阿托伐他汀对兔心肌缺血再灌注损伤的实验研究

目的:研究阿托伐他汀(ATV)对心肌缺血再灌注损伤的影响及两种ATP依赖的K+(K+-ATP)通道的作用.方法:将健康家兔随机分为对照组、ATv组、ATV+5-HD(A1组)、ATV+HMR1883(A2组).进行30 min局部缺血和30 min再灌注.实验中持续监测左室舒张末期压(LVEDP)、左心室压最大变化速率(±dp/dtmax)的变化,再灌注结束后用伊文蓝和TTC染色法确定缺血和梗死心肌范同.结果:与对照组相比,ATV组及A2组的梗死面积显著减少(P＜0.05),各项心功能指标在再灌注30 min后明显改善(P＜0.05);这种效应能够被5-HD所消除,A1组与对照组相比无显著性差异(P＞0.05).结论:ATV对心肌缺血再灌注损伤有早期保护效应.且这种作用可能与心肌线粒体KATP通道有关系.     

高碘酸银钾合成宏观反应动力学

建立了在强碱、强氧化条件下,由硝酸银合成高碘酸银钾的液固配合反应宏观动力学模型,推导了两种条件下的动力学方程式,并经实验验证。高碘酸根的初始浓度c0AL≤55.6 mmol/L时,反应为传质控制,动力学表达式为［1-(1-xB)1/2］·(1+110.7x1/2.04B)=t;当55.6 mmol/L≤c0AL≤139 mmol/L时,反应处于传质和反应阻力共存区,动力学表达式为100.6［1-(1-xB)1/2］·［1-(1-xB)1/1.51］=t。与实际数据相比较,两种情况下的动力学方程式均有良好的     

硝苯地平缓释片配伍氯沙坦钾片治疗血液透析患者高血压的应用疗效

目的 研究硝苯地平缓释片配伍氯沙坦钾片治疗血液透析患者高血压应用效果.方法 选取2019年2月至2021年2月在我院接受治疗的64例血液透析高血压患者,根据入院随机编号将其分为研究组和对照组,对照组给予氯沙坦钾片治疗,研究组在氯沙坦钾片基础上联合使用硝苯地平缓释片,观察两组患者治疗前后血压变化.结果 治疗后,研究组收缩...     

心脏电压依赖性钾通道的研究进展

通过科技的进步,随着分子生物学技术的发展,引进的结构分析,电子和重组DNA技术方法,克隆了多种钾离子通道基因,使其能够更多的了解钾离子通道功能和遗传结构,以及基因结构和渠道动态和渠道之间的关系。本文仅对第一类K+通道即电压依赖性K+通道的最新研究进展简略综述如下。     

Activation of PI3-K/Akt pathway for thermal preconditioning to protect cultured cerebellar granule neurons against low potassium-induced apoptosis

AIM: This study was designed to investigate whether the activation of the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway is required for thermal preconditioning to protect rat cerebellar granule neurons (CGN) against apoptosis induced by low potassium, and to explore the possibility of a link between the upregulated heat shock protein (HSP)70 expression and Akt activation in the acquisition of neuroprotection induced by thermal preconditioning. METHODS: CGN cultured for 8 d in vitro were switched to 5K medium for 24 h after thermal preconditioning (TP; 43.5 degree for 90 min, then 37 degree for 1 h). To study the role of the PI3-K/Akt pathway, a PI3-K inhibitor, LY294002 (20 micromol/L) was added into the cultures 1 h before TP. 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay and fluorescein diacetate staining were used to determine cell viability. Hoechst 33258 staining and agar gel electrophoresis were used to test the morphological and biological characters of CGN. Western blot analysis was employed to detect the levels of phospho-Akt, phospho-glycogen synthase kinase 3beta (GSK3beta) Akt, GSK3beta, and HSP70. RESULTS: TP protected CGN against apoptosis induced by low potassium. LY294002 inhibited the neuroprotective effect on CGN induced by TP. TP induced a robust activation of Akt and the inactivation of GSK3beta via PI3-K. Furthermore, the activation of the PI3-K/Akt pathway by TP persisted for 24 h in the 5K cultures. LY294002 (20 micromol/L) failed to inhibit the upregulated HSP70 expression induced by TP. CONCLUSION: The activation of the PI3-K/Akt pathway is required for TP to protect CGN against apoptosis induced by low potassium, but the neuroprotective effect by Akt activation is not mediated through the downstream induction of HSP70 expression.     

G protein-coupled inwardly rectifying potassium channels in dorsal root ganglion neurons

AIM: G protein-coupled inwardly rectifying potassium channels (GIRK) are important for neuronal signaling and membrane excitability. In the present study, we intend to find whether GIRK channels express functionally in adult rat dorsal root ganglion (DRG) neurons. METHODS: We used RT-PCR to detect mRNA for 4 subunits of GIRK in the adult DRG. The whole-cell patch clamp recording was used to confirm GIRK channels functionally expressed. RESULTS: The mRNA for the 4 subunits of GIRK were detected in the adult DRG. GTPgammaS enhanced inwardly rectifying potassium (K+) currents of the DRG neurons, while Ba2+ inhibited such currents. Furthermore, the GIRK channels were shown to be coupled to the GABA(B) receptor, a member of the G protein-coupled receptor family, as baclofen increased the inwardly rectifying K+ currents. CONCLUSION: GIRK channels are expressed and functionally coupled with GABA(B) receptors in adult rat DRG neurons.     

氯沙坦钾片对高血压左心室肥厚患者的治疗效果及对血清中IGF-1的影响

目的 观察氯沙坦钾片对高血压左心室肥厚(LVH)患者的治疗作用及对血清中胰岛素样生长因子(IGF)-1的影响,探讨其临床意义.方法 选取180例高血压LVH患者,随机分为氯沙坦钾片组和硝苯地平组各90例,观察其治疗前、后舒张末期室间隔厚度(IVST)、舒张末期左室后壁厚度(LVPWT)和左心室重量指数(LVMI)改变,同时于治疗前、治疗后观察血清中IGF-1的变化.结果 两组治疗后IVST、LVPWT和LVMI均明显减小,但是氯沙坦钾片组减少值明显高于硝苯地平组(P＜0.05).两组治疗后血清中IGF-1明显低于治疗前,但氯沙坦钾片组治疗前、后IGF-1的减少值明显高于硝苯地平组(P＜0.05).结论 氯沙坦钾片对高血压LVH患者的治疗作用明显,且对血清中IGF-1的表达有重要的降低作用.     

174例急性脑梗死患者早期血钾含量变化分析

为探讨脑血管病的综合治疗 ,合理补钾 ,维持电解质平衡 ,我们对 174例急性脑梗死患者早期进行了血钾检测 ,现将结果报告如下。1　资料与方法1·1　临床资料　病例选自 1996年 11月至 1998年 5月本院收治的经头颅ＣＴ或ＭＲＩ确诊的急性脑梗死患者 ,共 174例 ,其中男 90例 ,女 84例 ;年龄 2 7～ 86岁 ,平均 ( 54± 3 0 )岁。梗死部位 :大脑 144例 ,小脑12例 ,脑干 18例 ;颈动脉供血区和椎基底动脉供血区同时发生 2 6例。在 144例大脑半球梗死中 ,梗死病灶位于基底神经节区 66例 ,多发性 4 2例 ,丘脑受损 36例。本组 174例脑梗死患者死亡 10例…     

小剂量激素联合他克莫司胶囊对局灶节段性肾小球硬化并发早期肾衰竭的影响

目的:分析局灶节段性肾小球硬化(FSGS)并早期肾衰竭应用小剂量激素与他克莫司胶囊联合治疗对血液相关指标的影响。方法:选取局灶节段性肾小球硬化并早期肾衰竭64例患者,按照随机数字表法分成两组,每组32例,研究组予以小剂量激素与他克莫司胶囊联合治疗,对照组单独予以小剂量激素治疗,分析两组血液相关指标情况。结果:治疗前,两组血压(舒张压及收缩压)、血糖及血钾指标无统计学差异(P>0.05),治疗后,各指标均降低(P<0.05),且研究组收缩压、舒张压及血钾明显比对照组低(P<0.05);两组治疗前24 h尿蛋白定量(U-TP)及血肌酐(SCr)指标无统计学差异(P>0.05),治疗后均降低(P<0.05),研究组降低幅度比对照组大(P<0.05)。结论:对局灶节段性肾小球硬化并早期肾衰竭患者应用小剂量激素与他克莫司胶囊联合方案,能有效改善对血糖、血压及血钾水平的不良影响,同时保护肾功能。