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101.
活动性结核标志物'H-多肽的实验与临床研究 总被引:2,自引:0,他引:2
本题研究是经免疫学途径直接检测人体感染结核菌的情况,为现代结核病的实验诊断、临床监测、流行病调查提供了一个全新的检验指标。作者首先发现了一种仅存在于活动性结核病患者体液中的蛋白成份—活动性结核标志物(ActiveTuberculosisMark—ATM)1H—多肽;并为之创立了独特的检测方法,经四年多临床19460例样本调查中确定了ATM的临床价值。将ATM检测与OT皮试、酶联免疫ELISA、DNA探针、PCR基因扩增技术及典型病例组患者行X线计算机断层摄影(CT)、磁共振像(MRI)等多组对比试验中,实验与临床研究资料分析证明:ATM检测的总敏感度为86.06%、特异度96.24%、准确度93.45%、诊断效率为82.82%、批内CV1.2%、批间CV2.0%、P<0.05。经NMR光谱分析结构含有CCH2官能团。 相似文献
102.
本文采用紫外分光光度法测定头孢呋辛钠的含量,测定波长274nm,线性范围为8~50ug/ml,相关系数r=0.9999(n=5),测得吸收系数(1%1cm)为396.回归方程法和吸收系数法的平均回收率分别为99.87和100.37,CV分别为0.47.0.38。该方法筒便、快速,结果准确。 相似文献
103.
AmpliSensor-聚合酶链反应技术检测结核分支杆菌及临床应用 总被引:14,自引:3,他引:11
目的探讨Amplisensor-聚合酶链反应(AmpliSensor-PCR)在结核病诊断中的价值。方法采用AmpliSensor-PCR对784例结核病患者及160例肺癌患者的标本进行检测,并与PCR(凝胶电泳后,经溴化乙锭染色)、涂片、培养等法比较。结果AmpliSensor-PCR的敏感性显著高于涂片及培养(P<0.01)。特异性较PCR法高。结论AmpliSensor-PCR可以通过标准曲线划定检出下限,并可换算出标本中原始的靶DNA值,同时具有较高的特异性和敏感性,对肺结核尤其是肺外结核的诊断有一定的临床意义。 相似文献
104.
本文调查了一个铀水冶厂放射性废物坑的污染情况,发现该坑周围农田及坑附近淠史杭大灌渠水、泥等均受到不同程度污染。这表明,即使处理小型铀水冶厂的放射性废物,在选址、掩埋等各个环节也都应严格做到科学、合理,并且还要做好掩埋后的管理工作。 相似文献
105.
Patterns of calcifications and cholangiographic findings in hepatobiliary tuberculosis 总被引:3,自引:0,他引:3
Dean D. T. Maglinte M.D. Sol Z. Alvarez Anastacio C. Ng Jose L. Lapeña 《Abdominal imaging》1988,13(1):331-335
The radiologic findings on conventional examinations (plain films and cholangiograms) in a large group of patients with proven hepatobiliary tuberculosis are reviewed. The plain film findings of large chalky and confluent hepatic calcifications or nodal-type calcifications along the course of the common bile duct are suggestive of hepatobiliary tuberculosis. Small, discrete, scattered calcifications may be mimicked by histoplasmosis but can be differentiated from hepatobiliary tuberculosis. Obstructing defects seen on cholangiography are indicative of tuberculosis when adjacent calcifications are present. The patterns of liver calcifications could provide a clue to the diagnosis of hepatobiliary tuberculosis and its differentiation from liver calcifications of various other etiologies. 相似文献
106.
目的探讨活动性肺结核(APTB)患者血清Fad D9重组蛋白、可溶性白细胞分化抗原14亚型(sCD14-ST)、单核细胞P糖蛋白(Pgp)表达及其临床意义。 方法选取96例APTB患者(APTB组),按照肺部病灶有无空洞和病灶肺叶数分亚组。同期选择体检健康且经γ干扰素释放试验检测阴性者(健康对照组,HC组)及阳性者(结核潜伏感染组,LTBI组)各48例。比较各组血清Fad D9重组蛋白、sCD14-ST、Pgp水平。评估Fad D9重组蛋白、sCD14-ST、Pgp诊断APTB和鉴别APTB、LTBI的价值。 结果Fad D9重组蛋白、sCD14-ST、Pgp水平APTB组>LTBI组>HC组,且有空洞者高于无空洞者,病灶肺叶≥3个者高于<3个者(P<0.05)。Fad D9重组蛋白、sCD14-ST、Pgp对APTB诊断和APTB、LTBI鉴别有良好效能(P<0.05)。 结论APTB患者血清Fad D9重组蛋白、sCD14-ST及Pgp明显升高,且与肺部病灶的严重程度有关。 相似文献
107.
108.
Herbert Nsanze Abdulmajeed S. Ameen Esther Fares Lalita Vargees Nada Mustafa 《Clinical microbiology and infection》1997,3(2):236-239
Objective: To evaluate the use of serodiagnosis for tuberculosis and leprosy using mycobacterial antigen 38 kDa, with kits from Omega laboratories, to detect IgG by enzyme immunoassay (EIA).
Method: The study population consisted of 58 patients with evidence of tuberculous infection (culture of Mycobacterium tuberculosis complex or microscopic evidence), of whom 23 had pulmonary and 35 had extrapulmonary disease. There were six subjects who had recently been treated for tuberculosis, 11 patients on treatment for leprosy and 137 patients suspected of having tuberculosis on clinical or radiologic grounds (without laboratory evidence). A control group comprised 35 healthy individuals or patients suffering from diseases other than tuberculosis.
Results: The tests showed that there was a significant difference in antibody levels between the patients with active pulmonary disease, extrapulmonary tuberculosis and leprosy in comparison with the control group ( p <0.001). The sensitivities of the two tests together for proven pulmonary tuberculosis were 100% and 95.7% at 1.0–1.5 and >1.6 EIA cut-off points respectively, while the specificities were 88.5% and 100% at the same cut-off points. The sensitivities for extrapulmonary tuberculosis were 71.4% and only 51.4% at 1.0–1.5 and >1.6 EIA cut-off points. The test was positive in 30 (21.9%) of the 137 suspected patients, while 43 (31.4%) had an equivocal result and the remaining 64 (47.7%) suspects were definitely negative. There was again a significant difference in positivity rates between suspects and the control group.
Conclusions: Omega IgG test is useful in the serodiagnosis of active pulmonary tuberculosis and leprosy, but less sensitive in extrapulmonary disease, particularly in children. Equivocal results may only add to the evidence of tuberculosis in early or minimal disease. 相似文献
Method: The study population consisted of 58 patients with evidence of tuberculous infection (culture of Mycobacterium tuberculosis complex or microscopic evidence), of whom 23 had pulmonary and 35 had extrapulmonary disease. There were six subjects who had recently been treated for tuberculosis, 11 patients on treatment for leprosy and 137 patients suspected of having tuberculosis on clinical or radiologic grounds (without laboratory evidence). A control group comprised 35 healthy individuals or patients suffering from diseases other than tuberculosis.
Results: The tests showed that there was a significant difference in antibody levels between the patients with active pulmonary disease, extrapulmonary tuberculosis and leprosy in comparison with the control group ( p <0.001). The sensitivities of the two tests together for proven pulmonary tuberculosis were 100% and 95.7% at 1.0–1.5 and >1.6 EIA cut-off points respectively, while the specificities were 88.5% and 100% at the same cut-off points. The sensitivities for extrapulmonary tuberculosis were 71.4% and only 51.4% at 1.0–1.5 and >1.6 EIA cut-off points. The test was positive in 30 (21.9%) of the 137 suspected patients, while 43 (31.4%) had an equivocal result and the remaining 64 (47.7%) suspects were definitely negative. There was again a significant difference in positivity rates between suspects and the control group.
Conclusions: Omega IgG test is useful in the serodiagnosis of active pulmonary tuberculosis and leprosy, but less sensitive in extrapulmonary disease, particularly in children. Equivocal results may only add to the evidence of tuberculosis in early or minimal disease. 相似文献
109.
Induction and expression of protective T cells during Mycobacterium avium infections in mice. 下载免费PDF全文
Mycobacterium avium is an opportunistic pathogen that infects individuals suffering from chronic lung disease or immunocompromised patients such as AIDS patients. Here we show that a highly virulent isolate of M. avium proliferated as extensively in T cell deficient as in immunocompetent mice. T cell deficient mice allowed a progressive growth of a less virulent AIDS-derived isolate of M. avium while immunocompetent mice arrested the growth of this isolate. Adoptive transfer of T cell enriched spleen cells between congenic strains of mice differing at the Bcg/Ity/Lsh locus showed that only naturally resistant BALB/c.Bcgr (C.D2) mice infected with the highly virulent strain of M. avium or the naturally susceptible BALB/c mice infected with the lower virulence isolate developed protective T cells and that these cells only mediated protection when transferred to naturally susceptible, but not to naturally resistant, mice. Both strains of M. avium proliferated in bone marrow-derived macrophages cultured in vitro and they were both susceptible to the bacteriostatic effects induced in the macrophages by crude lymphokines produced by concanavalin A-stimulated spleen cells. 相似文献
110.
The mode of action of treatment by IgG of haemolytic anaemia induced by an anti-erythrocyte monoclonal antibody 总被引:3,自引:0,他引:3 下载免费PDF全文
Y POTTIER I PIERARD A BARCLAY P L MASSON J-P COUTELIER 《Clinical and experimental immunology》1996,106(1):103-107
Tolerization of pathogenic antigens is one of the experimental strategies that has been proposed to prevent autoimmune disease. We have investigated here whether neonatal intraperitoneal infection of Lewis rats with Mycobacterium bovis-BCG has any effect on the expression of adjuvant arthritis (AA), an autoimmune disease that is produced by immunization of the rats with dead mycobacteria in mineral oil (i.e. Freund's complete adjuvant (FCA)). We found that neonatal infection with 108 viable BCG bacilli rendered all Lewis rats resistant to the expression of AA after FCA immunization. This BCG-induced protection from reactive arthritis was not seen in Lewis rats infected with smaller inocula (106 BCG bacilli) or if the infection was performed after the neonatal period (e.g. at 3 weeks of age). Neonatal administration of 65-kD mycobacterial heat shock protein (hsp65, a key antigen in the etiopathogenesis of AA) failed to protect Lewis rats from AA; injection of lactoferrin (an autoantigen that may be involved in the physiopathology of autoimmune arthritis) to newborn Lewis rats decreased the severity of AA observed after FCA immunization of the animals. Western blotting revealed that Lewis rats that had acquired resistance to AA also showed changes in their repertoire of antibody specificities; among these alterations was decreased anti-hsp65 reactivity. We conclude that neonatal infection with BCG, but not hsp65 injection, renders Lewis rats resistant to AA and that the phenomenon is associated with change in the repertoire of specificities of circulating antibodies. 相似文献