程度与血清胆红素水平的相关性研究

目的 探讨高血压病患者颈动脉粥样硬化程度与血清胆红素水平的关系.方法 158 例高血压病患者行双侧颈动脉超声多普勒检查,根据检测结果分为颈动脉粥样硬化组和正常颈动脉组.记录各组年龄、性别、体重指数、吸烟史、糖尿病史和血压,测定血清总胆红素及其他生物化学指标.结果 颈动脉粥样硬化组的血清总胆红素水平(8.37±3.53)μmol/L显著低于正常颈动脉组(14.53±4.61)μmol/L(P＜0.05).多因素回归分析显示低胆红素血症与颈动脉粥样硬化呈显著正相关(χ2wald=4.41,P=0.029,OR=3.72).结论 低胆红素血症是高血压病患者发生颈动脉粥样硬化的独立危险因素.     

大鼠气管上皮细胞体内—体外转化模型的研究

本研究建立了大鼠气管上皮细胞体内－体外转化模型，大鼠气管内滴注苯并芘，三天后处死大鼠，消化气管上皮细胞，接种于无血清完全培养基。细胞形成集落后，换为选择培养基继续培养五周，统计转化率。结果显示，２５ｍｇ／ｋｇ和５０ｍｇ／ｋｇ的苯并芘可诱导大鼠气管上皮细胞转化及微核增加，用同样方法研究了煤焦沥青提取物，结果表明，剂量为８ｍｇ／ｋｇ和２５ｍｇ／ｋｇ的煤焦沥青提取物能明显诱导大鼠气管上皮细胞转化。     

庆大霉素经乳汁的排泄

采用荧光偏振免疫法测定１４例剖宫产术后应用肌注或静点庆大霉素的产妇乳汁及血清中庆大霉素的浓度，结果表明肌注或静点庆大霉素后３￣５小时，乳汁中浓度达峰值，血清中庆大霉素的浓度于给药后０．５小时达峰值。提示：无论肌注或静脉给庆大霉素均可经乳汁排泄，乳母不宜应用庆大霉素，即使产后已应用庆大霉素，应停药１３小时后方可哺乳。     

血清磷、肌酸激酶及其同工酶的测定在肠梗阻诊断中的应用

肠梗阻是临床常见的急腹症，绞窄性肠梗阻的死亡率可高达４５％～３０％，我们自１９９５年８月至１９９７年４月共收治肠梗阻５３例，其中单纯性肠梗阻２８例，经手术证实为绞窄性肠梗阻２５例（其中肠坏死８例），在治疗前均测定血清磷、肌酸激酶及其同工酶，其结果：本组病例中血清磷升高者１４例，其中以８例肠坏死为显著，肌酸激酶及其同功酶的升高也以绞窄性肠梗阻为明显，其中又以发病时间在４８小时以内者和８例肠坏死者为著，因此，我们认为：血清磷、肌酸激酶及其同工酶的测定可以作为判断肠管是否发生绞窄及坏死的辅助诊断指标     

银屑病患者血清促进皮肤成纤维细胞增生的实验研究

进行期寻常型银屑病患者混合血清以5%的比例加入培养基中,作用于体外培养的正常人皮肤成纤维细胞.结果发现,对该细胞的增生具有明显的促进作用.提示银屑病不仅是表皮细胞的异常增生,真皮成纤维细胞的异常和系统性因素在其病理生理方面也占有重要地位.     

Sialic acid concentrations in the urine of men with and without renal stones

Summary It has been suggested that urinary sialidase may play a role in the formation of renal stones. The present study was therefore undertaken to compare spectrophotometrically the different types of sialic acid concentrations and sialidase activities in fresh first morning urine specimens of men (21–65 years) with (13) and without (9) calcium oxalate renal stones. Although the free urinary sialic acid concentrations of the two groups of men were statistically about the same (P=0.0614), the total (P=0.003) and bound (P=0.0012) urinary sialic acid concentrations differed significantly. Both the total and bound sialic acid concentrations were lower in the urine specimens of the stone patients than in their healthy counterparts. This decrease in urinary sialic acid concentrations was firstly thought to be the result of elevated breakdown enzymes of sialic acid, which would favour the production of pyruvate. However, spectrophotometric determinations of the endogenous pyruvate concentrations of the two types of urine specimens did not differ significantly (P=0.0708). Secondly, the decrease in total urinary total sialic acid concentration of stone patients, could be attributed to less sialic acid synthesis or less renal excretion. Therefore, the same experiments were repeated using serum of 13 patients and 9 healthy men. Conversely, the total (P=0.4425) and bound (P=0.2850) serum sialic acid concentrations were found to be similar in the two types of subjects. However, the free serum sialic acid concentration of stone patients was significantly lower than in the healthy subjects (P=0.0062). This phenomenon is also reflected in the average ratio for serum free: bound sialic acid in healthy and stone patients, 1:7.9 and 1:18.7 respectively (P=0.0009). The lower free serum sialic acid concentration may lead to lower renal excretions of sialic acid. This may explain the decrease in total urinary sialic acid concentration in stone patients. The lower bound urinary sialic acid concentrations in patients was also reflected in the urinary free: bound sialic acid ratio for healthy (1:2.3) and stone patients (1:1.3). The difference between these two groups of men was highly significant (P=0.0001). This phenomenon might be explained by the urinary sialidase activities, which was spectrophotometrically determined at 334 nm at 37°C of 11 patients with stones and 17 healthy men. The ages of both groups of men were the same (P=0.326). An increase in urinary sialidase activity was observed with the stone patients (P=0.00001) when compared to specimens of healthy men. This might explain the decrease in urinary bound sialic acid concentration of the stone group. It seems from these results that the urinary concentration of sialic acid and the activity of urinary sialidase, may play a role in the pathogenesis of the multifactorial disease, urolithiasis.     

乌司他汀对重症急性胰腺炎血浆内毒素水平的影响及其疗效观察

目的　观察乌司他汀治疗重症急性胰腺炎对血清内毒素水平的影响。方法　 4 0例重症急性胰腺炎患者随机分为乌司他汀治疗组和一般常规治疗组两组 ,两组患者住院后分别在第 1、4、7、10天抽血测定血清淀粉酶、血浆内毒素水平 ,结合APACHEⅡ评分系统分析观察乌司他汀的临床疗效。结果　重症急性胰腺炎入院后第 1天血浆内毒素水平明显高于正常健康对照组 ,分别为 (1.10± 0 .2 3)IU/L和 (0 .2 0± 0 .5 0 )IU/L(P <0 0 0 1) ,治疗组血清淀粉酶、血浆内毒素水平下降均较对照组明显 (P <0 0 1) ,住院第 7天APACHEⅡ评分值低于对照组 (P <0 0 5 )。结论　重症急性胰腺炎患者早期存在不同程度的内毒素血症 ,乌司他汀治疗重症急性胰腺炎疗效肯定 ,并能降低患者血浆内毒素水平和改善预后     

子宫肌瘤患者外周静脉与子宫静脉血管内皮细胞生长因子水平测定及临床意义

目的:探讨血管内皮细胞生长因子(VEGF)在子宫肌瘤发生和肌瘤生长中的作用。方法:手术中暴露子宫静脉后,抽取子宫静脉血3ml,同时抽取非输液肢体外周静脉血3ml。抽取配对正常妇女外周静脉血3ml。ELISA法测定VEGF水平。测量子宫重量、肌瘤总重量、肌瘤数目和最大肌瘤最大径线值。结果:子宫肌瘤患者子宫静脉血和外周静脉血VEGF水平分别为159.38±25.63pg/ml和76.35±26.61pg/ml,对照人群外周静脉血VEGF水平为42.53±29.47pg/ml。子宫静脉血中的VEGF水平明显高于外周静脉血(P=0.019),子宫肌瘤患者外周静脉血中的VEGF水平明显高于对照人群外周静脉血的VEGF水平(P=0.013)。子宫重量、肌瘤总重量、肌瘤数目、最大肌瘤最大径线都与子宫肌瘤患者子宫静脉和外周静脉VEGF呈显著正相关。结论:VEGF可能参与了子宫肌瘤的生长和病情发展。