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81.
目的探讨2007-2009年全国各级性病检验实验室沙眼衣原体(Ct)抗原检测室间质量评价结果,以利提高性病临床实验室Ct检测水平.方法发放Ct质控标本2007年76份,2008年168份,2009年175份.要求各性病实验室在规定的时间内进行检测,并将检测结果回报中国疾病预防控制中心性病控制中心参比实验室.性病控制中心对各实验室的结果进行统计分析,最后将结果反馈至各实验室.结果参评实验室Ct检测合格率从2007年的84.93%提高到2009年的92.14%,优秀率从2007年47.95%提高到2009年的70%,不合格率则从2007年的5.48%下降到2009年的0.71%.检测结果还显示,省级中心实验室和自愿参加的各级性病实验室Ct检测能力普遍较高,少数国家级性病监测点实验室检验水平有待提高.结论室间质量评价表明,各级性病实验室的检测能力及考核成绩在不断提高.  相似文献   
82.
Chlamydia trachomatis (CT) is the most prevalent sexually transmitted bacterial pathogen worldwide and causes severe reproductive tract infections. Currently, nucleic acid amplification tests (NAATs) are the gold standard for clinical diagnosis, but most NAATs are labor intensive and limited to specific CT serovars. We developed and validated a quantitative polymerase chain reaction (qPCR) assay that reproducibly detected CT serovars D, E, F, Ia, and Chlamydia muridarum over a linear range of 2 log10 to 10 log10 genomes with low coefficients of variation from both experimental and human urine samples. CT DNA loads from human vaginal, endocervical, and male urethral swabs correlated well with the BD ProbeTec ET assay (Becton Dickinson Diagnostic Systems, Franklin Lakes, NJ) run in parallel. In a preclinical microbicide evaluation, C. muridarum DNA loads in mouse swabs and tissues correlated well with an immunofluorescence assay. The optimized qPCR system provided enhanced sensitivity and facilitated the quantitative evaluation of clinical and experimental preclinical samples for anti-CT therapeutic and microbicide evaluation.  相似文献   
83.
目的研究孕妇沙眼衣原体(CT)和解脲支原体(UU)的感染对围产结局的影响。方法选取门诊行产前检查及住院待产的正常孕妇149例,采用ELISA方法检测母体血清中的抗UUIgG和抗CTIgG抗体,二者均未检出者作为对照组,对所有对象随访至妊娠结束,并详细记录分娩过程及新生儿情况。结果与对照组相比,UUIgG阳性组及复合阳性组[UUIgG( )且CTIgG( )]其早产、胎膜早破及死胎、畸胎的发生率具有显著性差异(P<0.05,P<0.05,P<0.05;P<0.01,P<0.05,P<0.01)。结论UU、CT感染可以引起不良妊娠结局,如早产、胎膜早破、畸胎、死胎,应积极预防和治疗。  相似文献   
84.
杨双  刘瑶 《中国热带医学》2005,5(3):495-496
目的研究CT感染与宫颈糜烂的关系及激光治疗效果。方法PCR法检测472例患宫颈糜烂分泌物的沙眼衣原体DNA,并对激光治疗后疗效进行分析。结果宫颈糜烂患CTDNA检出率较正常健康妇女高,CT阳性患宫颈糜烂相对重,对激光治疗效果较无CT感染患差。结论衣原体感染为宫颈糜烂的一个重要原因,衣原体感染影响宫颈糜烂激光治疗效果,应重视对宫颈糜烂患衣原体感染的检查。  相似文献   
85.
目的对肺炎衣原体AR39的RNase H IIa(CpRNase H IIa)进行克隆、表达和性质鉴定。方法按已知的CpRNase H IIa基因设计引物、扩增。用pET表达系统(表达载体、大肠杆菌BL21和N i-NTA树脂)构建重组质粒,表达和纯化CpRNase H IIa。5’3-2P标记底物RNA/DNA,鉴定酶学性质。结果CpRNase H IIa能切割RNA/DNA杂合体的寡聚核糖核苷酸链,产生3’羟基和5’磷酸基团的末端。CpRNase H IIa对Mg2 和Mn2 表现出明显的离子偏好性,且反应最适pH值为9~10。CpRNase H IIa切割12个碱基对的RNA/DNA杂合体底物的多个位点,但主要切割位点位于c10-g11之间与u7-g8之间。结论CpRNase H IIa可以有效的降解RNA/DNA杂合体的RNA链,于肺炎衣原体内担负着一定的生物学功能。  相似文献   
86.
目的探讨肺炎农原体(Chlamydia pneumoniae,Cpn)在体外连续扩增的方法。方法通过Cpn感染HEp-2细胞,采用高低速反复离心纯化的方法。并经Giemsa染色及DNA聚合酶链式反应(polymerase chain reaction,PCR)检测肺炎农原体的DNA。结果cpn感染HEp-2细胞可见包涵体形成,Cpn连续传三代后,应用Cpn特异性基因片段进行检测扩增,结果均可见437bp特异性扩增阳性带。结论Cpn能有效地在体外连续扩增。  相似文献   
87.

OBJECTIVE:

To evaluate whether street youth would use a sexually transmitted disease (STD) screening program involving non-nominal, noninvasive testing of urine for Chlamydia trachomatis with hassle-free follow-up and partner self-notification.

DESIGN:

Cross-sectional pilot study in six centres frequented by street youth 13 to 25 years of age in the Regional Municipality of Ottawa-Carleton.

INTERVENTIONS:

A structured, non-nominal face-to-face interview using an 88-item questionnaire was administered by a trained research assistant. Immediate feedback was provided to participants about specific individual risk reduction strategies for high risk life styles. Each street youth provided a urine sample that was tested non-nominally for C trachomatis by polymerase chain reaction (PCR). A single dose of azithromycin therapy was provided to participants who tested positive, each of whom was requested to encourage recent sexual partners to come forward for testing and treatment.

RESULTS:

One hundred and sixty-three street youth were recruited (98 males and 65 females [male to female ratio 1.5:1]) over the four months of the study. The mean ages of participants were males 18.3±2.50 years and females 16.7±2.02 years. Ninety-two per cent (146) of all participants were sexually active and 99% of the sexually active youth (145 of 146) submitted urine samples. Urine samples were positive in 12 (8.2%) participants (seven males, five females), all of whom were asymptomatic. All those who tested positive were recruited from a single site (site specific rate 13.6%). Overall, only 25% of those tested returned spontaneously for test results; however, nine of 12 participants with positive results were treated due to investigator vigilance in locating the youth. Street youth partner self-notification resulted in five additional street youth requesting testing and treatment.

CONCLUSIONS:

Street youth participated in a STD testing program when a street friendly program and noninvasive methods were used. Although more expensive, urine PCR testing increased program acceptance by street youth compared with previous local results. Detection of C trachomatis was high in this hard-to-reach population. There is a need to address further the problem of poor return rates for results and treatment, as well as low rates of partner notification.  相似文献   
88.
Serological markers for Chlamydia pneumoniae were investigated by using the microimmunofluorescence (MIF) test in various age and patient groups in a specific area in Turkey. IgG seropositivity to C. pneumoniae was 64.3% and 18.7% in healthy adults and children, respectively. The highest positivity rate (77%) was in the 15–19 age group. Among the groups investigated, serological findings revealed a possible etiological association between C. pneumoniae and the clinical condition in the groups with acute myocardial infarction, atypical pneumoniae and chronic obstructive pulmonary disease.  相似文献   
89.
We retrospectively conducted a study of men who have sex with men who visited the Amsterdam, the Netherlands, sexually transmitted diseases clinic from January 2002 to December 2003 and had rectal Chlamydia trachomatis infections. We found that symptomatic (73%) as well as asymptomatic (43%) patients were infected with a new C. trachomatis LGV variant.  相似文献   
90.
It is well known that Chlamydia trachomatis causes acute and chronic pelvic inflammatory disease including salpingitis. We describe a case of successful pregnancy following conservative treatment of massive ascites associated with acute Chlamydia trachomatis peritonitis. In this present case, we conservatively treated a woman with acute chlamydial salpingitis accompanied with marked ascites and an adnexal mass that simulated a malignant neoplasm. Elevated CA125 and CA19-9 also suggested a malignancy at the time of diagnosis, however following treatment they decreased to below the cut-off value, and were useful in identifying the efficacy of medical treatment. The patient subsequently became pregnant after infertility treatment and underwent a normal vaginal delivery. We conclude that the possibility of Chlamydia trachomatis peritonitis should be considered when a patient presents with ascites and an adnexal mass in sexually active women. (Reprod Med Biol 2004; 3 : 217–221)  相似文献   
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