In-vitro synthesis of steroid hormones in corpora lutea from normal women and women treated with 300 micrograms norethisterone.

The in-vitro oestradiol (E2) and progesterone (P) production by corpora lutea (CL) obtained at sterilization from 30 untreated women and 43 women treated with norethisterone (NET) 300 micrograms daily was measured. The CL were obtained at different stages of the luteal phase in the untreated women [luteinizing hormone (LH) 0 to +3, n = 7; LH +4 to +7, n = 7; LH +8 to +11, n = 9; LH +12 to menses, n = 7] and on days LH +8 to +11 or cycle days 22 to 26 in the NET-treated women. In the treated women, four types of ovarian reaction were identified. Four women showed ovarian reaction Type A (completely inhibited ovarian activity), 14 women Type B (marked follicular activity, but no luteal function), 12 women Type C (normal follicular activity, followed by insufficient luteal function) and 13 women Type D (apparently normal follicular and luteal activity). The CL were incubated in Eagle's medium with and without stimulation by human chorionic gonadotrophin (HCG) for 2 and 4 h. In the untreated women, P and E2 production increased significantly with both incubation time and stimulation by HCG throughout the luteal phase, except in the late luteal phase (LH +12 to menses) where P increased (P less than 0.01) only after 4 h stimulation by HCG. The maximal production of P was found after 4 h incubation with HCG stimulation of CL tissue in the early-mid luteal phase (LH +4 to +7).(ABSTRACT TRUNCATED AT 250 WORDS)     

羊膜负载骨髓间充质干细胞与表皮细胞对放创性皮肤损伤促愈合研究

目的探讨治疗放创性全厚皮肤缺损创面的方法及效果. 方法贵州小香猪8只,每只背部脊柱两侧均有放创性全层皮肤缺损圆形创面(Ф3.67cm)各3个,共48个创面.将经处理的人羊膜(human amniotic mambrane, HAM)分别负载自体骨髓间充质干细胞(mesenchymal stem cells, MSCs)和表皮细胞,移植到其左侧24个创面作为实验组(A组);以单纯无种植细胞的HAM敷盖其右侧前16个创面(B组);以单纯油纱布敷盖其右侧后8个创面(C组).B、C作为对照组.观察移植后1～3周内各组创面愈合、肉芽组织生长及上皮化等情况,并进行创面组织HE染色及vWF免疫组织化学检测.用图像分析法测算各组各时间点创面平均面积(cm2),并计算其愈合百分率. 结果 C组于伤后 22～23天愈合,B组于伤后19～21天愈合;A组于伤后15～17天愈合,较B、C组分别提前6～7天和5～6天,愈合质量好.移植15～17天,A组与B、C组创面平均残留面积及愈合面积百分率比较,差异有统计学意义(P＜0.01). A组创面的新生上皮已完全覆盖整个创面,肉芽组织生长旺盛,肉芽组织中vWF、成纤维细胞和毛细血管含量丰富,可见胶原沉积;B、C组创面仍见许多炎性细胞浸润,肉芽组织中vWF、成纤维细胞和毛细血管含量少,胶原沉积不明显. 结论 HAM负载自体MSCs和表皮细胞植入对放创性全厚皮肤缺损创面有较好的促愈合作用,愈合质量较高.     

放疗中呼吸引起的组织深度波动对吸收剂量的影响

目的研究放疗中呼吸引起的组织深度波动对吸收剂量的影响。方法在不同能量、组织深度波动幅度、呼吸频率及平均测量深度时，分别用研制的能模拟组织深度波动的辐射剂量测量水箱进行测量，以获得“组织深度波动因子”（Fdw）进行比较。结果组织深度波动确实对实际吸收剂量有影响，而且组织深度波动幅度、呼吸频率及射线能量越大，Fdw值越小，即对吸收剂量的影响越大，而Fdw与平均测量深度无关。结论放疗中必须根据患者的呼吸频率、所用射线类型及呼吸引起的组织深度波动幅度用Fdw对肿瘤或组织的实际吸收剂量进行修正，以提高疗效。     

High-dose contrast-enhanced computed tomography (CECT) with iopamidol in the detection of cerebral metastases

Summary Intravenously admistered iodinated contrast media have been demostrated, since early experience with computed tomography of the brain, to improve clinical value of the procedure for detecting intracranial lesions. There is no universal agreement about the amount and the method of administration of the contrast medium. Many authors maintain that the use of large doses gives better results for the diagnosis of tumors and metastases. The purpose of this paper is to evaluate the tolerance of iopamidol administered by rapid intravenous infusion in a large number of patients undergoing contrast enhanced computed tomography to detect brain metastases. The authors examined 969 consecutive adult patients suffering from lung cancer, brain metastases have been detected in 17% of cases. Adverse reactions to contrast media occurred in 3 patients. Non ionic contrast media are recommended in this diagnostic procedure.     

新疆核试验场周围土壤中90Sr137Cs水平

用放射化学分析方法,对新疆核试验场周围10个调查区和远离核试验场位于上风向的5个对照区采取的土壤样品进行了⁹⁰Sr、¹³⁷Cs分析测定。核试验场周围调查区土壤中⁹⁰Sr累积平均沉降量为1.5×10³Bqm^-2,¹³⁷Cs为4.9×10³Bqm^-2;对照区⁹⁰Sr累积平均沉降量为1.3×10³Bqm^-2,¹³⁷Cs为3.9×10³Bqm^-2。调查区和对照区表层土壤中州⁹⁰Sr,¹³⁷Cs放射性平均水平无显著性差异。通过估算得出了调查区⁹⁰Sr、¹³⁷Cs对居民产生约定有效剂量当量分别为78.3μSv(集体剂量当量负担为23.9人·sv)和270μSv(集体剂量当量负担为82.3人·sv),对照区分别为70.5μSv,(集体剂量当量负担为14.1人·sv)和220μSv(集体剂量当量负担为14.1人·sv)。两个地区居民受到来自核试验产生的⁹⁰Sr和¹³⁷Cs给予人体的内照射剂量当量负担基本上是一样的。可认为⁹⁰Sr、¹³⁷Cs对新疆核试验场周围没有造成严重的局部污染。对居民的身体健康不致于产生不良影响。     

Appropriate Replacement Dose of Thyroxine in Primary Hypothyroidism

ABSTRACT An ultrasensitive thyrotropin (TSH) assay was used to determine how many of 65 patients with primary hypothyroidism on thyroxine (T₄) replacement therapy had suppressed serum TSH. In 13 patients (20%) TSH levels ≤0.1 mlU/l were found, indicating an overdose of thyroxine. After correction of the dose, 48 patients had normal TSH values. Their mean dose of thyroxine was 119 μg/24 hours, and the appropriate replacement dose tended to decline with advancing age. The serum level of thyroid hormones during replacement therapy with thyroxine very imperfectly reflected serum TSH values. It is concluded that overdose of thyroxine is common when suppressed serum TSH is used as an end point. Biochemical follow-up of replacement therapy with thyroxine in primary hypothyroidism therefore requires the use of an ultrasensitive TSH assay in order to detect such suppression. Serum levels of thyroxine or triiodothyronine (T₃) during thyroxine therapy are poor indicators of pituitary TSH secretion and are therefore not useful as parameters of adequate thyroxine dosage.     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

淋巴细胞亚群微核表达的辐射效应研究

简述了淋巴细胞亚群微核检测方法、淋巴细胞亚群自发微核率和影响因素，以及关于电脑射效应的研究现状，为微核法用于辐射生物剂的未来发展提供了有益信息。     

安氟醚吸入与预注爱肌松对其肌松时效的影响

单次静注爱肌松（０．３ｍｇ·ｋｇ－１，对照组）于３．１７±０．８１分钟（ｘ±ｓ）可获得满意的气管插管条件。预注组（１０例）首次量０．２７ｍｇ·ｋｇ－１于预注剂量（０．０３ｍｇ·ｋｇ－１）后５分钟给予，可使爱肌松的起效时间缩短至０．９３±０．１７分钟（Ｐ＜０．００１），ＴＯＦ无反应期、２５％和９０％恢复时间两组无显著差别（Ｐ＞０．２５）。全部病例均获得了良好的气管插管条件，预注的优点还在于能评估患者对所选用非去极化肌松药的敏感性。预往后ＴＯＦ肌电图Ｔ１抑制＞１０％较无明显ＴＯＦ抑制者肌松恢复时间显著延长（Ｐ＜０．０５）。同时发现吸入安氟醚可使爱肌松作用时效延长。     

麦胚提取物对辐射损伤修复的实验研究

目的 研究麦胚提取物对小鼠辐射损伤修复的调节和保护作用。方法 采用辐射前或辐射同时饲喂一定量的麦胚提取物，观察小鼠经X射线一次性全身照射后的临床症状、30d存活率、骨髓微核率、外周血白细胞总数在不同时间的变化。结果 与对照组(单纯照射)比较，饲喂麦胚提取物可使小鼠的头面部皮肤、小肠黏膜、肾脏损伤症状得到明显改善；30d存活率为86．17％(P＜0．01)，提高存活率41．79％，保护系数为1．72；骨髓微核率4．62‰；比对照组(12．14‰)降低(P＜0．01)；外周血白细胞总数在7，13，20，30d均显回升(P＜0.05，P＜0．01，P＜0．01，P＜0．01)。结论 麦胚提取物对小鼠辐射损伤修复有一定程度的调节和保护作用，对于辅助肿瘤放射治疗具有重要意义。