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81.
Differential immunomodulatory properties of Bifidobacterium logum strains: relevance to probiotic selection and clinical applications 下载免费PDF全文
Modulation of host immunity is one of the proposed benefits of the consumption of probiotics. Nonetheless, comparative studies on the immunological properties that support the selection of strains of the same species for specific health benefits are limited. In this study, the ability of different strains of Bifidobacterium longum to induce cytokine production by peripheral blood mononuclear cells (PBMCs) has been evaluated. Live cells of all B. longum strains greatly stimulated regulatory cytokine interleukin (IL)-10 and proinflammatory cytokine tumour necrosis factor (TNF)-alpha production. Strains of the same species also induced specific cytokine patterns, suggesting that they could drive immune responses in different directions. The probiotic strain B. longum W11 stimulated strongly the production of T helper 1 (Th1) cytokines while B. longum NCIMB 8809 and BIF53 induced low levels of Th1 cytokines and high levels of IL-10. The effects of cell-surface components obtained by sonication of B. longum strains overall confirm the effects detected by stimulation of PBMCs with live cells, indicating that these components are important determinants of the immunomodulatory activity of B. longum. Genomic DNA of some strains stimulated the production of the Th1 and pro-inflammatory cytokines, interferon (IFN)-gamma and TNF-alpha, but not that of IL-10. None of the cell-free culture supernatants of the studied strains was able to induce TNF-alpha production, suggesting that the proinflammatory component of these strains is associated mainly with structural cell molecules. The results suggest that despite sharing certain features, some strains can perform a better functional role than others and their careful selection for therapeutic use is desirable. 相似文献
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目的:了解东莞市儿童支气管哮喘急性发作期的病原学分布特征,指导临床治疗。方法:选择东莞市太平人民医院2012年6月至2013年6月收治住院的支气管哮喘急性发作患儿共166例,根据年龄分为〈3岁婴幼儿组和≥3岁儿童组,分别检测病毒、支原体(MP)、衣原体(CP)及细菌等病原体分布情况。结果:166例患儿检出病毒、MP、CP、细菌感染任何一项阳性者共132例,占79.5%,其中检出病毒69例(41.6%)、CP 16例(9.6%)、MP 39例(23.5%),细菌培养阳性39例(23.5%)。结论:东莞市儿童哮喘急性发作与感染密切相关,病毒感染最多见,MP、细菌感染亦较常见。 相似文献
84.
目的:探讨难治性肺炎难治的原因,为临床治疗提供抗感染思路与技巧。方法:对成功参与治疗的典型难治性肺炎病例进行回顾性分析,从病原体、宿主、药物等环节分析难治的原因,总结对策。结果:对于难治性肺炎,应明确其难治的原因,并采取相应措施,制订个体化抗感染方案,可提高抗感染成功率。结论:临床药师应全面提升自身的医学知识水平,掌握扎实的药学专业知识,具备分析临床信息并以药学专业优势制定相应治疗策略的能力,以提高临床用药安全性及救治成功率。 相似文献
85.
《International journal of antimicrobial agents》2014,43(6):485-496
In the field of antibiotherapy, intracellular infections remain difficult to eradicate mainly due to the poor intracellular penetration of most of the commonly used antibiotics. Bacteria have quickly understood that their intracellular localisation allows them to be protected from the host immune system, but also from the action of antimicrobial agents. In addition, in most cases pathogens nestle in professional phagocytic cells, and can even use them as a ‘Trojan horse’ to induce a secondary site of infection thereby causing persistent or recurrent infections. Thus, new strategies had to be considered in order to counteract these problems. Amongst them, nanocarriers loaded with antibiotics represent a promising approach. Nowadays, it is possible to encapsulate, incorporate or even conjugate biologically active molecules into different families of nanocarriers such as liposomes or nanoparticles in order to deliver antibiotics intracellularly and hence to treat infections. This review gives an overview of the variety of nanocarriers developed to deliver antibiotics directly into infected cells. 相似文献
86.
目的 探讨前降钙素(PCT)在肾移植术后患者肺部细菌感染中的诊断价值.方法 61例肾移植术后肺部感染患者在入院后完善相关检查,同时检测PCT水平.血清PCT水平的检测采用免疫发光分析法.结果 将各炎症指标绘制成ROC曲线,显示当PCT水平≥0.5ng/ml时在诊断肾移植术后肺部细菌感染时敏感性和特异性最高,分别为81%和66%;且PCT阳性患者细菌感染的发生率明显高于PCT阴性患者(P<0.01).结论 PCT水平≥0.5 ng/ml时提示肺部细菌感染可能性越大,是抗生素的适用指征. 相似文献
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摘 要 目的:对我院重症医学科近3年细菌检出结果和耐药率进行统计分析,为临床抗感染治疗提供参考。方法:收集2015~2017年粤北人民医院重症医学科所有检出细菌分离的菌株及药敏结果,统计分析该区域细菌流行趋势及耐药率变迁。结果:近3年共检出1 399株细菌,其中革兰阴性菌株1 021株(72.98%),革兰阳性菌378株(27.02%),多重耐药菌448株(32.02%)。鲍曼不动杆菌是检出率最高的细菌(25.16%),对碳青霉烯耐药严峻(>73.31%);肺炎克雷伯菌属(17.30%)近3年检出率呈上升趋势,对酶抑制药复合制剂和碳青霉烯类药物耐药率逐年增高;铜绿假单胞菌检出率排第3,检出率呈下降趋势;凝固酶阴性葡萄球菌、金黄色葡萄球菌和肺炎链球菌是检出率最高的革兰阳性菌,其中肺炎链球菌近3年检出趋势逐渐升高,3种细菌对青霉素耐药严重(>90%),暂未发现万古霉素、替考拉宁和利奈唑胺耐药菌。结论:我院重症医学科检出细菌以革兰阴性菌为主,抗菌药物耐药情况严峻,应结合本区域细菌流行趋势和耐药情况合理选用抗菌药物。 相似文献
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90.
《Revista de gastroenterologia de Mexico》2014,79(2):96-134
BackgroundPost-infectious irritable bowel syndrome (PI-IBS) prevalence, small intestinal bacterial overgrowth (SIBO), altered microbiota, low-grade inflammation, and antibiotic therapy in IBS are all controversial issues.AimsTo conduct an evidence-based review of these factors.MethodsA review of the literature was carried out up to July 2012, with the inclusion of additional articles as far as August 2013, all of which were analyzed through the Oxford Centre for Evidence-Based Medicine (OCEBM) system.Results1. There is greater SIBO probability in IBS when breath tests are performed, but prevalence varies widely (2-84%). 2. The gut microbiota in individuals with IBS is different from that in healthy subjects, but a common characteristic present in all the patients has not been established. 3. The incidence and prevalence of PI-IBS varies from 9-10% and 3-17%, respectively, and the latter decreases over time. Bacterial etiology is the most frequent but post-viral and parasitic cases have been reported. 4. A sub-group of patients has increased enterochromaffin cells, intraepithelial lymphocytes, and mast cells in the intestinal mucosa, but no differences between PI-IBS and non-PI-IBS have been determined. 5. Methanogenic microbiota has been associated with IBS with constipation. 6. Rifaximin at doses of 400 mg TID/10 days or 550 mg TID/14 days is effective treatment for the majority of overall symptoms and abdominal bloating in IBS. Retreatment effectiveness appears to be similar to that of the first cycle.ConclusionsFurther studies are required to determine the nature of the gut microbiota in IBS and the differences in low-grade inflammation between PI-IBS and non-PI-IBS. Rifaximin has shown itself to be effective treatment for IBS, regardless of prior factors. 相似文献