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991.
Summary A semi-quantitative procedure is described for measuring cell viability after short-term exposure to a test substance using
a monolayer culture. Test substances are placed in direct contact with cell monolayers for various time intervals. The substances
are removed and the monolayers are incubated in the presence of fluorescein diacetate. Monolayers are viewed under a fluorescent
microscope and the percentage of fluorescing (viable) cells is estimated. The method is suitable for examining cytotoxic effects
at short times of exposure and for discriminating between test substances that give similar, low toxicity endpoints in standard
24-h assays. 相似文献
992.
Maria Cristina Locatelli Lucilla Tedeschi Maurizia Clerici Antonella Romanelli Adelaide D'Antona Roberto Labianca Gino Luporini 《Supportive care in cancer》1996,4(3):218-219
We have reviewed the incidence of cisplatin-induced anaemia in patients affected with solid tumours treated with at least three courses of first-line cisplatincontaining regimens. In our experience, a low percentage (5%) of patients required transfusions of red blood cells. We think it is of the utmost importance to adopt uniform criteria in monitoring and treatment of patients at risk of developing cisplatin anaemia and to identify subsets of patients to eventually treat with erythropoietin. 相似文献
993.
脂蛋白(a)对肾小球系膜细胞的作用 总被引:21,自引:0,他引:21
目的探讨脂蛋白(a)[Lp(a)]对肾小球系膜细胞(GMC)的作用。方法将体外培养的系膜细胞,加脂蛋白(a)刺激后,测定细胞生长率和细胞上清中血小板活化因子(PAF)、肿瘤坏死因子(TNF)、乳酸脱氢酶(LDH)及纤维连结蛋白(FN)水平,并以未经刺激者作对照。结果经脂蛋白(a)刺激48小时,在终浓度5~20mg/L时细胞生长率轻度增加,而在50~400mg/L时细胞生长率明显下降。刺激18小时,细胞上清中PAF的水平明显高于对照,且与脂蛋白(a)浓度呈明显正相关(r=0.937,P<0.01);L929细胞的杀伤率有所增加,但未达50%;LDH的水平在脂蛋白(a)100mg/L以下时有所增加;刺激18、48、72小时后的细胞上清纤维连结蛋白均为阴性。结论脂蛋白(a)对系膜细胞体外增殖有低浓度轻度促进、高浓度抑制的双向作用,对细胞PAF的产生具有浓度依赖的促进作用,并能增加细胞上清中LDH及TNF水平,因而可通过多种途径介导肾小球损伤。 相似文献
994.
补肾中药对雄激素致不孕大鼠卵巢核仁组成区蛋白的影响 总被引:4,自引:0,他引:4
观察补肾中药对雄激素致不孕大鼠卵巢核仁组成区蛋白的影响。用SD雌性大鼠幼仔,出生9d龄注射丙酸睾丸酮,制成雄激素致不孕大鼠(ASR)模型。80d龄灌服补肾中药水溶液两周。100d龄心脏灌注处死。以核仁组成区蛋白嗜银染色(AgNOR)和增殖细胞核杭原(PcNA)为指标,观察卵巢颗粒细胞、间质腺细胞的形态学变化。结果:补肾中药能够使ASR卵巢颗粒细胞增殖、卵泡发育。治疗组的AgNOR和PcNA计数明显高于模型组,而与对照组之间无显著差异。结论:补肾中药的这种作用可能是通过调节了下丘脑-垂体-卵巢性腺功能的结果,从而促使卵巢颗粒细胞发育、卵泡成熟。 相似文献
995.
Jan E. Zejda Thomas S. Hurst Ernest M. Barber Charles Rhodes James A. Dosman 《American journal of industrial medicine》1993,23(5):743-750
A cross-sectional survey on respiratory health in swine producers showed that 30% of 301 examined men usually used a dust mask when working inside a barn. They did not differ significantly from dust mask nonusers in respect to respiratory symptoms and lung function. This analysis was undertaken to determine whether the respiratory health of dust mask users was associated with reasons why they had started individual respiratory protection. The subjects were recontacted in order to identify those who started using a mask to deliberately prevent symptoms (42 men) and those who started protection because of pre-existing respiratory symptoms (44 men). Not unexpectedly, betweengroup comparisons of respiratory symptoms and lung function suggest that swine producers who wear dust masks for preventive purposes have better respiratory health than those who wear dust masks because of symptoms or those who do not use individual respiratory protection. The individual reasons for starting dust mask usage should be examined among potential determinants of the outcomes of prospective studies which can then provide more valid assessment of the effect of individual respiratory protection. © 1993 Wiley-Liss, Inc. 相似文献
996.
997.
用刀豆蛋白A刺激激素敏感型肾病单个核细胞,并将其培养上清液灌注于大鼠肾内。结合电镜观察,用图像分析仪及体视学方法分别对其肾组织胶体铁染色切片和电镜照片上的足突和滤过裂隙的平均宽度进行测定。结果表明:该病极期上清液灌注的鼠肾小球多阴离子明显减少,足突肿胀、融合,滤过裂隙变窄;而该病缓解组和正常对照组则为阴性。说明该病单个核细胞培养上清液可引起鼠肾小球微小病变型肾病综合征的病变,并揭示该变化可能是由其上清液中一种异常的T淋巴细胞因子所致。 相似文献
998.
Peter M. Ravdin Stephanie Green James H. Doroshow Silvana Martino 《Investigational new drugs》1994,12(4):333-336
Summary Thirty-two eligible patients with advanced metastatic breast cancer who had received no more than 1 prior chemotherapy regimen for metastatic disease (16 had received prior doxorubicin) were treated with piroxantrone at a dose of 120 mg/m2 intravenously every 21 days. In the twenty-seven patients evaluable for response, two partial responses were seen. Toxicities observed were primarily hematologic with grade 3 or greater granulocytopenia occurring in 34% of the patients. One patient developed symptomatic congestive heart failure at a total cumulative dose of 960 mg/m2. We conclude that piroxantrone given at this dose and schedule has minimal activity in patients with metastatic breast cancer. 相似文献
999.
Robert C. Flanigan Joseph H. Saiers Michael Wolf Eric H. Kraut Anthony Y. Smith Brent Blumenstein E. David Crawford 《Investigational new drugs》1994,12(2):147-149
Summary The Southwest Oncology Group (SWOG) studied the response rate and toxicity of merbarone (1,000 mg/m2 IV continuous infusion days 1–5, q 21 days) in patients with advanced metastatic renal cell carcinoma. Among 36 eligible patients, there was one partial response for a response rate of 3% (95% C.I. 0.1–15%). There were no mixed responses. There were no treatment related deaths or adverse drug reactions. Significant anemia, diarrhea, and hypercalcemia were observed. Mild to moderate degrees of malaise/fatigue/lethargy, dizziness/vertigo, hyperglycemia, creatinine increase, nausea, vomiting, weight loss, pedal edema, dyspnea, and granulocytopenia were noted. Merbarone does not have significant activity as a single agent in advanced renal cell carcinoma. 相似文献
1000.
Summary Based upon the hypothesis that dipyridamole would potentiate the cytotoxicity of mitoxantrone and the combination of 5-fluorouracil (5-FU) and leukovorin, we performed a phase I/II trial of the combination of dipyridamole, 5-FU, leukovorin, and mitoxantrone in patients with metastatic breast cancer. The dose of dipyridamole was fixed at 175 mg/m2 by mouth every 6 h (700 mg/m2/day), based upon a previous phase I trial of oral dipyridamole with 5-FU and leukovorin. Dipyridamole therapy began 24 h prior to the first dose of chemotherapy and continued until 24 h after the last dose of chemotherapy for each course of treatment. At the initial dose level, leukovorin 200 mg/m2 was given intravenously immediately prior to 5-FU 375 mg/ m2 intravenously on days 1–5. Mitoxantrone 6 mg/m2 was given as a single dose on day 3. Unacceptable toxicity was observed at this dose level, leading to successive dose decrements rather than dose increments. The maximum tolerated dose was leukovorin 200 mg/m2 days 1–2, 5-FU 375 mg/m2 days 1–2, mitoxantrone 6 mg/m2 on day 2, and dipyridamole 175 mg/m2 every 6 h on days 0–3. Two responses were produced in 15 patients. This regimen is not recommended for further investigation in the treatment of breast cancer. 相似文献