抱树莲生药学研究

目的：通过对抱树莲的生药学研究,为抱树莲的开发利用、质量标准的制定提供科学依据。方法：采用性状鉴定、显微鉴定、理化鉴定的方法进行研究。结果：抱树莲性状、显微、理化特征明显。结论：研究结果可作为抱树莲生药质量标准制定的依据。     

中药寒热预测系统的建立

中药寒热性质是中药药性的重要特点之一,作者在本文着重介绍了构建的中药寒热性质预测系统,并对中药寒热药性的化学基础进行了探讨.作者建立的寒热预测系统对中药寒热药性的预测准确率,训练集达到83.3%,测试集达到81.0%.在此预测模型的基础上,提出了原子数、氢键受体数、电负性表面面积等5个化学描述符,用于解释中药寒热药性的化学基础.     

噻唑橙光化学法病原体灭活对红细胞质量的影响

目的评价噻唑橙(TO)光化学法病原体灭活处理对红细胞质量的影响。方法设实验组(n=10):悬浮红细胞经TO光化学法处理后,在4℃储存0、7、14、21、28、35 d时,分别测定pH、溶血率、钾浓度、ATP浓度、2,3-DPG浓度;对照组(n=10):悬浮红细胞未经TO光化学法处理,测定时间、项目同实验组。结果保存过程中实验组pH、2,3-DPG(μmol/gHb)及ATP(μmol/gHb)下降趋势均与对照组差异不大(P0.05),实验组分别从0 d的7.03±0.07、9.43±1.14、4.23±0.58下降到35 d的6.57±0.06、0、2.40±0.49,对照组分别从0 d的6.95±0.09、10.67±1.17、5.18±0.64,下降到35 d的6.57±0.07、0、2.99±0.44;实验组和对照组35 d储存期末ATP浓度分别为0 d时的56.7%和57.7%。;实验组在储存期末,溶血率为(0.27±0.06)%,略高于对照组0.11±0.04%(P0.05),仍满足溶血率≤0.8%的国标要求;钾外漏实验组较对照组增加,35 d时分别为(40.41±2.94)mmol/L和(28.33±3.34)mmol/L(P0.01)。结论 TO光化学法处理对储存过程中的红细胞的质量影响较小。     

40种中药脂类含量与中药寒热性相关性研究

目的：探讨中药寒热药性与游离脂含量的相关性。方法：选取20种寒性中药和20种热性中药,提取总游离脂,运用统计学方法分析总游离脂含量与寒热药性的相关性。结果：热性中药的总脂含量明显高于寒性中药,两者比较,具有统计学意义（P〈0．05）。结论：中药总脂含量与中药药性存在相关性,脂类是中药寒热药性的物质基础之一。     

cDNA cloning, structural, and functional analyses of venom phospholipases A2 and a Kunitz-type protease inhibitor from steppe viper Vipera ursinii renardi

Snake venom phospholipases A₂ (PLA₂s) display a wide array of biological activities and are each characteristic to the venom. Here, we report on the cDNA cloning and characterization of PLA₂s from the steppe viper Vipera ursinii renardi venom glands. Among the five distinct PLA₂ cDNAs cloned and sequenced, the most common were the clones encoding a basic Ser-49 containing PLA₂ (Vur-S49). Other clones encoded either ammodytin analogs I1, I2d and I2a (designated as Vur-PL1, Vur-PL2 and Vur-PL3, respectively) or an ammodytoxin-like PLA₂ (Vurtoxin). Additionally, a novel Kunitz-type trypsin inhibitor for this venom species was cloned and sequenced. Comparison of these PLA₂ and Kunitz inhibitor sequences with those in the sequence data banks suggests that the viper V. u. renardi is closely related to Vipera ammodytes and Vipera aspis. Separation of V. u. renardi venom components by gel-filtration and ion-exchange chromatography showed the presence of many PLA₂ isoforms. Remarkably, the most abundant PLA₂ isolated was Vur-PL2 while Vur-S49 analog was in very low yield. There are great differences between the proportion of cDNA clones and that of the proteins isolated. Two Vur-PL2 isoforms (designated as Vur-PL2A and Vur-PL2B) indistinguishable by masses, peptide mass fingerprinting, N-terminal sequences and CD spectroscopy were purified from the pooled venom. However, when rechromatographed on cation-exchanger, Vur-PL2A showed only one peak corresponding to Vur-PL2B, suggesting the existence of conformers for Vur-PL2. Vur-PL2B was weakly cytotoxic to rat pheochromocytoma PC12 cells and showed both strong anticoagulant and anti-platelet activities. This is the first case of a strong anticoagulating ammodytin I analog in Vipera venom.     

Sexual Modes Questionnaire (SMQ): Translation and Psychometric Properties of the Italian Version of the Automatic Thought Scale

Background

The Sexual Modes Questionnaire (SMQ) is a validated and widespread used tool to assess the association among negative automatic thoughts, emotions, and sexual response during sexual activity in men and women.

Psychometric Properties of the Sexual Event Diary in a Sample of Dutch Women With Female Sexual Interest/Arousal Disorder

Background

Efficacy of on-demand drugs for women with hypoactive sexual desire disorder or female sexual interest/arousal disorder (FSIAD) should be assessed using a validated instrument that assesses the discrete sexual events during which the on-demand drug is taken, because this type of assessment is more proximate to an on-demand drug's efficacy compared to instruments that assess sexual function over longer periods of time.

檵木叶的形态解剖学研究

目的:为檵木叶的质量控制提供依据。方法:按生药学常规方法对檵木叶进行了形态组织学研究。结果:找出了檵木叶在药材性状、组织构造上的主要鉴别特征。结论:形态组织学特征可作为檵木叶的鉴定依据,从而为檵木叶的质量控制打下基础。     

Development of receptive field properties of retinal ganglion cells in kittens raised with a convergent squint

Summary The effects on retinal ganglion cell receptive fields of rearing kittens with convergent squint, surgically induced on the 12th post-natal day, were investigated by utilizing the extracellular single unit recording technique. The data revealed that responses of cross-eyed cat ganglion cells to contrast reversal stimuli were severely depressed and the retinal region exhibiting the best responses varied according to the degree of convergent misalignment of the eyes displayed by each animal. Receptive field sizes of X-type (but not Y-type) units located within 10 ° of the area centralis of cross-eyed cats were significantly larger than those in normally reared cats. Finally, the encounter rate for units exhibiting non-linearity of spatial summation (Y-type) were much lower in cross-eyed cats. The results suggest developmental alterations in the retinal neurophysiology of common cats reared with a large convergent squint.This investigation was supported by NIH grants EY01444 and EY00701     

影响中药双向调节作用的因素

讨论了中药双向调节作用的机理及影响因素，认为中药的双向调节作用与机体的病理体质，中药的化学万分有关，并受到中药的炮制、剂量、药用部位、配伍等因素的影响。