重组质粒pMG611中K99和F41抗原基因在E．coli中表达条件的研究

本文通过ELISA方法测定了重组质粒pMG611在不同宿主和培养条件下，K99和F41菌毛抗原的表达水平。研究结果表明重组质粒pMG611在HB101和RRI宿主菌中较在C600宿主菌中表达更多的K99和F41菌毛抗原。实验中还探讨了培养基、温度和丙氨酸对两种菌毛抗原表达的影响。     

对超期妊娠实施产科干预的临床总结

目的:探讨对超期妊娠实施产科干预对分娩方式等方面的影响。方法:选择无任何合并症及并发症的超期妊娠294例,就其宫颈评分、对静滴缩宫素引产的依从性、最终分娩方式、新生儿体重进行回顾性总结分析。结果:294例超期妊娠中,愿意实施缩宫素引产者仅占30.6%,引产成功127例,其余均实施了剖宫产,新生儿体重在3500g以上者177例。结论:超期妊娠者胎儿巨大儿发生较多,对静滴缩宫素引产的依从性较差,如进行适当的产科干预,可降低剖宫产率。     

Extrapolation: Experience gained from original biologics

Biologicals undergo modifications throughout their commercial lifecycle. Major changes can unintentionally magnify their inherent physicochemical variability. Although trials comparing the pre- and the post-change versions have been requested occasionally, analytical comparison is the most sensitive approach to anticipating clinical equivalence. Therefore, it may be concluded, by means of ‘extrapolation’, that non-identical versions of a given biologic will behave equally in all indications. Despite the lessons learned with original biologics, there are still controversies around the approval of biosimilars through extrapolation. Here, a comprehensive analysis of scattered information allows for an account of cases of original biologic versions approved in some indications with no patient trials involved. Healthcare professionals can be reassured that inasmuch as extrapolation has proven valid for new versions of original biologics, the same holds for biosimilars.     

HIV-1 p24和gp41融合基因表达载体的构建及融合蛋白的表达纯化

目的用基因重组技术将HIV-1p24基因以及gp41基因具有抗原线性中和表位的部分重组连接，构建重组质粒，并在大肠埃希菌中高效表达融合蛋白。方法设计带有酶切位点的引物，分别PCR扩增p24和gp41两个基因，将它们分别连接到pMD18-T载体中，测序验证后，挑选出含有目的基因的正确克隆。将p24片段酶切后连接到gp41基因所在的pMD18T载体中，再将连接后的两个基因酶切，重新连接到pET21a表达载体中。将表达载体转染大肠埃希菌诱导表达，经Western-blot验证表达正确。结果融合蛋白p24-gp41在大肠埃希菌中高效表达。结论融合蛋白p24-gp41可以在pET21a表达载体中高效表达。     

Effectiveness of various hip preservation treatments for non-traumatic osteonecrosis of the femoral head: A network meta-analysis of randomized controlled trials

Background

Non-traumatic osteonecrosis of the femoral head (ONFH) is a refractory osteonecrosis disease caused by an abnormal blood supply to bone tissue. However, therapeutic hip preservation strategies are diverse, and the therapeutic outcomes are not ideal.

Human leukocyte antigen-DR expression in peripheral blood mononuclear cells from healthy donors influenced by the sera of injured patients prone to severe sepsis

Objective To study the influence of sera from severely injured patients on the human leukocyte antigen (HLA)-DR expression of normal peripheral blood mononuclear cells (PBMC).Design In vitro study.Setting University hospital.Patients and participants Sera from 34 patients were obtained within 8 h after trauma. Seventeen of these patients developed posttraumatic sepsis (sepsis group) and 17 recovered without infectious complications. Sera from ten healthy individuals served as controls. Phytohemagglutinin (PHA)-activated PBMC from 44 healthy donors were used to study the effects of a patient's serum.Measurements and results Medium containing 5% of serum from the sepsis group significantly (p<0.05) reduced the HLA-DR expression (channels, mean ± standard error of the mean) on monocytes (patients 883±22, controls 962±15), B (patients 922±14, controls 972±7) and T cells (patients 932±13, controls 968±5) of PHA-activated PBMC. Significantly increased accumulation of TNF on (1.8±0.4% of PBMC) and within T cells (0.98±0.26% of PBMC) was observed by flow cytometry after incubation with medium containing sera of the sepsis group compared with controls (on 0.5±0.1%, within 0.27±0.05% of PBMC). A significant negative correlation between relative cell counts of intracellular TNF-positive T cells with HLA-DR expression was observed for monocytes (r= –0.61), B cells (r= –0.57) and proliferation (r= –0.68) as estimated by ³H-thymidine uptake [patients 139,971±12,844 counts per minute (cpm), controls 198,973±19,347 cpm, p<0.05] in the presence of sera from the sepsis group.Conclusions Reduced cellular immunity and, therefore, immunodeficiency after trauma appears to be caused by soluble factors influencing T cell function in particular.     

大学生体力活动与血瘀质的剂量-反应关系-基于限制性立方样条模型

[目的]体力活动与中医体质的关系日益受到人们的关注,但有关体力活动与血瘀质的关系研究较少,且尚未有研究探索体力活动与血瘀质的剂量-反应关系。探索大学生体力活动与血瘀质的剂量-反应关系,为其科学合理的运动提供建议。[方法]采用立意抽样结合滚雪球抽样法,于2019年2—6月在大学生中施测,回收有效问卷500例,剔除不运动、分析变量缺失值者48例,452例样本纳入分析。以中医体质量表-41条目简短版(CCMQ-41)中的血瘀质亚量表测评大学生的血瘀质水平,国际体力活动问卷-短卷(IPAQ-SF)测评其总体力活动(计算每周高、中强度体力活动和日常步行能量代谢当量之和)。利用多元线性回归分析及限制性立方样条模型拟合多元线性回归模型评估体力活动与血瘀质之间的关系。[结果]452例大学生体力活动平均为每周(2020.39±1852.39)MET-min,血瘀质得分平均为(35.28±19.91)分。控制混杂因素的多元线性回归分析显示,体力活动与血瘀质之间的关系无统计学意义(线性检验:β=-0.079,t=-1.640,P=0.102);控制混杂因素的限制性立方样条拟合多元线性回归模型显示,体力活动与血瘀质存在近似“L”型的曲线(非线性检验:P-nonlinearity<0.01),血瘀质得分最低点的体力活动约为每周3000 MET-min;对于血瘀质人群来说,随着体力活动的增加,血瘀质得分降低,但当体力活动超过每周3000 MET-min,血瘀质得分基本维持在平稳水平。[结论]体力活动与血瘀质存在近似“L”型的曲线,适当增加体力活动有助于降低血瘀质得分,提升健康水平。     

A Study on Nursing Students' Knowledge,Attitude, and Educational Needs for Brain-Death Organ Transplantation and Donation and Intent to Donate Organs

Objective

The purpose of this study was to identify the knowledge, attitude, educational needs, and will of nursing students on organ donation from brain-dead donors.

Left Ventricular Assist Device Implantation in Patients With Optimal and Borderline Echocardiographic Assessment of Right Ventricle Function

Background

Left ventricular assist devices (LVADs) are used for treatment of end-stage heart failure. Outcomes are dependent on right ventricle (RV) function. Prediction of RV function after LVAD implantation is crucial for device selection and patient outcome.The aim of our study was to compare early LVAD course in patients with optimal and borderline echocardiographic parameters of RV function.