Long-term effects of propolis on serum biochemical parameters of rainbow trout (Oncorhynchus mykiss)

Long-term effects of propolis administration on serum biochemical parameters of rainbow trout (Oncorhynchus mykiss) were investigated. To determine the possible toxicity and side effects of propolis, fish were fed on diets containing 0, 0.5, 1.5, 4.5 and 9 g propolis/kg diet for 8 weeks. At the end of the experiment, various seric biochemical parameters were determined. Our results showed that all dosages induced no significant alterations in growth parameters and the seric levels of total protein, albumin, globulin, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides and activities of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, alkaline phosphatase and lactate dehydrogenase, when compared to the control group. On the basis of our findings, propolis is a non-toxic substance for rainbow trout and its long-term administration might not have any side effects.     

蜂胶水提液对体外培养的血管内皮细胞凋亡的影响

目的：探讨泰山蜂胶水提液对一定浓度的肿瘤坏死因子-α诱导的人脐静脉内皮细胞凋亡的作用。方法:用消化灌注法收集人脐静脉内皮细胞进行体外原代培养，用终浓度为50 μg/L的肿瘤坏死因子-α诱导其凋亡，培养液中分别加入终浓度为50 mg/L、100 mg/L和200 mg/L的蜂胶水提液干预，共同孵育24h，用流式细胞仪和缺口末端标记技术TUNEL检测细胞凋亡率。结果:模型组内皮细胞凋亡率均明显高于对照组，不同浓度蜂胶组内皮细胞凋亡率明显低于模型组（P＜0.01）。结论:泰山蜂胶水提液能抑制肿瘤坏死因子-α诱导的人脐静脉内皮细胞的凋亡，从而起到保护血管内皮细胞的作用。     

比较矿物三氧化物凝聚体及山东蜂胶乙醇提取物对牙髓成纤维细胞生物学性能的影响

目的 比较矿物三氧化物凝聚体(mineral trioxide aggregate,MTA)与山东蜂胶乙醇提取物对牙髓成纤维细胞活性、矿化和趋化能力以及抗炎效应的影响。方法 利用CCK-8法检测山东蜂胶提取物和MTA浸出液作用牙髓成纤维细胞1、5、7、9 d时的细胞毒性,各组同时作用15 h,Transwell法检测不同组的迁移细胞数目。矿化诱导21 d后,各组进行茜素红染色比较诱导硬组织沉积能力。按照1 mg/L浓度将脂多糖(lipopolysaccharide,LPS)加入两种材料后刺激细胞3 h,采用实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法检测不同组细胞肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)和白细胞介素-6(interleukin-6,IL-6)三种炎症因子的表达量。采用单因素方差分析及非参数检验对组间差异进行分析(P<0.05)。结果 蜂胶的细胞毒性大于MTA,蜂胶组迁移细胞数目(26.67±2.52)明显少于对照组(61.33±4.93)及MTA组(80.00±2.65),茜素红染色显示蜂胶组相较MTA组的钙沉积更多。LPS刺激细胞3 h后,蜂胶相比MTA可以显著降低IL-1β和IL-6的表达量。结论 山东蜂胶相比MTA对牙髓细胞有一定的细胞毒性,对细胞迁移的促进作用不明显,但蜂胶具有良好的抗炎效果及诱导牙髓细胞成牙本质分化的能力,经处理后有望应用于感染牙髓的活髓保存治疗。     

Propolis induces cell cycle arrest and apoptosis in human leukemic U937 cells through Bcl-2/Bax regulation

We investigated mechanism(s) where propolis induces apoptosis in human leukemic U937 cells. Propolis inhibited the proliferation of U937 cells in a dose-dependent manner by inducing apoptosis and blocking cell cycle progression in the G2/M phase. Western blot analysis showed that propolis increases the expression of p21 and p27 proteins, and decreases the levels of cyclin B1, cyclin A, Cdk2 and Cdc2, thereby contributing to cell cycle arrest. DAPI staining assay revealed typical morphology features of apoptotic cells. Propolis-induced apoptosis was also confirmed by assays with annexin V-FITC, PI-labeling and DNA fragmentation assay. The increase in apoptosis level induced by propolis was associated with down-regulation of Bcl-2 and activation of caspase-3, but not with Bax. These results suggests that propolis-induced apoptosis is related to the selective activation of caspase-3 and induction of Bcl-2/Bax regulation.     

Water-soluble derivative of propolis mitigates scopolamine-induced learning and memory impairment in mice

The water-soluble derivative of propolis (WSDP) was prepared from fresh Chinese propolis. Its major constituents were identified by high performance liquid chromatography (HPLC) analysis. It has been reported that propolis possessed a broad spectrum of biological activities but including few studies on learning and memory by now. Thus, this study was aimed to investigate the effect of WSDP on scopolamine-induced learning and memory impairment in mice. WSDP (50 mg/kg, 100 mg/kg) was given by intragastric administration (i.g.) 40 min prior to the intraperitoneal (i.p.) injection of scopolamine (1 mg/kg).The effect on amnesia was investigated with both hidden-platform acquisition training and probe trial testing in Morris water maze test. The results from 100 mg/kg WSDP group showed significant mitigation scopolamine-induced amnesia in mice. Furthermore, WSDP's effect on the acetylcholinesterase (AChE) activity in the cerebral cortex and hippocampus was also assayed. As a result, WSDP (100 mg/kg) significantly inhibited AChE activity in the hippocampus of scopolamine-treated mice. These results indicated that WSDP may mitigate amnesia in vivo through inhibition of AChE activity in the hippocampus, which suggested propolis may have potential as a pharmaceutical of brain protection with elderly population for preventing Alzheimer's disease (AD) and other neurodegenerative diseases.     

蜂胶除蜡工艺对黄酮类成分含量影响的实验研究

目的 蜂胶除蜡后对蜂胶中黄酮类化合物含量的影响. 方法 选用紫外分光光度法,以芦丁为对照品,测定总黄酮含量. 结果蜂胶除蜡后,有8.5%的总黄酮类成分损失,但胶层与水层中总黄酮量仍大于未除蜡蜂胶的总黄酮量. 结论除蜡对蜂胶中总黄酮量影响不大,在其治疗心脑血管疾病复方制剂开发应用时可作为杂质除去.     

蜂胶银杏提取物对高血脂人群血脂的影响

目的研究蜂胶银杏提取物对高血脂患者血脂的影响。方法将106名成年高血脂患者随机分为试验组与对照组,试验组服用蜂胶银杏提取物,对照组服用安慰剂,连续45 d。食试验开始及结束时进行相关安全性指标和功效指标测定。结果试验组试验后CHOL、TG与试验前比较,差异有统计学意义(P0.05),且试验后CHOL、TG与试验前比较分别下降10.25%、19.77%;试验后试验组CHOL、TG与试验前及对照组试验后比较,差异有统计学意义(P0.05);试验后试验组HDL-C与对照组比较差异无统计学意义(P0.05)。结论蜂胶银杏提取物具有降低高血脂患者血脂的功能。     

蜂胶毛胶中总黄酮、高良姜素和白杨素含量分析

目的：建立蜂胶毛胶中总黄酮以及高良姜素、白杨素含量测定方法,测定不同基原毛胶中上述各指标的含量。方法：以芦丁为对照品,采用uV法测定总黄酮含量,检测波长为415nm;用HPLC同时测定高良姜素、白杨素含量,采用Welchrom C18柱,以甲醇-0．15％磷酸溶液梯度洗脱,检测波长为268nm。结果：总黄酮在0～1．248mg线性关系良好,r=0．9999（n=6）,平均回收率为98．82％,RSD=1．45％。高良姜素、白杨素分别在0．089～0．4450,0．115～0．580μg线性关系良好,r均达到1．000,平均回收率分别为99．03％、99．32％,RSD分别为1．10％、1．72％。结论：该方法准确、可靠、简便易行,可用于蜂胶毛胶的定量分析,为蜂胶毛胶、其提取物以及蜂胶制品的质量评价和控制提供了依据。     

蜂胶及自制蜂胶奥硝唑合剂对人牙龈成纤维细胞的毒性作用

目的:了解蜂胶及自制蜂胶奥硝唑合剂对人牙龈成纤维细胞的毒性作用,为蜂胶及合剂的临床推广应用提供理论依据.方法:人牙龈成纤维细胞在不同浓度的蜂胶、奥硝唑和自制蜂胶奥硝唑合剂中体外培养24 h,用MTT法测定细胞相对增殖率(RGR);选择细胞增殖率约为50%的药物浓度组,重新培养细胞24 h,更换成不含药物的培养液连续培养7 d,每天用MTT法测定吸光度值,了解细胞增殖回复情况.结果:0.5g/L蜂胶组的RGR达到93.9%,与蜂胶其他2组间的差别有统计学意义(P<0.05);奥硝唑溶液加入蜂胶后,对应浓度组比较,两合剂组的平均吸光度值均大于奥硝唑组,其中当奥硝唑浓度为0.4g/L和0.8g/L时,差别有统计学意义(P相似文献