全文获取类型
收费全文 | 142087篇 |
免费 | 10554篇 |
国内免费 | 5983篇 |
专业分类
耳鼻咽喉 | 664篇 |
儿科学 | 2474篇 |
妇产科学 | 1512篇 |
基础医学 | 21083篇 |
口腔科学 | 2311篇 |
临床医学 | 12019篇 |
内科学 | 28206篇 |
皮肤病学 | 1937篇 |
神经病学 | 11395篇 |
特种医学 | 3451篇 |
外国民族医学 | 19篇 |
外科学 | 9644篇 |
综合类 | 21210篇 |
现状与发展 | 29篇 |
预防医学 | 9084篇 |
眼科学 | 2200篇 |
药学 | 18899篇 |
29篇 | |
中国医学 | 4601篇 |
肿瘤学 | 7857篇 |
出版年
2023年 | 1467篇 |
2022年 | 2479篇 |
2021年 | 4646篇 |
2020年 | 4053篇 |
2019年 | 4243篇 |
2018年 | 4229篇 |
2017年 | 4427篇 |
2016年 | 4737篇 |
2015年 | 5294篇 |
2014年 | 8929篇 |
2013年 | 10058篇 |
2012年 | 9086篇 |
2011年 | 9954篇 |
2010年 | 7815篇 |
2009年 | 7402篇 |
2008年 | 7546篇 |
2007年 | 7528篇 |
2006年 | 6703篇 |
2005年 | 6088篇 |
2004年 | 5160篇 |
2003年 | 4488篇 |
2002年 | 3570篇 |
2001年 | 3096篇 |
2000年 | 2691篇 |
1999年 | 2355篇 |
1998年 | 2075篇 |
1997年 | 1974篇 |
1996年 | 1643篇 |
1995年 | 1420篇 |
1994年 | 1283篇 |
1993年 | 1010篇 |
1992年 | 896篇 |
1991年 | 780篇 |
1990年 | 641篇 |
1989年 | 533篇 |
1988年 | 495篇 |
1987年 | 475篇 |
1986年 | 408篇 |
1985年 | 895篇 |
1984年 | 933篇 |
1983年 | 706篇 |
1982年 | 709篇 |
1981年 | 629篇 |
1980年 | 561篇 |
1979年 | 459篇 |
1978年 | 386篇 |
1977年 | 306篇 |
1976年 | 334篇 |
1975年 | 314篇 |
1974年 | 249篇 |
排序方式: 共有10000条查询结果,搜索用时 234 毫秒
991.
992.
Effect of ozone exposure on allergic sensitization and airway inflammation induced by dendritic cells 总被引:2,自引:0,他引:2
P. O. Depuydt B. N. Lambrecht G. F. Joos R. A. Pauwels 《Clinical and experimental allergy》2002,32(3):391-396
BACKGROUND: Epidemiological studies suggest that ozone exposure is related to increased asthma symptoms. Dendritic cells (DCs) are the principal antigen-presenting cells in the airways. OBJECTIVE: We have examined whether ambient doses of ozone (100 ppb for 2 h) enhance allergic sensitization and/or airway inflammation in a mouse model. METHODS: C57BL/6 mice were sensitized to inhaled ovalbumin (OVA) by intratracheal instillation of OVA-pulsed DCs on day 0. Daily exposure to OVA aerosol on days 14-20 resulted in an eosinophilic airway inflammation, as reflected in bronchoalveolar lavage fluid and lung histology. In a first experiment, mice were exposed to ozone or room air immediately prior to and following sensitization. Subsequently, we tested the effect of ozone exposure during antigen challenge in DC-sensitized mice. RESULTS: Exposure to ozone during sensitization did not influence airway inflammation after subsequent allergen challenge. In contrast, in sensitized mice, challenge with OVA together with ozone (days 14-20) resulted in enhanced airway eosinophilia and lymphocytosis, as compared with mice exposed to OVA and room air (1.91 x 106 +/- 0.46 x 106 vs. 0.16 x 106 +/- 0.06 x 106 eosinophils/mL lavage fluid; P = 0.015; 0.49 x 106 +/- 0.11 x 106 vs. 0.08 x 106 +/- 0.03 x 106 lymphocytes/mL lavage fluid; P = 0.004). Ozone exposure without subsequent OVA exposure did not cause airway inflammation. CONCLUSION: Ozone exposure does not increase allergic sensitization but enhances antigen-induced airway inflammation in mice that are sensitized via the airways. 相似文献
993.
Laboratory diagnosis of variant Creutzfeldt-Jakob disease 总被引:1,自引:0,他引:1
The neuropathological and biochemical features of 33 cases of variant Creutzfeldt-Jakob disease (vCJD) diagnosed up to the end of 1998 are analysed in relation to the 646 cases of suspected CJD referred to the CJD Surveillance Unit laboratory from 1990 to 1998. Morphological studies of the central nervous system, lymphoid tissues and other organs were accompanied by immunocytochemistry; Western blot analysis of PrPRES was performed on frozen brain tissue. The findings were analysed in relation to clinical and genetic data. The pathology of vCJD showed morphological and immunocytochemical characteristics distinct from other cases of CJD. PrP accumulation was widespread in lymphoid tissues in vCJD, but was not identified in other non-neural tissues. PrPRES accumulation in vCJD brain tissue showed a uniform glycotype pattern distinct from sporadic CJD. All analysed cases of vCJD were methionine homozygotes at codon 129 of the PrP gene. No evidence currently exists to suggest that cases of CJD diagnosed in individuals who are MV or VV at codon 129 of the PrP gene represent 'human bovine spongiform encaphalopathy (BSE)'. Continued surveillance is required to further investigate this possibility, with the need to investigate autopsy tissues from suspected cases by histological and biochemical techniques. 相似文献
994.
Bath application of the inhibitors of phospholipases, nordihydroguaiaretic acid (NDGA) and p-bromophenacyl bromide (BPB), to the rat hippocampal slices suppressed long-term potentiation (LTP) in Schaffer/commissural-CA1 pyramidal synapses. On the other hand, neither of the two inhibitors suppressed LTP in mossy fiber-CA3 pyramidal cell synapses. BPB did not suppress phosphatidylinositol-specific phospholipase C (PI-PLC) activity of the slices. These results suggested that the mechanisms of LTP were quite different in the CA1 and CA3 subfields of rat hippocampus: in CA1, the involvement of an arachidonate metabolism was strongly suggested, whereas in CA3, an arachidonic acid cascade may not be necessary for LTP. 相似文献
995.
996.
D. Dory C. Chopin I. Aimone-Gastin J. L. Gueant L. Guerin J. Sainte-Laudy D. A. Moneret-Vautrin J. Fleurence 《Allergy》1998,53(1):42-50
Allergy to fish is one of the most common food allergies. Gad c 1 is the only fish allergen which has been purified and characterized. Other allergens have been detected by Western blot in cod extracts. We have now improved the Western-blot procedure in order to characterize fish IgE-reactive proteins from extracts prepared under different conditions: pre-rigor mortis and postrigor mortis. EDTA addition or not. and DEAE ion-exchange chromatography. Several IgE-reactive protein bands have been identified over a wide molecular-weight range. In particular, the 104- and 130-kDa IgEreactive protein bands were detected. These new bands may correspond to aggregates, as EDTA increased the relative amount of the 60-, 67-, 104-, and 130-kDa IgE-reactive protein bands in Western blot. All these bands were also detected by an antiparvalbumin monoclonal antibody, specific to the first calcium-binding site. The longer period of storage increased the relative amounts of the 41-, 80-, 104-. and 130-kDa IgE-reactive protein bands. The 18-kDa band was detected only in fish stored for several days. In conclusion, we have described IgE-reactive protein bands over a wide molecular-weight range (12–130 kDa) in Western blot of cod extract, and shown that EDTA and storage conditions may influence the relative distribution of IgE-reactive protein bands. 相似文献
997.
998.
Z. Oláh S. Komoly N. Nagashima F. Joó U. R. Rapp W. B. Anderson 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1991,83(2):403-410
Summary In this report we describe changes in the intracellular redistribution of raf serine/threonine protein kinase (product of the raf proto-oncogene family) in hippocampal neurons following cerebral ischemia in Mongolian gerbils. For immunohistochemical localization studies polyclonal antisera specific for each of the A, B, and Raf-1 isotypes of raf, as well as a pan-raf antisera, were employed. Of these, only sera recognizing B-raf, as well as the general v-raf (raised against the conserved C-terminal region) were positive, indicating that B-raf is the major isotype in this neuronal region. Three different ischemie models were used (repeated 3 times for two min and single 5 or 15 min occlusions, of the common carotid arteries) to demonstrate that ischemie insult causes redistribution of raf protein kinase into the cell nucleus of hippocampal neurons. Increased amounts of raf protein in the nuclei of pyramidal cells following ischemia was confirmed by Western blot analysis of isolated nuclear fractionations. Moreover, an elevation in the level of nuclear raf protein also was detected in the contralateral (i.e. non-occluded hemisphere) neurons of CA1 and CA3 subfields 4 days after the ischemie insult indicating a possible transsynaptic increase in the amount of raf protein along with redistribution. The intranuclear translocation of the immunoreactive material started from the perinucleolar rim and with time extended throughout the nucleus. Enhanced levels and altered redistribution of the raf polypeptide in the nuclei of pyramidal cells of the CA3 subfleld appears to be reversible and returns to the normal level 12 days following the ischemic insult. In addition to triggering the above changes in the intracellular redistribution of raf, ischemie insult also caused an increase in the level of B-raf protein in reactive astrocytes. 相似文献
999.
Raymond G. Goodwin Wenie S. Din Terri Davis-Smith Dirk M. Anderson Steven D. Gimpel Timothy A. Sato Charles R. Maliszewski Camilynn I. Brannan Neal G. Copeland Nancy A. Jenkins Terry Farrah Richard J. Armitage William C. Fanslow Craig A. Smith 《European journal of immunology》1993,23(10):2631-2641
4-1BB is an inducible T cell antigen that shows sequence homology to members of an emerging family of cytokine receptors, including those for tumor necrosis factor and nerve growth factor. To aid in the analysis of the function of 4-1BB we have utilized a soluble form of the molecule as a probe to identify and clone the gene which encodes its ligand. The ligand for 4-1BB is a type II membrane glycoprotein that has homology to tumor necrosis factor, lymphotoxin, and the ligands for CD40 and CD27, all of which are themselves ligands to receptors in this superfamily. The gene for 4-IBB is on mouse chromosome 4 and maps close to the p80 form of the tumor necrosis factor receptor as well as the gene for CD30. The gene for 4-IBB ligand maps to mouse chromosome 17, but considerably distal to the tumor necrosis factor and lymphotoxin genes. Interaction of 4-1BB with its ligand induces the proliferation of activated thymocytes and splenic T cells, a response which is mimicked on similar cell populations stimulated with an antibody to 4-1BB. 相似文献
1000.
目的探讨高压电烧伤对家兔血液流变学的影响及已酮可可碱对血液流变学异常的改善作用。方法将40只家兔随机分为高压电烧伤组(电伤组)、高压电烧伤复合已酮可可碱治疗组(复合组),每组20只。采用BV-100型悬丝生物流变仪,检测两组家兔伤前1 h、伤后即刻、伤后24 h、伤后48 h共四个时相的血液流变学指标,指标包括全血黏度(ηb)、全血还原黏度(ηr)、血浆黏度(ηp)、血沉(ESR)、血沉方程K值(K)、红细胞比容(HCT)、纤维蛋白原(FB)、红细胞聚集指数(EAI)、红细胞刚性指数(TK),对检测结果进行统计学分析。结果1、组内比较:电伤组和复合组ηb、ηr和EAI伤后各时相值均较伤前1 h升高(P<0.05);ηp、ESR、K、HCT、FB均从伤后24 h升高,24、48 h与伤前1 h比较均有显著性差异(P<0.05);电伤组TK于24、48 h升高,与伤前1 h比较均有显著性差异,而复合组TK伤后各时相与伤前比较无显著性差异(P>0.05)。2、组间同时相比较:复合组伤后各时相ηb和ηr均低于电伤组(P<0.05);复合组伤后24、48 h两个时相的ηp、ESR、K、HCT、FB、EAI、TK均低于电伤组(P<0.05)。结论高压电烧伤可引起家兔血液流变学异常,已酮可可碱对高压电引起的血液流变学异常有改善作用。 相似文献