仙草多糖对大鼠肝匀浆脂质过氧化的实验研究

目的 研究仙草多糖对大鼠肝匀浆脂质过氧化的影响，为仙草的保健功能提供实验依据。方法 以大鼠肝匀浆为体外实验体系，以H2O2为氧化损伤因素，应用TBA微量测定法检测仙草多糖的干预对大鼠肝匀浆脂质过氧化产物丙二醛（malondialdehyde,MDA)含量的影响。结果 仙草多糖对H2O2所致大鼠肝匀浆MDA的生成具有明显的抑制作用（P＜0．01），并呈现出一定的剂量－效应关系。结论 仙草糖具有显著的抗脂质过氧化作用。     

人参培养细胞中粗多糖的提取分离及其理化特性

人参(Panax ginseng)是我国传统的珍贵药材,利用人参细胞培养方法产生人参皂甙类有用成分已有不少报道。现代医药学研究表明,人参多糖有较强的药理活性,如刺     

岩舒联合斯奇康治疗恶性胸腹水疗效观察

目的:观察岩舒联合斯奇康治疗恶性胸腹水疗效。方法:将44例恶性胸腹水患者随机分为两组,试验组24例抽取胸腹水后腔内灌注岩舒和斯奇康:岩舒胸腔每次灌注20ml,腹腔每次灌注40ml;斯奇康胸腹腔灌注均为每次18ml,每周1次,连续2～4周。对照组20例采用单药顺铂60mg胸腔内灌注,100mg腹腔内灌注。结果:试验组16例恶性胸水中,有效率(CR PR)87．5%,8例恶性腹水中,有效率75．0%,总有效率83．3%(20/24);试验组24例中,生活质量提高14例,稳定8例,降低2例。对照组15例恶性胸水中,有效率53．3%,5例恶性腹水中,有效率40．0%,总有效率50．0%(10/20);对照组20例中,生活质量提高5例,稳定6例,降低9例。两组比较P＜0．05,试验组优于对照组,无明显不良反应。结论:岩舒联合斯奇康治疗恶性胸腹水疗效可靠,生活质量提高,无明显不良反应,尤其适合治疗晚期不能耐受腔内化疗的患者。     

蚕蛹多糖对小鼠免疫功能的影响

目的 研究蚕蛹多糖对小鼠免疫功能的影响。方法 测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响。结果 蚕蛹多糖明显增强B淋巴细胞和T淋巴细胞的增殖，促进小鼠巨噬细胞 的吞噬活力和溶血素抗体生成。结论 蚕蛹多糖明显增强小鼠的细胞免疫和体液免疫功能。     

Electrospinning of polysaccharides for regenerative medicine

Electrospinning techniques enable the production of continuous fibers with dimensions on the scale of nanometers from a wide range of natural and synthetic polymers. The number of recent studies regarding electrospun polysaccharides and their derivatives, which are potentially useful for regenerative medicine, is increasing dramatically. However, difficulties regarding the processibility of the polysaccharides (e.g., poor solubility and high surface tension) have limited their application. In this review, we summarize the characteristics of various polysaccharides such as alginate, cellulose, chitin, chitosan, hyaluronic acid, starch, dextran, and heparin, which are either currently being used or have potential to be used for electrospinning. The recent progress of nanofiber matrices electrospun from polysaccharides and their biomedical applications in tissue engineering, wound dressings, drug delivery, and enzyme immobilization are discussed.     

猪苓多糖和氢化考的松对小鼠腹腔巨噬细胞的作用——细胞化学研究

小鼠注射猪苓多糖和氢化考的松7天后,取其腹腔巨噬细胞,进行细胞化学的定性及定位;并用 MPV-2型显微分光光度计进行定量研究。发现应用氢化考的松后,细胞内的 AcPase、ATPase 和 ANAE 活性下降,多糖含量减少。注射猪苓多糖以后,ATPase、AcPase 和 ANAE 活性增强,多糖含量显著增多。猪苓多糖和氢化考的松同时注射,酶活性及多糖含量与对照组无明显区别。     

Impact of reattaching various Aeromonas salmonicida A-layer proteins on vaccine efficacy in Atlantic cod (Gadus morhua)

Atypical Aeromonas salmonicida causes atypical furunculosis in a whole range of farmed fish species. The bacterium comprises a heterogeneous group differing in surface components such as the A-layer protein and O-chain polysaccharide structures. Previously, the A-layer protein was shown to contribute to protective immunity as a vaccine with A. salmonicida cells with reattached A-layer protein protecting significantly better than the corresponding A-layer deficient bacteria used in the Atlantic cod (Gadus morhua) vaccine. In the present study, genetically different A. salmonicida A-proteins, either as preformed A-layer sheets from culture supernatants or as purified preparations, were shown to attach to A-layer deficient isolates with a different O-chain structure. Only vaccines containing A. salmonicida cells with reattached A-protein genetically homologous to the challenge isolate, elicited protection comparable to that of the homologous vaccine.     

树舌多糖对心血管系统作用的研究

本文研究了树舌多糖(GAPS)对心血管系统的作用,结果表明:对离体蛙心,GAPS有明显的抑制心肌收缩力和减慢心率作用;对家兔血压有降压作用,此作用可能是其对心脏抑制作用的结果;对垂体后叶素引起家兔心电图中T波抬高有明显的拮抗作用,但对正常家兔心电图无明显影响。从而说明GAPS有增加心肌供血供氧和降低心肌耗氧的作用,可作为治疗冠心病药物试用于临床。     

云芝多糖及银耳孢子多糖的抗突变作用

云芝多糖与银耳孢子多糖均属于真菌多糖,药理作用有某些类似。日本从Coriolusversicolor(Fr)Quel中提取的云芝多糖(PS-K)具有抗肿瘤及加强免疫的作用。我院从Polystictus versicolor(L.)Fr中提取的云芝多糖(PV-1),对免疫功能具有增强作用,启东肝癌防治研究所用我院试制的云芝糖浆,治疗甲胎蛋白(APP)低浓度持续阳性病人,取得显著疗效,AFP转阴率明显大于对照组,认为对肝癌的发生、发展可能具有阻断或延缓作用。银耳(Tremella fuciformis Berck)为著名补养中药,据报道,银耳孢子多糖对免疫功能有增强作用,含多糖的银耳制剂有抗放射作用,并在抗放射实验中发现可降低受照射动物淋巴细胞染色体的畸变率,对遗传物质似有保护作用。本文以小鼠骨髓微核试验,就此三个多糖对细胞染色体损伤的影响作了一些实验研究。     

Biofilm: the microbial “bunker” for intravascular catheter-related infection

Catheter-related infection in cancer patients remains an important health-care problem with major financial implications. During the last few years a better understanding of the pathogenesis of catheter-related infections and the interaction between microorganisms and catheter surfaces has emerged. Recently the influence of biofilm formation in catheter-related infections has been established. The development of biofilm by the colonizing microbes permits attachment of the organisms to the vascular access device and confers resistance to antibiotics and host defense mechanisms. Strategies to overcome the development of biofilm are being developed to prevent catheter- and other medical device-related infections.