巨细胞病毒IgG特异性抗体亲和指数测定方法的研究

目的：建立一种快速、简便的检测巨细胞病毒IgG抗体（CMV-IgG）亲和指数（AI）的方法,区分CMV原发与非原发感染。方法：对检测方法的各个环节进行了比较研究,确定最佳检测方法并对218份标本（187例既往感染,13例继发或再感染,10例可疑原发感染和8例原发感染）进行检测,比较了两种结果表示方法。结果：当用U/ml和OD值表示结果时,其敏感性、特异性和方法可用度分别为75%、91.4%、67.2%、62.5%、95.7%、61.7%。结论：用35mM二乙胺简便的洗脱法,以标准曲线定量标本中CMV-IgG含量并计算AI的方法适用于CMV-IgGAI的测定。     

Prime–boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI

The efficacy of different vaccination schedules was evaluated in 17-day-old Pekin ducks using an experimental inactivated whole virus vaccine based on the H5N9 A/chicken/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing a synthetic HA gene from an Asian H5N1 isolate (A/chicken/Indonesia/7/2003). Full protection against clinical signs and shedding was induced by the different vaccination schemes. However, the broadest antibody response and the lowest antibody increase after challenge were observed in the group of ducks whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, suggesting that this prime-boost strategy induced optimal immunity against H5N1 and minimal viral replication after challenge in ducks. In addition, this prime-boost vaccination scheme was shown to be immunogenic in 1-day-old ducklings.     

Effect of HDL and apo AI on PGE_2 Production by Monocyte-Derived Macrophages

Today,coronary artery disease( CAD) is oneof leading causes of death not only in the Westernworld,but also in Asian area including China[1,2 ] .Many studies showed that both high densitylipoprotein ( HDL) and its major protein compo-nent apolipoprotein AI( apo AI) are factors thathelp prevent CAD.It is widely believed their pri-mary function is to remove excess cholesterol fromextrahepatic tissue to liver for catabolism,a pro-cess referred to as reverse cholesterol transport,RCT[3 ] . …     

泸州地区正常人群载脂蛋白A_I,B_(100)及脂蛋白(a)的调查

测定泸州地区250名健康人血清中的载脂蛋白（Apo）A－I．B_100及脂蛋白（a）［LP（a）］水平。结果:ApoA－I：1．29±0．24g／L，ApoB_100:0．66±0．18g／L，Apo－I／ApoB_100：2．05±0.64,LP（a）：上限值为300mg／L，并与几个城市的结果作了比较。     

替米沙坦对老年高血压不稳定性心绞痛患者脂联素及C反应蛋白的影响

目的探讨过氧化物酶体增殖物活化受体γ(PPARγ)激动剂替米沙坦对伴有高血压的老年不稳定性心绞痛患者血清脂联素及C反应蛋白的影响。方法用随机方法(分层抽样法)将120例伴有高血压的老年不稳定性心绞痛患者分为替米沙坦组(60例)和培垛普利组(60例),分别用酶联免疫吸附法及酶标多克隆抗体夹心法测定患者在治疗前和治疗后6个月的血清脂联素、高敏C反应蛋白(hs-CRP)浓度,并观察血糖、胰岛素敏感性指数(ISI)及血脂水平的改变。结果用培垛普利及替米沙坦治疗6个月后,平均血压显著下降〔(126±16)至(104±11)mmHg及(125±14)至(103±12)mmHg,均为P<0.05〕,血清脂联素浓度及ISI明显升高〔(5.8±2.7)至(6.3±2.5)mg/L,(5.9±2.9)至(8.4±3.1)mg/L及0.0075±0.0015至0.0088±0.0019,0.0069±0.0013至0.0137±0.0037,P<0.05及P<0.01〕,而hs-CRP明显下降〔(0.79±0.13)至(0.54±0.09)mg/L及(0.80±0.12)至(0.38±0.06)mg/L,P<0.05及P<0.01)〕,尤以替米沙坦组较甚。替米沙坦组治疗6个月后,心血管事件发生率显著下降(P<0.01)。结论与培垛普利相比,替米沙坦能够明显升高伴有高血压的老年不稳定性心绞痛患者血清脂联素浓度及显著降低其血浆hs-CRP水平和心血管事件发生率。     

Mechanisms of orofacial sensory processing in the rat insular cortex

Background

Orofacial structures involve multifarious organs, including the lips, tongue, soft and hard palates, teeth, periodontal ligaments, salivary glands, and facial skin. These organs process multiple types of sensory information that contributes to performance of the finely regulated orofacial movements. Among the types of sensory information, gustation is processed in the insular cortex, and, thus, part of the insular cortex is called the gustatory cortex. Moreover, the insular cortex receives not only gustatory information but also information on other somatosensory and chemical sensations, including nociception, thermoreception, audition, and olfaction.

六神丸对食管癌移植瘤细胞凋亡指数及B淋巴细胞瘤-2基因的影响

目的探讨六神丸对食管癌移植瘤组织细胞凋亡与B淋巴细胞瘤-2基因(Bcl-2)表达的影响及相关机制。方法通过皮下注射细胞悬液建立食管癌移植瘤模型,将48只裸鼠随机分为空白组、模型组、顺铂组(DDP)、六神丸高剂量组、六神丸中剂量组、六神丸低剂量组,按照给药方案用药,用药20 d后处死动物,剥取瘤组织,TUNEL(原位末端标记法)检测瘤体组织细胞凋亡指数,免疫组化法检测瘤体组织Bcl-2的表达。结果六神丸高、中、低剂量各组细胞凋亡指数均高于模型组,给药各组瘤体组织内Bcl-2的表达活性均明显下降。结论六神丸通过下调肿瘤组织Bcl-2表达活性、促进细胞凋亡是六神丸抑瘤作用机制之一。