Growth inhibition of estrogen independent MXT mouse mammary carcinomas in mice treated with an agonist or antagonist of LH-RH,an analog of somatostatin,or a combination

Summary Female BDF₁ mice inoculated with MXT (3.2) estrogen independent mouse mammary carcinoma were treated for three weeks with microcapsules of the luteinizing hormone-releasing hormone (LH-RH) agonist [D-Trp⁶]LH-RH, the antagonist SB-75, the somatostatin analog RC-160, or combinations. The lack of estrogen dependence of the tumor was proved by bilateral surgical ovariectomy, which had no effect. In two experiments, treatment with 25µg/day doses of each analog alone resulted in a significant inhibition of tumor growth as shown by a 40–53% inhibition of tumor volumes, 38–43% decrease in tumor weights, and histological signs of tumor regression. However, the combination of SB-75 or [D-Trp⁶]LH-RH with somatostatin analog RC-160 caused greater reduction of tumor volume (68 and 61%) or tumor weights (59 and 56%), than single analogs, and histologically the occurrence of apoptosis and decrease in AgNOR numbers was more pronounced in the groups receiving combination therapy. Specific binding sites for [D-Trp⁶]LH-RH, EGF, and IGF-I were demonstrated in the tumor membranes. The binding capacity of LH-RH receptors was decreased by treatment with the analogs, the greatest down-regulation being caused by combination therapy. A significant decrease in EGF binding capacity was observed after treatment with the LH-RH analogs, alone or especially in combination with somatostatin analog RC-160. The combination of these analogs also caused a reduction in IGF-I receptors. The finding that LH-RH agonists and antagonists and somatostatin analogs inhibit the growth of estrogen independent mammary tumors, and that combinations are more effective than single analogs, might be of practical importance in human breast cancer therapy.     

冰蜜生肌膏对兔成纤维细胞增殖影响的实验研究

目的:观察冰蜜生肌膏促进体外培养兔成纤维细胞增殖的影响。方法:采用消化分离法进行兔成纤维细胞的原代培养,通过形态学和免疫组织化学的方法鉴定成纤维细胞。第3代纯化的成纤维细胞分为5组,空白对照组每孔加入等量的PBS,实验组每孔分别加入125、62·5、31·25、15·625mg/ml的冰蜜生肌膏提取液进行培养,培养24、48、72h后分别行MTT法检测每孔的光吸收值(OD值)。结果:冰蜜生肌膏提取液加入到成纤维细胞中培养24、48、72h后,经MTT法检测,不同浓度(浓度分别为125、62·5、31·25、15·625mg/ml)冰蜜生肌膏与对照组OD值比较差异有显著性统计学意义(P<0·05)。结论:15·625mg/ml浓度冰蜜生肌膏对成纤维细胞的生长有明显促进作用(P<0·05),药物浓度在15·625~62·5mg/ml时其作用随药物浓度升高而增加,并在浓度为62·5mg/ml时达到最大效应值(P<0·01),当药物浓度大于62·5mg/ml时则作用趋向于饱和,提示一定浓度范围内的冰蜜生肌膏药液能明显增加成纤维细胞数量,且促进作用和浓度呈显著依赖性。     

雌激素对内膜癌细胞中FAK基因转录的影响

目的定量观察雌激素刺激后内膜癌细胞的焦点粘连激酶（Focal—adhesion kinase，FAK）转录水平。方法不同浓度的17β-雌二醇体外刺激雌激素敏感的内膜癌细胞株RL95—2。加用三苯氧胺或ICI 182，780雌激素拮抗剂的细胞及未加雌激素的细胞设为阴性对照。经不同作用时间后，采用实时RT—PCR方法测定处理后的细胞内FAK的mRNA水平。结果在给予不同浓度的雌激素共培养1d或5d后，细胞的FAK基因的转录水平对比阴性对照细胞无明显改变。结论雌激素对内膜癌细胞的FAK转录可能无显著影响。FAK基因在内膜癌组织中的过度表达的机理需要进一步深入研究。     

Aggressive angiomyxoma of the female pelvis and perineum: a case series

Aggressive angiomyxoma (AA) was first described in 1983, and fewer than 150 cases have been reported in the world medical literature. These tumors are benign, locally infiltrative mesenchymal neoplasms with a predilection for the female pelvis and perineum and a tendency to recur. The size of AAs at presentation varies considerably; however, these tumors often achieve large dimensions before becoming clinically symptomatic. Surgical excision remains the mainstay of treatment, but whether clear, tumor-free surgical margins are necessary is controversial. We report a cohort of six patients treated surgically during the past 20 years for primary or recurrent AA. Treatment, surgical margin status, estrogen and progesterone receptor status, and outcomes are reviewed.     

不同方法治疗压褥的临床效果比较

目的探讨不同方法治疗压疮的临床效果。方法将65例患者计Ⅱ～Ⅲ度溃疡84处按基本条件相当原则随机分成A、B、C三组，A组21例28处，伤口采用清创冲洗后，给予康惠尔水胶体敷料；B组23例30处，伤口采用湿润烧伤膏；C组21例26处，清创冲洗后，给予自制凡士林油纱敷料外敷溃疡面处。结果B组愈合率最高，且平均显效时间和愈合时间均较A、C两组明显缩短，且A、B组明显高于C组。结论采用清创冲洗后用湿润烧伤膏是治疗压疮较好的方法之一。     

Role of Estrogens on Human Male Reproductive System

This review focuses on estrogen role on human male physiology. Biological estrogen actions on male reproductive system are summarized with particular regard to the effects of congenital estrogen deprivation in men. The effects of estrogen on spermatogenesis, hormonal secretion and gonadotropin feedback and on sexual behavior are discussed. It is remarked that the role of estrogens in male reproduction is a very recent acquisition in reproductive endocrinology, but it promises new future fields of research to be investigated as well as the possible disclosure of new strategies in clinical practice.     

利浦松软膏防治烧伤创面瘢痕120例疗效观察

目的 :观察利浦松软膏防治烧伤创面瘢痕的疗效。方法 :将利浦松软膏涂擦于烧伤愈合创面 ,观察瘢痕生长情况。结果 :12 0例烧伤病人创面显效 32例 ,有效 72例 ,无效 16例。结论 :利浦松软膏用于防治烧伤创面瘢痕疗效显著 ,方法简单 ,便于推广应用     

克罗米酚对血清雌激素和孕激素水平的影响

目的探讨克罗米酚(CC)在诱发排卵过程中对雌、孕激素水平的影响。方法采用放射免疫法,测定16例不明原因不孕妇女在自然周期和用药周期的排卵前1天和排卵后10天血清雌二醇(E2)和孕酮(P)浓度。结果排卵前1天和排卵后10天,用药周期的E2、P浓度均高于自然周期(P<0.05)。结论克罗米酚在诱发排卵过程中可导致内源激素平衡紊乱。     

绝经妇女激素替代治疗的困境与中医药治疗更年期综合征的前景

随着妇女平均寿命的延长，绝经后的问题日益受到全社会的广泛关注，旨在改善妇女生活质量的激素替代治疗(HRT)所引发的问题也不断涌现。2002年7月，美国国立卫生研究院心脏、肺和血液研究所宣布。妇女健康初始行动(WHI)在健康绝经后妇女中使用雌激素加孕激素的临床试验由于乳腺癌发病相对风险增加26％，且并无总体益处予以提前终止。在上述对HRT弊端新认识的背景下，中医中药面临着前所未有的走向世界的新契机。大量临床和实验研究证明，中医中药对更年期综合征引起的各种症状有较好的治疗效果，而且副作用少，因此中医辨证治疗有望替代或补充雌激素疗法对更年期综合征的治疗作用，最大限度地减少完全用雌激素替代治疗所引起的副作用。     

Neuroprotective effect of estrogen after chronic spinal cord injury in ovariectomized rats

BACKGROUND: At present, there is still lack of effective drugs for chronic spinal cord injury, whereas it is found recently that estrogen has a neuroprotective effect on brain and spinal cord injuries. OBJECTIVE: To observe the effect of estrogen on the apoptosis of nerve cells after gradual chronic spinal cord injury in ovariectomized rats. DESIGN: A randomized controlled animal trial. SETTING: Institute of Orthopaedics, the Second Hospital of Lanzhou University. MATERIALS: Sixty-five female Wistar rats of common degree, weighing 220–250 g, were provided by the experimental animal center of Lanzhou University. The rats were randomly divided into sham-operated group (n =5), estrogen-treated group (n =30) and saline control group (n =30), and the latter two groups were observed at 1, 3, 7, 14, 28 and 60 days respectively, and 5 rats for each time point. METHODS: All the rats were treated with bilateral oophorectomy 2 weeks before the experiment. T10 vertebral lamina was revolved into using plastic screw. The spinal canal impingement was not induced initially. After that, the original incision was opened to expose the screw every 7–10 days. MAIN OUTCOME MEASURES: The apoptosis and Caspase-3 positive cells in the damaged spinal cord were detected using terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end labeling (TUNEL) method and Caspase-3 immunohistochemical staining at 1, 3, 7, 14, 28 and 60 days after chronic spinal cord injury respectively. RESULTS: Totally 65 rats were used, and the deleted ones during the experiment were supplemented by others. Changes of Caspase-3 expression after spinal cord injury: In the sham-operated group, only a small amount of Caspase-3 proteins were observed in the rat spinal cord, mainly located in motor neurons of spinal cord anterior horn. In the estrogen-treated group and saline control group, positive cells expressed occasionally at 1 day postoperatively, began to increase obviously at 7 days after injury, strongly expressed at 14 and 28 days, but decreased at 60 days, mainly located in the neurons of spinal cord gray matter anterior horn, and they expressed fewer in the motor neurons and white matter of ventral horn, and there were obvious differences between the estrogen-treated group and saline control group at 7, 14, 28 and 60 days (P < 0.05). CONCLUSION: Estrogen can reduce the apoptosis of nerve cells and promote the recovery of neurological function following gradual chronic spinal cord injury.