新城疫病毒合用中药黄芪诱生内源性干扰素的研究

目的研究新城疫病毒（NDV）合用中药黄芪在小鼠体内诱生内源性干扰素-α及对小鼠的免疫调节作用。方法实验分3部分,实验1：分别以NDV、黄芪及二者合用予小鼠口腔喷洒给药,连续10日后以ELISA法测定小鼠唾液中干扰素-α和SIgA的含量;实验2：分别以NDV、黄芪及二者合用予小鼠腹腔注射给药（黄芪灌胃方式给药）,ELISA法测定给药10日后血清干扰素-α的含量;实验3：分为NDV、黄芪及二者合用的高、中、低三种剂量,给药方法同第二组,6天后MTT法测定脾脏NK细胞的活性。每组分别设置阴性对照。结果NDV和黄芪口腔给药能升高小鼠唾液干扰素-α（35.54±10.20IU/ml,5.69±1.35IU/ml）和SIgA含量（68.88±15.47ng/ml,44.32±9.87ng/ml）,腹腔给药能诱生血清干扰素-α（120.54±29.21IU/ml,10.69±6.35IU/ml）,并能激活NK细胞活性（50.4%,32.1%）。NDV合用黄芪组的诱生效果远高于单独使用组（98.55±16.95IU/ml,101.31±27.59ng/ml,262.55±26.91IU/ml,75.6%）,显示出较强的协同作用。结论NDV合用黄芪能诱生小鼠内源性干扰素,并能发挥对小鼠SIgA和NK细胞的免疫调节作用。     

无龋儿童和患龋儿童唾液中免疫成分的比较

目的 :对无龋儿童和患龋儿童唾液中免疫成分进行检测 ,研究龋病与唾液中免疫成分的关系。方法 :对 2 0 4名 4～ 6岁幼儿园儿童进行口腔检查 ,收集混合唾液测定 pH值、SIgA和溶菌酶的含量 ,并对无龋儿童和患龋儿童唾液免疫成分进行比较。结果 :2 0 4名儿童中 ,无龋 5 9名。患龋 14 5名 ,患龋率 71.0 8%。患龋儿童龋均5 .2 6,受检儿童龋均 3 .74。患龋者唾液 pH 7.0 4± 0 .3 7,无龋者唾液 pH 7.14± 0 .3 9,两组比较无显著性差异 (P >0 .0 5 )。患龋者唾液SIgA为 5 8.81± 40 .76μg/ml ,无龋者SIgA为 48.61± 2 5 .83 μg/ml,两者比较具有显著性差异(P <0 .0 5 )。患龋者唾液溶菌酶含量 18.63± 2 0 .64 μg/ml ,无龋者溶菌酶含量 13 .77± 12 .2 5 μg/ml ,两者比较具有显著性差异 (P <0 .0 5 )。结论 :无龋儿童与患龋儿童唾液 pH值无明显差异 ,患龋儿童SIgA及溶菌酶含量较无龋儿童低 ,SIgA及溶菌酶在龋病发病中起重要作用。     

血清和骨髓IgG,SIgA,铁蛋白和胃动素在缺铁性贫血患者中的变化

目的：为探讨缺铁性贫血（ＩＤＡ）时机体免疫及铁蛋白改变,测定了ＩＤＡ患血清和骨髓ＩｇＧ,ＳＩｇＡ,铁蛋白（ＦＲＴ）和胃动素（ＭＴＬ０含量并讨论其临床意义。方法：ＩＤＡ患２２例,男９例,女１３例,平均年龄３５．８岁,均经临床,周围血象和骨髓象检查确诊,并排除其它原因引起的缺铁性贫血,血清和骨髓ＩｇＧ,ＳＩｇＡ,ＦＲＴ和ＭＴＬ含量用放射免疫法测定,结果经统计学处理。     

Interest of immunomodulation as a mean to improve the preparation of polyclonal and monoclonal antibody reagents

Immunomodulation by monoclonal antibodies (mAbs) was investigated in mice in order to improve the preparation of antibody reagents. Three different types of representative immunogens were chosen: a human soluble protein (secretory immunoglobulin A, SIgA), a bacterial polysaccharide from E. coli K1 and an envelope protein from the hepatitis B virus. These Ag are all of importance for diagnosis and exhibit different levels of immunogenicity. Antibody-mediated enhancement was observed against restricted and defined regions of each immunogen i.e.: the Fab epitopes of SIgA, the preS1 domain of the HBV envelope and associated cell wall components of the capsular PS. The epitopes which were enhanced appeared to be different from those recognized by the modulating mAb. Negative modulations were also observed. Moreover, new epitopes seemed to be generated. In both cases the level and direction of the modulation were irrespective of isotypy and affinity of the mAbs. Interestingly the positive modulatory effect was found to be correlated with an in vitro assay based on the binding of immune complex to antigen-presenting cells.     

化痰止喘汤对慢性阻塞性肺病患者血清SIgA的影响

目的 :观察化痰止喘汤对慢性阻塞性肺病患者血清SIgA的影响。方法 :在常规治疗的基础上 ,治疗组服用化痰止喘汤 ,4周为 1疗程 ,分别于治疗前及疗程结束第 2天 ,两组同时抽取静脉血 2ml,测定血清中SIgA含量变化。结果 :治疗组治疗后血清SIgA值变化较治疗前有显著差异 (P <0 .0 1 ) ,且明显优于对照组 (P <0 .0 1 )。结论 :化痰止喘汤能提高慢性阻塞性肺病患者血清SIgA水平     

健脾止泻颗粒对脾虚泄泻患儿消化吸收与肠道局部免疫功能的影响

观察脾虚泄泻患儿消化吸收与肠道局部免疫功能以及健脾止泻颗粒的作用机制。方法对64例脾虚泄泻患儿用健脾止泻颗粒治疗,检测其治疗前后的唾液淀粉酶活性、尿D-木糖排泄率及大便SIgA,并与30例正常儿进行对照观察。结果脾虚泄泻患儿消化吸收与肠道局部免疫功能低下,经治疗后,上述指标明显好转。结论健脾止泻颗粒具有促进脾虚泄泻患儿消化吸收功能和提高肠道局部免疫功能的作用。     

SIgA在肠道免疫中的作用

分泌性免疫球蛋白A(SIgA)是由J链连接成的双聚IgA与SC(分泌片段)结合后形成的复合物,其分子量约为400kD。组成SIgA的三者之间互有作用,共同确保作为整体的SIgA能有效发挥免疫作用。此文就SIgA各组成部分的生成、调节及其在肠道发挥免疫保护作用的方式作一综述。     

A comprehensive understanding of the gut mucosal immune system in allergic inflammation

Despite its direct exposure to huge amounts of microorganisms and foreign and dietary antigens, the gut mucosa maintains intestinal homeostasis by utilizing the mucosal immune system. The gut mucosal immune system protects the host from the invasion of infectious pathogens and eliminates harmful non-self antigens, but it allows the cohabitation of commensal bacteria in the gut and the entry of dietary non-self antigens into the body via the mucosal surface. These physiological and immunological activities are regulated by the ingenious gut mucosal immune network, comprising such features as gut-associated lymphoid tissue, mucosal immune cells, cytokines, chemokines, antimicrobial peptides, secretory IgA, and commensal bacteria. The gut mucosal immune network keeps a fine tuned balance between active immunity (against pathogens and harmful non-self antigens) and immune tolerance (to commensal microbiota and dietary antigens), thus maintaining intestinal healthy homeostasis. Disruption of gut homeostasis results in persistent or severe gastrointestinal infection, inflammatory bowel disease, or allergic inflammation. In this review, we comprehensively introduce current knowledge of the gut mucosal immune system, focusing on its interaction with allergic inflammation.     

儿童反复呼吸道感染外周血Th17细胞、唾液中分泌型免疫球蛋白A/白蛋白比值变化及其意义

目的探讨儿童反复呼吸道感染(RRTIs)外周血Th17细胞、唾液中分泌型免疫球蛋白A(sIgA)与血清白蛋白(ALB)比值变化及其意义。方法 2014年10月至2015年7月广州中医药大学附属中山市中医院儿科收治RTTIs患儿100例,其中急性期和缓解期各50例。同期选取门诊体检的健康儿童50例为正常对照组。流式细胞仪检测Th17细胞表达率,酶标仪检测唾液中sIgA,免疫比浊法检测ALB。结果 RRTIs患儿急性期组、缓解期组外周血Th17细胞数较正常对照组患儿增高,差异有统计学意义(P0.05);RRTIs患儿急性期组外周血Th17细胞数高于缓解期组,差异有统计学意义(P0.05)。外周血中Th17阳性细胞数与呼吸道感染严重程度呈正相关(P0.05)。正常对照组唾液中sIgA与血清白蛋白比值显著高于RRTIs患儿急性期组、缓解期组,差异有统计学意义(P0.05);RRTIs患儿急性期组与缓解期组唾液中sIgA与血清白蛋白比值比较差异无统计学意义(P0.05)。结论 RRTIs患儿外周血Th17细胞表达增加,且与其病情的严重程度呈正相关。RRTIs患儿存在黏膜免疫功能低下,增强呼吸道免疫功能能从根本上减少RRTIs的发作。     

口服微生态制剂对直肠癌患者术后肠道菌群分布及SIgA的影响

目的探讨低位直肠癌患者行腹、会阴联合直肠癌根治术(Miles术)术后口服微生态制剂对肠道菌群及粪便分泌型免疫球蛋A(SigA)含量的影响。方法将126例Miles术后患者采用随机化原则分成观察组和对照组,每组各63例。观察组患者术后给予口服双歧三联活菌胶囊(培菲康胶囊),对照组常规治疗。分别于入院时、术后第一次排便、术后1个月收集两组患者的新鲜大便,比较两组患者的肠道菌群的改变及粪便SIgA含量。结果术后第一次排便及术后1月观察组双歧杆菌、乳酸杆菌、拟杆菌数量显著高于对照组(P0.05),而肠球菌、肠杆菌数量均显著低于对照组(P0.05)。术后1个月,观察组患者的肠道菌群与手术前相比,基本恢复(P0.05)。术前及术后第一次排便,两组患者粪便中的SIgA水平无显著差异(P0.05);而术后1个月,观察组粪便SIgA含量均显著高于对照组(P0.05),且恢复至术前水平,而对照组仍低于术前水平。结论直肠癌Miles手术后口服微生态制剂可有效纠正患者肠道菌群失调,并通过升高粪便SIgA含量恢复肠道屏障功能和局部免疫力。