口内切口联合耳后真皮-脂肪游离移植治疗微小唇裂

目的 探讨一种修复微小唇裂的方法.方法 在患侧人中嵴对应的黏膜侧做口内切口,将口轮匝肌解剖复位;在患侧上唇通过皮下隧道游离移植,从耳后切取的真皮一脂肪形成人中嵴;红、白唇交界处用Z成形术等方法修复,红唇凹陷用耳后真皮-脂肪游离移植充填;患侧鼻畸形采用悬吊固定等方法矫治.结果 共治疗8例,7例获随访,最长18个月,均取得满意效果.结论 口内切口联合耳后真皮一脂肪游离移植治疗微小唇裂是一种理想方法.     

烧伤早期切痂脱细胞异体真皮基质与自体皮浆复合移植的临床应用

目的探讨脱细胞异体真皮基质与自体皮浆复合移植的可行性。方法在8例患者Ⅲ。烧伤创面早期切痂,13个部位采用18块脱细胞真皮基质移植固定,在异体皮的真皮面上均匀涂布备好的皮浆,覆盖于脱细胞真皮基质上,缝合包扎固定。同一肢体其余创面常规用自体皮浆加异体皮移植做对照观察,皮浆与创面比例为1：5～1：8。结果18块脱细胞异体真皮基质复合移植皮肤成活率94％,随访3～13个月皮肤弹性及色泽好、瘢痕轻、耐磨,愈合质量优于对照部位创面。术后3个月组织切片表皮真皮连接紧密,可见钉突样结构,胶原纤维排列较规整;单纯皮浆移植皮肤为瘢痕皮肤结构。结论应用脱细胞真皮基质与自体皮浆复合移植,明显优于单纯的皮浆移植,具有较好的临床应用前景。     

大鼠真皮间充质干细胞的体外长期培养及胶原海绵对其生长的影响

从新生大鼠真皮细胞中分离多能干细胞，体外长期传代培养，观察细胞形态和超微结构的变化。检测细胞增殖和分化能力的改变及胶原基质对细胞生长的影响。揭示体外长期传代培养对大鼠真皮间充质干细胞生物学性状的影响，结果表明；大鼠真皮组织中的间充质干细胞体外培养至180d，传至25代的细胞仍具有干细胞的特征；形态的均一，细胞核结构原始，细胞器不发达；体外增殖迅速，保持了向成骨细胞和软骨细胞分化的潜能，胶原基质成分可促进细胞的生长，而胶原海绵支架更利于细胞的三维生长。结论是体外长期传代培养后大鼠真皮间充质干细胞仍保持良好的干细胞特性，为真皮间充质干细胞的深入研究与应用提供了实验依据。     

Isolated primary osteoma cutis of the head: Case report

Osteoma cutis is a rare disease in which there is formation of bony tissue in the skin that causes deformities. The etiology remains unknown and its treatment is controversial. A rare case of primary osteoma cutis in the face and scalp, which was not associated with any syndrome, is described. The patient was treated with surgery and topical retinoic acid. The retinoic acid treatment resulted in an improvement of the frontal area, and stabilized the disease over a two-year follow-up period. Surgical resection was a simple treatment with a quick recovery, minimal scarring and no local recurrence. The patient was followed for two years and presented a satisfactory result.The treatment of osteoma cutis is quite variable, and surgery is the most frequently reported treatment. However, a combination of clinical and surgical treatments seems to be an efficient way to manage these patients.     

真皮来源成体多能干细胞的分离鉴定和生物学性状的研究

目的:从新生大鼠和成人真皮组织分离鉴定多能干细胞,并研究其生物学性状。方法:以新生大鼠背部皮肤和成人包皮为研究对象,依据体外贴壁生长的性状,采用酶消化法分离真皮多能干细胞,通过定向诱导其多向分化实验进行鉴定。结果:早期贴壁筛选的新生大鼠和成人皮肤组织原代真皮细胞克隆经过传代培养后细胞形态均一,约90%的细胞处于 Go/G1期,细胞表面波形蛋白表达阳性,但Ⅷ因子和角蛋白表达阴性。经诱导分化后可向成骨细胞和脂肪细胞分化,具有多能分化潜能。体外培养的多能干细胞具有异质性,含有体积大小不同的细胞亚群。绿色荧光蛋白 GFP 基因转染真皮多能干细胞可获得良好表达。结论:大鼠和人真皮组织中均存在成体多能干细胞,提示真皮可能是成体多能干细胞的又一来源。     

真皮多能干细胞促进大鼠急性放射损伤造血恢复的研究

目的 观察真皮多能干细胞移植对大鼠急性放射损伤后造血恢复的作用。方法 分离新生大鼠真皮多能干细胞体外培养至第10代用于研究，观察干细胞层体外对正常大鼠骨髓粒-巨噬系祖细胞和红系祖细胞集落生长的影响；进一步将真皮多能干细胞经尾静脉输入5Gyγ射线全身照射的大鼠体内，检测移植后骨髓有核细胞、粒-巨噬系祖细胞集落CFU—GM、红系祖细胞集落CFU—E和外周血白细胞数量、血红蛋白含量等指标的恢复情况，观察4周后动物的存活率。结果 真皮多能干细胞层体外可促进正常大鼠CFU—GM和CFU—E集落的生长；全身输入干细胞促进了5Gyγ射线全身照射动物的外周血白细胞和骨髓有核细胞、CFU-GM和CFU—E集落数的恢复。实验组动物4周后的存活率也明显高于对照组。结论 真皮多能干细胞具有一定支持造血的功能，可促进急性放射损伤大鼠的造血恢复，为造血损伤相关疾病的干细胞治疗提供新的细胞来源途径。     

主动脉平滑肌细胞在三维培养中产生弹性纤维的研究

目的 研究主动脉平滑肌细胞(ASMC)在仿真皮替代物的三维培养中是否能产生弹性纤维。方法 取1周龄SD大鼠胸主动脉，进行原代和传代培养，以α肌动蛋白免疫细胞化学染色鉴定平滑肌细胞。然后，将ASMC与胶原、甲壳素及糖胺多糖组合形成的凝胶混合，进行三维培养；培养1周和2周后，用Gomori醛复红染色和弹性蛋白免疫细胞化学染色，检测ASMC胶原凝胶。结果传代细胞经α肌动蛋白免疫细胞化学染色鉴定，98％以上为ASMC。培养1周和2周的ASMC胶原凝胶中，均显示有弹性纤维存在。结论 ASMC在仿真皮替代物的三维培养中能够产生弹性蛋白，并形成弹性纤维。     

A study of reactions to Corynebacterium Parvum (C. parvum) in the brain and dermis of the rat

Summary A comparative analysis was made of the histological changes caused by Corynebacterium Parvum (C. parvum) injected into the brain and dermis of normal and C. parvum presensitized rats. Control rats were injected with saline. It was shown that for approximately 3 days after the intracerebral injection the brain reacted with a polymorphonuclear cell infiltration. This reaction was replaced in order by lymphocytes, macrophages, and finally by epithelioid cells progressing to granuloma. The histological changes were similar but more intense in the dermis than in the brain. Lymphocytes reached a peak by day 7, macrophages by day 3, and epithelioid cells by day 18. In comparison to the dermis, the cellular infiltration in the brain is more delayed, less intense and of longer duration. Intracerebral saline induced a moderate lymphocytic infiltrate, and in C. parvum presensitized rats a more intense macrophagic response. It is concluded that the brain of the rat, especially in the presensitized animal, mounts an inflammatory response to C. parvum which is morphologically similar to that seen in the skin.Supported in part by The Ludwig W. Fröhlich Brain Tumor Research Fund     

复方壳多糖真皮替代物体外抗菌作用的研究

目的 研究复方壳多糖真皮替代物对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白念珠菌的体外抑菌作用。方法 制备复方壳多糖真皮替代物和复方胶原凝胶真皮,分别为实验组和对照组,各取50μL滴加在涂满细菌或真菌的平板,每种菌实验组和对照组样本各6个,孵育24h后观察抑菌环。结果 复方壳多糖真皮替代物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌具有抑菌作用,抑菌环均大于复方胶原凝胶真皮替代物,差异有统计学意义。两组对白念珠菌无抑菌作用。结论 复方壳多糖真皮替代物是有体外抗细菌作用的组织工程产品。