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21.
目的:探讨4种炎症因子IL-1β、IL-6、TNF-α、MCP-1在牙周炎与冠心病(CHD)相关性中的可能角色,进一步探索牙周炎和CHD的相关机制。方法:选取2015年7月至2016年5月就诊于温州市中医院口腔科及温州市人民医院心内科的129例研究对象,其中健康者39名,单纯中重度牙周炎患者35例,单纯CHD患者31例,CHD伴中重度牙周炎患者24例,采用定制的Procarta Plex多因子ELISA试剂盒检测每位受检者的血清IL-1β、IL-6、TNF-α、MCP-1含量。结果:经协方差分析排除危险因素BMI和血压的作用后,各组血清IL-1β、IL-6、TNF-α、MCP-1的差异有统计学意义(P<0.05),CHD伴中重度牙周炎组最高。除MCP-1以外,中重度牙周炎组和CHD组血清IL-1β、IL-6、TNF-α水平均高于健康组。结论:中重度牙周炎患者升高的血清IL-1β、IL-6、TNF-α水平,可能促进CHD的发生发展。 相似文献
22.
考察《伤寒杂病论》经方中配伍应用芍药的61首经方(56首原方,5首加减方)及126条相关条文。通过对经方剂量、比例、配伍、病位主症的分析,认为:经方中芍药为白芍;白芍并非补血药,其益阴补血的功效是通过配伍实现;经方中"分"为计量单位;芍药在丸散汤中的常见用量;经方中芍药主治的症状包括四肢挛急、腹痛、烦满、下利、小便不利等。与不同药物配伍,作用部位与症状随之变化,常见配伍有桂枝、附子、甘草、柴胡、黄芩、枳实、当归、茯苓等。 相似文献
23.
Walsh DS Thavichaigarn P Pattanapanyasat K Siritongtaworn P Kongcharoen P Tongtawe P Yongvanitchit K Jiarakul N Dheeradhada C Pearce FJ Wiesmann WP Webster HK 《The Journal of surgical research》2005,129(2):221-230
BACKGROUND: Severe injury is associated with changes in monocytes that may contribute to poor outcomes. Longitudinal characterization of monocyte response patterns after trauma may provide added insight into these immunological alterations. METHODS: Venous blood obtained seven times during post-injury days 1 through 13 from 61 patients with an injury severity score >20 was assessed by flow cytometry for monocytes (CD14+) expressing HLA-DR or CD71 (transferrin receptor) and for circulating levels of interleukin (IL) 1alpha, IL-1beta, IL-6, soluble CD14 (sCD14), tumor necrosis factor-alpha (TNF-alpha), prostaglandin E(2) (PGE(2)), thromboxane B(2) (TXB(2)), and endotoxin. Urine neopterin was measured by high-pressure liquid chromatography, expressed as a neopterin-creatinine ratio. RESULTS: Trauma patients had leucocytosis days 1 through 13, monocytosis days 5 through 13, reduced proportions of CD14+HLA-DR+ cells days 2 through 5, and elevated proportions of CD14+CD71+ cells days 1 through 13. Neopterin was elevated all days, peaking on day 10. sCD14 was elevated days 2 through 13, and there were sporadic elevations of IL-1alpha, IL-1beta, IL-6, TNF-alpha, PGE(2), TXB(2), and endotoxin. Sepsis syndrome patients (n = 6) had larger and more prolonged reductions in CD14+HLA-DR+ cells and higher neopterin values, in comparison with uneventful patient outcomes. CONCLUSIONS: Altered proportions of monocytes expressing HLA-DR and CD71 and elevated sCD14 and urine neopterin levels, for up to 2 weeks after severe injury, underscores an extended period of profound immunological effects. Additional studies to more fully assess temporal monocyte response patterns after severe injury, including activation, may be warranted. 相似文献
24.
目的 对英文版感染预防和控制护理缺失量表进行汉化,为评估护士在医院感染预防和控制活动上的缺失提供有效的测量工具。方法 按照Brislin原则对英文版量表进行翻译,通过专家咨询和预试验进行跨文化调适后,并采用便利抽样法选取450名护士进行问卷调查。通过经典测量理论和项目反应理论的Rasch模型对问卷进行信效度检验。结果 中文版感染预防和控制护理缺失量表由分量表B(31个条目,感控护理活动缺失的类型及频率)和分量表C(20个条目,感控护理缺失的原因)组成,分量表总的Cronbach′s α系数分别为0.973和0.902,重测信度分别为0.917和0.957,内容效度(S-CVI)分别为0.973和0.949。Rasch分析中残差主成分分析显示分量表B符合单维性,分量表C非单维性,其4个维度符合单维性;分量表B、C的项目信度分别为0.97和0.98,分离指数分别为5.27和7.20,样本信度分别为0.85和0.88,分离指数分别为2.42和2.73。结论 中文版感控护理缺失量表信效度较好,可作为评估护士的感控护理缺失现状及分析缺失原因的测量工具。 相似文献
25.
V. R. Monger J. A. Stegeman K. Dukpa R. B. Gurung W. L. A. Loeffen 《Transboundary and Emerging Diseases》2016,63(6):e211-e218
Control and eradication of classical swine fever (CSF) in countries with a high proportion of backyard holdings is a challenge. Conventional attenuated Chinese C‐strain vaccines, though safe and effective, are difficult to use in backyard farms due to various practical reasons. The aim of this study was to evaluate the efficacy of the CSF oral bait vaccine in village backyard pig farms and to assess the farmers' knowledge on CSF and motivation on using oral vaccines. The pigs were fed the bait by the farmers themselves; one bait was given on day 0, followed by second bait on the next day. Seventy‐three per cent (140 of 193 pigs) of vaccinated pigs had either a slight (2‐fold–3‐fold; 60 pigs) or significant (at least 4‐fold; 80 pigs) increase of the antibody titre against CSFV. A significant increase of the antibody titres was mainly observed in pigs with no pre‐vaccination titre (OR = 12, 95% CI = 4–40). The number of pigs with protective antibody titres (≥40) rose from 47 (24%) to 115 (60%) following vaccination. Only 30% of the farmers claimed to be familiar with CSF, although clinical signs they mentioned were rather unspecific and could relate to many other pig diseases. Most of the farmers claimed to be motivated to use oral vaccines if made available. The oral vaccine could be a substitute for the conventional attenuated CSF vaccines in areas where it is logistically difficult for veterinarians to visit. It may therefore be a useful tool to combat endemic CSF disease in regions where the disease continues to have a serious impact on the backyard farmers who depend on pig farming for their sustenance and livelihoods. 相似文献
26.
王远 《南通大学学报(哲学社会科学版)》2015,(4)
莎拉·布莱曼是当代著名跨界音乐女高音歌唱家之一。她以独特的演唱风格和舞台艺术魅力风靡世界,推动了西方古典音乐传播。文章着眼于她独特的演唱风格和舞台艺术表演,从跨界音乐的角度入手,分析并诠释了诸多西方古典声乐作品。这些声乐作品在她的演唱下由本位的高深难懂,转为通俗化被广大听众所接受。在西方古典音乐传播的过程和流行音乐商业化不断发展的当代环境下,她的演唱形式开拓了一条大众审美和社会音乐需求的西方古典音乐传播道路。 相似文献
27.
目的 探讨急性冠脉综合征(acute coronary syndrome, ACS)早期单核细胞亚群及其趋化因子即单核细胞趋化蛋白-1 (monocyte chemoattractant protein, MCP-1)和不规则趋化因子(fractalkine, FKN)的表达特点,并分析其相关性。方法 选取我院2016年9月至12月以胸痛症状入院拟行冠脉造影术(coronary angiography, CAG)的患者。手术当天术前抽取静脉血,采用流式细胞术检测外周血单核细胞(monocyte, Mon) 3个亚型的含量及其比例,依据分化抗原-14 (cluster differentiation-14, CD-14)和CD16表达分为3个亚型即CD14+CD16-Mon (Mon1)、CD14+CD16+Mon (Mon2)和CD14-CD16+Mon (Mon3);手术当天术前及术后一天抽取静脉血,ELISA检测Mon1的趋化因子MCP-1和Mon3的趋化因子FKN水平,比较不同组MCP-1-Mon1和FKN-Mon3水平变化,并分析其相关性。结果共入选70例患者,结合其临床症状、心肌标志物、心电图、CAG检查结果进行诊断分组:急性心肌梗死(acute myocardium infarction, AMI)组患者30例、不稳定性心绞痛(unstable angina pectoris, UAP)组患者25例、CAG完全正常者(对照组) 15例。流式细胞术结果显示AMI组Mon1所占比例高于UAP组和正常对照组(P<0.05),Mon3在各组间尚无差异。AMI组患者外周血Mon3/Mon1比值低于对照组(P<0.05)。AMI组和UAP组患者FKN、MCP-1和红细胞分布宽度均高于对照组,并且FKN和Mon3具有强相关性(P<0.05;R=0.650 2)。结论 单核细胞亚群(Mon1和Mon3)在ACS早期水平增高,并伴有其负责招募的趋化因子(MCP-1和FKN)增加,且FKN和Mon3具有强相关性,提示MCP-1 Mon1和FKN-Mon3两条通路可能参与患者ACS早期病理生理过程。 相似文献
28.
Stephan M Conrad S Eggert T Heuer R Fernandez S Huland H 《The Journal of urology》2002,167(3):1497-1502
PURPOSE: To find a potential prognostic marker of the induction of hydronephrotic atrophy in congenital hydronephrosis we investigated whether the messenger (m)RNA expression and urinary concentration of monocyte chemoattractant protein-1 (MCP-1) correlated with the degree of partial ureteral obstruction, and subsequent hydronephrotic atrophy and interstitial fibrosis. MATERIALS AND METHODS: We created left partial ureteral obstruction in 96 juvenile Wistar rats and complete ureteral obstruction in 18, while 16 underwent sham operation. Depending on excretion of contrast medium into the renal pelvis after 3 days we defined 2 degrees of hydronephrosis. Renal mRNA expression of MCP-1, and renal pelvic and bladder urinary concentrations of MCP-1 were measured after 1, 2 and 3 weeks, and compared with the degree of hydronephrotic atrophy. RESULTS: Grade 1 partial ureteral obstruction resulted in mild histological changes. Grade 2 partial and complete obstruction resulted in significant hydronephrotic atrophy. MCP-1 mRNA expression in the kidney remained unchanged in grade 1 partial obstruction but was moderately increased in grade 2 partial obstruction and clearly over expressed in complete ureteral obstruction. The renal pelvic urinary concentration of MCP-1 was not higher in rats with grade 1 partial obstruction than in sham operated animals but it was significantly increased in those with grade 2 partial and complete obstruction. CONCLUSIONS: mRNA expression and the urinary concentration of MCP-1 correlate with the degree of obstruction and subsequent renal damage in hydronephrosis. They may serve as prognostic markers in children with congenital hydronephrosis. 相似文献
29.
30.
Mahmoud Sadeghi Volker Daniel Cord Naujokat Jan Schmidt Arianeb Mehrabi Martin Zeier Gerhard Opelz 《Clinical transplantation》2010,24(3):415-423
Sadeghi M, Daniel V, Naujokat C, Schmidt J, Mehrabi A, Zeier M, Opelz G. Decreasing plasma soluble IL‐1 receptor antagonist and increasing monocyte activation early post‐transplant may be involved in pathogenesis of delayed graft function in renal transplant recipientsClin Transplant 2010: 24: 415–423. © 2009 John Wiley & Sons A/S. Abstract: Delayed graft function (DGF) increases the risk of acute allograft rejection and may affect long‐term graft survival. We compared pre‐transplant, early post‐transplant, and late post‐transplant serum creatinine (Cr) and plasma levels of neopterin, cytokines, and cytokine receptors/antagonists in patients with DGF (n = 39), slow graft function (SGF) (n = 43), or immediate graft function (IGF) (n = 30). Three and eight days post‐transplant, plasma neopterin (p < 0.001; p < 0.001), Soluble Interleukin‐6 (IL‐6) receptor (R) (p = 0.002; p = 0.001), and IL‐10 (p = 0.003; p = 0.001) were higher in DGF than IGF patients. One month post‐transplant, plasma neopterin (p < 0.001) and IL‐10 (p < 0.001) were higher in DGF than IGF patients. Three days post‐transplant, the results indicated reduced sIL‐1 receptor antognist (RA) production in DGF patients (p = 0.001). Simultaneously, plasma sIL‐6R and IL‐10 increased in DGF (p < 0.001; p = 0.003) and SGF (p = 0.007; p = 0.030) patients, indicating increased production of sIL‐6R and IL‐10. Lower sIL‐1 production in DGF than IGF patients early post‐transplant might promote the increased production of monocyte‐derived neopterin, sIL‐6R, and IL‐10. This monocyte/macrophage activation might induce inflammation in the graft and subsequently cause an impairment of graft function. Blocking of monocyte activity after renal transplantation may be considered a potential approach for improving graft outcome. 相似文献