牛磺酸对荷S_（180）小鼠的抑瘤作用

①目的研究牛磺酸对Ｓ１８０肉瘤小鼠的抑瘤作用及对肿瘤化疗的协同效应。②方法将小鼠分为饮水（对照组）和牛磺酸（实验组）两组，各组又分为不予治疗和进行化疗两组。接种Ｓ１８０肉瘤后，观察小鼠的存活时间、肉瘤大小及抑瘤率，并观察牛磺酸的抑瘤作用及与化疗是否具有协同效应。③结果在接种肿瘤后，对照组的存活时间与瘤重分别为１８．７±５．６ｄ和４．５４±２．４０ｇ，实验组则分别为３５．７±２３．７ｄ及２．４４±３．３３ｇ．在接种瘤细胞的第２天，腹腔一次性注射环磷酰胺后，对照组生存天数和瘤重分别为２９．８±１２．３ｄ和４．１７±３．２ｇ，而实验组生长到６１ｄ时仍无死亡者，也未见到肉眼肿块，两组间差异有极显著性（ｔ＝７．６２，Ｐ＜０．０１）。④结论牛磺酸能抑制肿瘤的生长，对化疗效果有协同效应。     

Comparison of lectin fromPseudixus japonicus and concanavalin a on lymphocytes proliferation and cytotoxicity

Pseudixus japonicus agglutinin (PJA) was isolated. And its characteristics were compared with those of concanavalin A (Con A). PJA is a glycoprotein composed of 49.3% carbohydrate and 50.7% protein which had relatively high percentages of glutamic acid, aspartic acid and phenylalanine residues. The hemagglutinating activity of PJA was approximately one-eighth of that of Con A when tested with mouse erythrocytes. PJA failed to stimulate the proliferation or transformation of human and mouse lymphocytes in contrast to Con A. PJA and Con A showed cytotoxicities against SNU-1 (human stomach cancer cells), SNU-C1 (human colon cancer cells) and mouse Sarcoma 180 cells when tested by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay. The antitumor activity of the lectinin vivo was also tested in Sarcoma 180 bearing mice. There was no significant difference in prolongation of life span of the mice after the treatment with PJA and Con A for 10 consecutive days.     

胡桃醌对肿瘤细胞的增殖抑制作用和抗菌作用

本文主要探讨胡桃醌对部分移植性肿瘤细胞增殖作用的影响。经72h的作用结果表明,胡桃醌对不同肿瘤细胞的增殖抑制浓度分别为:HeLa Cell IC_(50) 13.8μg/ml,P388 CellIC_(50) 9.8μg/ml,P388/ADR Cell IC_(50) 7.1μg/ml,S180 Cell IC_(50) 11.6μg/ml. 从胡桃醌对S_(180)细胞的增殖抑制作用,可以得出该化合物的作用方式有,杀伤细胞与浓度的依赖关系。     

Serotonin-lesion Myoclonic syndromes. I. Neurochemical profile and S-1 receptor binding

This paper and the following one describe the effects of L-5-hydroxytryptophan (5-HTP) (after 3 intracisternal injections of 5,7-dihydroxytryptamine (DHT], fenfluramine (FF), p-chloroamphetamine (PCA) and drug combinations on (i) brain regional amine concentration (HPLC with LEC) and serotonin S-1 receptor binding; and (ii) 'serotonergic' behaviors in the same adult rats. Serotonin (5-HT) neurotoxins produced significantly different regional profiles of 5-HT depletion. Multiple DHT injections caused a 90-100% depletion of 5-HT concurrently in neocortex, hippocampus, striatum, septum/accumbens, pons, cerebellum, and cervical cord. Only PCA significantly depleted midbrain. Drug combinations with DHT resembled DHT alone rather than additive depletions, except for PCA + DHT, which produced a hybrid pattern of depletion. The S-1 binding assay, using cold 5-HT to displace [3H]5-HT, was performed with and without ascorbate, EDTA, CaCl2, and pargyline. Without ascorbate, binding was specific, saturable, region-dependent, and non-linear with high (Kd 1-3 nM) and low affinity (10-20 nM) components but no cooperativity (0.8 less than nH less than 1.0). Bmax and Kd did not differ significantly between vehicle- and drug-treated animals in neocortex, hippocampus, striatum, thalamus, hypothalamus, midbrain, pons, medulla, cervical cord, cerebellum, or septum/accumbens two weeks after lesioning, while the assay did detect a 60% reduction in Bmax induced by ascorbic acid (1 mM). The effects of assay conditions exceeded the changes sometimes reported in S-1 receptor Bmax after 5-HT lesions.     

阿司匹林对S180肿瘤生长及血管生长相关因子表达的抑制作用

目的　从抗血管生成的角度研究阿司匹林(Asp)预防和治疗肿瘤的机理。方法　昆明种小鼠随机分为对照组 ,替加氟组 ,Asp 5 0 ,2 5 ,10mg·kg- 1组 ,于接种S180肿瘤细胞后d 2开始ig给药 ,连续9d ,观察抑瘤率。采用免疫组化的方法研究Asp对肿瘤组织环氧合酶 2 (COX 2 )及血管相关生长因子的作用。结果　Asp 3个剂量组均有一定的抑瘤作用 ,大剂量抑瘤率为 2 1.1%。免疫组化染色显示Asp对肿瘤组织的COX 2表达有明显的抑制作用 ,同时血管生长相关因子血管内皮生长因子、纤维细胞生长因子 2表达也明显下调 ,肿瘤组织微血管密度明显降低。结论　Asp对S180肿瘤有抑制作用 ,它可以抑制与血管生长相关的COX 2的表达而抑制肿瘤血管的生长 ,进而抑制肿瘤的生长。抑制肿瘤血管的生长可能是Asp预防和治疗肿瘤的机理之一。     

地黄多糖b对正常及S180荷瘤小鼠T淋巴细胞功能的影响

地黄多糖ｂ，在体内与体外实验中能明显提高正常小鼠Ｔ淋巴细胞的增殖反应能力，促进白介素２的分泌，显示了明显的元疫调节活性．在对应于其产生明显抑瘤作用的时相里，能相对改善荷瘤小鼠由于肿瘤生长引起的白介素２分泌功能的下降，以及显著的提高此时细胞毒性Ｔ淋巴细胞（ＣＴＬ）的活力。因此认识到地黄多糖ｂ增强ＣＴＬ对肿瘤细胞的杀伤效应功能是其产生抑瘤作用的一个重要途径。     

S180荷瘤小鼠血液中指标的变化

目的:通过对S180荷瘤小鼠血液中白细胞分类、血生化、T细胞亚群和各种酶类等指标的测定,确定S180荷瘤小鼠血液中各项指标的改变情况,为进一步的抗肿瘤试验提供指标选择的依据。方法:建立S180小鼠实体瘤模型,采用全自动生化分析仪、血球计数仪和流式细胞仪等测定荷瘤小鼠血中的总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白/球(A/G)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、葡萄糖(GLU)、尿素氮(BUN)、肌酐(CR)、甘油三酯(TG)、总胆固醇(CHOL)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)、磷酸肌酸激酶(CK)、白细胞计数(WBC)、中性粒细胞(GR%)、淋巴细胞(LY%)、单核细胞(MO%)、CD3、CD4、CD8、CD4/CD8和丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)等各项指标。结果:和正常动物对照组比较,S180荷瘤模型小鼠血生化指标中TP、ALB、GLB、ALT、AST、GLU、CR、LDH,白细胞计数和分类中的GR%、LY%、MO%,T细胞亚群CD3、CD4、CD8、CD4/CD8,以及混合功能氧化酶MDA、CAT、SOD等指标均出现不同程度改变,且具有统计学差异。结论:小鼠血生化指标中TP、ALB、GLB、ALT、AST、GLU、CR、LDH,白细胞分类中WBC、GR%、LY%、MO%,T细胞亚群CD3、CD4、CD8、CD4/CD8,以及混合功能氧化酶MDA、CAT、SOD等指标可作为动物抗肿瘤试验效果评价中的特异性或非特异性指标进行选择。     

Effect of cold water immersion on oedema formation in the scalded mouse ear

One ear of adult white NMRI hairy mice was first scalded and then immersed in cold water in order to study the effect of cooling on oedema formation. Oedema was determined by wet-dry weight measurements of punch biopsies from the ear and expressed as an increase in tissue water content. The temperature of the cold water was 8 °C and periods of cooling from 15 to 240 minutes were used. The contralateral ear and the quadriceps muscle were used to study distant effects of scalding and cooling.After scalding, significant oedema was found in the burned ear. Cooling for 30 minutes gave a significant reduction of oedema in this model. Shorter or longer periods of cooling did not reduce oedema significantly. Cooling did not influence the oedema formation of the contralateral (unburned ear). Neither scalding nor cooling changed the water content of the muscle.     

Histochemical fibre-type profile in the human masseter muscle

A histochemical characterization of the masseter muscle was performed on biopsy samples of dentate subjects with normal occlusion. There was a continuum of ranges of oxidative and glycolytic capacities of the masseter muscle fibres. Besides the lightly-stained type I and the darkly-stained type II fibres, fibres with intermediate staining reactions for standard ATPase at pH 9.4, IM fibres, were seen in all biopsy samples. IM fibres had some staining characteristics in common with type I, i.e. the reaction for NADH-TR and for ATPase after preincubation at pH 4.6 and 4.2. Like type II fibres they showed strong reaction for menadione-linked alpha-glycerophosphate dehydrogenase and for phosphorylase. The ATPase reaction after preincubation at pH 4.6 did not generally reveal subtypes of type II. It is concluded that the masseter muscle in normal human subjects has a very special fibre composition, with ATPase-IM fibres being a part of the normal fibre population.     

Heat production by adipocytes from obese subjects before and after weight reduction

Microcalorimetry has been employed to measure the heart production by adipocytes obtained by percutaneous biopsy from lean subjects and from obese subjects before and after weight reduction. Cellular heat production was significantly lower in obese than in lean subjects. After weight reduction cellular heat production increased in fat cells from the obese subjects but was still significantly lower than in cells from control subjects. A number of variables reflecting uptake and mobilization of depot fat have measured and correlated to the heat production values in the obese subjects. The findings are consistent with the view that a decreased total metabolic activity might contribute to the development or perpetuation of obesity.