真皮下血管网岛状皮瓣抗菌力的实验研究

为研究真皮下血管网薄皮瓣用于感染创面的可行性，在家猪臀部两侧分别形成以旋髂深血管主干分支为蒂的传统岛状皮瓣和远侧５０％修薄的真皮下血管网岛状皮瓣。用细菌计数、吖啶橙荧光染色测定白细胞吞噬指数和白细胞内杀菌率，应用激光多普勒、墨汁灌注、透明标本等方法，对两种皮瓣的抗菌力作自身对照研究。结果表明，真皮下血管网岛状皮瓣的抗菌力明显降低，可能与皮瓣修薄后血供减少，白细胞功能相应降低有关。     

~(125)碘-绒毛膜促性腺激素的电泳纯化及分析

标记抗原在贮存过程中,由于受幅射分解和脱碘影响而使免疫活性和生物活性降低。我们对自制的~(125)碘-绒毛膜促性腺激素进行了实验观察。结果显示:贮存1月后,其免疫活性和生物活性分别降低了33.1％和44.0％;经电泳纯化后的标记物的免疫活性和生物活性明显提高,分别达到了最初的90.5％和95.3％。     

天蚕素A-蛙皮肽杂合肽基因的表达及产物抗菌活性测定

目的 研究天蚕素A-蛙皮肽杂合肽基因[CA（1-8）-MA（1-12）]抗菌肽的抗菌活性。方法 将天蚕素A-蛙皮肽杂合肽基因抗菌肽的人工基因克隆到具有自切割功能的表达载体pTYB12上，并在大肠杆菌（E.coli)BL21(DE3)中进行表达，表达产物用几丁质树脂进行了亲和吸附，自切割后洗脱得到抗菌肽，进行透析以去除盐类物质，最后用大肠杆菌与金黄色葡萄球菌进行活性检测。结果 天蚕素A-蛙皮肽杂合肽[CA（1-8）-MA（1-12）]对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性。结论 天蚕素A-蛙皮肽杂合肽具有抗菌活性。其抑瘤活性的研究还有待于进一步研究。     

精子顶体酶活性检测对诊断男性不育症的价值

目的比较各种质量精液的精子顶体酶活性。方法分光光度比色法检测顶体酶活性。结果各种质量精液的精子顶体酶活性均显著低于对照组P<0.001。结论精子顶体酶可作为了解精子生育力的一项可靠指标。     

握力大小与前臂肌肉表面肌电活动模式的相关性研究

目的 研究手部握力大小与前臂肌肉表面肌电信号特征的相关性.方法 9名健康被试者参加了试验,包括最大自主收缩力(maximum volunteer contraction,MVC),80% MVC及40% MVC,记录指浅屈肌和腕长伸肌的表面肌电信号,并采用均方根和小波分析方法提取其特征参数;运用SPSS软件统计以被试者自身的MVC为基准的不同握力水平的相对特征值,使不同的被试者具有可比性.结果 对于单个被试者握力水平与表面肌电活动模式存在正相关性,即握力越大则对应的表面肌电信号的特征值也越大;对于所有的被试者这种正相关性也存在.结论 握力水平与肌肉活动模式具有相关性,这提示通过表面肌电信号的特征值不但可以预测握力的大小,还可用于运动功能测试及康复的评价.     

牡蛎功能短肽的制备及ACE抑制活性

以新鲜无壳牡蛎为原料,采用酶水解的方法制备牡蛎短肽,经SephadexG 15分离,并用HPLC测定其相对分子质量分布,通过HPLC法定量马尿酸测定各组分的ACE(血管紧张素转化酶)抑制活性。结果表明,牡蛎水解液中相对分子质量较大和较小部分的ACE抑制活性偏低,只有相对分子质量在一定范围内的短肽,对ACE具有较好的抑制作用,质量浓度为0.4mg/mL的牡蛎功能短肽的ACE抑制率为51.4%.     

How reproducible is cutaneous electrogastrography? An in-depth evidence-based study1

AIM: To check on reproducibility of parameters of the cutaneous electrogastrogram registered at a close or a distant time span. METHODS: Twenty-two volunteers recruited by an advertisement (11 females and 11 males, median age 25 years, range: 18-35) underwent three surface electrogastrography examinations of which two were taken on consecutive days and the third one was accomplished at least 2 weeks before or after the two other sessions. The examination involved a 30-min fasted recording, followed by a 90-min postprandial registration after intake of a 394-kcal mixed solid-liquid test meal. RESULTS: Parameters of the electrogastrogram pertaining to the frequency of the gastric slow waves exhibited good to moderate reproducibility, whereas fair reproducibility characterized parameters expected to describe the power of gastric slow waves. With the exception of the difference fed minus fasted power (DeltaDP), in no instance was the medium term reproducibility any worse than the short term one. Categorical data analysis revealed that the relative time share of normogastria postprandially exhibited a better reproducibility than in the fasted period. The Cohen's kappa-value of 0.459 for the DeltaDP for the medium term reproducibility placed this parameter within the range of moderate agreement between repeat examinations. Of the two two-parameter combinations considered, the alliance of the fasted and fed normogastria performed worse than any of those parameters considered alone, whereas a combination of the DeltaDP with the fed-state normogastria revealed a kappa-value amounting to 0.510 for the medium term reproducibility. CONCLUSIONS: The feasibility of some electrogastrographic parameters to convey clinically useful information may be hampered by their fair reproducibility. Recoding of parameters of the cutaneous electrogastrogram from primary continuous to secondary categorical may help achieve a better agreement between repeat examinations.     

无溶剂体系合成1,3-甘油二酯用脂肪酶的筛选及其酯化性质

对不同来源的脂肪酶进行了系统的筛选,并根据位置专一性的差别将脂肪酶分为1,3 特异的、1,3 选择性和非选择性的3类.采用LipozymeRMIM在无溶剂系统中催化甘油、脂肪酸合成1,3 sn 甘油二酯.研究表明脂肪酸、底物醇种类、温度、水分活度对LipozymeRMIM的活力有明显的影响.     

Effects of chelating agents on the rheological property of cervical mucus

As an ongoing effort to elucidate the mechanisms involved in the calcium-dependent fertility regulation process, the viscoelastic properties of the mucus obtained from lamb cervix and human semen, as well as their water and total protein contents after exposure to EDTA, a chelating agent, or Nonoxynol-9 (N-9), a spermicidal agent, were examined. The viscosity was measured using a Cone Plate Digital Viscometer, while the water and total protein contents were determined by the lyophilization process and the Lowry method, respectively. The significant changes in the rheological properties of mucus, such as its viscosity and the water content, upon exposure to EDTA were demonstrated. The viscosity of cervical mucus and human semen were significantly increased by EDTA treatment (as compared to the controls): lamb cervical mucus (2.9 ± 0.3 vs. 2.2 ± 0.3 cps) and human semen (5.0 ± 0.3 vs. 4.3 ± 0.3 cps), respectively. The hydration rate was decreased by EDTA treatment as compared with the control (93.6 ± 0.7 vs. 96.8 ± 0.8%). Among tested samples, the reduction in the percentage of sperm penetration through the cervical mucus was the highest in the mucus containing EDTA, which had the lowest water content (93.6 ± 0.7%), indicating that there is a positive relationship between the hydration rate of the cervical mucus and its ability to permit the penetration of spermatozoa. This result indicates that spermicidal activity exerted by high concentrations of EDTA is in part due to its effect on the rheological properties of cervical mucus or semen.     

刺山柑化学成分及其药理活性研究进展

刺山柑是一种山柑属植物,在多个国家药用。传统用作驱虫药、泥敖剂、利尿剂、通便剂、止痛剂和滋补剂。在我国新疆、西藏地区被广泛用于治疗痛风、类风湿性关节炎等。现代研究发现该植物含有挥发油、芥子油苷类、生物碱、萜类、黄酮类等多种成分,具有保肝、抗炎、抗病毒、抗氧化、免疫调节、降血糖、降血脂等多种生物活性。现对该植物化学成分和研究进展进行综述,以利于该植物的研究、开发与利用。