Evaluation of wound healing activities of kefir products

Kefirs are natural probiotic compounds with antibacterial and anti-inflammatory properties which were tested in experimental burn injury. Kefir gels were prepared from an extract of continuously cultured kefir grains in MRS Broth medium for 24, 48 and 96h. Similar burn injuries were made on dorsal skin surface of 56 rats. After 24h the wounds were infected with Pseudomonas aeruginosa. The infected rats were divided in to 7 groups of 8 rats each. The base gel, silver sulfadiazine ointment, kefir 24h gel, kefir 48h gel, kefir 96h gel and kefir grains 96h gel were applied twice a day. Burn wound area was measured at baseline, one and two weeks. After two weeks the animals in all groups were sacrificed and whole skin wound areas were removed and percentage of epithelization, scar formation, inflammation and angiogenesis were evaluated. Results indicated that at the end of the 2nd week the percentage of wound size were lowest in order of kefir 96h gel相似文献   

儿童囊性纤维化伴铜绿假单胞菌感染2例

对2018年4月至2019年6月四川省人民医院儿科收治的2例感染铜绿假单胞菌囊性纤维化(CF)患儿资料进行分析。例1为11岁女童,既往无反复呼吸道感染史,首次痰培养发现铜绿假单胞菌,反复纤维支气管镜检查下可见大量黄白色分泌物附着,胸部CT示双肺支气管周围多发斑点影及树芽征。CFTR基因检测结果示3处杂合突变:c.2909G>A(chr7:117246728)、c.*133T>A (chr7:117307295)和c.*125delT(chr7:117307285);例2为7岁男童,既往有反复呼吸道感染史,父母为近亲结婚,痰液及支气管肺泡灌洗液多次培养均为铜绿假单胞菌,且胸部CT示双肺支气管扩张并炎症。基因检测:染色体位置chr7-117171059,c.380T>G纯合突变发病,导致氨基酸改变p.leu127stop(无义突变)。提示对于儿童感染铜绿假单胞菌,胸部CT示支气管扩张征象及纤维支气管镜下见大量分泌物应警惕CF,需及时完善基因检测,尽早明确诊断。     

耐碳青霉烯类铜绿假单胞菌感染的危险因素及治疗的研究进展

铜绿假单胞菌(Pseudomonas aeruginosa,PA)是院内感染常见病原菌之一。碳青霉烯类抗生素是治疗铜绿假单胞菌重症感染的重要抗生素,近年来碳青霉烯类抗生素对铜绿假单胞菌耐药率呈明显上升趋势,给临床治疗带来巨大挑战。因此,分析耐碳青霉烯类铜绿假单胞菌(Carbapenem-resistance Pseudomonas aeruginosa,CRPA)感染的危险因素,了解其治疗的研究进展非常必要。本文从CRPA感染危险因素、治疗CRPA的新药、PA中多黏菌素耐药基因mcr-1基因、联合治疗方案和新的研究思路等方面对国内外最新研究进展进行综述。     

糖尿病健康教育对糖尿病患者治疗依从性和生活质量的影响

摘要：目的：研究糖尿病健康教育对糖尿病患者的护理效果。方法：选择2021年1月至2022年2月期间收治300例糖尿病患者,通过随机分组,对照组患者（n=150）实施常规干预,观察组患者（n=150）在对照组基础上,实施糖尿病健康教育,比较两组患者餐后2h血糖以及空腹血糖;护理前后的ESCA评分、认知程度评分;护理满意度、治疗依从性;护理前后的生活质量评分。结果：观察组患者的各项护理指标均优于对照组患者（P＜0.05）。结论：糖尿病患者通过糖尿病健康教育,护理效果显著,值得推广。     

2017—2020年克拉玛依市中心医院抗菌药物使用强度与分离菌耐药性变化情况

目的 分析克拉玛依市中心医院2017—2020年住院患者常见分离菌分布及耐药率的变迁情况,为指导抗菌药物临床合理应用提供依据。方法 收集2017—2020年克拉玛依市中心医院住院患者抗菌药物使用情况及分离菌,分析主要分离菌对常见抗菌药物的耐药率变化趋势。结果 2017—2019克拉玛依市中心医院抗菌药物使用率和使用强度呈下降趋势,4年共有分离菌12 135株,其中革兰阳性菌2 567株,占21.15%;革兰阴性菌6 310株,占52.00%;真菌3 258株,占26.85%。居前5位的分离菌分别是白色假丝酵母菌（19.59%）、大肠埃希菌（16.84%）、肺炎克雷伯菌（13.33%）、鲍曼不动杆菌（8.71%）和铜绿假单胞菌（7.94%）。主要分离菌对常用抗菌药物耐药率总体呈下降趋势,肠杆菌科细菌对临床常用抗菌药物耐药性下降最明显。结论 克拉玛依市中心医院分离菌逐年增加,耐药率有下降趋势,抗菌药物临床应用基本合理,但需完善真菌检测能力。     

下呼吸道铜绿假单胞菌的耐药性分析与相关危险因素探讨

目的对下呼吸道的铜绿假单胞菌的耐药性和相关危险因素进行探讨分析。方法在我院治疗的下呼吸道感染160例患者痰液标本分离出的菌株进行分析,分析多重耐药菌分布、耐药性和相关危险因素。结果我院下呼吸道感染以革兰阴性菌为主,绝大多数的菌株均有不同程度的耐药。抗生素滥用、有创检查等为铜绿假单胞菌耐药的危险因素。结论严格监控致病菌和高危因素,防止致病菌在医院内流行。     

Expression of Connective Tissue Growth Factor and Bone Morphogenetic Protein-7 in Pseudomonas aeruginosa-induced Chronic Obstructive Pulmonary Disease in Rats

Abstract

Repeated intratracheal injection of Pseudomonas aeruginosa (PA) in male Wistar rats was used to investigate the role of chronic infection in the development of chronic obstructive pulmonary disease (COPD) and the possible involvement of connective tissue growth factor (CTGF) and bone morphogenetic protein-7 (BMP-7) in this process. Injections of PA or normal saline solution were given for 8 weeks and the rats observed for a further 8 weeks. In addition to arterial blood gas, lung function and lung pathology measurement during this time period, protein and mRNA expression of CTGF and BMP-7 were measured, and the correlation of expression of CTGF and BMP-7 with pathological changes in the lung was evaluated. Repeated intratracheal PA infection in rats caused reduction in body weight, hypoxia, carbon dioxide retention, compromised lung function, chronic inflammation, thickening of the tracheal and arterial walls, and emphysema, changes consistent with those of COPD. Rats with PA infection also had increased CTGF and decreased BMP-7 expression, suggesting that both CTGF and BMP-7 are involved in the occurrence and development of airway remodeling. Our findings suggest that repeated airway infection is not only a factor resulting in deterioration of COPD, but is also a risk factor for its development, and that CTGF and BMP-7 are involved in the pathogenesis of this condition.     

糖皮质激素在老年患者蝮蛇咬伤致全身炎症反应综合征及多器官功能损伤中的应用价值

目的探讨小剂量氢化可的松琥珀酸钠在老年患者蝮蛇咬伤全身炎症反应综合征（SIRS）及多器官功能损伤中的应用价值。方法采用前瞻性研究方法,选择2009年4月至2013年4月我科收治的合并SIRS和多器官功能受损的蝮蛇咬伤老年患者60例,随机分为对照组和治疗组（n=30）,两组均给予常规程序化治疗,治疗组加用小剂量氢化可的松琥珀酸钠,测定两组患者治疗前及治疗后1,3,5d的炎症相关指标及血生化指标。结果治疗前两组各项指标比较,差异均无统计学意义（P〉0．05）。与治疗前比较,对照组肌酸激酶（CK）和肌酸激酶同工酶（CK—MB）治疗后第3天差异有统计学意义（P〈0．05）,体温（T）、脉搏（P）、白细胞计数（WBC）、C反应蛋白（CRP）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）第5天出现统计学差异（P〈0．05）;治疗组T,CK和CK-MB在第1天时差异即有统计学意义（P〈0．05）,WBC,CRP,AST,ALT和LDH在第3天时差异有统计学意义（P〈0．05）。治疗组与对照组间各项指标比较,3d时CK和CK-MB差异有统计学意义（P〈0．05）,5d时AST,ALT和LDH差异有统计学意义（P〈0．05）。结论小剂量氢化可的松琥珀酸钠能减轻老年患者蝮蛇咬伤的炎症反应,对重要器官的功能有一定的保护作用。     

羟基红花黄色素A对脑缺血-再灌注大鼠星形胶质细胞活性的影响

目的观察羟基红花黄色素A（HSYA）对脑缺血-再灌注大鼠星形胶质细胞活性的影响。方法将健康成年雄性SD大鼠12只随机分为HSYA组和对照组,每组6只。应用线栓法制备大鼠大脑中动脉缺血1．5h再灌注72h模型。成功阻断大脑中动脉血流后,HSYA组于缺血后30min,再灌注即刻,再灌注后24及48h分别经尾静脉注射HSYA,每次2mg／kg。对照组给予同体积的等渗盐水,其余与HSYA组相同。采用免疫组化染色和Western blot检测方法,观察HSYA对脑缺血后缺血半暗带区和核心区星形胶质细胞活化的标志物——胶质纤维酸性蛋白（GFAP）的影响。结果免疫组化染色显示,与对照组比较,HSYA组半暗带区GFAP阳性细胞数目增加,细胞突起增多、粗大,荧光染色强度增加（44±11比107±9）,P〈0．05;WesternBlot检测显示,GFAP蛋白水平亦高于对照组[（100％比（127±7）％,P〈0．05]。但免疫组化染色和WesternBlot检测结果显示,HSYA对缺血核心区GFAP阳性细胞荧光染色强度（36．5±5．4比42．0±1．9）、GFAP蛋白水平[100％比（105±13）％]均没有显著影响,均P〉0．05。结论活血化瘀中药HSYA可以增加脑缺血-再灌注大鼠缺血半暗带区星形胶质细胞的活性。