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991.
头孢硫脒与6种抗菌药物联用对金葡球菌的体外抗菌活性 总被引:1,自引:0,他引:1
目的:评价头孢硫脒分别与万古霉素、奈替米星、阿米卡星、环丙沙星、左氧氟沙星和加替沙星6种抗菌药物联合用药,对于临床分离的金葡球菌(SA)的体外联合抗菌效应.方法:经VITEK鉴定临床分离致病菌,采用棋盘法设计,微量肉汤稀释法测定6组抗菌药物对SA及耐甲氧西林金葡球菌(MRSA)的最低抑菌浓度,并计算部分抑菌指数(FIC指数). 结果:头孢硫脒与万古霉素、奈替米星、阿米卡星、环丙沙星、左氧氟沙星、加替沙星联合应用后,MIC50,MIC90明显降低,FIC指数<0.5占60%~90%,0.5~1占10%~30%,1~2占0~13.3%,>2为0.结论:头孢硫脒与6种抗菌药物联合应用后,对SA基本表现为协同和相加作用,说明联合用药的疗效要高于两药单独使用. 相似文献
992.
993.
994.
口蹄疫DNA疫苗海藻酸钠微球的制备及体外释放的研究 总被引:4,自引:0,他引:4
目的:研制DNA疫苗海藻酸钠微球,并对其体外释药特性进行考察。方法:以口蹄疫DNA疫苗为DNA疫苗的模型药物,采用喷雾干燥一离子交联法制备DNA疫苗海藻酸钠微球;考察粒径大小、外观、载药量等理化特性;考察微球的体外释药特性及其影响因素。结果:微球球形圆整,分散性好,平均粒径为11.9μm,载药量为5%,产率为53.2%。微球的体外释放速率受载药量影响较小,而壳聚糖的交联固化度增高,微球的体外释放速率变慢。结论:以生物降解材料海藻酸钠、壳聚糖,用喷雾干燥法制备DNA疫苗微球,不需要超声和有机溶剂,因而有利于DNA疫苗结构和功能的稳定性;工艺简便,易于工业化生产。 相似文献
995.
996.
Keiichi Ozono Sotaro Mushiake Toshikazu Takeshima Masahiro Nakayama 《Fetal and pediatric pathology》1997,17(2):249-258
We examined the placentas of 12 patients in whom congenital cytomegalovirus CMV infection was suspected from serological and or pathological evaluation. Seven patients died including four intrauterine deaths and five survived. On histological examination, the characteristic inclusion bodies were detected in only three placentas, and villitis with plasma cell infiltration was seen in eight placentas. Immunohistochemistry using a specific antibody against CMV improved the sensitivity of CMV detection 10 cases were positive . With the polymerase chain reaction PCR following the extraction of DNA from formaldehyde-fixed placenta samples, CMV DNA was detected in seven cases. All 12 subjects were diagnosed with CMV infection by additional Southern blot analysis after the PCR. CMV DNA was also detected by an in situ hybridization method in all cases. With current molecular biological techniques the placenta can be reliably used for the diagnosis of congenital CMV infection. 相似文献
997.
Infections by hepatotropic viruses belong to the most common complications of chemotherapy in children suffering from neoplastic
diseases. The rate of hepatitis B virus (HBV) or hepatitis C virus (HCV) infection and the effectiveness of passive immunization
against HBV were studied in 285 children; 148/285 with lymphoproliferative diseases and 137/285 with solid tumours. HBV infection
was observed in 10.2% children receiving hepatitis B immune globulin as compared to 36.8% without passive immunization against
HBV. Anti-HCV antibodies were similar in both groups amounting 38.7% and 32.6% respectively.
Conclusion The results show that hepatitis B immune globulin administration is effective and that HCV might become the main cause of
hepatitis among immunosuppressed patients in the future.
Received: 13 December 1994 / Accepted: 18 October 1996 相似文献
998.
E. Fombonne P. Bolton J. Prior H. Jordan M. Rutter 《Journal of child psychology and psychiatry, and allied disciplines》1997,38(6):667-683
First-degree relatives of 99 autism probands and of 36 Down's syndrome controls were assessed with standardised tests of intellectual functioning, reading, and spelling. Higher mean verbal IQ scores, and discrepancies in favour of verbal scores, were characteristic of autism relatives. No consistent differences were found on performance scales, reading, and spelling tests. Among autism relatives, siblings affected with the broad phenotype of autism had significantly lower IQ scores and poorer reading and spelling performances than unaffected siblings. However, the small size of the cognitive difference and the lack of a distinctive cognitive profile indicates that standardised cognitive measures used in this study are unlikely to improve the operationalised definition of the broad phenotype of autism. The slightly superior verbal performance of relatives in the autism group might represent some form of heterozygote advantage. 相似文献
999.
Edward C. Ditkoff Jennifer Plumb Amy Selick Mark V. Sauer 《Journal of assisted reproduction and genetics》1997,14(3):145-147
Purpose: Our purpose was to characterize and describe anesthesia practice in programs performing IVF in the United States.
Methods: We used a telephone survey requiring respondents to be either the program director, a physician, or a nurse familiar with
the practice. Two hundred seven (78%) Society of Assisted Reproductive Technology (SART) registered programs agreed to participate.
Programs were divided by geographic region and type of practice (academic versus private).
Results: Ninety-one private (68%) and 41 academic (56%) programs used personnel provided by the Department of Anesthesiology. Conscious
sedation was performed most commonly (95%). The remaining 5% used primarily either general, regional, or local anesthesia.
Typical recovery times were 90 to 120 min. Average costs of anesthetic administration were $300–$400 and were similar among
groups except for the Eastern academic programs, with a higher mean cost of $543. Programs using personnel from anesthesiology
reported higher costs compared to programs utilizing their own staff ($391±15 vs $157±11; P<0.05). Complications were infrequent
(<10%); no hospitalizations or serious life-threatening incidents were reported.
Conclusions: A large number of programs safely used their own trained personnel to deliver anesthesia, and realized a significant reduction
in cost. 相似文献
1000.
Hirokatsu Kida Michiyoshi Taga Hiroshi Minaguchi Makoto Hanazono Tomoo Ohashi Teruyo Sakakura Moriaki Kusakabe 《Journal of assisted reproduction and genetics》1997,14(1):44-50
Purpose: Our aim was to examine the changes in spatiotemporal tenascin (TN) expression in mouse uterus during early pregnancy, when
the uterine tissue undergoes a tremendous restructuring.
Methods: Using immunohistochemistry and in situ hybridization, the changes in distribution of TN protein in mouse uterine tissues
in pregnancy Day 0 through Day 5 were analyzed.
Results: Immunoreactive TN and TN mRNA were expressed in the basement membrane of the epithelium as well as in the smooth muscle layer,
and their distribution shifted from the subbasement region on Day 0–3 to the smooth muscle layer on Days 4 and 5.
Conclusions: These results indicate that TN expression in the uterus during early pregnancy is spatiotemporally different and may be regulated
by a different mechanism. 相似文献