注射器不同位置放置时胰岛素微量注射过程中的浓度测定

目的 探讨注射器不同位置放置时微量注射胰岛素(Regular Insulin,RI)稀释液过程中的浓度变化,以指导三升袋输注过程中合理使用RI.方法 20 ml注射器抽取RU稀释液20 ml,其中含19.5 ml的5%葡萄糖,0.5 ml的砌20 U.根据注射器水平和垂直放置以及乳头位置的不同将试验分为3组,泵速4 U/h,分别于注射前、注射后1 h、2 h、3 h、4 h分别取样1 ml,使用电化学发光分析仪测定RI浓度.结果 微量注射泵在3种位置5个时间段注射RI溶液的浓度变化值为:33.3±2.7mU/L,35.0±3.5 mU/L,38.3±4.6 mU/L,38.4±3.6 mU/L,36.1±3.9 mU/L,5组之间方差分析无统计学差异(P>0.05).结论 用微量注射泵注射RI稀释液的过程中浓度较稳定,配合3 L袋输液能够减少m浓度变化对于血糖的影响.     

HPLC法测定复方丹参微丸中丹酚酸B的含量

目的：用高效液相色谱法测定复方丹参微丸中丹酚酸B的含量。方法以乙腈-甲醇-甲酸-水（10∶30∶1∶59）为流动相,色谱柱 Diamon-silTM C18（5μm,4.6＆#215;250mm）,波长286nm、柱温40℃、流速1.0mL＆#183; min -1为色谱条件,对复方丹参微丸中丹酚酸 B含量进行分析。结果丹酚酸B在59.5～1487.5 ng范围内成良好的线性关系（ r＝0.99995）。结论本法简便、准确、重现性好。     

两种不同方法分离宫腔粘连的临床观察

目的探讨两种不同方法分离宫腔粘连的预后。方法将中重度宫腔粘连患者随机分为研究组（32例）和对照组（30例）,研究组采用宫腔镜下微型剪刀切除术,对照组采用宫腔镜下电切术。两组术后均予上环术加透明质酸钠宫腔内注射及雌、孕激素人工周期治疗3个月;术后1、3个月行宫腔镜检查;术后6、18个月各进行随访1次。结果研究组与对照组术后3个月宫腔形态恢复总有效率分别为90．6％（29／32）、93．3％（28／30）;月经改善总有效率分别为81．3％（26／32）、80．0％（24／30）;宫腔粘连的复发率分别为15．6％（5／32）、13．3％（4／30）;妊娠率分别为34．6％（9／26）、27．3％（6／22）;两组比较差异无统计学意义（P〉0．05）。两组住院天数、手术费用、总费用比较差异有统计学意义（P〈0．001）。两组均无明显的药物不良反应及手术并发症。结论两种不同方法分离宫腔粘连疗效无统计学差异,宫腔镜下微型剪刀切除术是一种值得临床推广的治疗方法。     

Molecular pathways of liver regeneration: A comprehensive review

The liver is a unique parenchymal organ with a regenerative capacity allowing it to restore up to 70% of its volume. Although knowledge of this phenomenon dates back to Greek mythology(the story of Prometheus), many aspects of liver regeneration are still not understood. A variety of different factors, including inflammatory cytokines, growth factors, and bile acids, promote liver regeneration and control the final size of the organ during typical regeneration, which is performed by mature hepatocytes, and during alternative regeneration, which is performed by recently identified resident stem cells called "hepatic progenitor cells". Hepatic progenitor cells drive liver regeneration when hepatocytes are unable to restore the liver mass, such as in cases of chronic injury or excessive acute injury. In liver maintenance, the body mass ratio is essential for homeostasis because the liver has numerous functions; therefore, a greater understanding of this process will lead to better control of liver injuries, improved transplantation of small grafts and the discovery of new methods for the treatment of liver diseases. The current review sheds light on the key molecular pathways and cells involved in typical and progenitor-dependent liver mass regeneration after various acute or chronic injuries. Subsequent studies and a better understanding of liver regeneration will lead to the development of new therapeutic methods for liver diseases.     

Involvement of N-cadherin/β-catenin interaction in the micro/nanotopography induced indirect mechanotransduction

Topographical modification at micro- and nanoscale is widely applied to enhance the tissue integration properties of biomaterials, but the underlying molecular mechanism is poorly understood. The biomaterial topography modulates cell functions via mechanotransduction of direct and indirect. We propose that N-cadherin may play a role in the topographically induced indirect mechanotransduction by regulating the β-catenin signaling. For confirmation, the cell functions, N-cadherin expression and β-catenin signaling activation of osteoblasts on titanium (Ti) surfaces with micro- or/and nanotopography are systemically compared with naive and N-cadherin down-regulating MC3T3-E1 cells. We find that the N-cadherin expression is reversely related to the intracellular β-catenin signaling and the N-cadherin/β-catenin signaling is modulated differentially by the micro- and nanotopography. The nanotopography significantly up-regulates the N-cadherin expression leading to lower β-catenin signaling activity and consequently depressed differentiation, whereas the microtopography down-regulates the N-cadherin expression resulting in enhanced β-catenin signaling and thus osteoblast differentiation. Artificial down-regulation of the N-cadherin expression can significantly up-regulate the β-catenin signaling and consequently enhance the osteoblast differentiation on all the Ti surfaces. The study for the first time clarifies the involvement of the N-cadherin/β-catenin interaction in the micro/nanotopography induced indirect mechanotransduction and provides a potentially new approach for biomaterial modification and biofunctionalization by down-regulating the cell N-cadherin expression to achieve improved clinical performance.     

The Muscle Sensor for on-site neuroscience lectures to pave the way for a better understanding of brain–machine-interface research

Neuroscience is an expanding field of science to investigate enigmas of brain and human body function. However, the majority of the public have never had the chance to learn the basics of neuroscience and new knowledge from advanced neuroscience research through hands-on experience. Here, we report that we produced the Muscle Sensor, a simplified electromyography, to promote educational understanding in neuroscience. The Muscle Sensor can detect myoelectric potentials which are filtered and processed as 3-V pulse signals to shine a light bulb and emit beep sounds. With this educational tool, we delivered “On-Site Neuroscience Lectures” in Japanese junior-high schools to facilitate hands-on experience of neuroscientific electrophysiology and to connect their text-book knowledge to advanced neuroscience researches. On-site neuroscience lectures with the Muscle Sensor pave the way for a better understanding of the basics of neuroscience and the latest topics such as how brain–machine-interface technology could help patients with disabilities such as spinal cord injuries.     

六西格玛在手术室显微器械管理中的应用

目的探讨六西格玛质量管理理念与方法对规范手术室显微器械的管理,提高手术室护理质量的作用。方法运用六西格玛的定义-测量-分析-改进-控制5个步骤管理模式对手术室显微器械实施管理,并与实施六西格玛质量管理前进行比较。结果改进措施后显微器械术前准备缺陷率明显低于改进措施前(P0.05),器械清洁度明显提高、护士责任意识增强、医生的满意度提升(P0.05)。结论运用科学管理提高效率,减少失误,使显微器械的准备过程达到最优状态。     

载药聚酯超声微泡造影剂制备及应用研究

超声造影剂在临床诊断和鉴别中发挥着极为重要的作用.概述了超声造影剂的特点和发展过程,用W/O/W双乳液溶剂挥发法制备PLGE载药超声微泡造影剂,体外超声显影效果良好.载药聚酯超声微泡造影剂在药物治疗和超声显影领域有良好的应用前景.