血栓闭塞性脉管炎患者血栓素、前列环素及血液流变学指标的检测分析

目的 :探讨血栓闭塞性脉管炎患者血栓素B2 、前列环素与血液流变学指标的变化及其与发病机制的关系。方法 :用放射免疫法测定TXA2 和PGI2 的代谢产物血栓素B2 (TXB2 )、6 酮 前列腺素F1α( 6 K PGF1α) ,LBY N6A自清洗旋转式粘度计测定血液流变学指标 ,并与对照组对比分析。结果 :血栓闭塞性脉管炎患者TXB2 ( 53.59± 83.2 1ng/L)、6 K PGF1α( 14 .50± 3.4 5ng/L) ,与对照组相比差异显著 ;全血高切粘度、低切粘度、血浆粘度及纤维蛋白原疾病组均高于对照组 ,差异具有统计学意义。结论 :血栓闭塞性脉管炎患者存在TXA2 /PGI2失衡 ,并与血液流变学的改变有密切关系     

口服Ⅱ号避孕药对血小板功能及血液凝固的影响

为了解口服Ⅱ号避孕药对血小板功能及血液凝固性的影响,本文对65名连续服药妇女进行了研究。她们的年龄为27～45(平均36.5)岁。服药期限1～17(平均6.7)年。测定项目有纤维蛋白原,血小板粘附率,血小板聚集率,Ⅷ因子相关抗原,抗凝血酶活力等。20例未服药妇女作对照。测定结果服药组血小板粘附率、聚集率及纤维蛋白原比对照组明显增高(P<0.001)。Ⅷ因子相关抗原略增高,但无统计学意义。抗凝血酶活力显著降低(P<0.001)。本文就急性心肌梗塞及血栓栓塞的潜在可能性等问题进行了讨论,并对预防此类并发症提出了建议。     

HLö 7 dimethanesulfonate,a potent bispyridinium-dioxime against anticholinesterases

HLö 7 dimethanesulfonate (1-[[[4-(aminocarbonyl)pyridinio] methoxy] methyl] -2,4-bis [(hydroxyimino) methyl]pyridinium dimethanesulfonate) is a broad-spectrum reactivator against highly toxic organophosphorus compounds. The compound was synthesized by a new route with the carcinogenic bis(chloromethyl)ether being substituted by the non-mutagenic bis(methylsulfonoxymethyl)ether. The very soluble dimethanesulfonate of obidoxime was also prepared by this way. HLö 7 dimethanesulfonate is the first water-soluble salt of HLö 7 that should be suitable for the wet/dry autoinjector technology, because aqueous solutions of HLö 7 are not very stable (calculated shelf-life 0.2 years when stored at 8°C, 1 M solution, pH 2.5). The crystalline preparation contains 96% of thesyn/syn-isomer, less than 2% of thesyn/anti-isomer and some minor identified by-products. HLö 7 was very efficient in reactivating acetylcholinesterase (AChE) blocked by organophosphates as long as ageing did not prevent dephosphylation. HLö 7 was superior to HI 6 (1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]pyridinium dichloride) in reactivating soman and sarin-inhibited AChE from erythrocytes, and literature data indicate that HLö 7 exceeds HI 6 by far in reactivating tabun-inhibited AChE. In atropine-protected, soman-poisoned mice HLö 7 was three times more potent than HI 6 (protective ratio 5 versus 2.5), and in sarin-poisoned mice HLö 7 was 10 times more potent than HI 6 (protective ratio 8 for both oximes). In atropine-protected guinea-pigs HLö 7 was less effective than HI 6 (protective ratio: 2.3 versus 5.2 for soman; 5.2 versus 6.8 for sarin; 4.3 versus 3.8 for tabun). The mean survival time of anaesthetized guinea-pigs exposed to 5 LD₅₀ soman (6.3 min) was increased by atropine (27 min) and atropine + HLö 7 (57 min). HLö 7 alone did not prolong the survival. The most impressive effect of HLö 7 was on respiration: 3 min after i.v. injection of HLö 7 and atropine, the depressed respiration increased rapidly to 60% of control and remained at that level during the observation period (60 min). With atropine alone, respiration recovered only slowly. Behavioural and physiologic parameters were determined in atropine-protected mice exposed to a sublethal soman dose. The running performance was significantly improved by HLö 7. Even central symptoms, e.g. hypothermia and convulsions, were decreased markedly by HLö 7 (evaluation 60 min after poisoning). The pharmacokinetic data for HLö 7 in male beagle dogs are similar to those of HI 6. After i.v. injection: t_1/2 = 5 min; t_1/2ß = 46 min; V_D = 0.24 1/kg; Cl_p1 = 3.7 ml x min^–1 x kg^–1; Cl_ren= 3.2 ml x min^–1 x kg^–1; renal excretion of unchanged HLö 7 = 86%. After i. m. injection: t_1/2abs = 14 min; t_1/2ß = 48 min; Vd = 0.27 1/kg; Cl_p1= 3.9 ml x min^–1 x kg^–1; Cl_ren= 2.7 ml x min^–1 x kg^–1; renal excretion of unchanged HLö 7 = 76%; bioavailability >95%. Plasma protein binding was <5%; HLö 7 did not permeate into red cells. A dose of 20 mol/kg was well tolerated both after i.v. and i.m. administration. In anaesthetized dogs (chloralose) HLö 7 i.v. (20 (imol/kg) showed marginal hypotensive effects, whereas 50 mol/kg resulted in decreased mean blood pressure (–15%) and blood flow (–30%) without reflex tachycardia. One out of four dogs developed a circulatory shock syndrome with anuria. Respiration varied only transiently. Blood gases and pH were not influenced. Similar cardiovascular effects were observed in anaesthetized (urethane) guinea-pigs. In isolated guinea-pig hearts (Langendorff) sinus and ventricular heart rate were not influenced by HLö 7 <500 M. HLö 7 antagonized both carbachol and nicotine effects. Red cell AChE was inhibited by HLö 7 by up to 50%; C₅₀ about 100 M. Previously, HLö 7 was shown to block ganglionic transmission (IC₅₀= 500 M), probably due to ion-channel blockade. These data indicate that HLö 7 combines ganglion blocking, anticholinergic and indirect cholinergic properties like other bispyridinium compounds. The results suggest that HLö 7 may be tolerated by man at a dose of 10 mol/kg. Vital functions are not expected to be impaired. At such a dose (250–500 mg), which can be injected by an autoinjector, HLö 7 is expected to be superior to HI 6.Part of thesis     

In situ demonstration of T cell subsets in atrophic parapsoriasis

It has been demonstrated recently in mycosis fungoides and lichen planus that T lymphocyte subsets may be identified in cutaneous lymphocytic infiltrates using the immunoperoxidase technic in conjunction with monoclonal antibodies produced by the technic of Kohler and Milstein. This communication describes the application of this technic to cutaneous lymphoid infiltrates of parapsoriasis in which T cell predominance has been demonstrated previously. The lymphoid infiltrates of six patients with atrophic parapsoriasis were examined by the indirect immunoperoxidase technic using monoclonal antibodies (from two commercial sources) directed against "helper" and "suppressor" T cell subsets. Both "helper" and "suppressor" cells (as defined by a positive reaction with monoclonal antibodies) could be identified in cutaneous infiltrates. "Helper" cells predominated, but in varying degrees among patients. The relevance of these findings in relation to the possible development of clinical mycosis fungoides from atrophic parapsoriasis is discussed. In addition, factors causing difficulty in the consistent identification of cell subtypes are discussed. These factors suggest that in the present state of imperfection, difficulty will be experienced in using this technic for the accurate quantification of percentages of lymphocyte subsets in tissue sections.U     

急诊经皮腔内冠状动脉成型术(PTCA)无复流现象对预后的影响

目的探讨急诊PTCA中无复流现象的临床意义。方法回顾性分析2001年1月至2002年1月就诊于同仁医院的53例(男37例,女16例,年龄35～78岁)初发急性心肌梗死(AMI)行急诊PTCA的患者。病例被分为无复流组(18例,TIMI≤2级)和PTCA后冠脉血流正常组(35例,TIMI3级),随访6个月,观察有无心脏性猝死和非致命性心脏事件。组间差别采用t检验或χ2检验。结果无复流组发生充血性心衰、恶性≤2级性心律失常、再发心绞痛、心脏性猝死高于冠脉血流正常组(P<0.05)。无复流组6个月后左室射血分数显著低于冠脉血流正常组(P<0.05)。结论无复流现象强烈提示AMI预后不良。但需进一步的大规模临床试验验证。     

湿疹Ⅰ号搽剂的制备工艺研究

目的　探讨湿疹I号搽剂的最佳制备工艺。方法　采用比较法和正交试验法 ,对湿疹Ⅰ号搽剂的制备工艺进行优选。结果　以蛇床子 8倍量 75 %乙醇浸泡 7d ,残渣与其余药材加 12倍量水、煎煮 2次、煎 6 0min为最佳。结论　该工艺可用于指导本制剂的生产     

心身Ⅰ号抗应激性溃疡的实验研究

本实验依据中医学:“形神若一”和情志致病理论,以束缚水浸法模拟情志刺激制做大鼠应激性溃汤的模型,并以具有理气、降逆、散结作用的中药复方心身Ⅰ号进行治疗反证,证实该模型气机紊乱的实质是神经内分泌调节系统和微循环的紊乱,心身Ⅰ号具有调节神经内分泌系统和微循环功能,起到心(情绪、心理)身(躯体)并治的作用。     

绞股蓝总甙复合国产1号避孕片对健康妇女脂类代谢及血压的影响

本研究将180名已婚、一孩、年龄33±5岁,需要使用避孕措施的健康妇女随机分成6组,即IUD组、绞股蓝总甙加IUD组、1号OC片组、40mg、60mg、80mg绞股蓝总甙分别与1号OC片复合的3个试验组.用双盲法给药,连续服用6个月观察脂类代谢和血压的变化.结果显示:以IUD组和1号OC片组的数据为参比值.服用不同剂量绞股蓝总甙复合口服避孕药后无升高TG和TC的趋势,有显著升高HDL-C作用和使APOA_1水平上升的趋势;亦存在降低收缩压的趋势,对舒张压无明显影响,但在服药后3个月时HDL-C水平曾发生暂时性下降.     

生命1号健脑强身及促骨生长的实验研究

采用水迷宫法、穿梭箱法、避暗法、跳台法观察了生命1号对小鼠学习、记忆、巩固的影响。灌胃给生命1号可改善东莨菪碱、樟柳碱致小鼠记忆获得障碍。生命1号能明显提高单核细胞吞噬功能，显著增加小鼠血清溶血素的含量，促进T淋巴细胞的转化。除此之外，还能明显地增加阳虚大鼠股骨的重量、长度、直径及皮质厚度；提高血清钙、磷水平；降低血清碱性磷酸酶的含量，具有促进骨骼生长的作用。     

天津市和平区2000～2004年食品卫生投诉案件分析

目的 了解本地区食品卫生存在的问题及变化趋势，为卫生监督管理提供依据。方法对天津市和平区2000～2004年1058件食品卫生投诉案件进行分析。结果投诉案件逐年递增，内容集中在无卫生许可证(以下简称无证)经营、食品中异物、环境卫生、食品变质(异味)几种情况。此外，保健品、饮品、食品超市、学生用餐方面的食品卫生问题，已成为广大群众关注的新焦点。结论群众对食品卫生的投诉，反映了当前存在的问题，提示卫生管理部门今后须加强相应的管理。