Electrochemical study of a metallothionein modified gold disk electrode and its action on Hg2+ cations

A novel protein monolayer modified electrode has been prepared by the self-assembly of metallothionein (MT) at a gold disk electrode. The properties of MT in Tris–HCl buffer and in the monolayer are studied by using cyclic voltammetry and differential pulse voltammetry with a gold disk electrode. In the negative sweep, the voltammogram of MT in buffer shows two small peaks and different electrochemical behaviour from that at a mercury electrode. Cd²⁺ complexed to the thionein can easily be replaced by Hg²⁺ ions, and Hg²⁺ ions can firmly adsorb in the MT monolayer with a saturation coverage of (2.78±0.29)×10^?10 mol cm^?2. This behaviour has been used to preconcentrate trace Hg²⁺ for its determination by cathodic stripping differential pulse voltammetry. The cathodic stripping peak current is proportional to Hg²⁺ concentration in the range of 0.15–3 μM and the detection limit is ca. 0.08 μM (16 ppb) with a 2 min open circuit accumulation step. The relative standard deviation is 7.2% at 0.4 μM Hg²⁺ concentration (n=4). At higher concentration the adsorption of Hg²⁺exhibits a response similar to that expected for a Langmuir adsorption isotherm with the stability constant of (4.0±0.2)×10⁵ M^?1.     

Synthesis and characterization of a catechol-terminated alkanethiolate monolayer adsorbed on gold

Structural, redox, and metal ion binding characterizations of the monolayer formed by the chemisorption of 3,4-dihydroxyphenethyl mercaptan (DHPM) on gold are described. This system was explored as a model for investigations of surface-immobilized molecules that contain redox-transformable coordination sites for binding metal ions from solution. The characterizations were carried out using infrared reflection spectroscopy, X-ray photoelectron spectroscopy, cyclic voltammetry, and long optical pathlength thin-layer spectroscopy. The XPS data show that this monolayer adsorbs as a thiolate, and the IRS results indicate that the average orientation of the aromatic ring plane is close to the surface normal. Cyclic voltammetry reveals that the pendant catechol/quinone undergoes the expected two-electron, two-proton redox transformation; however, the oxidized form of the couple is unstable, particularly in alkaline aqueous solutions. A determination of Cu(II) binding ability to the surface-immobilized catechol moieties, using a long optical pathlength thin-layer cell to follow the depletion of Cu(II) from solution, indicated that the complexation of the metal was not substantially different from that for the solution form of catechol. The instability of the oxidized form of the ligand, nevertheless, precluded an assessment of its metal ion binding ability. An approach using these data for estimating the conditional formation constant for the surface complexation reaction is also developed.     

三金片联合抗生素治疗尿路感染临床分析

目的:探讨三金片联合头孢克肟治疗尿路感染的临床疗效。方法:选取200例尿路感染患者,将其随机分为治疗组(三金片联合头孢克肟)和对照组(头孢克肟),比较分析两组患者的临床疗效。结果:治疗组尿急、尿频症状消失时间和尿常规检查白细胞、红细胞好转时间较对照组明显减少,治疗组总有效率明显高于对照组(P<0.05)。结论:三金片联合头孢克肟治疗尿路感染有较好疗效,并能有效改善患者的免疫功能。     

山西王家岭煤矿3．28透水事故中获救的115例矿工身体状态初步评估与分析

目的通过对受困189h的115例获救矿工的身体情况评估并结合当时环境进行分析，为后期救治提供参考。方法对进入病房的115例获救矿工即刻测量生命体征、评估神志状态；即刻化验血常规、血电解质和磷、血气分析、血糖，进行心电图检查。结果获救矿工共115例，均为男性，平均年龄（39±3）岁，入院时体温（36±0．2）℃，均神志清楚；筛查发现7例血糖低于正常值；8例轻度低钾、低钠，脱水征象不明显；血磷检测发现在入院时有3例轻度下降，治疗中出现1例轻度下降；血白蛋白检查啦陉度低玎正：常值，无明显恶液质表现；心血管系统检查有4例血压偏低70～90／50～60mmHg，没有全身组织灌注不足表现：有6例心率低于60次／分，均为窦性心率；有2例肌钙蛋白一过性升高，2例心电图见广泛sT段改变；30％病例化验结果提示尿素氮轻度升高，没有肌酐升高病例；血气分析检查pH值均在正常范围，没有明确代谢性酸中毒发生，没有低氧血症存在，少部分病例有二氧化碳分压轻度下降；1／3患者尿中存在酮体＋-＋＋＋；部分患者出现轻度转氨酶升高和总胆红素升高。床旁B超检查未发现形态学改变；未发现下肢静脉血栓形成患者。结论部分矿工存在不同程度的多系统异常，特别是电解质、血糖和心血管系统。     

Criterion-related validity and test–retest reliability of the 20 m Square Shuttle Test

We assessed validity and reliability of the new 20m square shuttle run test (SST) for predicting maximal oxygen uptake (V O(2max)) and compared it with its predecessor, the 20m Multistage Shuttle Run Test (MST). In a repeated-measures randomised-block design, 74 healthy adult males performed the SST, the MST and a treadmill test (TT). To assess reliability, 40 of the total 74 volunteers were randomly-selected to perform the SST and MST twice. Unlike the SST (p>0.05), mean predicted V O(2max)(V predO(2max)) from the MST was significantly increased from that measured during the TT (p<0.05). The V predO(2max) from SST and MST correlated with TT V O(2max) at r=0.95 (p<0.001) and r=0.63 (p<0.001), respectively. Prediction error of SST was -0.3+/-3.3mlkg(-1)min(-1) with a coefficient of variation of +/-3.5%, while the equivalent values for MST were 4.2+/-7.3mlkg(-1)min(-1) and +/-7.4%. Mean test-retest V predO(2max) did not differ for both SST and MST (p>0.05), while the corresponding test-retest correlation coefficients were r=0.85 (p<0.001) and r=0.72 (p<0.001). Reliability errors in 95% limits of agreement were 0.3+/-4.8 and 0.6+/-6.8mlkg(-1)min(-1) while coefficients of variation were +/-5.2% and +/-6.8% for the SST and MST, respectively. It is concluded that SST is a more valid proxy than MST for predicting laboratory V O(2max) based on the current procedures, while both tests are sufficiently reliable in healthy male adults.     

The Detectability and Localization Accuracy of Implanted Fiducial Markers Determined on In-Room Computerized Tomography (CT) and Electronic Portal Images (EPI)

Many different methods of image guidance are available for radiotherapy treatment (IGRT). The aims of the study were (1) to determine the optimal diameter of gold markers for IGRT to the prostate; (2) to compare, using the Siemens Primatom, the relative merits of in-room computerized tomography (CT) and electronic portal image (EPI) for locating the marker seeds. Gold markers of differing widths were embedded in 2 phantoms (perspex slabs and anthropomorphic). Images were acquired with an amorphous silicon flat panel detector (Siemens Optivue 500) and with the in-room CT scanner (Siemens Somatom Balance). The EPIs were reviewed independently by 6 operators to determine which diameter marker could be best visualized. The optimal marker technique was determined by comparing the investigators' observed marker co-ordinates with the known locations within the phantom. The visibility of all markers on anterior-posterior EPIs was 100%. On the lateral EPI, of a possible 180 visualizations of 1.2-, 1.0-, and 0.8-mm diameter markers, 176 (97.8%), 151 (83.9%), and 132 (73.3%), respectively, were successful. On EPI, the average deviation of fiducial markers from the known position was less than 0.5 mm in any direction. On CT, the largest deviation (2.17 mm) of markers from the known coordinate position was in the superior-inferior direction, reflecting the 3.0-mm slice thickness used. EPI accurately located internal markers in all dimensions. The availability of “gold standard” CT imagery at the treatment unit does not improve how accurately the position of markers in a phantom can be defined compared with EPI. However, CT imagery does provide important soft tissue information, the benefits of which are being investigated further.     

反坦克地雷对坦克内绵羊的致伤效应

为了解遭受反坦克地雷袭击时坦克内人员的致伤情况，采用3枚反坦克破甲地雷置于坦克覆带下，坦克内布放绵羊，用电雷管引爆地雷，检查动物伤情。结果地雷被弹爆后，将车轮炸飞，履带炸断，坦克内12只绵羊有12耳出现轻度损伤，并伴有内脏轻度损伤。提示反坦克地雷袭击坦克后能造成动物听器官轻度损伤，强烈震动也可造成动物的损伤。     

煤矿井下矿工跟骨定量超声及骨代谢的改变

目的:探讨煤矿井下矿工骨代谢变化。方法:采用UBIS3000型定量超声仪,对140名井下矿工跟骨进行定量超声测定,并用化学发光免疫法测定尿脱氧吡啶酚(DPD)排泄浓度。用同样的方法和内容对井上工作人员跟骨进行定量超声测定和用化学发光免疫法测定尿DPD的排泄浓度。结果:井下矿工跟骨超声振幅衰减(BUA)、超声声速(SOS)和刚度(STI)各年龄段组与井上组比较均显著降低(P<0.01)。尿DPD排泄浓度井下20～30岁和31～40岁各年龄段组与井上组比较均显著增高(P<0.01)。结论:井下作业人员,因长年受缺少日光、缺氧、空气中有毒气体等因素的影响,可导致骨代谢异常,骨吸收增加。     

金双歧联合蒙脱石散治疗小儿病毒性肠炎的疗效

〔摘 要〕 目的：探讨金双歧联合蒙脱石散治疗小儿病毒性肠炎的效果。 方法：选取厦门大学附属第一医院杏林分院 2019 年 10 月至 2020 年 9 月收治的病毒性肠炎患儿 79 例,根据治疗方案不同分为观察组（41 例）和对照组（38 例）。 对照组予以蒙脱石散治疗,观察组予以金双歧联合蒙脱石散治疗,比较两组患儿疗效、不良反应情况、治疗前后炎症 因子水平和心肌损害情况。 结果： 观察组患儿治疗总有效率为 97.56 %,高于对照组的 81.58 %,差异具有统计学意义 （P ＜ 0.05）。治疗后,两组患儿血清肿瘤坏死因子 –α（TNF–α）、白细胞介素 –6（IL–6）、IL–10 水平均较治疗前明显降低, 且观察组低于对照组,差异具有统计学意义（P ＜ 0.05）。治疗后,两组患儿血清肌酸激酶（CK）、肌酸激酶同工酶 MB （CK–MB）、心肌肌钙蛋白 I（cTnI）水平均较治疗前明显降低,且观察组低于对照组,差异具有统计学意义（P ＜ 0.05）。 两组患儿治疗过程中均未见不良反应。 结论：金双歧联合蒙脱石散治疗小儿病毒性肠炎能显著缓解炎症状态,减轻心肌 损害,效果显著,安全性高。     

In vivo tumor-targeted dual-modal fluorescence/CT imaging using a nanoprobe co-loaded with an aggregation-induced emission dye and gold nanoparticles

As an intensely studied computed tomography (CT) contrast agent, gold nanoparticle has been suggested to be combined with fluorescence imaging modality to offset the low sensitivity of CT. However, the strong quenching of gold nanoparticle on fluorescent dyes requires complicated design and shielding to overcome. Herein, we report a unique nanoprobe (M-NPAPF-Au) co-loading an aggregation-induced emission (AIE) red dye and gold nanoparticles into DSPE-PEG₂₀₀₀ micelles for dual-modal fluorescence/CT imaging. The nanoprobe was prepared based on a facile method of “one-pot ultrasonic emulsification”. Surprisingly, in the micelles system, fluorescence dye (NPAPF) efficiently overcame the strong fluorescence quenching of shielding-free gold nanoparticles and retained the crucial AIE feature. In vivo studies demonstrated the nanoprobe had superior tumor-targeting ability, excellent fluorescence and CT imaging effects. The totality of present studies clearly indicates the significant potential application of M-NPAPF-Au as a dual-modal non-invasive fluorescence/X-ray CT nanoprobe for in vivo tumor-targeted imaging and diagnosis.