补肾益精法对硬皮病成纤维细胞表型的影响及其表观遗传学意义

目的：研究补肾益精法对硬皮病成纤维细胞表型的影响。方法：体外培养建立硬皮病成纤维细胞系，制作补肾益精法含药血清干预硬皮病成纤维细胞，使用实时定量RT—PCR技术，检测胶原表达相关因子及甲基化相关基因的表达情况，比较补肾益精法与5-氮杂-2-脱氧胞苷对硬皮病成纤维细胞的作用。结果：补肾益精法可抑制硬皮病成纤维细胞I型胶原α2（Coila2）、转化生长因子B（TGF-β）基因的表达，与5-氮杂-2-脱氧胞苷均可提高胶原抑制因Flil与Smad7表达，补肾益精法可减少DNA甲基转移酶1的表达。结论：补肾益精法可改善硬皮病成纤维细胞促纤维化的表型，其过程可能有表观遗传调控机制参与。     

Mortality in the rheumatic diseases

Objective. To review mortality data in published studies of various rheumatic diseases. Methods. A MEDLINE search of the literature on the rheumatic diseases, including osteoarthritis, rheumatoid arthritis, ankylosing spondylitis, systemic lupus erythematosus, scleroderma, polymyositis, and vasculitis. Results. Mortality rates higher than expected have been reported in most rheumatic conditions, considerably higher for inflammatory rheumatic diseases. The mortality rates in patients with systemic lupus erythematosus, scleroderma, polymyositis, and vasculitis are often comparable to mortality rates seen in patients with neoplastic or cardiovascular diseases, although the causes of death often are not identified as the rheumatic disease. Conclusion. Mortality has been found to be predicted in most instances by more severe clinical status, and therefore death should not be considered as “unrelated” to the rheumatic disease. These observations may have important implications for clinical care and health policies regarding patients with rheumatic diseases.