Intraoperative Margin Assessment in Early Oral Squamous Cell Carcinoma

The surgical method of margin sampling affects local control, pathologists’ approach to margin sampling, and clarity of pathology reports. Studies have shown that exclusive reliance on tumor bed margins is associated with worse local control and should be avoided. En bloc resections and margins obtained from the resection specimen remain the “gold standard.” Successful surgical treatment of early carcinomas of the oral cavity relies on close cooperation between surgeons and pathologists on issues of specimen orientation and margin sampling.     

奥平栓联合微波治疗宫颈糜烂的临床体会

目的:探索不同类型宫颈糜烂患者适宜的治疗方法。方法:2004年10月至2008年5月我院将729例宫颈糜烂患者(单纯型140例,颗粒型235例,乳头型354例)随机分成3组,分别采用奥平栓、微波进行治疗,观察和比较其疗效和副反应。结果:奥平栓对单纯型宫颈糜烂的治疗率显著优于颗粒型和乳头型(P<0.05,P<0.01);微波对单纯型宫颈糜烂的治疗率显著优于颗粒型及乳头型(P<0.01),奥平栓加微波对颗粒型治愈率同其他两组差异无显著性意义(P>0.05),而对乳头型宫颈糜烂的治愈率在3种疗法中最高(P<0.01)。副反应以奥平栓最少。结论:单纯型及乳头型宫颈糜烂以奥平栓加微波治疗效果最佳。     

头针对小儿脑性瘫痪运动功能影响的初步研究

目的探讨头针治疗对小儿脑性瘫痪粗大运动功能的影响;为进一步开展小儿脑性瘫痪头针治疗的随机对照临床研究提供依据。方法采用头针结合康复训练治疗32例小儿脑性瘫痪患儿,并运用GMFM-66项量表对治疗前后粗大运动功能动态改变以评分法进行测评,运用PEDI量表对治疗前后功能性技巧及照顾者援助中的移动能力情况进行测评。结果经过1个疗程（3个月）治疗后,GMFM-66项分值、PEDI量表功能性技巧及照顾者援助中移动比率分值具有显著上升,与治疗前比较差异有统计学意义（P〈0.01）。结论头针治疗对小儿脑性瘫痪患儿粗大运动功能的改善产生积极的影响;临床开展头针治疗小儿脑性瘫痪大样本、多中心的随机、对照研究具有重要意义。     

Efficacy of Participation-Focused Therapy on Performance of Physical Activity Participation Goals and Habitual Physical Activity in Children With Cerebral Palsy: A Randomized Controlled Trial

Objective

To determine the efficacy of a participation-focused therapy (ParticiPAte CP) on leisure-time physical activity goal performance and satisfaction and habitual physical activity (HPA) in children with CP.

粗大运动功能测试量表与Peabody粗大运动发育量表在脑性瘫痪康复疗效评估中的应用

目的比较粗大运动功能测试量表 (GMFM )与Peabody粗大运动发育量表 (PDMS GM )两种粗大运动评估方法在脑瘫康复疗效评估中的应用价值。方法用GMFM和PDMS GM两种方法对 2 9例脑瘫儿童进行 3次评估 ,用t检验分析GMFM月相对百分比与PDMS GM月相对百分比之间的差异。结果GMFM的月相对百分比与PDMS GM的月相对百分比之间有显著性差异。结论GMFM的评估结果比PDMS GM更有利于疗效判断     

TNFα1型受体敲除对小鼠急性后肢缺血的作用

目的研究肿瘤坏死因子α1型受体(tumornecrosisfactorαreceptor1,TNFαR1)信号通路在小鼠急性后肢缺血中的作用,并探讨其作用机制。方法通过结扎TNFαR1/和野生型小鼠股动静脉及其主要分支建立后肢急性缺血模型,激光多普勒测手术前后后肢血液灌流,TUNEL染色观察细胞凋亡,凝胶电泳观察DNA凋亡梯带,免疫蛋白印迹法测定Caspase3、Bax蛋白的表达。结果术后1天TNFαR1/组缺血侧后肢血液灌流显著高于野生型组,腓肠肌TUNEL阳性细胞显著低于野生型组。TNFαR1/组缺血评分则显著低于野生型组,两组的自发截肢率分别为50%、0%。TNFαR1/组DNA凋亡梯带、Caspase3和Bax蛋白表达减弱。结论TNFαR1敲除可抑制TNFα信号通路下游死亡相关蛋白的激活,减少细胞死亡和调亡,对急性后肢缺血有保护作用。     

miR-7靶向缺氧诱导因子1α对肝癌细胞的影响*

目的： 探究微小RNA-7（miR-7）靶向缺氧诱导因子1α（HIF-1α）对肝癌细胞迁移、凋亡及干细胞特性的影响。 方法： 采用慢病毒转染肝癌细胞MGCC97H,分为对照组、miR-7 模拟物组、miR-7 干扰组（ 转染miR-7 模拟物 组+ 干扰物）、HIF-1α 过表达组（ 转染pc-HIF1α）和共转染组（ 转染miR-7 模拟物+pc-HIF1α）,RT-PCR 检测各 组细胞miR-7、HIF-1α mRNA 表达量,免疫印迹检测各组HIF-1α 蛋白的表达量,Transwell 实验检测细胞迁移能 力,流式细胞法检测细胞凋亡情况,软琼脂克隆形成实验分析其干细胞特性,荧光素酶实验验证miR-7 与HIF-1α 靶向关系。结果： miR-7 模拟物组细胞HIF-1α 蛋白表达、迁移率、克隆形成率低于对照组,凋亡率高于对照组; HIF-1α 过表达组细胞迁移率、克隆形成率高于对照组,凋亡率低于对照组;共转染组HIF-1α 蛋白表达、细胞迁 移率、克隆形成率低于HIF-1α 过表达组,凋亡率高于HIF-1α 过表达组。结论： miR-7 靶向HIF-1α 可降低肝癌细 胞迁移能力及干细胞特性,促进细胞凋亡,miR-7 及HIF-1α 可能是肝癌的潜在治疗靶点。     

Fibroblast expression of α-smooth muscle actin, α2β1 integrin and αvβ3 integrin: influence of surface rigidity

Open wound contraction necessitates cell and connective tissue interactions, that produce tension. Investigating fibroblast responses to tension utilizes collagen coated polyacrylamide gels with differences in stiffness. Human foreskin fibroblasts were plated on native type I collagen-coated polyacrylamide gel cover slips with different rigidities, which were controlled by bis-acrylamide concentrations. Changes in alpha smooth muscle actin (αSMA), α2β1 integrin (CD49B) and αvβ3 integrin (CD-51) were documented by immuno-histology and Western blot analysis. Cells plated on rigid gels were longer, and expressed αvβ3 integrin and αSMA within cytoplasmic stress fibers. In contrast, cells on flexible gels were shorter, expressed α2β1 integrin and had fine cytoskeletal microfilaments without αSMA. Increased tension changed the actin makeup of the cytoskeleton and the integrin expressed on the cell's surface. These in vitro findings are in agreement with the tension buildup as an open wound closes by wound contraction. It supports the notion that cells under minimal tension in early granulation tissue express α2β1 integrin, required for organizing fine collagen fibrils into thick collagen fibers. Thicker fibers create a rigid matrix, generating more tension. With increased tension cytoskeletal stress fibers develop that contain αSMA and αvβ3 integrin that replaces α2β1 integrin, consistent with cell switching from collagen to non-collagen proteins interactions.     

急性放射性口腔黏膜反应防治进展

急性放射性口腔黏膜反应是头颈部恶性肿瘤在放射治疗过程中最常见的副反应,射线除直接损伤黏膜外,还造成照射野内的毛细血管发生反应性扩张、充血,出现红斑,进而形成黏膜糜烂、溃疡,给病人造成身心痛苦,轻者疼痛,影响吞咽、进食及语言等,重者影响到放射治疗的终止或暂停。因此,寻求安全有效的药物防治放射性口腔黏膜反应已日益受到重视。现就其发生机制、防治的研究进展综述如下。