Fluoro-Ruby labeling prior to an amphetamine neurotoxic insult shows a definitive massive loss of dopaminergic terminals and axons in the caudate-putamen

Fluoro-Ruby (FR) was injected into the substantia nigra (SNc) to label dopaminergic axons and terminals in the caudate putamen (CPu) of rats 7 days prior to a neurotoxic d-amphetamine (AMPH) exposure. Three days after AMPH exposure, a massive loss in the TH immunoreactive (TH⁺) axons and terminals was seen in the CPu. The FR-labeled (FR⁺) axons and terminals in the CPu were greatly diminished with those remaining being enlarged or swollen after AMPH. Fluoro-Jade C (FJ-C) labeling was used to verify AMPH-induced axonal and terminal degeneration. This study demonstrates that fluorescent anterograde tract tracers can be used to show the subsequent axonal and terminal degeneration after systemic exposures to toxins and provides direct evidence that CPu axons and terminals from SNc dopaminergic neurons can be destroyed after neurotoxic exposure to AMPH.     

肌激动器和FRⅡ型功能矫治器治疗安氏Ⅱ类1分类错的对比研究

目的:比较肌激动器和Fr&ankelⅡ(FRⅡ)型功能矫治器治疗安氏Ⅱ类1分类错的效果。方法:选择处于生长发育高峰期或高峰前期的患儿34例,其中12例用肌激动器矫治,22例用FRⅡ型矫治器矫治。所有患儿均为凸面型,上颌前突和(或)下颌后缩;ANB角\5b;磨牙及尖牙为远中关系。对比每个患儿矫治前后的模型和头侧位片并作统计学分析。结果:矫治结束后所有患儿磨牙和尖牙均达到或接近中性关系,ANB角减小至正常范围。使用 FRⅡ型矫治器组的患儿牙弓宽度比肌激动器组明显增加,而两组的基骨弓宽度无显著性差异。FRⅡ型矫治器使上切牙内收更显著,而肌激动器更能有效地控制下切牙的倾斜度。结论:两种功能矫治器都能有效地改善上下颌骨及牙弓的Ⅱ类关系。     

垂体腺瘤中FRα的表达与临床意义

目的检测垂体腺瘤中叶酸受体α（FRα）的表达情况,探讨FRα与垂体腺瘤相关临床指标的关系。方法运用冰冻切片免疫组化方法,检测124例垂体腺瘤中FRα的表达情况,分析其与患者性别、年龄、肿瘤径线、肿瘤侵袭性的关系。结果 FRα在NF腺瘤中的阳性率为73.8%（31/42）,在PRL腺瘤中的阳性率为9.7%（3/31）,在GH腺瘤中的阳性率为6.7%（2/30）,在ACTH腺瘤中的阳性率为9.5%（2/21）,FRα在NF腺瘤中的表达水平明显高于在其他病理类型垂体腺瘤中的表达（P〈0.05）。在垂体NF腺瘤中,FRα的表达与肿瘤的侵袭性有关（P〈0.05）,与性别、年龄、肿瘤大小无明显关系（P＞0.05）。在其他三种垂体腺瘤中,FRα与上述指标无明显关系。结论 FRα在垂体NF腺瘤中高表达,在其他病理类型的垂体腺瘤中低表达;FRα与垂体NF腺瘤的侵袭性相关。     

东芝TBA-120FR全自动生化分析仪与快速血糖仪检测血糖在糖尿病患者中的临床应用

目的探讨东芝TBA-120FR全自动生化分析仪与快速血糖仪检测血糖在糖尿病患者中的临床应用价值。方法选取2018年3月至2020年3月医院收治的90例糖尿病患者作为研究对象,全部患者均采用快速血糖仪(末梢血、静脉血)及东芝TBA-120FR全自动生化分析仪(静脉血)检测血糖,比较3种方法检测血糖的差异。结果全自动生化分析仪采集静脉血检测血糖的结果、快速血糖仪采集静脉血及末梢血检测血糖的结果比较,差异无统计学意义(P>0.05)。结论东芝TBA-120FR全自动生化分析仪与快速血糖仪均可用于监测糖尿病患者的血糖水平,且检测结果一致。     

A new antitumor antibiotic FR66973: clonogenic in vitro assessment of activity against human non-small cell lung carcinoma

Summary We have utilized a human tumor clonogenic assay (HTCA), as a disease-oriented drug screening model of new antitumor drugs, to test the antitumor activity of FR66973 and compared the activity with that of its analogous compound, mitomycin C. The overall in vitro response rate (defined as less than 50% survival of tumor colony forming units) for FR66973 against fresh tumor cells obtained from patients with non-small cell lung carcinoma (NSCLC) was 32%, 50% and 89% at 0.1, 1 and 10 g/ml, respectively, which was superior to that of mitomycin C at the corresponding concentration. Our data suggest that FR66973 is a promising new drug against NSCLC. If phase I toxicities are not prohibitive, FR66973 may also have good activity against NSCLC in clinical phase II trial.     

Ulcerative colitis: female fecundity before diagnosis, during disease, and after surgery compared with a population sample.

BACKGROUND & AIMS: Women with ulcerative colitis generally have normal fertility. The aim of this study was to compare patients' fecundability before and after restorative proctocolectomy with ileal pouch-anal anastomosis with the fecundability of the general population. METHODS: Historical follow-up was performed on 343 consecutive female patients aged 10.6-40.5 years at surgery and a reference population of 1200 women aged 25-40 years. A total of 290 (85%) patients and 661 (55%) women in the reference population agreed to participate in a structured telephone interview concerning reproductive behavior and waiting times to pregnancy. Cox regression and Kaplan-Meier plots were used for analysis. RESULTS: Surgery significantly reduced the ratio of patient to reference population fecundability, which decreased to 0.20 (P < 0.0001). Before diagnosis and from diagnosis until colectomy, the fecundability of the patients was similar to that of the reference population. CONCLUSIONS: Female patients with ulcerative colitis have normal fecundity before surgical treatment. Surgery severely reduces female fecundity. Information about this reduction in fecundity should be given before surgery, and if a woman has an unfulfilled wish for pregnancy after surgery, early referral to a gynecologist is recommended.     

Bradykinin B2 Receptor Antagonist FR173657 Ameliorates Small Bowel Ischemia–Reperfusion Injury in Dogs

Bradykinin mediates acute inflammation by increasing microvascular permeability, vasodilation, leukocyte migration and accumulation, and the production of arachidonic acid via phospholipase A₂ activation. Arachidonic acid metabolites, or eicosanoids, are potent modulators of biological functions, particularly inflammation. Bradykinin exerts its inflammatory effects via the bradykinin B₂ receptor. The aim of this study was to evaluate the effect of a bradykinin B₂ receptor antagonist, FR173657 (FR), on intestinal ischemia-reperfusion (I/R) injury. Twenty-eight mongrel dogs were divided into four groups (n = 7 per group). Group I underwent I/R alone, Group II underwent I/R and received FR treatment, Group III was sham operated, and Group IV was sham operated and received FR treatment. The FR treatment consisted of FR continuously from 30 min prior to ischemia to 2 hr after reperfusion. In the I/R procedure, the superior mesenteric artery (SMA) and vein were clamped for 2 hr and then released to permit reperfusion for 12 hr. The intramucosal pH (pH_i), SMA blood flow, and mucosal tissue blood flow were measured during the reperfusion period. The serum thromboxane B₂ and 6-keto-prostaglandin F₁ levels were determined, and tissue samples were examined histologically. Results showed that tissue blood flow, pH_i, and SMA blood flow after reperfusion were maintained in Group II in comparison with Group I. Histopathological examination showed less severe mucosal damage after reperfusion in Group II than in Group I. The serum thromboxane B₂ and 6-keto-prostagland in F₁ levels were significantly lower in Group II than in Group I (P < 0.05). We conclude that FR treatment appears to have clear protective effects on small bowel I/R injury by inhibiting the release of eicosanoids.     

安氏Ⅱ类1分类错双期治疗的X线头影测量分析

目的：探讨应用FR功能调节器＋MBT技术矫治青少年期安氏Ⅱ类1分类错牙合的临床治疗效果。方法：选择安氏Ⅱ类1分类的10.0～12.9岁错牙合患者36例,Ⅰ期治疗采用FR-I型功能调节器,Ⅱ期治疗采用MBT固定矫治,应用X线头影测量技术进行治疗前后软硬组织头影测量分析,对测量结果进行配对t检验。结果：矫治前后下颌骨的变化具有统计学意义,表现在SNB角、上下中切牙角的增大,ANB角、上切牙-上颌平面角的减小,前下面高增大,在正常范围内。结论：FR-I型功能调节器＋MBT技术矫治青少年安氏II类1分类错牙合患者可纠正错牙合畸形,有效改善面型,但要达到长久稳定的咬合关系,有些病例还是需要拔牙治疗。     

Development and applications of photo-triggered theranostic agents

Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.