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91.
92.
目的:建立一种快速、有效的血清铜蓝蛋白(CP)筛查方法。方法:醋酸纤维膜电泳分离血清CP,盐酸联苯胺显示法。结果:用此方法可明确检测出Wilson氏病患者血清中CP含量的变化。结论:醋酸纤维膜电泳检测血清CP是一种有效的血清CP筛查方法。 相似文献
93.
R. Häusler A. Burger W. Niedermaier 《Naunyn-Schmiedeberg's archives of pharmacology》1981,315(3):255-267
Summary Partially purified chromaffin granules (granular fraction), crude mitochondria (mitochondrial fraction) and a microsomal fraction were prepared from bovine adrenals by differential centrifugation and characterized by their catecholamine content, succinate dehydrogenase and glucose-6-phosphatase activity. During isotonic incubation with 0.1 mM 45Ca2+ all fractions showed an uptake of 45Ca2+, which was stimulated by ATP. In addition, after incubation the granular fraction was further fractionated by sucrose density gradient centrifugation (2.0–1.3 M sucrose; 170,000g·60 min). In some of these experiments the granular fraction was incubated simultaneously with 45Ca2+ and [3H](-)noradrenaline to compare the uptake of both. The rate of uptake of 45Ca2+ into the 2.0 M sucrose fraction (characterized by the highest catecholamine content and the lowest succinate dehydrogenase activity) was doubled by ATP. The ATP-stimulated uptake of 45Ca2+ into the 2.0 M sucrose fraction of chromaffin granules was inhibited by N-ethylmaleimide (NEM) (0.1 mM), 2,4-dinitrophenol (DNP) (0.1 mM), azide (1 mM), carbonyl cyanide mchlorophenylhydrazone (CCCP) (20M), atractyloside (50 M), ruthenium red (40 M) and amobarbital (1 mM). This inhibition pattern was different from that of the ATP-stimulated uptake of 45Ca2+ into the microsomal fraction, but it was similar to that of the ATP-stimulated uptake of 45Ca2+ into the mitochondrial fraction. However, the following differences are incompatible with the view that a mitochondrial contamination, with a highly active uptake, is responsible for the ATP-stimulated uptake of 45Ca2+ into the 2.0 M sucrose fraction of chromaffin granules: a) The uptake of 45Ca2+ into the mitochondrial fraction was insensitive to 1 mM amobarbital, whereas this agent inhibited the uptake of 45Ca2+ into the 2.0 M sucrose fraction of chromaffin granules. b) Replacement of ATP by succinate stimulated the uptake of 45Ca2+ into the mitochondrial fraction only. c) The dependence of the ATP-stimulated uptake of 45Ca2+ on the concentration of ATP (0.1–5 mM) was determined: while uptake into the 2.0 M sucrose fraction of chromaffin granules exhibited saturation kinetics, that into the mitochondrial fraction was linearly related to the concentration of ATP. Interestingly, uptake of 45Ca2+ into those fractions of chromaffin granules that are known to be contaminated with mitochondria (1.6 M sucrose fraction) exhibited bot a saturable and a nonsaturable component. d) The uptake of 45Ca2+ into the mitochondrial fraction was more sensitive to 0.1 mM DNP than the uptake of 45Ca2+ into the 2.0 M sucrose fraction of chromaffin granules.The comparison of the ATP-stimulated uptake of 45Ca2+ with that of [3H](-)noradrenaline into the 2.0 M sucrose fraction of chromaffin granules revealed that amobarbital, N,N-dicyclohexylcarbodiimide (DCCD), DNP and increasing concentrations of ATP had the same influence on both uptake processes. From our results we conclude that an inherent, ATP-stimulated uptake of 45Ca2+ exists in chromaffin granules. The effects of agents on the uptake of 45Ca2+ and/or [3H](-)noradrenaline into chromaffin granules are discussed with regard to the granular uptake mechanisms.This work was supported by the Deutsche ForschungsgemeinschaftSome results were reported at the 18th Spring Meeting 1977 of the Deutsche Pharmakologische Gesellschaft (Burger and Häusler 1977) 相似文献
94.
Primary infiltrating signet ring carcinoma of the eyelids 总被引:1,自引:0,他引:1
F A Jakobiec P Austin T Iwamoto S L Trokel M D Marquardt W Harrison 《Ophthalmology》1983,90(3):291-299
A 61-year old man presented with a five-year history of a swelling initially developing in his right lower lid that progressed to involve the lateral canthal skin and eventually the upper lid and anterior orbit. He was discovered to have an infiltrating, poorly differentiated, mucin-producing carcinoma. Systemic work-up failed to disclose a visceral malignancy, and it was concluded that his tumor was primary in the lids, arising from an adnexal sweat gland. Three other reports in the literature also share almost identical clinical and pathologic features, in that all of the earlier reports dealt with middle-aged or elderly men who had diffusely indurated lids. Histopathologically, the tumor cells grow diffusely in a sclerotic stroma, and resemble the "histiocytoid" variant of metastatic breast carcinoma to the lids in women. Ultrastructural studies in our case point toward an apocrine origin, although earlier authors have favored an eccrine origin. Despite its indolent clinical course, the tumor is capable of producing regional and distant metastases on long-term follow-up. Complete local excision, possibly necessitating radical surgery, is probably the preferred method of treatment, but local radiotherapy may have a beneficial effect in retarding spread of the disease. 相似文献
95.
The Merkel cell is a distinctive nondendritic, nonkeratinocytic, epithelial clear cell believed to migrate from the neural crest to the epidermis and dermis, which is usually located in or near the basal layer of the epidermis and associated with nerve terminations. Merkel first described these cells in 1875 as "Tastzellen" occurring in the snout of a mole. They are believed to function as slowly adapting mechanoreceptors that mediate the sense of touch. Tumors arising from Merkel cells have been reported to occur on the head and neck area, the trunk, arms, and legs, and resemble a primary cutaneous lymphoma or cutaneous metastasis of a lymphoma or a carcinoma. Electron microscopy, to locate the characteristic membrane-bound, dense core neurosecretory granules, is needed for accurate diagnosis. These tumors must be treated aggressively to minimize the chance of local recurrence and nodal or visceral metastases. The authors present a case of Merkel cell tumor occurring on the eyelid. The clinical history, light and electron microscopic findings are shown. 相似文献
96.
板蓝根醇沉物的药理作用 总被引:5,自引:0,他引:5
目的:观察板蓝根醇沉物与板蓝根颗粒剂的差异。方法:对比研究板蓝根醇沉物与板蓝根颗粒剂前浸膏的药理作用。结果:板蓝根醇沉物的热解、抗炎及抗茵作用与同剂量板蓝根颗粒剂前浸膏相同,其靛蓝含量高于板蓝根颗粒剂前浸膏。结论:板蓝根醇沉物有明显的解热、抗炎作用,板蓝根制剂传统制备工艺有待改进。 相似文献
97.
尿石通颗粒中总黄酮的含量测定 总被引:2,自引:0,他引:2
目的:测定尿石通颗粒中总黄酮的含量。方法:用70%乙醇提取并制成供试品,采用分光光度法测定。结果:平均回收率为98.28%,RSD=2.13%。3批尿石通颗粒样品中总黄酮的含量分别为1.448,1.403,1.427 mg·g~(-1)。结论:本方法简便、准确、灵敏度高,可作为该制剂的质量控制方法。 相似文献
98.
99.
100.
目的 观察吡格列酮治疗 2型糖尿病的临床疗效。 方法 采取随机双盲对照临床研究 ,在应用吡格列酮治疗前及用药后第 12周分别进行空腹血糖、糖化血红蛋白的监测 ,同时检测血常规、肝功能、肾功能、血脂、血压。 结果 吡格列酮组空腹血糖 ,糖化血红蛋白在用药后显著下降 (P <0 .0 1) ,对肝功能、肾功能、凝血功能无影响 ,也未发生过敏反应 ,疗效吡格列酮组显著优于安慰剂组。 结论 吡格列酮能提高胰岛素敏感性 ,显著降低 2型糖尿病空腹血糖及糖化血红蛋白 ,是治疗 2型糖尿病的一种新型降血糖药。 相似文献