量化健康教育在脑卒中患者健康教育中的应用

目的探讨量化健康教育在脑卒中住院患者健康教育中的效果。方法将200例脑卒中住院患者,随机分为两组,实验组108例患者采用量化健康教育的方法,对照组92例患者采用随机健康教育的方法。结果评价两组患者在出院当天对疾病知识掌握情况,主动咨询相关知识情况,对健康教育满意度,实验组不同文化程度患者相关知识掌握情况进行比较,差异有统计学意义（P〈0．05）。结论量化健康教育不仅加快疾病的恢复,使患者了解有关疾病的很多知识,增强了患者的参与意识,提高了患者自我保健与自我护理能力,防止疾病的复发,而且提高了护理人员在患者心目中的威信,也预防了护理中潜在问题的发生。     

聚乙二醇干扰素α-2a成功治疗1例乙型肝炎多重耐药患者

患者,男性,47岁,国家公务员,有乙型肝炎(乙肝)家族史。2003年首次发现乙肝病毒(HBV)阳性,当时肝功能正常。2006年初查丙氨酸氨基转移酶(ALT)152IU/L,曾用甘利欣等保肝治疗,ALT反复波动。2007年2月查ALT65IU/L,HBeAg(+),HBV DNA 6.8×107拷贝/ml,开始恩替卡韦(博路定)抗病毒治疗。治疗半年HBV DNA未转     

量化考核绩效管理在优质护理中的应用

目的探讨护理绩效分配制度在优质护理中的应用,提高护理人员工作积极性,提高医院护理工作质量。方法在优质护理示范病区中应用量化考核绩效管理制度,比较实施前后患者及医生对护士工作的满意度、护理质量及护理差错发生情况。结果实施后护理质量得分分别为（96．38±0．91）,（98．26±0．90）,（97．39±0．93）,（98．69±0．96）,（97．28±0．74）,（98．57±0．98）,（99．26±0．93）分,均高于实施前的（92．36±0．72）,（90．38±0．75）,（89．26±0．76）,（91．57±0．78）,（89．93±0．76）,（91．23±0．78）,（91．38±0．79）分,差异均有统计学意义（t分别为36．647,90．990,82．833,10．910,636．529,60．461,13．177;P〈0．05）;实施后患者对护理各项工作满意度较实施前提高,差异均有统计学意义（t分别为27．750,22．733,49．430,41．483,12．926,8．980,8．517,7．806,12．920;P〈0．05）;实施后医生对护理各项工作满意度较实施前提高,差异均有统计学意义（t分别为27．336,12．975,14．344,18．237,25．745,13．100;P〈0．05）。实施后护理差错发生减少,差异有统计学意义（X2=0．845,P〈0．05）。结论将量化考核绩效管理融人护理管理中,体现优劳优酬、按劳取酬的原则,是护理管理高效科学的管理模式,确保质量改进的稳定性和持续性。     

南通市2010年公共场所卫生监督的量化分级管理情况

目的分析公共场所开展卫生监督量化分级管理的效果,探索公共场所卫生监督管理的有效途径和方法。方法对南通市798家住宿场所、38家游泳场馆借鉴食品卫生量化分级管理模式进行量化分级管理。结果住宿场所、游泳场馆的实行量化分级管理后卫生管理类和卫生设施有较明显提高。结论公共场所卫生监督量化分级管理提高了卫生监管水平,对促进从业人员卫生素质的提高有着积极的作用,是一种很好的监管模式。     

Quantitative application of flow cytometry for the analysis of circulating human T cells: A preclinical pharmacokinetic study

As the application of flow cytometry to a quantitative pharmacokinetic study with adoptive T cell therapy is new, we aimed to investigate the quantitativity of flow cytometry-based analysis for the pharmacokinetic assessment of circulating human T cells in a preclinical study.We evaluated the selectivity, linearity, accuracy, precision, and sensitivity of flow cytometry-based analysis for human CD8⁺ T cells in immunodeficient mouse blood. The CD3/8/45-positive cell population was successfully distinguished from the negative population. Linear regression analysis for the calibration curve showed good linearity and recovery was approximately 100%. Acceptable inter- and intra-day precision and accuracy were observed and the lower limit of quantification (30 cells/50 μL) was validated with acceptable precision and accuracy. Blood concentrations of human CD8⁺ T cells in immunodeficient mice were then evaluated after administration using this method and the time-concentration profile of human T cells in mice was successfully assessed.The present study is the first to clarify the quantitativity of flow cytometry-based analysis for circulating human T cells in animals. The concept of the present study would be applicable to quantitative pharmacokinetics/efficacy or safety analysis of adoptive T cell therapy.     

MRI versus histological methods for time course monitoring of steatosis amount in a murine model of NAFLD

PurposeHepatic steatosis is an increasingly frequent disease with potentially severe complications. A simple quantification method is required for pretherapeutic studies to allow steatosis monitoring. This study aimed at evaluating steatosis quantification via a standard 1.5 T MRI machine in a murine model.Materials and methodsEleven groups of two rats received a choline methionine deficient diet. MRI was performed at days 0, 2, 4, 5, 6, 7 and 8, and weeks 2, 3, 4 and 5. A phased array surface coil system was used to acquire a GE T1 in- and out-of-phase multi-echo sequence, with neither cardiac nor respiratory synchronization. Steatosis was calculated with the 3-echoes method. Histological quantifications were performed both by optical analysis (percentage of fatty hepatocytes) and by automated measurement of the area of steatosis (AOS). The reference was total intrahepatic triglycerides (TIT). Protocol was approved by the ethic committee.ResultsSteatosis without inflammation, increasing with diet duration, was obtained. MRI provided better agreement (intraclass correlation coefficient) with TIT (0.889, p < 0.001) than did AOS (0.629, p = 0.001) or optical analysis (0.280, p = 0.098). MRI permitted closer monitoring of TIT over time than did AOS or optical analysis. By multivariate analysis, MRI was an independent predictor of TIT on first step and ALT on second step. A model combining these 2 variables provided excellent agreement with TIT (0.953, p < 0.001) and permitted excellent monitoring of steatosis over time.ConclusionMRI is reliable, easy, fast and superior to histological techniques for the assessment of hepatic steatosis in a murine model.     

Effects of parameters of video head impulse testing on visually enhanced vestibulo-ocular reflex and vestibulo-ocular reflex suppression

ObjectiveTo investigate the main effects of some testing and analysis variables on clinically quantified visually enhanced vestibulo-ocular reflex (VVOR) and vestibulo-ocular reflex suppression (VORS) results using video head impulse test.MethodsThis prospective observational clinical study included 19 healthy participants who underwent the VVOR and VORS tests. The effect of demographic variables, head oscillation frequency, rotation direction, visual acuity and analysis time window width and location of the recorded tests on the quantified results of both VVOR and VORS were evaluated. And specifically, for the VORS test the effect of cognitive reinforcement of the participant during testing was evaluated.ResultsA statistically significant difference was observed among the VVOR, non-reinforced VORS, and reinforced VORS tests for mean gain values of 0.91 ± 0.09, 0.6 ± 0.15, and 0.57 ± 0.16, respectively (p < 0.001). The optimized linear mixed-effect model showed a significant influence of frequency on the gain values for the reinforced and non-reinforced VORS tests (p = 0.01 and p = 0.004, respectively). Regarding the gain analysis method, statistically significant differences were found according to the short time interval sample location of the records for the initial location of the VVOR test (p < 0.006) and final location of the reinforced VORS test (p < 0.023).ConclusionSignificant differences were observed in the gain values according to VVOR and VORS testing. Head oscillation frequency is a significant factor that affects the gain values, especially in VORS testing. Moreover, in VORS testing, participant concentration has a significant effect on the test for obtaining suppression gain values. When a short time interval sample is considered for VVOR and VORS testing, intermediate time samples appear the most adequate for both tests.SignificanceThe quantified visually enhanced vestibulo-ocular reflex (VVOR) and vestibulo-ocular reflex suppression (VORS) tests have recently been added to the assortment of available clinical vestibular tests. However, despite the clinical validity of these quantified tests that appear to be of increasing clinical interest, the effects of most of the clinical testing methods and mathematical variables are not well defined.In this research we describe what are the main collecting and analysis variables that could influence to the VVOR and VORS tests. Specially for VORS test, participant concentration on test tasks will have positive effect on the measured vestibulo-ocular reflex (VOR) suppression.     

A new approach to quantitative determination of γ-glutamyl-valyl-glycine in various aquatic products

A new method for determining the content of γ-glutamyl-valyl-glycine (γ-Glu-Val-Gly, γ-EVG) using high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) with precolumn derivatization by 2,4-Dinitrochlorobenzene (CDNB) was developed. The method exhibited satisfactory linear relationship (R² = 0.9995) at concentrations ranging from 1.0 to 40.0 ng/mL. The limits of detection (LOD) and quantitation (LOQ) were 0.3 and 0.9 ng/mL, respectively. Recoveries of the target peptide in spiked samples were between 83.05% and 116.49%, showing the values of relative standard deviation (RSD) less than 10%. The intra-day and the inter-day precisions were calculated to be 7.13% and 8.54% (n = 6), respectively. The validated approach was effectively applied to quantify the γ-EVG levels (0.13–0.38 μg/g) in different aquatic products. In conclusions, a sensitive, low-cost, convenient and reliable approach was developed for the detection of γ-EVG, which can serve as a potential tool for evaluating flavor peptide quality of aquatic products.