~3H-TdR掺入法测定NHG-1脾淋巴细胞转化功能

本文报告用~3H-TdR掺入法测定本室的NC系正常裸小鼠和荷人脑胶质瘤裸小鼠(NHG-1)的脾淋巴细胞转化功能。结果提示:正常的纯合子(nu/nu)T细胞缺乏,B细胞功能均正常;杂合子(nu/ )的T和B细胞功能均正常;荷瘤鼠(NHG-1,30代)除了T细胞缺乏,B细胞功能亦受到抑制。     

不稳定型桡骨远端骨折的外固定器治疗

目的：探讨外固定器治疗不稳定型桡骨远端骨折的疗效。方法：对12例不稳定型桡骨远端骨折的患者采用改良AO外固定器治疗，术后随访5－8个月，根据骨折愈合情况和腕关节形态及功能情况，按Jakim评分结果评价治疗效果。结果：骨折愈合时间为6－8周，优良率为91％。结论：外固定器治疗不稳定型桡骨远端骨折具有良好的固定作用，能较好地防止骨折畸形愈合和保护腕关节功能。     

Prolonged but not acute fluoxetine administration produces its inhibitory effect on hippocampal seizures in rats

This study assessed the effects of acute as well as long-term administration of fluoxetine, a selective serotonin (5-HT) reuptake inhibitor with anti-depressant properties, on hippocampal (HIP) seizures elicited by electrical stimulation in rats. The fluoxetine effect on HIP seizures was also assessed following long-term treatment with gepirone, a 5-HT_1A receptor agonist. Acute single administration of fluoxetine (1, 10 mg/kg; IP) was found to produce no significant effect on HIP seizure activity. Following daily IP administration of fluoxetine (10 mg/kg per day) or gepirone (10 mg/kg per day) for 21 days, animals were given a 7-day drug-free period and then challenged with an acute dose of 10 mg/kg fluoxetine. These treatment regimens resulted in a significantly increased afterdischarge threshold of HIP seizures in response to acute fluoxetine administration. The inhibitory effect of fluoxetine, however, was not present 4 weeks after long-term treatment with either fluoxetine or gepirone. The present results indicate that long-term treatment with these compounds enhances the antiepileptic effect of subsequent fluoxetine administration on the generation of HIP seizures. This effect is possibly related to the well-demonstrated evidence that fluoxetine and gepirone, on long-term treatment, facilitate net 5-HT neurotransmission through desensitization of presynaptic 5-HT autoreceptors.     

儿童及少年期首发精神障碍患者的随访

目的了解儿童及少年期情感障碍和精神分裂症的临床特征.方法收集1984年至1999年之间在本院就诊患者中符合DSM-Ⅲ情感障碍和精神分裂症诊断的儿童及少年期首次发作病例64例,年龄5～17岁.入组时进行基线评定,然后长期随访.至随访结束,对两组疾病进行对照分析.结果58例（91%）完成随访,平均随访时间12.4年.儿童少年期入组诊断为情感障碍者36例,至成年随访结束时,92%诊断为情感障碍,8%诊断为精神分裂症;儿童少年期入组诊断为精神分裂症者22例,至成年随访结束时,32%诊断为情感障碍,68%诊断为精神分裂症.儿童少年期情感障碍患者在成年期诊断为精神分裂症者明显高于精神分裂症患者修改诊断为情感障碍（χ^2=22.852,P＜0.001）.儿童少年期精神障碍至成年期的诊断一致性较低（Kappa=0.62）.儿童及少年期发作的情感障碍与精神分裂症比较,起病年龄低、发作次数少、社会关系良好和较好的社会功能.情感障碍的精神症状多与精神分裂症重叠,但精神分裂症出现频率较高的情感不协调、注意力分散、关系妄想、情感迟钝、被洞悉感等症状,在情感障碍患者中始终未存在.结论儿童及少年期的情感障碍在症状学上与精神分裂症有许多相似之处,是导致误诊的重要原因.但儿童少年期精神分裂症存在的一些核心症状可能是两者鉴别诊断的症状学指标.     

医院电子天平维护计量情况统计分析

电子天平具有体积小，功能齐全，操作简单，数值直观等优点，正为愈来愈多的医院、学校、科研单位所使用。本文结合我院五年中电子天平的使用、维护、计量情况，统计分析了其常见故障及电子天平使用中的注意事项。对保证医院电子天平使用过程的量值准确，提高临床诊断及科研水平有所帮助。     

The Durability of Laparoscopic Nissen Fundoplication: 11-Year Outcomes

Laparoscopic Nissen fundoplication (LNF) has become the most commonly performed antireflux procedure since its introduction in 1991. There are few studies with greater than 5-year outcomes. Herein we report a series of 312 consecutive patients who underwent primary LNF before 1996. Follow-up of more than 6 years was available in 166 patients, and the mean follow-up was 11 years (median 11.1 years, range 6.1–13.3 years). Prospective data collection included preoperative and current symptom scores (scale 0 = none to 3 = severe), as well as the level of patient satisfaction and use of antireflux medications. Total symptom score for each patient was summed from seven symptoms for a maximum value of 21. Heartburn and regurgitation were the most improved symptoms; however, all symptoms were significantly improved (P < 0.01). The total symptom score at follow-up was 2.6 down from 7.5 at baseline, with a mean difference of −4.9 (range −12 to 3). The percentage of patients stating they would have the procedure again was 93.3%, and 70% were off daily antireflux medications. Outcomes at a mean of 11 years after LNF are excellent, and the majority of patients had their symptoms resolved or significantly improved and are satisfied with their results. Presented at the 47th Annual Meeting of the Society for Surgery of the Alimentary Tract, May 22, 2006, Los Angeles, CA     

神经病理性疼痛老年大鼠海马CA1区突触长时程增强的变化

目的观察神经病理性疼痛老年大鼠海马CA1区突触长时程增强(LTP)的变化。方法老年雄性Wistar大鼠15只,随机均分为三组。模型组,结扎左侧L4/L5脊神经;D-2-氨基-5-磷酸戊酸(AP5)组,侧脑室输注AP5,余同模型组;假手术组,操作同模型组,但不结扎。观察大鼠行为变化,连续3周测定大鼠电痛阈值,1次/周;记录海马CA1区树突层兴奋性突触后膜电位(EPSP)、输入/输出曲线及LTP的变化。结果模型组与AP5组大鼠术后行为异常,术后各时点电痛阈值低于术前值及假手术组相应时点值(P<0.05);三组基础EPSP幅值稳定,最大基础EPSP幅值的50%组间比较差异无统计学意义;模型组LTP高于其他两组(P<0.05)。结论结扎老年大鼠L4/L5脊神经所致的神经病理性疼痛,不干扰海马CA1区突触及锥体细胞兴奋性,但可易化该区突触LTP。     

关于河南中医学院设置健康保险专业的思考

目的：探讨河南中医学院设置健康保险专业的论证。方法：从我国健康保险业的发展实际出发，集中分析河南中医学院设置健康保险专业的必要性，论证了河南中医学院在师资、设施、生源与就业等方面的可行性，以培养目标、课程设置和实习基地建设为轴线，提出了设置健康保险专业的建议。结果与结论：河南中医学院设置健康保险专业有必要、有可行性、有优势。     

63例扩张型心肌病的长期随访分析

目的:评价扩张型心肌病的远期预后。方法:扩张型心肌病患者63例,给予强心、利尿、β受体阻滞剂、血管紧张素转化酶抑制剂及中药生脉(参麦)复方制剂治疗,定期随访,随访时间3～13年。随访项目包括:心功能改善情况、体力活动适应程度、院外服药治疗、再次入院次数,死亡,了解并登记死亡原因。结果:63例中随访3～5年16例,5～10年35例,10年以上12例,平均随访时间6.1年。随访期间死亡18例(病死率28.6%);存活45例,存活者患病时间2～15年,其中28例能从事一般正常劳动及工作(62.2%),生活可自理者10例(22.2%)。结论:由于治疗方案的改进,特别是中西医结合药物的应用,使扩张型心肌病患者的预后改善,平均存活时间延长,多数患者的生存质量提高。