甘肃不同地域乌拉尔甘草质量的化学模式评价

目的评价甘肃不同产地乌拉尔甘草的质量,探讨适宜的种植区域。方法采用高效液相色谱法测定甘草酸和甘草苷含量,紫外法测定总黄酮及水溶性浸出物含量,应用主成分分析和聚类分析综合评价甘草的质量。结果由于甘肃生态条件及种植技术等原因,各地乌拉尔甘草质量存在差异,甘草酸含量范围1.82%～8.25%,甘草苷含量范围0.83%～6.09%,其中2批样品甘草酸未达到《中华人民共和国药典》的规定。结论化学模式评价可以较好地评价质量状况,其结果也可用于优选种植适宜区域。     

薄层色谱法定性鉴别缩泉止遗合剂中两主药补骨脂、甘草对照试验研究

[目的]定性鉴别缩泉止遗合剂中两主药补骨脂、甘草。[方法]采用薄层色谱法对制剂中补骨脂、甘草进行了定性鉴别试验。[结果]薄层色谱中样品色谱斑点显色清晰,分离度好。[结论]本方法灵敏、准确,专属性强,重现性好,可作为该制剂的质量控制方法。     

清咽滴丸6种成分提取物体外抗柯萨奇病毒B3、B5的作用研究

目的:研究清咽滴丸6种中药成分提取物体外抗柯萨奇病毒B3、B5(CVB3、CVB5)的作用。方法:以Vero细胞为模型,通过观察药物毒性和病毒致细胞病变效应,用MTT法辅助检测细胞活性情况,检验药物的毒性和药物的抗病毒效应。结果:清咽滴丸的6种主要成分中只有甘草和诃子既可以抑制病毒侵入Vero细胞,又能治疗被CVB3、CVB5感染的Vero细胞。结论:甘草、诃子提取物可保护Vero细胞,抑制病毒在细胞内增殖,并具有一定的抗CVB3、CVB5感染的能力。     

HPLC-MS法鉴定甘草的指纹图谱

目的：对甘草液相色谱指纹图谱主要色谱峰进行定性,了解甘草甲醇-水提取物的化学成份。方法：测定甘草甲醇提取物的HPLC—DAD及HPLC—MS指纹图谱。结果：结合文献对指纹图谱主要色谱峰进行鉴定,推断出17个色谱峰中19个可能的成分。结论：本研究给甘草质量评价等各类研究提供较全面的化学依据。     

分光光度法测定甘草中总黄酮的含量

目的:建立分光光度法测定甘草中总黄酮含量。方法:利用二氢黄酮类在碱性条件下易转化为它的相应异构体-查尔酮类化合物特性,采用分光光度法,以甘草苷为对照品,以10%氢氧化钾为显色剂,在波长为400nm 处对样品中的总黄酮进行含量测定。结果:总黄酮在2.05-41.13μg·mL~(-1)范围内呈良好的线性关系(r=0.9992);该法的平均加样回收率分别为97.6%,RSD 分别为2.0%(n=3)。结论:该方法稳定、简便、快速,适用于甘草中总黄酮含量的测定。     

甘肃产人工种植甘草的质量考察研究

用HPLC法对甘肃产四种不同产地、不同生长年限、不同季节采集的人工种植甘草的甘草酸进行了含量测定和分析,结果显示,采收时间、生长年限、物种不同,甘草酸的含量有明显差异。这一结果为该类药材的质量研究、生产采收、药厂选投料及开发应用等提供了科学依据,同时对该类资源的开发利用也具有积极意义。     

中医方剂—麻杏石甘汤的生物配位—化学的初步研究

本文报道pH电位测定法测出麻黄碱与生石膏中的Cu^2 、Fe^2 、Zn^2 、Mn^2 离子形成的ML及ML2型络合物的稳定常数。滴定曲线显示甘草酸与上述四种金属离子也能分别形成不同稳定程度的络合物，用麻黄碱加甘草酯和上述四种金属离子配制成的模拟麻杏石甘汤和单纯生石膏煎剂与麻性石甘汤一样均有使家兔退热的效果。作者认为应从生物－配位无机化学的多金属－多配体体系的观点去研究某些传统中医药方剂。     

甘草中三种皂甙类成分的高效液相色谱法分离和含量测定

本文报道应用高效液相色谱法对甘草中三种皂甙类成分甘草酸(glycyrrhizic acid,S-Ⅰ),乌拉甘草皂甙甲(uralsaponin A,S-Ⅲ)和乌拉甘草甙乙(uralsaponin B,S-Ⅱ)进行分离和含量测定。采用ODS柱,乙腈—3%乙酸(47:53)为流动相,248 nm为检测波长,三种皂甙分离良好,可在20 min内完成测定。     

90-Day repeated-dose toxicity study of licorice flavonoid oil (LFO) in rats

Licorice flavonoid oil (LFO) is a new functional food ingredient consisting of licorice hydrophobic polyphenols in medium-chain triglycerides (MCT). As part of a safety evaluation, a 90-day oral toxicity study in rats was conducted using an LFO concentrate solution (2.90% glabridin). Male and female animals were assigned to one of 12 groups (10 males or females per group) and received corn oil (negative control), MCT (vehicle control), or 400, 600, 800 or 1600 mg/kg of the LFO concentrate solution. In conclusion, LFO concentrate solution induced an anticoagulation effect in both sexes, although there was a clear sex difference. Based on these findings, it is concluded that the no-observed-adverse-effect level (NOAEL) for the LFO concentrate solution is estimated to be 800 mg/kg/day for female rats, and approximately 400 mg/kg/day for male rats.     

A hidden cause of hypokalemic paralysis in a patient with prostate cancer

Hypokalemic paralysis is a medical emergency due to the risks of cardiac arrhythmia, respiratory failure, and rhabdomyolysis. Besides supplementing patients with KCl to hasten recovery, the astute physician must search for the underlying cause to avoid missing a treatable and curable disorder. We report on an elderly Korean man who presented with marked limb paralysis, myalgias, and mild hypertension. He had prostate cancer treated with orchiectomy and hormone therapy 2 years previously. The major biochemical abnormalities were hypokalemia (K⁺: 1.7 mmol/l) associated with high renal K⁺ wasting and metabolic alkalosis (HCO₃^–: 42.6 mmol/l). Low plasma renin activity, low aldosterone concentration, and normal cortisol concentration pointed to a state of pseudohyperaldosteronism. While reviewing his drug history, the patient revealed he had been consuming eight packs (100 ml/pack) of a Korean herbal tonic daily to treat his prostate cancer for the past 2 months. A significant amount of glycyrrhizic acid (0.23 mg/ml), an active ingredient of licorice, was detected in the tonic. Discontinuation of the herbal tonic along with KCl supplementation achieved recovery in 2 weeks. As many complementary/alternative medicines for cancer contain licorice, this must be kept in mind as a cause of hypokalemia in cancer patients.