Self-reported denture hygiene habits and oral tissue conditions of complete denture wearers

The aim of the study was to examine the influence of denture hygiene on the development and intensity of denture stomatitis and traumatic ulcers and their relationship to age, gender, educational level, smoking status, age of the denture, denture hygiene habits and denture-wearing behavior. The study population comprised 310 complete denture (CD) wearers (159 males and 151 females) aged 60–85 living in Kırıkkale, Turkey. Denture hygiene habits were assessed and recorded, and any relationships between denture hygiene habits and the condition of oral tissue were assessed. The majority (48.4%) of participants reported cleaning their dentures with a toothbrush and toothpaste and 45.2% with a toothbrush only. Less than half (44.8%) removed their dentures overnight. Stomatitis was observed in 35.8% and traumatic ulcers in 29% of patients. A positive relationship was observed between poor denture hygiene habits and the presence of denture-related stomatitis and traumatic ulcers. The degree of denture hygiene was significantly associated with age, sex, education, general health status, smoking status, self-perception of halitosis, overnight denture removal and denture immersion habits.     

Association of interleukin-1beta polymorphism with recurrent aphthous stomatitis in Brazilian individuals

BACKGROUND: Recurrent aphthous stomatitis (RAS) is characterized by recurrent episodes of oral ulceration in an otherwise healthy individual. Some reports in the literature indicate that RAS may have immunological, psychological, genetic and microbiological bases. The purpose of the present study was to investigate the possible association between interleukin-1beta (IL-1beta) +3954 (C/T) genetic polymorphism and RAS in a sample of Brazilian patients. SUBJECTS AND METHODS: Sixty-two consecutive subjects affected by minor and major forms of RAS and 62 healthy volunteers were genotyped at IL-1beta (+3954). The chi-squared test was used for statistical analysis. RESULTS: A significant increase in the high production of IL-1beta genotype CT was observed in the group with RAS (P = 0.01). After stratifying RAS patients according to the mean number of lesions per episode, a significant difference was only observed between patients with >or=3 lesions in each episode and control. CONCLUSION: There is an increased frequency of polymorphism associated with high IL-1beta production in RAS patients.     

CD16+ monocytes exposed to HIV promote highly efficient viral replication upon differentiation into macrophages and interaction with T cells

The CD16+ subset of monocytes is dramatically expanded in peripheral blood during progression to AIDS, but its contribution to HIV pathogenesis is unknown. Here, we demonstrate that CD16+ but not CD16- monocytes promote high levels of HIV replication upon differentiation into macrophages and interaction with T cells. Conjugates formed between CD16+ monocyte-derived macrophages and T cells are major sites of viral replication. Furthermore, similar monocyte-T cell conjugates detected in peripheral blood of HIV-infected patients harbor HIV DNA. Thus, expansion of CD16+ monocytes during HIV infection and their subsequent recruitment into tissues such as lymph nodes, brain, and intestine may contribute to HIV dissemination and establishment of productive infection in T cells.     

综合疗法治疗小儿疱疹性口炎临床观察

目的：观察健康宣教、家庭护理及中西医结合综合治疗小儿疱疹性口炎的临床疗效。方法：将笔者所在科室2012年2月-11月诊断为疱疹性口炎的120例患儿随机分为治疗组和对照组,各60例,对照组予以利巴韦林气雾剂局部应用,治疗组在对照组治疗基础上予健康宣教、家庭护理及口服口炎清颗粒治疗,观察两组疗效。结果：治疗组总有效率95%,对照组总有效率78%,治疗组疗效优于对照组,差异有统计学意义（P〈0.05）;两组症状、体征消失时间相比,观察症状、体征消失时间明显短于对照组,差异具有统计学意义（P〈0.05）。两组患者在治疗过程中均未见不良反应。结论：综合疗法治疗小儿疱疹性口炎见效快,疗程短,值得临床推广。     

Iseganan HCl: a novel antimicrobial agent

Iseganan HCl is an antimicrobial peptide under development for the prevention of oral mucositis, a severe consequence of some chemotherapy and radiation therapy regimens. Several attributes of iseganan make it an optimal candidate for study in this clinical situation where both local and systemic host defenses may be impaired. These include broad spectrum and rapid bactericidal activity, a lack of observed resistance and cross-resistance and stability in biological fluids. Clinical trials of patients receiving stomatotoxic chemotherapy followed by a haematopoietic stem cell transplant show iseganan reduces the occurrence of oral mucositis and ameliorates sequelae such as mouth pain, throat pain and difficulty swallowing. Iseganan is well-tolerated, which is partly attributable to a lack of systemic absorption following topical oral administration. Other promising areas of investigation include topical oral application for the prevention of ventilator-associated pneumonia and nebulisation for treatment of chronic lung infection in patients with cystic fibrosis. Future studies will expand on the role of iseganan as a novel antimicrobial.     

The association between socioeconomic status, oral hygiene practice, denture stomatitis and oral status in elderly people living different residential homes

Oral hygiene practices and denture status of elderly people living in residential homes are different depending on the type of residential homes. In this study the elderly people living in three different residential homes were investigated for this purpose. A total of 269 subjects, 119 males (mean age 73.9 ± 8.8) and 150 females (mean age 78.5 ± 7.2) were involved in this study. All subjects were interviewed and clinically examined. Age, sex, educational status, financial status, general health, dental visiting, overnight denture wearing, brushing habits and frequency were recorded using a structured questionnaire. The prevalence of edentulism, the presence and type of dental prostheses, denture cleanliness and the presence of denture stomatitis were evaluated. Forty-one percent of subjects were non-educated. Fifty-one percent of subjects had low income. The majority of people (66.6%) were edentulous. Among the edentulous subjects 32% had full denture and 16% had no denture. Forty three percent of the subjects reported cleaning their dentures with water and 40% with a toothbrush only. Denture hygiene was good for 14% of the subjects. Denture stomatitis was observed in 44% of the subjects wearing dentures. There was statistically significant difference between residential homes and educational status, level of income, dental visiting, denture status, brushing methods and brushing frequency (p < 0.001). A positive relationship was observed between poor denture hygiene habits and the presence of denture-related stomatitis. The most important need within the residents of the residential home was the enhancement of oral care social insurance.     

真菌性咽喉炎19例临床分析

目的研究真菌性咽喉炎的临床特征、诊断及治疗方法。方法回顾性分析2006年5月-2009年5月我科诊治的的19例真菌性咽喉炎的临床资料,研究其伴发症状、体征、辅助检验,采用喉镜吸净分泌物,制霉菌素、碳酸氢钠等含漱2到3周加雾化的治疗方法,分析其疗效。结果 18例临床治愈。症状多于治疗5d后明显好转。2周后复诊,声嘶明显减轻。3周后无自觉症状,1例3月后复发,再次治疗后治愈。1例咽痛明显、双侧颌下淋巴肿大患者确诊为全身多处深层组织真菌感染,未愈。结论真菌性咽喉炎以往发病率较低,易被误诊。本病致病因素主要为各种原因所致的菌群失调、免疫力下降及局部自洁功能下降分泌物潴留。真菌性咽喉炎大多数患者局部治疗即可,局部治疗5d无效者,可给予全身抗真菌药物治疗,疗效较好。     

Systemic immune inflammation index in patients with recurrent aphthous stomatitis

ObjectivesRecurrent Aphthous Stomatitis (RAS) a chronic idiopathic oral mucosal disease. But yet the etiology and pathogenesis of RAS are not exactly known, it is thought that inflammation play an important role in the pathogenesis. The aim of this study is to demonstrate the role of systemic inflammation among the possible etiological factors of RAS and to find the possible diagnostic correlation between Systemic Immune Inflammation Index (SII).MethodsPatients who were consulted the otolaryngology outpatient clinic and diagnosed with RAS between 2019–2021 were retrospectively analyzed. Neutrophil/Lymphocyte Ratio (NLR), Platelet/Lymphocyte Ratio (PLR) and SII values were calculated based on the results of complete blood count. Demographic and hematological parameters between control and RAS groups were compared. The statistical significance level was considered as <0.05.ResultsThere was no statistically significant difference between the control and RAS groups in terms of sex and age distributions (p = 0.566 and p = 0.173, respectively). SII, NLR and PLR values were significantly higher in the RAS group compared to the controls (p < 0.001, p < 0.001 and p = 0.001, respectively). A very strong correlation between SII and NLR, moderately strong correlation between SII and PLR and moderate correlation between NLR and PLR values were detected (respectively ρ: 0.813, 0.719, 0.532; p-values <0.001).ConclusionSII, NLR and PLR has significantly higher levels in the RAS group compared to the control group, that it supports the role of systemic inflammation in the etiopathogenesis of RAS. In addition, the results show that SII is a valuable marker for inflammation.Level of evidence4.     

Consensus summary report for CEPI/BC March 12–13, 2020 meeting: Assessment of risk of disease enhancement with COVID-19 vaccines

A novel coronavirus (CoV), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in late 2019 in Wuhan, China and has since spread as a global pandemic. Safe and effective vaccines are thus urgently needed to reduce the significant morbidity and mortality of Coronavirus Disease 2019 (COVID-19) disease and ease the major economic impact. There has been an unprecedented rapid response by vaccine developers with now over one hundred vaccine candidates in development and at least six having reached clinical trials. However, a major challenge during rapid development is to avoid safety issues both by thoughtful vaccine design and by thorough evaluation in a timely manner. A syndrome of “disease enhancement” has been reported in the past for a few viral vaccines where those immunized suffered increased severity or death when they later encountered the virus or were found to have an increased frequency of infection. Animal models allowed scientists to determine the underlying mechanism for the former in the case of Respiratory syncytial virus (RSV) vaccine and have been utilized to design and screen new RSV vaccine candidates. Because some Middle East respiratory syndrome (MERS) and SARS-CoV-1 vaccines have shown evidence of disease enhancement in some animal models, this is a particular concern for SARS-CoV-2 vaccines. To address this challenge, the Coalition for Epidemic Preparedness Innovations (CEPI) and the Brighton Collaboration (BC) Safety Platform for Emergency vACcines (SPEAC) convened a scientific working meeting on March 12 and 13, 2020 of experts in the field of vaccine immunology and coronaviruses to consider what vaccine designs could reduce safety concerns and how animal models and immunological assessments in early clinical trials can help to assess the risk. This report summarizes the evidence presented and provides considerations for safety assessment of COVID-19 vaccine candidates in accelerated vaccine development.     

蒋士卿治疗放射性口腔炎的临床经验

放射性口腔炎是口腔和头颈部肿瘤放疗过程中常见的并发症,西医目前尚无有效治疗手段。根据放射性口腔炎的临床表现,蒋士卿教授认为放射线属于“火毒”之邪,火毒蕴结、气阴两伤是放射性口腔炎的基本病机。随着射线剂量的累加,邪热渐盛,正气渐虚,脾气渐伤,久病及肾,疾病呈阶段性。发病初期热结上焦、火毒灼津,中期热伏中焦、脾虚热结,后期热伤下焦、虚火上炎,火邪贯穿于放射性口腔炎临床表现的始终,因此其根据放疗不同阶段口腔炎症特点标本兼顾,辨证诊治,分别运用泻火解毒法、泻火补土法、补土伏火法,临床治疗效果显著。