Trans-scleral application of a semiconductor diode laser

We used a diode laser with an output power of 1 W through a fiberoptic light pipe (200 microns diameter) to deliver laser energy through the sclera of pigmented rabbits. Ciliary body destruction occurred with energy levels of 300-400 mW and exposure time of 0.5 sec. Retinal photocoagulation was achieved with energy levels of 200-500 mW in 0.5 sec. Histologic examination of acute lesions demonstrated thermal destruction of ciliary body processes and retina. Chorioretinal scar formation was observed clinically and histologically within 2-3 weeks. Our data indicate that the transscleral diode laser may be used for destruction of the ciliary body processes or peripheral retinal coagulation in pigmented eyes.     

质粒pGH/BL_(21)在大肠杆菌中表达条件的研究

目的　寻找质粒 pGH/BL2 1在大肠杆菌中的最佳表达条件。方法　当它在大肠杆菌中表达时 ,考查不同IPTG诱导浓度及不同的诱导时间对表达产物的影响 ,表达产物的量以SDS -PAGE电泳方法分析。结果　该基因的最佳表达条件为 :IPTG诱导浓度 1.0mmol/L ,诱导时间 4h。结论　IPTG对不同的基因在不同的表达体系中需不同的诱导条件 ,只有找到它的最佳诱导条件才能保证终产物的最大产率     

黄芪、小柴胡冲剂联合辅酶Q10治疗病毒性心肌炎

目的观察黄芪、小柴胡冲剂联合辅酶Q10治疗病毒性心肌炎(VMC)的临床疗效.方法治疗组(30例)应用黄芪注射液、小柴胡冲剂和辅酶Q10;对照组(30例)用GIK极化液、辅酶Q10和维生素C,两组患者无禁忌证者均给予β-受体阻滞剂,钙拮抗剂和对症治疗.两组疗程均为3个月.结果治疗组临床症状和心律失常总有效率分别为73.3%和86.6%,高于对照组的43.3%和63.3%(p＜0.05).结论黄芪、小柴胡冲剂联合辅酶Q10对VMC有较好疗效,且无明显不良反应.     

抗精神病药物治疗引起患者体质量增加及其相关因素分析

目的　探讨抗精神病药(APD)引起患者体质量增加及其相关因素。方法　对6 7例首次住院单用APD治疗的精神分裂症患者进行住院及出院后4个月的随访评估。结果　各时点体质量增加与GI评分无相关性,在出院时与BPRS、SAPS减分值有相关性,而随访期与SANS减分值有相关意义。逐步回归分析显示,在α=0 .0 5水平上,进入回归方程的因素依次为:APD品种,最大服药剂量与服药时间的积,阴性症状,病前1a最佳功能水平。结论　APD治疗中的体质量增加是与疗效无关的药物不良反应,受药物、精神症状及综合社会心理因素等方面的影响,而饮食与活动的中介作用不应低估。控制体质量增加有重要的医学及社会意义。     

Buspirone does not produce a 5-HT1A-mediated decrease in temperature in man

Summary Buspirone, a putative serotonin (5-HT)_1A partial agonist, did not produce hypothermia in 17 normal volunteers in a placebo controlled, single blind study. Thus, buspirone may be a weaker agonist at those 5-HT_1A receptors which mediate hypothermia compared to ipsapirone or gepirone, two other 5-HT_1A partial agonists which have been reported to produce hypothermia by a 5-HT_1A-mediated mechanism.     

Haemodynamics as a determinant of the pharmacokinetics of and the plasma catecholamine responses to isoprenaline

Summary The total body clearance and fractional extraction of isoprenaline (ISO) have been determined, and the relation between these parameters and cardiac output established. Whether desipramine, an inhibitor of neuronal uptake, altered the plasma catecholamine response to ISO was also investigated.Seven healthy subjects were given i.v., infusions of ISO in two, consecutive 25-min periods, at constant dose rates of 31–43 and 80–124 pmol·kg^–1·min^–1, respectively. The total-body (ER), pulmonary (ER_p) and forearm (ER_f) fractional extractions and the total body clearance (CL) of ISO were obtained from measurements of cardiac output and the steady-state ISO concentration in mixed central venous, arterial and forearm venous plasma.ISO-induced increases in cardiac output resulted in increases in CL, decreases in ER and no consistent change in ER_f. ER_p did not differ from zero. ISO also produced a dose-dependent increase in the mixed venous plasma concentrations of noradrenaline and 3,4-dihydroxyphenylglycol (DOPEG), and a decrease in that of adrenaline. Pretreatment with desipramine did not alter any of the pharmacokinetic parameters of ISO. Desipramine, however, reduced the mixed venous baseline plasma levels of noradrenaline (47%) and DOPEG (40%), and tended to reduce that of adrenaline (34%). It enhanced the plasma noradrenaline response 2.4-fold, abolished the plasma DOPEG response and did not alter the plasma adrenaline response to ISO.Hence, owing to its haemodynamic effects, ISO modifies its own pharmacokinetics which involve non-neuronal removal processes only. The increased DOPEG in plasma resulting from the ISO-induced increase in noradrenaline release was presynaptic in origin. Desipramine appears to reduce sympathetic activity. The enhancement by desipramine of the ISO-induced increase in plasma noradrenaline points towards recapture by neuronal uptake of at least 58% of the noradrenaline released in response to ISO.     

The complex enterprise of modelling prenatal exposure to cigarettes: what is ‘enough’?

While there is a burgeoning body of research linking smoking during pregnancy to problem behaviour in offspring, a major criticism of this work has been the crude measurement of exposure in these studies (e.g. retrospective, self-reported only) that could lead to biased estimates. To address this issue, we used a pregnancy cohort with repeated prospective measures of exposure as well as biological assays to generate estimates of exposure patterns using a range of modelling techniques. In this paper we report on the analytical approaches we have developed, including patterns of exposure over time and best-estimate approaches that combine self-report and cotinine measures, and compare their predictive value in relation to different dimensions of fetal growth as a first step towards examining the utility of greater precision of exposure measurement.
Surprisingly, in this sample the more complex assessments of exposure, including biological measures, generally did not perform better than simple indicators of exposure based on repeated self-report measures, with one exception: a combined self-report cotinine 'best estimate' of third trimester exposure was uniquely associated with lower brain : body ratio. Further study is needed using more sophisticated cotinine assays and testing prediction of a range of outcomes to ascertain whether these findings represent true differences or are specific to the sample, methods and outcomes used. Such research will inform the development of guidelines for adequate exposure characterisation in developmental studies.     

体表面积、体质量指数对老年骨质疏松的影响

目的探讨体表面积（BS）、体质量、体质量指数（BMI）对老年人骨密度（BMD）的影响。方法用双能X线骨密度仪测定1794例老年人腰椎、髋关节的BMD值，并对其与BS等指标进行相关回归分析。结果BS大的老年人其BMD大于BS小者，有显著性差异。在各种指标与BMD的相关性比较中，BS与BMD的相关性最好，其后依次为：体质量、身高、OSTA得分（亚洲人自我骨质疏松筛查工具）、BMI。结论BS比BMI等指标更能反映出人体体型对老年人BMD的影响。     

Pathological and biochemical studies on a case of Pick disease with severe white matter atrophy

We report on a male patient with Pick disease who had shown severe white matter atrophy and dilatation of the lateral ventricle in the frontal lobe from an early stage. Upon admission to our hospital 2 years after disease onset, the patient showed apathy, and MRI revealed severe atrophy of the cortex and white matter of the frontal lobe. He died at age 74, 11 years after disease onset. Autopsy revealed severe atrophy of the frontal and temporal lobes, severe loss of white matter in the frontal lobe, dilatation of the lateral ventricles, and cortical thinning. Histopathological examination showed severe loss of myelinated fibers in the frontal white matter and severe neuronal loss with gliosis in the frontal and temporal cortices. Many Pick bodies were seen. Our patient had a rare case of Pick disease predominantly affecting the frontal lobe with severe involvement of the white matter from an early stage. This case suggests that myelinated fibers in the white matter as well as cerebral neurons are primarily affected in Pick disease.