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991.
Popoli P Blum D Martire A Ledent C Ceruti S Abbracchio MP 《Progress in neurobiology》2007,81(5-6):331-348
The aim of this review is to summarize and critically discuss the complex role played by adenosine A2A receptors (A2ARs) in Huntington's disease (HD). Since A2ARs are mainly localized on the neurons, which degenerate early in HD, and given their ability to stimulate glutamate outflow and inflammatory gliosis, it was hypothesized that they could be involved in the pathogenesis of HD, and that A2AR antagonists could be neuroprotective. This was further sustained by the demonstration that A2ARs and underlying signaling systems undergo profound changes in cellular and animal models of HD. More recently, however, the equation A2A receptor blockade = neuroprotection has appeared too simplistic. First, it is now definitely clear that, besides mediating ‘bad’ responses (for example, stimulation of glutamate outflow and excessive glial activation), A2ARs also promote ‘good’ responses (such as trophic and antinflammatory effects). This implies that A2AR blockade results either in pro-toxic or neuroprotective effects according to the mechanisms involved in a given experimental model. Second, since HD is a chronically progressive disease, the multiple mechanisms involving A2ARs may play different relative roles along the degenerative process. Such different mechanisms can be influenced by A2AR activation or blockade in different ways, even leading to opposite outcomes depending on the time of agonist/antagonist administration. The number, and the complexity, of the possible scenarios is further increased by the influence of mutant Huntingtin on both the expression and functions of A2ARs, and by the strikingly different effects mediated by A2ARs expressed by different cell populations within the brain. 相似文献
992.
Ageing and Toll-like receptor expression by innate immune cells in chronic human schistosomiasis 下载免费PDF全文
Comin F Speziali E Martins-Filho OA Caldas IR Moura V Gazzinelli A Correa-Oliveira R Faria AM 《Clinical and experimental immunology》2007,149(2):274-284
There has been no systematic study of the immune response of individuals aged over 60 years living in Schistosomiasis mansoni-endemic areas, although senescence is reportedly associated with susceptibility to infection and progressive decline in immune function. We have shown previously, in two endemic areas in Minas Gerais, Brazil, that the frequency of individuals aged over 60 years with chronic schistosomiasis is no longer negligible. Moreover, several elderly individuals who have always lived in these endemic areas stay protected from infection. An important question for studies of ageing and disease control in developing countries is which differences in the immunological profile of these negatively tested (non-infected) individuals can account for their resistance to either infection or reinfection. We show, in the present study, that non-infected (negative) elderly individuals develop innate immune mechanisms of protection that replace the age-associated decline in T cell function. Non-infected elderly individuals from endemic areas of schistosome infection present an increase in the frequency of the natural killer (NK) CD56(low) subset of NK cells expressing Toll-like receptors (TLR)-1, -2, -3 and -4 as determined by flow cytometry analysis. In addition, the proportion of dendritic cells expressing TLR-1 is elevated as well as the frequency of monocytes expressing TLR-1 and -4. These results suggest that TLR expression by cells of the innate immune system may be related to the negative status of infection in some elderly individuals who are constantly exposed to S. mansoni. Developing mechanisms of protection from infection may represent a biomarker for healthy ageing in this population. 相似文献
993.
Estefanía E Gómez-Lozano N Portero F de Pablo R Solís R Sepúlveda S Vaquero M González MA Suárez E Roustán G Vilches C 《Tissue antigens》2007,70(1):34-41
Herpes simplex virus type 1 (HSV-1) causes lifelong latent infections in most humans. Periodical virus reactivations from latency in the neurons of sensitive ganglia lead to transport to mucocutaneous regions and productive replication, which results in recurrent inflammatory herpetic lesions or in asymptomatic virus shedding. The medical consequences of such lesions and the frequency of recurrences vary greatly in different subjects. Furthermore, many infected individuals never suffer manifestations of the disease, even when exposed to stimuli that trigger clinical recurrences in other humans. The origin of the variability in the clinical course of HSV-1 infection remains unexplained. Herpesviruses and other pathogens sabotage the expression of major histocompatibility complex class I molecules by infected cells, thus subverting T-cell-mediated immunity. Subversion of antigen presentation is counteracted by natural killer cells, which survey the human leukocyte antigen (HLA) expression by specific receptors. These include the killer cell immunoglobulin-like receptors (KIRs), which are encoded by a complex of extremely diverse and rapidly evolving genes. Here, we analyze the contribution of KIR gene diversity to the variable clinical course of HSV-1 infection by comparing the distribution of these genes in humans with clinical manifestations of the disease with that in asymptomatically infected donors. This study provides preliminary evidence that the receptors KIR2DL2 and KIR2DS2 predispose to symptomatic HSV-1 infection and favor the frequently recurring forms of the disease. Possible contribution of the 'HLA-C1' ligand to HSV-1 disease was not statistically supported. Because of an absolute genetic linkage between KIR2DL2 and KIR2DS2, we could not determine which receptor was primarily responsible for the observed association, but our results suggest that presence in the genome of KIR2DL2 and KIR2DS2 hinders an effective cellular response to HSV-1. 相似文献
994.
995.
Koval'chuk LV Khoreva MV Varivoda AS Pashchenko OE Gracheva LA Bykova LP Kondratenko IV Bologov AA 《Bulletin of experimental biology and medicine》2007,144(1):63-65
The production of TNF-α and IFN-α cytokines by peripheral blood mononuclears in response to stimulation by TLR2/6, TLR4, TLR5,
TLR9 ligands (zymosan, LPS, flagellin, and CpG-oligodeoxynucleotide, respectively) was studied in donors and patients with
common variable immunodeficiency. Individual characteristics of TNF-α production by mononuclears were revealed in donors.
Reduced stimulated production of TNF-α in response to stimulation with TLR4 and TLR5 ligands in vitro was detected in patients with common variable immunodeficiency.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 7, pp. 68–71, July, 2007 相似文献
996.
Lishmanov AY Maslov LN Lasukova TV Crawford D Wong TM 《Bulletin of experimental biology and medicine》2007,143(2):187-190
Intravenous pretreatment with κ-opioid receptor antagonist (−)-U-50,488 (1 mg/kg) improved heart resistance to the arrhythmogenic
effect of coronary occlusion and reperfusion. Selective κ1-opioid receptor antagonist norbinaltorphimine and nonselective blocker of peripheral opioid receptors methylnaloxone abolished
this antiarrhythmic effect. Preliminary blockade of protein kinase C with chelerythrine or inhibition of ATP-dependent K+ channels (KATP channels) with glybenclamide abolished the antiarrhythmic effect of κ-opioid receptor activation. Selective inhibitor of
sarcolemmal KATP channels did not modulate the κ-opioid receptor-mediated increase in cardiac electrical stability. Our results suggest that
protein kinase C and mitochondrial KATP channels play an important role in the antiarrhythmic effect associated with activation of peripheral κ-opioid receptors.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 2, pp. 145–148, February, 2007 相似文献
997.
Dushkin MI Khoshchenko OM Posokhova EN Schvarts YSh 《Bulletin of experimental biology and medicine》2007,144(5):713-716
We studied the effect of agonists of peroxisome proliferator-activated receptors α and γ and retinoid X receptors on the concentration
and synthesis of lipids in macrophages of C57Bl/6 mice with inflammation induced by intraperitoneal injection of zymosan.
We revealed a significant increase in [1-14C]oleate incorporation into cholesterol esters and triglycerides, increase in the content of free cholesterol, cholesterol
esters, and triglycerides, and formation of oil red-stained lipid inclusions in peritoneal macrophages 24 h after administration
of zymosan in a dose of 50 mg/kg. Treatment with agonists of retinoid X receptors and peroxisome proliferator-activated receptors
α and γ 30 min before and 12 h after zymosan injection decreased the synthesis of triglycerides and cholesterol esters, reduced
the content of free cholesterol, cholesterol esters, and triglycerides in macrophages, and prevented the formation of cytoplasmic
lipid inclusions in macrophage-derived foam cells during inflammation.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 11, pp. 556–559, November, 2007 相似文献
998.
Boyko SS Kolyvanov GB Zherdev VP Gudasheva TA Kiryanova EP Seredenin SB 《Bulletin of experimental biology and medicine》2007,144(3):312-313
Pharmacokinetics of an original compound GB-115 (N-phenylhexanoylglycyltryptophan) synthesized on the basis of the structure
of endogenous tetrapeptide cholecystokinin-4 was studied by means of high-performance liquid chromatography. Pharmacokinetic
parameters were calculated after intravenous and peroral administration of GB-115. Our results indicate that this dipeptide
is resistant to peptidases. The absolute bioavailability of GB-115 is 4.65%.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 9, pp. 285–287, September, 2007 相似文献
999.
van de Vrede Y Fossier P Baux G Joels M Chameau P 《Pflügers Archiv : European journal of physiology》2007,455(2):297-308
In several neuronal preparations, the ryanodine-sensitive calcium store was reported to participate in the generation of slow
afterhyperpolarization currents (IsAHP) involved in spike frequency adaptation. We show that calcium release from the ryanodine-sensitive
calcium store is a major determinant of the triggering of IsAHP in mouse CA1 pyramidal neurons. Whole-cell patch clamp recordings
in hippocampus slices show that the intracellular calcium stores depletion using an inhibitor of the endoplasmic reticulum
Ca2+-ATPase (5 μM cyclopiazonic acid), as well as the specific blockade of ryanodine receptors (100 μM ryanodine) both reduced
the IsAHP by about 70%. Immunohistology, using an anti-RyR3 specific antibody, indicates that RyR3 expression is particularly
enriched in the CA1 apical dendrites (considered as the most important site for sAHP generation). We show that our anti-RyR3
antibody acts as a functional RyR3 antagonist and induced a reduction in IsAHP by about 70%. The additional ryanodine application
(100 μ M) did not further affect IsAHP, thus excluding RyR2 in IsAHP activation. Our results argue in favor of a specialized
function of RyR3 in CA1 pyramidal cells in triggering IsAHP due to their localization in the apical dendrite. 相似文献
1000.
Diniz GP Carneiro-Ramos MS Barreto-Chaves ML 《Pflügers Archiv : European journal of physiology》2007,454(1):75-81
Increased thyroid hormone (TH) levels are known to induce cardiac hypertrophy. Some studies have provided evidence for a functional
link between angiotensin II (ANG II) and transforming growth factor β1 (TGF-β1) in the heart, both being able to also induce
cardiac hypertrophy. However, the contribution of this growth factor activated directly by TH or indirectly by ANG II in cardiac
hypertrophy development remains unknown. To analyze the possible role of TGF-β1 in cardiac hypertrophy induced by TH and also
to evaluate if the TGF-β1 effect is mediated by ANG II receptors, we employed Wistar rats separated into control, hypothyroid
(hypo) and hyperthyroid (T4 − 10) groups combined or not with ANG II receptor blockers (losartan or PD123319). Serum levels
of T3 and T4, systolic pressure and heart rate confirmed the thyroid state of the groups. The T4 − 10 group presented a significant
increase in cardiac TGF-β1 levels; however, TGF-β1 levels in the hypo group did not change in relation to the control. Inhibition
of the increase in cardiac TGF-β1 levels was observed in the groups treated with T4 in association with losartan or PD123319
when compared to the T4 − 10 group. These results demonstrate for the first time the TH-modulated induction of cardiac TGF-β1
in cardiac hypertrophy, and that this effect is mediated by ANG II receptors. 相似文献