PATZ1 acts as a tumor suppressor in thyroid cancer via targeting p53-dependent genes involved in EMT and cell migration

PATZ1, a POZ-Zinc finger protein, is emerging as an important regulator of development and cancer, but its cancer-related function as oncogene or tumor-suppressor is still debated. Here, we investigated its possible role in thyroid carcinogenesis. We demonstrated PATZ1 is down-regulated in thyroid carcinomas compared to normal thyroid tissues, with an inverse correlation to the degree of cell differentiation. In fact, PATZ1 expression was significantly further down-regulated in poorly differentiated and anaplastic thyroid cancers compared to the papillary histotype, and it resulted increasingly delocalized from the nucleus to the cytoplasm proceeding from differentiated to undifferentiated thyroid carcinomas. Restoration of PATZ1 expression in three thyroid cancer-derived cell lines, all characterized by fully dedifferentiated cells, significantly inhibited their malignant behaviors, including in vitro proliferation, anchorage-independent growth, migration and invasion, as well as in vivo tumor growth. Consistent with recent studies showing a role for PATZ1 in the p53 pathway, we showed that ectopic expression of PATZ1 in thyroid cancer cells activates p53-dependent pathways opposing epithelial-mesenchymal transition and cell migration to prevent invasiveness. These results provide insights into a potential tumor-suppressor role of PATZ1 in thyroid cancer progression, and thus may have potential clinical relevance for the prognosis and therapy of thyroid cancer.     

低氧对肝癌细胞上皮间质转化及侵袭转移能力的影响

目的:观察低氧环境下肝癌细胞上皮-间质转化(EMT)情况和侵袭转移能力的改变。方法:选取2种具有不同侵袭转移能力的人肝癌细胞株SMMC-7721和HepG2,通过1%O2低氧培养建立人肝癌细胞物理缺氧模型。动态观察2种细胞在低氧环境中的形态学改变;应用Westernblot方法检测上皮细胞标志蛋白E-cadherin和间质细胞标志蛋白vimentin表达的变化;应用Transwell小室检测肝癌细胞迁移、侵袭能力。结果:低氧环境中,该2种肝癌细胞均由原来的紧密排列上午上皮样状态转为较松散的纺锤体样改变;Western blot检测显示,2种肝癌细胞株的E-cadherin表达量明显下降(均P<0.01),而vimentin表达量明显升高(均P<0.01);Transwell迁移及侵袭实验显示,2种肝癌细胞的迁移及侵袭能力明显增强(均P<0.01);SMMC-7721细胞的上述变化均较HepG2细胞明显(均P<0.01)。结论:低氧环境可诱导肝癌细胞发生EMT,并增强肝癌细胞的迁移和侵袭能力。     

BOP1与肿瘤关系的研究进展

增殖阻断蛋白1(BOP1)是一种核蛋白。它参与核糖体的生物合成,进而影响蛋白质的合成。近来研究表明,BOP1在某些肿瘤中表达增高,并在上皮间质转化(EMT)、肿瘤发生发展、侵袭转移方面发挥关键作用。BOP1在肿瘤中的具体作用机制尚不十分明了。笔者就其生物学特性及与肿瘤的关系的研究进展作一综述。     

生脉散体外对低密度脂蛋白氧化修饰的保护作用

本文以低密度脂蛋白氧化修饰为模型，以硫代巴比妥酸反应物质生成量、维生素Ｅ和荧光物质含量变化为指标，研究了生脉散对Ｃｕ２＋和Ｆｅ２＋诱导低密度脂蛋白氧化修饰的抑制作用。结果说明，生脉散能明显地抑制Ｃｕ２＋和Ｆｅ２＋诱导低密度脂蛋白氧化修饰，对这两种过渡金属诱导低密度脂蛋白氧化修饰的抑制速率和程度相似。生脉散抑制低密度脂蛋白氧化修饰的机制主要是清除脂自由基。     

应用姑息治疗在妇科恶性肿瘤患者临终时作用的研究

目的 探讨姑息治疗在妇科恶性肿瘤患者临终时的作用效果.方法 选取油田总院集团下属所有医院确诊的妇科恶性肿瘤患者,疾病已处于晚期不可治阶段,自愿参与本次研究患者30例,预计生存期6个月的患者,作为观察组,此组接受了姑息治疗、临终关怀护理;将已经去世的2004至2009年的龙南医院妇科恶性肿瘤患者30例全部住院病例及当年调查表,作为对照组,调查收集患者疼痛、疲乏、情绪、恶心呕吐情况.结果 本次调查观察组各方面均比对照组有明显效果.结论临终关怀及姑息治疗对帮助妇科肿瘤患者提高生活质量,对待死亡提供了良好的过渡.     

Changes in Gross Motor Function and Health-Related Quality of Life in Adults With Cerebral Palsy: An 8-Year Follow-Up Study

Objective

To describe changes in gross motor function and health-related quality of life (HRQOL) in adults with cerebral palsy (CP).

Design

An 8-year follow-up survey.

Setting

Participants who completed the baseline survey in 2003 were invited.

Participants

The sample of adults with CP (N=54; response rate=37%) included a “younger group” (group 1; n=31; age, 23–27y; 15 women) and an “older group” (group 2; n=23; age, 33–42y; 10 women).

Interventions

Not applicable.

Main Outcome Measures

The Gross Motor Function Classification System (GMFCS), Self-Rated Health (SRH), the Health Utility Index Mark III (HUI₃), and the Assessment of Quality of Life (AQoL).

Results

Eight years after the initial survey, 27% of the participants in the combined group had deteriorations on the GMFCS, 52% on the SRH, 44% on the HUI₃, and 25% on the AQoL. Members of group 1 reported stable scores as they made the transition to adulthood, while many of the group 2 members experienced declines, with relative risk of 1.47 (95% confidence interval [CI], 0.16–2.24) on the GMFCS, 1.36 (95% CI, 0.83–2.23) on the SRH, 1.19 (95% CI, 0.66–2.15) on the HUI₃, and 3.17 (95% CI, 1.12–9.00) on the AQoL.

Conclusions

Although much attention has focused on the transitions of persons with CP during their late teens and early 20s, this research found that deteriorations in the GMFCS levels and the HRQOL were most evident in adults in their late 20s and 30s. More detailed longitudinal studies are required to evaluate the longer-term health outcomes among persons with CP into their 30s and beyond.     

慢性病青少年及照护者双视角下成长过渡期准备的纵向研究

目的 探究慢性病青少年及其照护者双视角下,成长过渡期准备水平的变化过程及对彼此生活质量的影响,明确家庭照护适应度在此过程中的调控作用。方法 采用前瞻性研究设计,便利选取2019年3月—9月在上海市2所儿童专科医院的肾病、风湿病和内分泌病房住院的201例慢性病青少年及其照护者作为调查对象。在住院期间、出院后1年和出院后2年,采用一般资料调查问卷、中文版家庭管理测量量表、中文版成长过渡期准备问卷、中文版儿童生存质量测定量表及中文版儿童生命质量家庭影响模块进行调查。采用纵向主-客体互依模型,分析成长过渡期准备水平对慢性病青少年及其照护者生活质量的影响。结果 重复测量方差分析结果显示,慢性病青少年及其照护者视角下的成长过渡期准备水平逐年增高（均P<0.001）。相关分析结果显示,出院后2年慢性病青少年及其照护者视角下的成长过渡期准备水平与彼此生活质量呈正相关（P<0.001）。主-客体互依模型分析结果显示,慢性病青少年视角下的成长过渡期准备水平对其生活质量有正向主体效应（P<0.05）;照护者视角下的成长过渡期准备水平对其自身和青少年的生活质量有正向主体、客体效应（P<0.05）;家庭照护适应度对青少年及其照护者视角下的成长过渡期准备水平均有正向影响（P<0.05）。结论 慢性病青少年及其照护者双视角下的成长过渡期准备水平能正向预测彼此的生活质量。医护人员应在不同阶段提供有针对性的家庭护理,提高慢性病青少年的自我管理能力,进而提升家庭整体生活质量。     

Transition and adaptation to adoptive motherhood

This article reviews research on the adaptation and transition to motherhood for women who adopt children, with the goal of identifying implications for clinical practice. Relevant articles focusing on the early transitions to adoptive motherhood were identified by searching electronic databases. Evidence on the preadoptive and early postadoptive periods was evaluated. The concepts identified by researchers and the women's feelings they described are discussed. Overall, the process of adaptation to motherhood was very similar, but adoptive parents reported unique emotions and concerns in the transition to parenthood. By understanding these findings, nurses can help support adoptive mothers' continued success in navigating stressful and uncertain paths to motherhood.