以肾脏受累为主要表现的恶性高血压临床病理分析

目的了解以肾脏受累为主要表现的恶性高血压(MHPT)患者的临床与肾脏病理特征。方法回顾性分析我院肾内科11年来收治的27例(男21例,女6例,年龄19～51岁)MHPT患者的血压、眼底、尿和肾功能变化,对比分析原发性和肾实质性MHPT的临床与肾脏病理改变。结果27例MHPT患者中原发性、肾实质性和肾血管性分别为10例(37．0％)、10例(37．0％)和1例(3．7％),病因不明6例(22．3％)。急进性肾炎综合征和进行性肾功能损害是肾脏受累的主要表现。与肾实质性MHPT相比,原发性的MHPT尿蛋白量较少(P=0．001),多数病人有高血压家族史(7／10)。13例MHPT肾脏病理：原发性MHPT肾小动脉纤维素样坏死、内膜重度增生,呈现典型的洋葱皮样改变,肾小球为缺血性改变;肾实质性肾小动脉壁厚,细动脉玻璃样变,肾小球呈严重炎症病变。部分原发性MHPT经长期治疗肾功能可明显改善。结论以肾脏受累为主要表现的MHPT不少见,临床易误、漏诊;原发性与肾实质性MHPT的临床、肾脏病理改变及其预后均不同。     

Efficiency analysis of VersaPlex™ 27PY system in Central Indian Population: First report from Indian population

In the current scenario, DNA typing is the need of forensic science field due to its ability to provide results in much shorter time. In view of advancement of forensic DNA typing and incensement in the number of STRs markers, Promega offered a new VersaPlex™ 27PY system with 27 loci (23 autosomal STR loci, Amelogenin, DYS391 and two rapidly mutating Y-STR loci (DYS570 and DYS576)). In this study, the efficacy of “23 autosomal STR loci” for paternity testing and personal identification was demonstrated in Indian population. For this, 217 central Indians were tested and all the statistical parameters of forensic and population genetic interest were calculated. In addition, sensitivity of the kit was also tested for forensic casework. During investigation with VersaPlex™ 27PY system, allele 11 at locus TPOX was observed to be most frequent with the highest allelic frequency 0.432. Studied 23 loci showed valuable together with highest value of combined power of discrimination (CPD = 1), combined power of exclusion (CPI = 0.9999999989) and lowest value of combined matching probability (CPM = 7.92x10^-28).     

Pauci-immune rapidly progressive glomerulonephritis after nephrectomy in a renal donor

Idiopathic rapidly progressive glomerulonephritis (RPGN) is a clinicopathologic syndrome in which glomerular damage is accompanied by a rapid and progressive decline in renal function, usually resulting in irreversible renal failure in weeks or months. We report the occurrence of pauci-immune RPGN, more specifically microscopic polyarteritis nodosa (PAN), in a 60-year-old woman 15 months after donor nephrectomy, and 3 months after documentation of intact, residual renal function. The transplanted kidney continues to function well in the recipient, 6 years posttransplantation, and 4.5 years beyond destruction of the donor's contralateral kidney by RPGN. The donor underwent cadaveric renal transplantation after 2 years on dialysis, and at the 3-year mark has intact renal function. These intriguing observations strongly argue that host environmental factors, rather than intrarenal factors, play a major causative role in the pathogenesis of RPGN.     

牛蒡子口腔崩解片的处方研究

目的确定牛蒡子口腔崩解片的处方。方法采用正交设计法选择牛蒡子口腔崩解片的处方。结果确定牛蒡子口腔崩解片处方中主要的成分配比为:交联聚乙烯吡咯烷酮8%,微晶纤维素25%,α-乳糖25%。结论确定了牛蒡子口腔崩解片的处方,但是此片剂口感一般,须进一步研究。     

Benign and low-grade superficial endothelial cell neoplasms in the molecular era

Vascular tumors are the most common mesenchymal neoplasms of the skin and subcutis, and they encompass a heterogeneous group with diverse clinical, histological, and molecular features, as well as biological behavior. Over the past two decades, molecular studies have enabled the identification of pathogenic recurrent genetic alterations that can be used as additional data points to support the correct classification of these lesions. The purpose of this review is to summarize the available data related to superficially located benign and low-grade vascular neoplasms and to highlight recent molecular advances with the role of surrogate immunohistochemistry to target pathogenic proteins as diagnostic biomarkers.     

A novel rapidly mineralized biphasic calcium phosphate with high acid-resistance stability for long-term treatment of dentin hypersensitivity

ObjectivesTreating dental hypersensitivity (DH) rapidly and maintaining long-term effectiveness remains challenging. We aimed to address this problem by fabricating a novel rapidly mineralized biphasic calcium phosphate (RMBCP), which could rapidly elicit mineralization to form hydroxyapatite (HA) and perform excellent acid-resistant stability, thus effectively blocking the exposed dental tubules and protecting them from acid attack.MethodsRMBCP was firstly synthesized by precisely adjusting the molar ratio of acetic acid and calcium hydroxide and characterized by X-ray diffraction (XRD), X-ray fluorescence microprobe (XRF), Fourier-transform infrared (FTIR) spectrometer, scanning electron microscope (SEM), and transmission electron microscope (TEM). Subsequently, using a commercialized desensitizing agent, 45S5 bioglass (BG), as the control group, the mineralization performance of RMBCP was investigated in simulated body fluid (SBF), Dulbecco's modified eagle medium (DMEM), and even slightly acidic artificial saliva (pH=6.6). Moreover, the biocompatibility of RMBCP was studied. Finally, the tubule occlusion effect and acid-resistant stability of RMBCP were evaluated in vitro and in vivo.ResultsThe rapid mineralization behavior of RMBCP could easily adhere to the dentin surface and block the dentinal tubules completely in vitro and in vivo within 7days. RMBCP performed high acid-resistant stability to maintain the long-term therapeutic effect of DH treatment.SignificanceDeveloping novel bioactive calcium phosphate materials with the ability to trigger mineralization for HA formation rapidly will be an effective strategy for the long-term treatment of dentin hypersensitivity.