分割放疗对鼻咽癌组织细胞增殖凋亡的影响

目的：探讨常规分割放疗和超分割放疗对鼻咽癌组织细胞增殖凋亡的影响。方法：采用ＴｄＴ酶介导的生物素化ｄｕｔｐ缺口末端标记技术（ＴＵＮＥＬ）和免疫组织化学Ｓ－Ｐ法,分别检测６０例鼻咽癌患者在放疗第２周和第４周时细胞凋亡率（ａｐｏｐｔｏｓｉｓ ｒａｔｅ,ＡＲ）和增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｏｎ ｃｅｌｌ ｎｈｕｃｌｅａｒ ａｎｔｉｇｅｎ,ＰＣＮＡ）的表达。结果：常规分割放疗组放疗第４周后的ＡＲ     

鼻咽癌患者EB病毒与乙肝病毒双重感染的观察

目的：探讨鼻咽癌患者ＥＢ病毒和乙肝病毒感染的关系。方法检测５０５１健康康者和１２５例鼻咽癌患者的血清ＥＢＶ－ＩｇＡ／ＶＣＡ和ＨＢｓＡｇ。结果健康人群的ＥＢＶ－ＩｇＡ／ＶＣＡ和ＨＢｓＡｇ的阳性率分别为５．４％和１１．３％;健康人群ＥＢ病毒感染者的ＨＢｓＡｇ阳性率为９．７％;鼻咽癌患者的ＥＢＶ－ＩｇＡ／ＶＣＡ和ＨＢｓＡｇ的阳性率分别为９７．６％、２５．６％。鼻咽癌ＥＢ病毒、乙肝病毒双重感染率明显高于正     

鼻咽癌高癌家系成员EB病毒VCA—IgA抗体水平分析

目的：探讨鼻咽癌高癌家系成员的ＥＢ病毒ＶＣＡ－ＩｇＡ抗体水平。方法：用免疫酶法分别检测高癌家系成员共４１６人和自然人群８７４人的ＶＣＡ－ＩｇＡ抗体。结果：高癌家系成员的ＶＣＡ－ＩｇＡ阳性率为２６．４４％（１１０／４１６）,明显高于自然人群的４．２３％（３７／８７４）。结论：鼻咽癌高癌家系成员中的高ＥＢ病毒感染率与鼻咽癌的高发病率相关。     

XRCC1单核苷酸多态性与鼻咽癌铂类化疗敏感度的相关性

目的 探讨鼻咽癌铂类化疗敏感度与X射线交错互补修复基因1 codon194和codon399单核苷酸多态性的相关性。方法 收集广西医科大学第四附属医院2012年9月1日至2013年12月31日鼻咽部肿物活检确诊为鼻咽癌患者资料,采用限制性片段长度多态性聚合酶链反应技术检测鼻咽癌患者外周血DNA XRCC1 codon194和codon399单核苷酸多态性。顺铂+氟尿嘧啶方案诱导化疗2周期后复查MRI,按照RECIST 1.1标准评价其化疗敏感度,分析单核苷酸多态性（Single nucleotide polymorphism,SNP）与化疗敏感度的关系。结果 XRCC1 codon399 Gln/Gln基因型携带者化疗敏感度为Arg/Arg基因型携带者的3.500倍（P＜0.05）。XRCC1 codon399不含Arg基因型（即Gln/Gln）携带者化疗敏感度为含Arg基因型（Arg/Arg 和 Arg/Gln）携带者的3.274倍,（P＜0.05）。携带XRCC1 codon194各基因型患者化疗敏感度之间差异无明显统计学意义（P＞0.05）。结论 XRCC1 codon399 单核苷酸多态性有可能成为鼻咽癌铂类化疗敏感度的预测因子。     

紫杉醇联合四君子汤对鼻咽癌荷瘤鼠的疗效研究

目的研究紫杉醇联合四君子汤对鼻咽癌模型小鼠的治疗效果。方法测量肿瘤体积,血细胞计数;放射免疫分析法测定血清介质含量。结果紫杉醇联合四君子汤具有明显的抑制肿瘤生长的作用;与单用紫杉醇组比较,四君子汤可以增强紫杉醇的抗肿瘤作用,并降低紫杉醇免疫抑制的副作用。结论紫杉醇与四君子汤合用可增强抗肿瘤作用,且和增强机体免疫调节功能有关。     

Adenovirus-mediated transfer of tris-shRNAs induced apoptosis of nasopharyngeal carcinoma cell in vitro and in vivo

RNA interference (RNAi) is an evolutionary conserved mechanism for specific gene silencing. There are currently numerous cancer therapy clinical trials based on RNAi technology. Using an adenoviral system as a delivery mediator of RNAi, we investigated the therapeutic effects of targeting three genes simultaneously in vitro and in vivo. In this study, we constructed an recombinant adenoviral shRNA expression system as Adv-pEGFP-shVEGF-shTERT-shBcl-xl for multi-genes silencing. Our results showed that the adenoviral vector can achieve above 90% of transfection efficiency and induced obvious apoptosis in CNE-2 cell both in vitro and in vivo compared with targeting the TERT alone or controlled group.     

Distinct patterns of neuronal inputs and outputs of the juxtaparaventricular and suprafornical regions of the lateral hypothalamic area in the male rat

We have analyzed at high resolution the neuroanatomical connections of the juxtaparaventricular region of the lateral hypothalamic area (LHAjp); as a control and in comparison to this, we also performed a preliminary analysis of a nearby LHA region that is dorsal to the fornix, namely the LHA suprafornical region (LHAs). The connections of these LHA regions were revealed with a coinjection tract-tracing technique involving a retrograde (cholera toxin B subunit) and anterograde (Phaseolus vulgaris leucoagglutinin) tracer. The LHAjp and LHAs together connect with almost every major division of the cerebrum and cerebrospinal trunk, but their connection profiles are markedly different and distinct. In simple terms, the connections of the LHAjp indicate a possible primary role in the modulation of defensive behavior; for the LHAs, a role in the modulation of ingestive behavior is suggested. However, the relation of the LHAjp and LHAs to potential modulation of these behaviors, as indicated by their neuroanatomical connections, appears to be highly integrative as it includes each of the major functional divisions of the nervous system that together determine behavior, i.e., cognitive, state, sensory, and motor. Furthermore, although a primary role is indicated for each region with respect to a particular mode of behavior, intermode modulation of behavior is also indicated. In summary, the extrinsic connections of the LHAjp and LHAs (so far as we have described them) suggest that these regions have a profoundly integrative role in which they may participate in the orchestrated modulation of elaborate behavioral repertoires.     

LTBP-2 confers pleiotropic suppression and promotes dormancy in a growth factor permissive microenvironment in nasopharyngeal carcinoma

This study identified LTBP-2 as a pleiotropic tumor suppressor in nasopharyngeal carcinoma, which safeguards against critical malignant behaviors of tumor cells. LTBP-2 expression was significantly decreased or lost in up to 100% of NPC cell lines (7/7) and 80% of biopsies (24/30). Promoter hypermethylation was found to be involved in LTBP-2 silencing. Using a tetracycline-regulated inducible expression system, we unveiled functional roles of LTBP-2 in suppressing colony formation, anchorage-independent growth, cell migration, angiogenesis, VEGF secretion, and tumorigenicity. Three-dimensional culture studies suggested the involvement of LTBP-2 in maintenance of tumor cell dormancy in a growth factor favorable microenvironment.     

Cone beam computed tomographic analyses of alveolar bone anatomy at the maxillary anterior region in Chinese adults

To provide an anatomical basis for clinical implant esthetics,we evaluated the morphology of the nasopalatine canal（NPC） and analyzed labial and interproximal bone anatomy at the maxillary anterior region.We sought to investigate the effect of maxillary protrusion and tooth labiolingual inclination on labial bone anatomy in Chinese adults.Three dimensional（3D） images were reconstructed using cone-beam computed tomography（CBCT） images from 80 Chinese subjects and by SimPlant 11.04.The dimensions of the NPC,the thickness and profile of the labial bone,the width and height of the interproximal bone,angle sella-nasion-subspinale（SNA） and angle upper central incisor-nasion,subspinale（U1-NA） were measured.The incisive foramen of the NPC was markedly wider than its nasal foramen.The dimension of its labial bone wall demonstrated an increasing width from the crestal to apical measurements.The labial bone at the maxillary anterior region was rather thin,especially at 3 mm below the cemento-enamel junction（CEJ） and the mid-root level;the profile of the labial bone was more curved at the central incisor,and the interproximal bone became wider and shorter posteriorly.There were significant relationships between maxillary protrusion and labial bone profile,tooth labiolingual inclination and labial bone thickness（P 〈 0.02）.To achieve optimal esthetic outcome of implant,bone augmentation is necessary at the maxillary anterior region.For immediate or early placement at the maxillary anterior region,the implant should be located palatally to reduce labial bone resorption and marginal recession;its apex should be angulated palatally to avoid labial perforation at the apical region.To protect the NPC,implants at the central incisor region should be placed away from NPC.